Biological Activities Of Polysaccharides Research Articles

Physicochemical Properties and Biological Activities of Polysaccharides from Panax Notoginseng Separated by Fractional Precipitation.

The dried root of Panax notoginseng is a medicinal and food ingredient. P. notoginseng polysaccharides (PNPs) have physicochemical properties, which have not been fully elucidated. This study aimed to identify a method to separate the PNP fractions and investigate their activities. PNPs were prepared from roots by hot water extraction, deproteinization, and decolorization. PNP20, PNP40, and PNP60 fractions were isolated through stepwise ethanol precipitation at 20 %, 40 %, and 60 % concentrations, respectively. The three polysaccharide fractions were characterized using chromatography, spectroscopy, and thermogravimetric analysis, and their moisture retention, antioxidant, and tyrosinase-inhibition properties were evaluated. Monosaccharide composition analysis showed that the three PNPs contained mannose (Man), galacturonic acid (GalA), glucose (Glc), galactose (Gal), and arabinose (Ara) in different molar ratios. HPGPC analysis demonstrated that the polysaccharides precipitated with higher ethanol concentrations had lower molecular weights (Mw). Furthermore, all PNPs had distinct moisturizing and hygroscopic properties and antioxidant activities, with PNP60 showing better antioxidant properties and a competitive mixture of hygroscopic properties and tyrosinase inhibition. The chemical composition and structural characteristics of PNPs could affect their functional attributes. PNP60 has the potential to be a moisturizer and antioxidant and could be used in the development of cosmetic ingredients.

Research progress on extraction, separation, structure, and biological activities of polysaccharides from the genus Atractylodes: A review.

Plants of the genus Atractylodes are perennial herbaceous plants in the family Asteraceae, whose rhizome is often used in the production of medicines and health products. There are 6 main species in this genus, namely A. macrocephala, A. lancea, A. chinensis, A. carlinoides, A. koreana and A. japonica. Among them, A. lancea and A. macrocephala are the most extensively investigated. Polysaccharides as the main active ingredients extracted and isolated from plants in this genus, show good pharmacological activities in vivo and in vitro, such as immunomodulatory, antioxidant, antidiabetic and intestinal protective activities. The pharmacological activities of polysaccharides are closely related to their extraction methods and physicochemical properties. This article discusses the extraction and separation methods, molecular weight, monosaccharide composition, chemical structure characteristics and pharmacological activities of polysaccharides from the genus Atractylodes. Furthermore, a comparative analysis of the relationship of monosaccharide composition, relative molecular weight and structural modifications with the pharmacological activities of polysaccharides of the genus Atractylodes was carried out, which provided a reference for the development and utility of polysaccharides.

Relationship between the extraction methods, structural characteristics, and biological activities of polysaccharides from 5 typical polyporaceae fungi

Polyporaceae fungi are widely found in nature and have long been an important part of the field of traditional Chinese medicine field. The numerous health benefits of Polyporaceae fungi may be related to their polysaccharides, which have significant biological activities in terms of antitumor, immunomodulation, antioxidation, liver protection, and blood lipid-lowering effects. Studies have shown that different extraction methods have a significant impact on the structural characteristics and biological activity of polysaccharides. The structural characteristics of fungal polysaccharides, such as composition, molecular weight, branching degree, and glycosidic bond type, are closely related to their biological activity. This article has systematically reviewed the relationship between the extraction and purification methods, structural characteristics, and biological activities of 5 typical fungal polysaccharides, aiming to provide a scientific basis for the application of polypore fungal polysaccharides in the field of traditional Chinese medicine and lay the foundation for their further research and development.

Isolation, structural characterization and biological activities of polysaccharides from Chondrus crispus

