Biological Production Research Articles

Use of acetate as substrate for sustainable production of homoserine and threonine by Escherichia coli W3110: a modular metabolic engineering approach

Acetate, a promising yet underutilized carbon source for biological production, was explored for the efficient production of homoserine and threonine in Escherichia coli W. A modular metabolic engineering approach revealed the crucial roles of both acetate assimilation pathways (AckA/Pta and Acs), optimized TCA cycle flux and glyoxylate shunt activity, and enhanced CoA availability, mediated by increased pantothenate kinase activity, for efficient homoserine production. The engineered strain W-H22/pM2/pR1P exhibited a high acetate assimilation rate (5.47 mmol/g cell/h) and produced 44.1 g/L homoserine in 52 h with a 53% theoretical yield (0.18 mol/mol) in fed-batch fermentation. Similarly, strain W-H31/pM2/pR1P achieved 45.8 g/L threonine in 52 h with a 65% yield (0.22 mol/mol). These results represent the highest reported levels of amino acid production using acetate, highlighting its potential as a valuable and sustainable feedstock for biomanufacturing.

Evaluating the relationship between health-related social needs, patient demographics, and access to biologics in patients with moderate-to-severe asthma in Bronx, NY

Objectives This study assesses the relationship between patient age, gender, race, socioeconomic status, social determinants of health (SDoH), and access to biologics (products isolated from natural sources that target specific molecules, proteins, and cells) in patients with moderate-to-severe asthma in Bronx, NY. Methods Cohort of 289 patients with moderate-to-severe asthma treated at Montefiore Medical Center (MMC) from 2018 to 2020 was used. Patient demographics, self-reported social needs, and neighborhood socioeconomic characteristics were analyzed. Neighborhood socioeconomic status was estimated by determining median income in patients’ residential zip codes using 2020 Census data and grouping patients based on whether neighborhood median income was above or below New York State (NYS) median ($71,117/year). Area Deprivation Index tool (ADI) was used as an additional measure of neighborhood socioeconomic status. Results Patients living in regions with incomes below NYS median found to have longer wait times between biologic approval to administration than patients living in regions above median income (p = 0.012). Mean time from insurance approval to biologic administration was significantly different between Black and Latinx patients (p = 0.009). No significant difference found for patient regional income status and time from biologic prescription to approval. No significant differences in access to biologics were found for age, gender, number of health-related social needs, or patient ADI quartile. Conclusions Patients who live in areas of NYC where median income is below NYS median are more likely to experience delays in access to biologics, specifically due to time between approval and administration of medication.

Chemical composition, source characteristics, and hygroscopic properties of organic-enriched aerosols in the high Arctic during summer

Arctic regions are extremely sensitive to global warming. Aerosols are one of the most important short-lived climate-forcing agents affecting the Arctic climate. The present study examines the summertime chemical characteristics and potential sources of various organic and inorganic aerosols at a Norwegian Arctic site, Ny-Ålesund (79°N). The results show that organic matter (OM) accounts for 60 % of the total PM10 mass, followed by sulfate (SO42−). Water-soluble organic carbon (WSOC) contributes 62 % of OC. Photochemical processes involving diverse anthropogenic and biogenic precursor compounds are identified as the major sources of WSOC, while water-insoluble organic carbon (WIOC) aerosols are predominantly linked to primary marine emissions. Despite being a remote pristine site, the aerosols show a sign of chemical aging, evidenced by a significant chloride depletion, which was about 82 % on average during the study period. Nitrogen-containing aerosols are likely stemming from migratory seabird colonies and local dust sources around the sampling site. While biogenic, crustal, and sea salt-derived SO42- account for 37%, 8%, and 5% respectively, the remaining 50% is attributed to anthropogenic SO42-. Through chemical tracers, Pearson correlation coefficient matrix, and Hierarchical Cluster Analysis (HCA), the present study identifies soil biota (terrestrial biogenic) and marine emissions, along with their photochemical oxidation processes, as potential sources of Arctic aerosols during summer, while biomass burning and combustion-related sources have a minor contribution. The chemical closure of hygroscopicity highlights that while organics predominantly control aerosol hygroscopicity in the Arctic summer, specific inorganic components like (NH4)2SO4 can significantly increase it on certain days, affecting aerosol-cloud interactions and climate processes over the Arctic during summer. The present study highlights the high abundance of organics and their vital role in the Arctic climate during summer when natural aerosols are conquered.

Multi-cell-line learning for the data-driven construction of mechanistic metabolic models.

Mammalian cells are commonly used as hosts in cell culture for biologics production in the pharmaceutical industry. Structured mechanistic models of metabolism have been used to capture complex cellular mechanisms that contribute to varying metabolic shifts in different cell lines. However, little research has focused on the impact of temporal changes in enzyme abundance and activity on the modeling of cell metabolism. In this work, we present a framework for constructing mechanistic models of metabolism that integrate growth-signaling control of enzyme activity and transcript dynamics. The proposed approach is applied to build models for three Chinese hamster ovary (CHO) cell lines using fed-batch culture data and time-series transcript profiles. Leveraging information from the transcriptome data, we develop a parameter estimation approach based on multi-cell-line (MCL) learning, which combines data sets from different cell lines and trains the individual cell-line models jointly to improve model accuracy. The computational results demonstrate the important role of growth signaling and transcript variability in metabolic models as well as the virtue of the MCL approach for constructing cell-line models with a limited amount of data. The resulting models exhibit a high level of accuracy in predicting distinct metabolic behaviors in the different cell lines; these models can potentially be used to accelerate the process and cell-line development for the biomanufacturing of new protein therapeutics.

Clinical Pharmacology Strategies for Bispecific Antibody Development: Learnings from FDA-Approved Bispecific Antibodies in Oncology.

Bispecific antibodies, by enabling the targeting of more than one disease-associated antigen or engaging immune effector cells, have both advantages and challenges compared with a combination of two different biological products. As of December 2023, there are 11 U.S. Food and Drug Administration-approved BsAb products on the market. Among these, 9 have been approved for oncology indications, and 8 of these are CD3 T-cell engagers. Clinical pharmacology strategies, including dose-related strategies, are critical for bispecific antibody development. This analysis reviewed clinical studies of all approved bispecific antibodies in oncology and identified dose-related perspectives to support clinical dose optimization and regulatory approvals, particularly in the context of the Food and Drug Administration's Project Optimus: (1) starting doses and dose ranges in first-in-human studies; (2) dose strategies including step-up doses or full doses for recommended phase 2 doses or dose level(s) used for registrational intent; (3) restarting therapy after dose delay; (4) considerations for the introduction of subcutaneous doses; (5) body weight vs. flat dosing strategy; and (6) management of immunogenicity. The learnings arising from this review are intended to inform successful strategies for future bispecific antibody development.

New Molecules of Importance in the Prevention and Treatment of Acne: A Systematic Patent Review (2016-2020).

Acne is a highly prevalent disease that mainly affects the pilosebaceous units associated with sebaceous glands, causing inflammatory skin lesions and affecting the self-esteem, mental health, and quality of life of those who suffer from this disease. Different treatments exist today to prevent, reduce, and improve symptoms; however, over the years, there have been problems with bacterial resistance and slight effectiveness with prolonged use. The purpose of this article is based on the review of patents of new products of principal topical administration for the treatment of acne in recent years 2016-2020, to evaluate and analyze novel synthetic molecules and semi-synthetics with potential therapeutic and preventive in the acne treatment. A systematic review of patents was conducted through the official database of the European Patent Office - Espacenet, where the search focused on the keywords: "acne and bacteria" in the title or abstract. Only patents granted between the years 2016-2020 were included, with products having molecules with a synthetic and semi-synthetic origin, without considering natural, biological products or those used as diagnostic means. A total of 19 patents were selected, most with principally antimicrobial and antiinflammatory action, where the reduction in the appearance of resistance by C. acnes is verified, and its action is complemented by inhibiting the different pathophysiological mechanisms that lead to the worsening of the disease. Novel approaches in the treatment and prevention of acne, mainly topically, are focused on the reduction of bacterial resistance and irritation compared to current treatments. The use of combined formulations provides better results with additional benefits, improving treatment times and patient adherence.