Biomedical applications often exploit the biodegradability and biocompatibility of natural polysaccharides. In this study, polysaccharide extracts from Chondrus crispus (CC) were characterized using HPLC-SEC, FTIR and 1H NMR spectroscopy. The proliferative and migrative capacities of various extracts on RAW264.7 cells, along with their impact on nitric oxide (NO) production and phagocytosis, were assessed. Polysaccharide bioactivity was also explored using HDF and HaCaT cell lines. This study additionally investigated the anti-inflammatory effects of CC fractions on Caco-2 cells, examining their impact on cytokines (TNF-α, TGF-β1) and chemokine (MCP-1) expression. CC-derived polysaccharides effectively modulated inflammatory activity and improved scratch or exclusion wound healing. Hot (95 °C) extracted polysaccharides fraction CC-2B (λ and, κ-carrageenan) stimulated RAW264.7 cell proliferation, while cold (25 °C) extracted polysaccharides fraction CC-1A (0.5 μg/μL) inhibited cell proliferation at 24 h (69.7 ± 4.8%, p<0.001), while exhibiting increased phagocytosis activity. Furthermore, RAW264.7 cells treated with hot-extracted fraction CC-2B showed a significant reduction in LPS-stimulated NO production (p<0.0001) over 24 h. HaCaT and HDF cell proliferation were stimulated when treated with fraction CC-2B (p<0.0001). Moreover, Caco-2 cells treated with all fractions displayed enhanced TGF-β1 expression and reduced TNF-α expression at 24 h, indicating the potential of these fractions for future medical applications. Polysaccharide cold-extracted fraction (CC-1A) exhibited highly effective anticoagulant properties. All polysaccharide fractions (0.13 μg/μL) appeared to be non-cytotoxic for Caco-2 cells during 24 h. CC-derived polysaccharides have potential applications in the medical (wound healing, gastrointestinal distress), food, cosmetic, and pharmaceutical industries.

Isolation, purification, and biological activities of polysaccharides from Amorpha fruticosa flowers

The extraction, isolation, structural characterisation and biological activities of polysaccharides from Amorpha fruticosa flowers were investigated. First, the crude polysaccharide AFP was extracted, and two major purified polysaccharide fractions AFP-2 and AFP-3 were isolated. The molecular weight and monosaccharide compositions of AFP-2 and AFP-3 were determined. Then the antioxidant activities of AFP, AFP-2 and AFP-3 were assessed by DPPH radical, β-Carotene bleaching and hydroxyl radical assays. All three tested polysaccharides showed good antioxidant activity while AFP was the strongest one. The study also showed that AFP, AFP-2 and AFP-3 have good tyrosinase inhibition, moisture absorption and retention activities. The results will provide a helpful reference for the application of polysaccharide from Amorpha fruticosa flowers as a natural cosmetic ingredient.

Ultrasound-assisted complex enzyme extraction, structural characterization, and biological activity of polysaccharides from Ligustrum robustum

Ligustrum robustum is one of the traditional teas in China with a long history of drinking and medicinal use. Through Response surface optimization, the yield of polysaccharides extracted by ultrasonic-assisted complex enzyme (UAE-EN) method was increased to 14.10 ± 0.56 %. Neutral homogeneous polysaccharide (LRNP) and acidic homogeneous polysaccharide (LRAP-1, LRAP-2, LRAP-3) from L. robustum were purified. The molecular weights of them were 5894, 4256, 4621 and 3915 Da. LRNP was composed of glucose (Glc), galactose (Gal), arabinose (Ara) with molar percentage of 24.97, 42.38 and 30.80. Structure analysis revealed that the backbone of LRNP consisted of 1,5-linked α-Araf, 1,4-linked β-Galp, 1,6-linked β-Galp, and 1,4-linked β-Glcp with the branches of 1,2-linked α-Araf, 1,3-linked α-Araf, 1,3-linked β-Glcp and 1,6-linked β-Galp residues, some terminal residues of α-Araf, β-Glcp and α-Galp were also included. In vitro experiments showed that the four polysaccharides possessed excellent antioxidant, antitumor and hypoglycemic activities. LRNP possessed the protective effect against oxidative stress. The studies provide a basis for further exploitation of L. robustum.

Preparation, structural characteristics and pharmacological activity of polysaccharides from Polygala tenuifolia: A review

Polygala tenuifolia is a traditional Chinese medicine with a long history of application, with the efficacy of suppressing cough, calming asthma, tranquilizing the mind, and benefiting the intellect. It is classified as a top-quality medicine in Shennong's Classic of Materia Medica. Polysaccharide is an important active ingredient in Polygala tenuifolia, which consists of several monosaccharides, including Ara, Gal, Glc, and so on. In this review, the preparation methods, structural characteristics, and biological activities of polysaccharides from Polygala tenuifolia are summarized, and the problems in the current studies are discussed to support further research, development, and utilization.