Production and characterization of biologicals for disease diagnosis and pathological evaluation of elephant endotheliotropic herpesvirus (EEHV)

Elephant endotheliotropic herpesviruses (EEHV) belong to the family Herpesviridae and cause a highly fatal hemorrhagic infection in elephants. EEHV poses a global threat to the already endangered elephant population. Since EEHV is a non-cultivable virus, there is a scarcity of specific diagnostics, therapeutics, and vaccines. In this study, our objective was to develop biologicals for diagnosis and pathological studies against the most prevalent EEHV1A/1B. We expressed two truncated fragments of the DNA polymerase, glycoprotein B (gB), and glycoprotein (gL) of EEHV in the prokaryotic system. Hyperimmune serum against the purified antigens was raised in rabbits and guinea pigs. We validated the reactivity of this hyperimmune serum using western blotting, ELISA, and immune-histochemistry on known positive infected tissues. Samples collected from 270 animals across various states in India were evaluated with these biologicals. The raised antibodies successfully demonstrated virus in immune-cytochemistry. Additionally, all known positive samples consistently exhibited significant inhibition in the OD values when used in the competitive format of ELISA across all four antigens when compared to the serum collected from known negative animals. An apparent sero-prevalence of 10 % was observed in the randomly collected samples. In summary, our study successfully developed and validated biologicals that will be invaluable for EEHV diagnosis and control.

Biotrophy and Its Biological Role Productivity, Physiological and Microbial Indicators of Broiler Chickens and Layng Hens

Biotrophy and Its Biological Role Productivity, Physiological and Microbial Indicators of Broiler Chickens and Layng Hens

Quantifying the Potential for Nitrate-Dependent Iron Oxidation on Early Mars: Implications for the Interpretation of Gale Crater Organics.

Geological evidence and atmospheric and climate models suggest habitable conditions occurred on early Mars, including in a lake in Gale crater. Instruments aboard the Curiosity rover measured organic compounds of unknown provenance in sedimentary mudstones at Gale crater. Additionally, Curiosity measured nitrates in Gale crater sediments, which suggests that nitrate-dependent Fe2+ oxidation (NDFO) may have been a viable metabolism for putative martian life. Here, we perform the first quantitative assessment of an NDFO community that could have existed in an ancient Gale crater lake and quantify the long-term preservation of biological necromass in lakebed mudstones. We find that an NDFO community would have the capacity to produce cell concentrations of up to 106 cells mL-1, which is comparable to microbes in Earth's oceans. However, only a concentration of <104 cells mL-1, due to organisms that inefficiently consume less than 10% of precipitating nitrate, would be consistent with the abundance of organics found at Gale. We also find that meteoritic sources of organics would likely be insufficient as a sole source for the Gale crater organics, which would require a separate source, such as abiotic hydrothermal or atmospheric production or possibly biological production from a slowly turning over chemotrophic community.

Exploring Greater Flexibility for Chronic Toxicity Study Designs to Support Human Safety Assessment While Balancing 3Rs Considerations.

Chronic repeated-dose toxicity studies are required to support long-term dosing in late-stage clinical trials, providing data to adequately characterize adverse effects of potential concern for human safety. Different regulatory guidances for the design and duration of chronic toxicity studies are available, with flexibility in approaches often adopted for specific drug modalities. These guidances may provide opportunities to reduce time, cost, compound requirement and animal use within drug development programs if applied more broadly and considered outside their current scopes of use. This article summarizes presentations from a workshop at the 43rd Annual Meeting of the American College of Toxicology (ACT) in November 2022, discussing different approaches for chronic toxicity studies. A recent industry collaboration between the Netherlands Medicines Evaluation Board (MEB) and UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) illustrated current practices and the value of chronic toxicity studies for monoclonal antibodies (mAbs) and evaluated a weight of evidence (WOE) model where a 3-month study rather than a 6-month study might be adequate. Other topics included potential opportunities for single-species chronic toxicity studies for small molecules, peptides and oligonucleotides and whether a 6-month duration non-rodent study can be used more routinely than a 9-month study (similar to ICH S6(R1) for biological products). Also addressed were opportunities to optimize recovery animal use if warranted and whether restriction to one study only (if at all) can be applied more widely within and outside ICH S6(R1).

Caring for/with Modernist Playthings: Fidgeting with Objects in Tennessee Williams's The Glass Menagerie.

Modernist literature of the early to mid-twentieth century on both sides of the Atlantic is replete with examples of a particular kind of relationship with objects, namely, the touching, collecting, and grasping of small, often highly personal, and ostensibly quotidian objects. From John's glass collection in Woolf's "Solid Objects," Peter Walsh's stroking of his pocket-knife in Mrs. Dalloway, Miriam's frenzied absorption with flowers in Lawrence's Sons and Lovers, to Laura's fiddling of her glass menagerie in Tennessee Williams's eponymous play, fidgeting in modernist literature and drama reveals a particular tendency of not just characters' possession of things but also their possession by things. This phenomenon, I argue, allows characters to practice care as they withdraw from oppressive narratives of normalcy and (economic and biological) productivity, challenging their exclusionary and othering configurations. My paper looks at fidgeting in The Glass Menagerie as a part of this larger ideological and haptic orientation in modernist literature. The care invested by Laura in her intimate relationship with these "playthings" allows her to intercept not only male narrativizing forces and articulation of herself but also the rhetoric of productivity that circulates both within the play and in the larger economic backdrop of post-depression America. My paper attempts to foreground these objects of care in our readings of the play and modernist texts in general and, in so doing, highlight their importance as lenses of analysis that render visible alternate forms of agency and resistance. Lastly, it attempts to reframe fidgeting as an act of embodied refusal, evoking the radical potential of refusal within feminist and disability studies.

Silver nanoparticle biosynthesis utilizing Ocimum kilimandscharicum leaf extract and assessment of its antibacterial activity against certain chosen bacteria.

The use of plants in the biological production of silver nanoparticles for antibacterial applications is a growing field of research. In the current work, we formulated Ocimum kilimandscharicum extracts using silver nanoparticles, and evaluated its potential antibacterial activity. Aqueous and methanol plant extracts were used to reduce silver nitrate at different time intervals (30 to 150 minutes) and pH (2 to 11). The UV-visible absorption spectrum recorded for methanol and aqueous extracts revealed a successful synthesis of AgNPs for methanol and aqueous extracts. The antimicrobial activity of the AgNPs was evaluated against Escherichia coli ATCC 25922, Salmonella choleraesuius ATCC 10708, and Staphylococcus aureus ATCC 25923 The best inhibition zone for the methanol and aqueous-mediated AgNPs, ranging from 12 ± 1 to 16 ± 1mm. Additionally, the methanol and aqueous extract silver nanoparticles had the same Minimum Inhibitory Concentration (6.25 ± 0.00 mg/ml), whereas the Minimum Bactericidal Concentrations were 12.5 ± 0.00 and 25 ± 0.00 mg/ml, respectively. The highest inhibition zone of 16 ± 1 mm was observed against Salmonella choleraesuius with 50 ± 0.00 mg/ml aqueous silver nanoparticles. The results show that the silver nanoparticles made with Ocimum kilimandscharicum have antibacterial action against those microorganisms.