Polysaccharides from fructus corni: Extraction, purification, structural features, and biological activities

Fructus Corni, derived from the dried fruit of Cornus officinalis Sieb. Et Zucc., is widely used as a food source and Chinese herb. Fructus Corni, as an indispensable ingredient in Liuwei Dihuang decoction, tonifies the liver and kidneys. As the main component of water decoctions, Fructus Corni polysaccharides demonstrate multifaceted effects, including hypoglycemic, hypolipidemic, antioxidant, anti-aging, sexual function regulation, and anti-epileptic, The ultrasound-assisted extraction method obtained the highest yields of Fructus Corni polysaccharides. However, it has notable shortcomings and lacks further innovation. The homogeneous polysaccharides obtained from Fructus Corni are mostly neutral polysaccharides with relatively limited structure, and the mechanism of their biological activity needs to be further elucidated. In addition, different extraction, isolation and purification methods may change the molecular weight, monosaccharide composition, and biological activity of polysaccharides. Therefore, this study systematically summarized the extraction, purification, structural features, and biological activities of Fructus Corni polysaccharides. This study aimed to provide support for the ongoing development and application of Fructus Corni polysaccharides.

Analysis and prospect of correlation between relative molecular weight and efficacy of polysaccharides from medicinal plant resources

Polysaccharides from medicinal plant resources are a kind of polymers extracted from medicinal plants. They are complex long chains formed by different monosaccharides connected via glucosidic bonds. These polysaccharides usually have straight chain and branched chain structures, and their relative molecular weight changes greatly. Modern studies have shown that the biological activi-ty of polysaccharides from medicinal plant resources is closely related to their relative molecular weight. This paper first reviewed the preparation and detection methods of polysaccharides from medicinal plant resources with different relative molecular weights. Then, the paper summarized and analyzed the general experience of the correlation between efficacy and relative molecular weight of polysaccharides from medicinal plant resources with different molecular weights. It was considered that polysaccharides with large relative molecular weights(&gt;100 kDa) play a leading role in immune regulation. Polysaccharides with medium relative molecular weights(10-100 kDa) play a leading role in immune regulation and the protection of the liver. Polysaccharides with small relative molecular weights(&lt;10 kDa) play a leading role in anti-oxidation, regulation of intestinal flora, regulation of blood glucose and lipids, anti-fatigue, and the protection of nerves. Therefore, precise development of polysaccharides from medicinal plant resources based on relative molecular weight is expected to improve their biological activity and application value.

Comparative Study on the Impact of Different Extraction Technologies on Structural Characteristics, Physicochemical Properties, and Biological Activities of Polysaccharides from Seedless Chestnut Rose (Rosa sterilis) Fruit.

Seedless chestnut rose (Rosa sterilis S. D. Shi, RS) is a fresh type of R. roxburghii Tratt with copious functional components in its fruit. Polysaccharides are recognized as one of the vital bioactive compounds in RS fruits, but their antioxidant and hypoglycemic properties have not been extensively explored. Hence, in this study, accelerated solvent extraction (RSP-W), citric acid (RSP-C), 5% sodium hydroxide/0.05% sodium borohydride (RSP-A), and 0.9% sodium chloride (RSP-S) solution extraction were individually utilized to obtain RS fruit polysaccharides. The physicochemical properties, structural characteristics, and biological activities were then compared. Results indicated that extraction methods had significant influences on the extraction yield, uronic acid content, monosaccharide composition, molecular weight, particle size, thermal stability, triple-helical structure, and surface morphology of RSPs apart from the major linkage bands and crystalline characteristics. The bioactivity tests showed that the RSP-S, which had the greatest amount of uronic acid and a comparatively lower molecular weight, exhibited more potent antioxidant and α-glucosidase inhibitory property. Furthermore, all RSPs inhibited α-glucosidase through a mixed-type manner and quenched their fluorescence predominantly via a static quenching mechanism, with RSP-S showing the highest binding efficiency. Our findings provide a theoretical basis for utilizing RSPs as functional ingredients in food industries.

Purification and structural characterization of two polysaccharides with anti-inflammatory activities from Plumbago zeylanica L.