The view of synthetic biology in the field of ethics: a thematic systematic review.

Synthetic biology is designing and creating biological tools and systems for useful purposes. It uses knowledge from biology, such as biotechnology, molecular biology, biophysics, biochemistry, bioinformatics, and other disciplines, such as engineering, mathematics, computer science, and electrical engineering. It is recognized as both a branch of science and technology. The scope of synthetic biology ranges from modifying existing organisms to gain new properties to creating a living organism from non-living components. Synthetic biology has many applications in important fields such as energy, chemistry, medicine, environment, agriculture, national security, and nanotechnology. The development of synthetic biology also raises ethical and social debates. This article aims to identify the place of ethics in synthetic biology. In this context, the theoretical ethical debates on synthetic biology from the 2000s to 2020, when the development of synthetic biology was relatively faster, were analyzed using the systematic review method. Based on the results of the analysis, the main ethical problems related to the field, problems that are likely to arise, and suggestions for solutions to these problems are included. The data collection phase of the study included a literature review conducted according to protocols, including planning, screening, selection and evaluation. The analysis and synthesis process was carried out in the next stage, and the main themes related to synthetic biology and ethics were identified. Searches were conducted in Web of Science, Scopus, PhilPapers and MEDLINE databases. Theoretical research articles and reviews published in peer-reviewed journals until the end of 2020 were included in the study. The language of publications was English. According to preliminary data, 1,453 publications were retrieved from the four databases. Considering the inclusion and exclusion criteria, 58 publications were analyzed in the study. Ethical debates on synthetic biology have been conducted on various issues. In this context, the ethical debates in this article were examined under five themes: the moral status of synthetic biology products, synthetic biology and the meaning of life, synthetic biology and metaphors, synthetic biology and knowledge, and expectations, concerns, and problem solving: risk versus caution.

Drivers of organic carbon distribution and accumulation in the northern Barents Sea

Sedimentary properties and accumulation rates on the continental shelf and in the deep sea reflect temporal oceanographic, biological and chemical processes occurring in the water column and the sediment surface. We used the radionuclides 210Pb, 226Ra, and 137Cs activities to estimate sedimentation rates during the last century at nine stations in the northern Barents Sea region. Elemental (C, N) and stable isotopic composition (δ13C, δ15N) were also analysed from the nine stations sampled in August 2018, and, for five other stations sampled in August and December 2019, and in March and May 2021. Sediment accumulation rates varied between 130 and 1 410 g m−2 y−1. The < 63 μm normalized total organic carbon (TOC63) and the total nitrogen from the sediment surface varied between 0.90–2.56 % and 0.13–0.33 %, respectively. Ice-free shelf stations had higher TOC63 and possibly fresher organic matter (high δ13C, low δ15N) than ice-covered more northern stations. The opposite trend was observed for total inorganic carbon. We found that these trends in biogeochemical parameters were spatially structured by the winter sea ice concentration and biological production differences, and exhibited a south-north separation of the Polar Front region. The low and stable organic carbon accumulation rate (1.7–13.4 g Corg m-2 y−1; ARtoc) is a function of slow sedimentation rates, and high degradation and residence time in the water column and at the sediment–water interface. Overall, the ARtoc has been stable for the past 100 years, with a slight increase from the early 1970s to the present at the shelf and slope stations. Our results highlight that spatial scales of variability of the studied sedimentary parameters are linked to spatial patterns of important environmental variables (e.g., chlorophyll-a, sea ice concentration) in the region. In contrast, no seasonal differences were observed in the sediment parameters of revisited stations, and the dated sediment geochemical profiles did not exhibit substantial longer-term variation. This means that climate-induced changes in variables that modify the sedimentary geochemistry of the environment may affect benthic community activity and structure before leaving a record in ARtoc.

Online monitoring of continuous aqueous two-phase flotation (ATPF) for the development of an autonomous control strategy

Aqueous two-phase flotation (ATPF) is a separation technique for isolating biological products such as enzymes from crude complex biosuspensions. The continuous operation mode of ATPF allows high throughput while maintaining a high separation efficiency. In this work, a laboratory plant for continuous ATPF with integrated online measurement technology is presented and ATPF experiments with the model enzyme phospholipase A2 are performed. Three electrical conductivity probes allow real time and spatially resolved characterization of phase mixing in the flotation tank caused by rising bubbles. UV/Vis spectroscopy is used to measure the enzyme concentration online at the top phase outlet. The equilibrium concentrations in the top and bottom phase are linearly dependent on the feed concentration, revealing a constant partition coefficient. The experimental results of this work provide the basis for the development of an autonomous feedback controller for the continuous ATPF process.

Use of biological products on spring barley to inhibit Fusarium fungi

Use of biological products on spring barley to inhibit Fusarium fungi

Modeling dominant height growth of teak plantations in the Caribbean region of Colombia

Identifying sites with adequate biological productivity is a critical factor in ensuring timber production and the profitability of forest-based investments. The productivity of forest sites is influenced by climatic, edaphic and topographic variables, as well as by silvicultural practices. Site index is a phytocentric method widely used to assess site productivity and its estimation is based on dominant height growth modeling. Teak is the fifth most planted forest species in Colombia, and its importance is associated with high economic returns and profitability. This study aims to model dominant height growth using the generalized algebraic difference approach for teak plantations established in the Caribbean region of Colombia. The Lundqvist-Korf model, in which the correlation of the residuals was handled with a continuous autoregressive specification of the first order, resulted in a satisfactory statistical estimation of the dominant height growth. The results indicate that in the Caribbean region of Colombia, productive sites for the establishment of teak plantations can be found as productive as in some tropical American countries and better than some sites in Asian countries. This suggests a potential for the expansion of teak plantations and forest-based investments in Colombia.

Status and future developments for downstream processing of biological products: Perspectives from the Recovery XIX yield roundtable discussions.

Governments and biopharmaceutical organizations aggressively leveraged expeditious communication capabilities, decision models, and global strategies to make a COVID-19 vaccine happen within a period of 12 months. This was an unusual effort and cannot be transferred to normal times. However, this focus on a single vaccine has also led to other treatments and drug developments being sidelined. Society expects the pharmaceutical industry to provide an uninterrupted supply of medicines. However, it is often overlooked how complex the manufacture of these compounds is and what logistics are required, not to mention the time needed to develop new drugs. The overarching theme, therefore, is patient access and how we can help ensure access and extend it to low- and middle-income countries. Despite unceasing efforts to make medications available to all patient populations, this must never be done at the expense of patient safety. A major fraction of the costs in biopharmaceutical manufacturing are for drug discovery, process development, and clinical studies. Infrastructure costs are very difficult to quantify because they often depend on whether a greenfield facility or an existing, depreciated facility is used or adapted for a new product. To accelerate process development concepts of platform process and prior knowledge are increasingly leveraged. While more traditional protein therapeutics continue to dominate the field, we are also experiencing the exciting emergence and evolution of other therapeutic formats (bispecifics, tetravalent mAbs, antibody-drug conjugates, enzymes, peptides, etc.) that offer unique treatment options for patients. Protein modalities are still dominant, but new modalities are being developed that can be learned from including advanced therapeutics-like cell and gene therapies. The industry must develop a model-based strategy for process development and technologies such as continuous integrated biomanufacturing must be adopted. The overall conclusion is that the pandemic pace was unsustainable, focused on vaccine delivery at the expense of other modalities/disease targets, and had implications for professional and personal life (work-life balance). Routinely reducing development time from 10 years to 1 year is nearly impossible to achieve. Environmental aspects of sustainable downstream processing are also described.