Plumbago zeylanica L., a traditional Chinese medicine, has anti-bacterial and anti-inflammatory effects, and it is critical important to explore the chemical compounds and evaluate their biological actions from the medicinal plant. However, the chemical structure and biological activities of polysaccharides from P. zeylanica. were still poorly understood. In this study, two water-soluble polysaccharides named WPZP-2-1 and WPZP-2-2 were purified from P. zeylanica L. Chemical and spectroscopic tests showed that the main chain of WPZP-2-1 was →4)-α-D-GalpA-(1 → 2)-α-L-Rhap-(1→, and the branch chain was galactose or arabinose. The main chain of WPZP-2-2 was composed of →4)-α-D-GalpA-(1 → 2)-α-L-Rhap-(1→, and the O-2 and O-3 of →4)-α-D-GalpA had a small amount of acetylation. In addition, in vitro test showed that WPZP-2-1 and WPZP-2-2 significantly improved the inflammatory damage of LPS + IFN-γ-induced THP-1 cells via reducing the protein levels of CD14, TLR4 and MyD88, thereby promoting IL-10 expression and inhibiting the mRNA levels of TNF-α and IL-1β. Those findings indicated that WPZP-2-1 and WPZP-2-2 from the plant should be served as the potential anti-inflammatory agents.

Effects of different extraction methods on the physico-chemical characteristics and biological activities of polysaccharides from Clitocybe squamulosa

This study comparatively evaluated the effects of the commonly used six extraction methods (acidic, alkaline, enzymatic, ultrasonic, high-pressure, and microwave) on the physico-chemical properties, processing characteristics, and biological activities of polysaccharides from Clitocybe squamulosa (CSFPs). The results show that polysaccharides extracted using an enzyme-assisted extraction method has a relatively high extraction yield (4.46 ± 1.62 %) and carbohydrate content (70.79 ± 6.25 %) compared with others. Furthermore, CSFPs were all composed of glucose, galactose, mannose, xylose, and glucosamine hydrochloride. Only ultrasonic-assisted extraction of polysaccharides (CSFP-U) has a triple helix chain conformation. Scanning electron microscopy (SEM) revealed significant differences in the microstructure of polysaccharides prepared using different methods. Besides that, the polysaccharides prepared by alkali extraction (CSFP-B) and high-pressure assisted extraction (CSFP-H) have good water (2.86 ± 0.29 g/g and 3.15 ± 0.29 g/g) and oil (8.13 ± 0.32 g/g and 7.97 ± 0.04 g/g) holding properties. The rheological behavior demonstrated that CSFPs solutions were typical non-Newtonian fluid. Apart from this, the antioxidant capacity (clearing DPPH (IC50 = 0.29) and ABTS free radicals (IC50 = 0.19), total reduction ability (IC50 = 3.02)) of polysaccharides prepared by the microwave-assisted extraction (CSFP-M) method was significantly higher than that of other extraction methods. By contrast, the polysaccharide prepared by acid extraction (CSFP-A) has the optimum binding capacity (bile acid salt (71.30 ± 6.78 %) and cholesterol (57.07 ± 3.26 mg/g)). The antibacterial activity of CSFPs was positively correlated with their concentration. Thus, the research results can provide a theoretical basis for the development and utilization of polysaccharides from C. squamulosa.

Structural characteristics and biological activities of polysaccharides from barley: a review.

Barley (Hordeum vulgare L.) is rich in starch and non-starch polysaccharides (NSPs), especially β-glucan and arabinoxylan. Genotypes and isolation methods may affect their structural characteristics, properties and biological activities. The structure-activity relationships of NSPs in barley have not been paid much attention. This review summarizes the extraction methods, structural characteristics and physicochemical properties of barley polysaccharides. Moreover, the roles of barley β-glucan and arabinoxylan in the immune system, glucose metabolism, regulation of lipid metabolism and absorption of mineral elements are summarized. This review may help in the development of functional products in barley.

Effects of Hydrolysis Condition and Detection Method on the Monosaccharide Composition Analysis of Polysaccharides from Natural Sources

Monosaccharide composition analysis is essential to the structural characterization and research into the biological activity of polysaccharides. In this study, a systematic comparison was performed among commonly used monosaccharide composition analysis methods, including colorimetric and chromatographic methods. These were tested on 16 aldoses, ketoses, alditols, amino sugars, and uronic acids. Furthermore, the effect of hydrolysis methods was also investigated. The results showed that the phenol sulfuric acid method is greatly affected by the type of monosaccharide that is used as the reference substance. The determination of uronic acid using sulfuric acid carbazole is less affected by neutral sugars than that method using m-hydroxybiphenyl. The high-performance thin-layer chromatography (HPTLC) method can simultaneously analyze multiple samples and accurately determine the type of uronic acid. High-performance liquid chromatography (HPLC) can provide a good qualitative and quantitative analysis of aldose, amino sugars, and uronic acids, while gas chromatography–mass spectrometry (GC-MS) can detect aldose, ketose, and alditols. Fructose was detected in a large amount in inulin and Codonopsis pilosula after one-step hydrolysis, while it was totally destroyed in two-step hydrolysis. The release of galacturonic acid significantly increased after two-step hydrolysis in pectin and Lycium barbarum, which indicated that one-step hydrolysis is not enough for acidic polysaccharides. The results of this study are beneficial for selecting appropriate hydrolysis and analysis methods in order to accurately analyze the monosaccharide compositions of natural polysaccharides.