The feasibility and effectiveness of using biological products on onions (&lt;i&gt;Allium cepa&lt;/i&gt; L.) in an annual crop

Relevanse. Rhizosphere associative bacteria, multiplying on root secretions, provide the plant with the entire complex of necessary nutrients from the soil and help increase productivity.The purpose of the research was to identify the feasibility and effectiveness of using bacterial preparations, optimizing the rates and timing of their application when cultivating onions as an annual crop on alluvial meadow soils of the Non-Chernozem Zone.Materials and Methods. Field experiments were carried out on the experimental field of VNIIO on an area of 1000 m2 in 2021-2023. We used drugs provided by the Federal State Budgetary Scientific Institution "All-Russian Research Institute of Agricultural Microbiology" (St. Petersburg): BisolbiPlant BIS88 (Bacillus pumilis strain); N40 – Experimental strain (Paenibacillus xylanexedens) – bacillary nitrogen fixer; V514 – Experimental strain (Bacillus megaterium) – bacillary stimulator of mobilization of soil phosphates and phosphorus from mineral fertilizers. The following tasks were solved by year: 2021 – Integrated use of biological products at application rates, l/ha: 0.0; 10.0; 20.0; 40.0; 2022 – Application of biological products in the following options: Control, Control + BIS88 (1+1) l/ha, Control + BIS88 (1+1) l/ha + N40 (1+1) l/ha + V514 (1+1) l/ha; 2023 – Options: BIS88 (0.5+0.5) l/ha + N40 (0.5+0.5) l/ha + V514 (0.5+0.5) l/ha at different base levels spring fertilization: N160P160K160; N110P110K110; N60P60K60.Results. It is not advisable to apply the studied bacterial preparations at a total rate of more than 10.0 l/ha during the cultivation of onions due to plant disease with bacteriosis. The use of the BisolbiPlant drug at a rate of (0.5+0.5) l/ha helps to increase the yield of onions with harvest quality comparable to the control. Biological products applied in combination with irrigation water during the growing season: BIS88 (0.5+0.5) l/ha; N40 (0.5+0.5) l/ha; V514 (0.5+0.5) l/ha, provide an increase in yield (13.4%) with harvest quality comparable to the control in the case of a low (N60P60K60) level of basic spring fertilizer application with subsequent fertilizing during the growing season Ca40N30 + N40K40 + N40K40. At the rates of the main spring application of fertilizers N110P110K110 and N160P160K160, complex application of preparations does not ensure an increase in the yield and its quality.

Improvement of millet cultivation technology in the Kabardino-Balkarian Republic

The article studies the effectiveness of using mineral fertilizers, growth stimulants and bacterial preparations in the cultivating millet technology. The research was carried out in 2021–2023 on the experimental field of the Kabardino-Balkarian Scientific Research Institute of Agriculture, which located in the steppe zone of the Kabardino-Balkarian Republic. The object of study is the mid-late millet variety Kavkazskie Zori, bred by Kabardino-Balkarian Research Institute of Agriculture. Inoculation of seeds with Rizoagrin (Agrobacterium radiobacter) and Azophyte (Azotobacter vinelandii) was carried out on the day of sowing, exogenous spraying of plants with growth stimulants Mival Agro (10 g/ha), MS-extra (0.5 kg/ha) – in the three-leaf phase. Treatment of seeds with biological preparations Rizoagrin and Azofit increases field germination by 8.9 and 5.3%, respectively, relative to the control. They also have a positive effecton the preservation of plants for harvesting. The usage of biological products provides an increase in the yield of millet by 0.38–0.54 t/hacompared to the control, fertilizers – 0.64 t/ha, growth stimulants – 0.27–0.46 t/ha. Maximum productivity was achieved with the biological product Rizoagrin and the growth stimulator MS-extra against the background of N10P26K26 – 2.48 t/ha.