Changes in the physicochemical characteristics and biological activities of polysaccharides from Panax ginseng during defense against red-skin disease

Red-skin roots are a common disease symptom in cultivation of Panax ginseng, which strongly affects its quality and production. Differences in polysaccharides between red-skin and healthy ginseng were examined for the first time to elucidate their function as resistance to disease and explore their potential as active ingredients. Fluorol Yellow 088 and ruthenium red staining revealed changes in the distribution and composition of ginseng polysaccharides during defense against red-skin disease. Acid pectin content was observed to increase significantly in cell walls of the diseased epidermal tissue. Furthermore, targeted extraction approaches were used to obtain water-solution polysaccharides (WPS) and pectic polysaccharides (PP) which marked different characteristics with the health group. WPS from red-skin roots exhibited novel pectic characteristics, consisting of 32.25% GalA which is 10 times more than the healthy sample, accompanied by a sharp decrease in starch content (approximately 50%). Another study on the adsorption and gel properties of PP indicated that it played an important role in disease resistance by reducing metal toxicity and increasing the strength of the cell wall. For further analysis of the abnormal pectic composition, DEAE-52 purification was used and various pectic fractions (WPS-1,2,3) consisting of the RG-Ⅰ pectic domain were obtained. The analysis of antioxidant and immunoactivity was performed to explore the effect of characteristic changes on biological activities. Higher radical scavenging ratios of DPPH and ABTS indicated that red-skin fractions had stronger antioxidant activities. Stronger promotion effect on Raw264.7 secretion of TNF-α and IL-1α represented the higher immunological activities. Therefore, the red-skin ginseng polysaccharides could be utilized in the pharmaceutical industry to compensate for the value loss caused by red-skin roots.

Fermentation-mediated variations in structure and biological activity of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg

Variations in the structure and activities of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg fermented by Sanghuangporus sanghuang fungi were investigated. Compare with the unfermented polysaccharide (THDP2), the major monosaccharide composition and molecular weight of polysaccharide after fermentation (F-THDP2) altered dramatically, which caused galactose-induced conversion from glucose and one-third of molecular weight. F-THDP2 had a molecular weight of 1.23 × 104 Da. Moreover, the glycosidic linkage of F-THDP2 varied significantly, a 1, 2-linked α-d-Galp and 1, 2-linked α-d-Manp backbone was established in F-THDP2, which differed from that of 1, 4-linked α-d-Glcp and 1, 4-linked β-d-Galp in THDP2. In addition, F-THDP2 showed a more flexible chain conformation than that of THDP2 in aqueous solution. Strikingly, F-THDP2 exhibited superior inhibitory effects on HeLa cells via Fas/FasL-mediated Caspase-3 signaling pathways than that of the original polysaccharide. These variations in both structure and biological activities indicated that fermentation-mediated modification by Sanghuangporus sanghuang might a promising novel method for the effective conversion of starch and other polysaccharides from Tetrastigma hemsleyanum Diels et Gilg into highly bioactive biomacromolecules, which could be developed as a potential technology for use in the food industry.

Effects of different extraction methods on the physicochemical properties and biological activities of polysaccharides from maca roots

ABSTRACT Maca is rich in bioactive compounds and has considerable potential for commercial application as a functional food material. In this study, maca root polysaccharides (MRPs) were prepared via hot water extraction, enzyme-assisted extraction, ultrasound-assisted extraction (UAE), and deep eutectic solvent (DES)-based UAE, and their physicochemical properties and biological activities were compared. The MRP obtained using DES-based UAE (MRP-DU) exhibited the highest yield (30.85%), oil-holding capacity (5.21 g/g), emulsifying capacity (50.58%), and emulsion stability (48.14%). Additionally, MRP-DU exhibited the highest antidiabetic and absorption-retarding effects on glucose and bile acid. These results suggest that MRP obtained using DES-based UAE have high application potential as functional food materials and could also serve as antidiabetic and cholesterol-lowering agents.

Physicochemical characterization and biological activity of polysaccharides from the seeds of the turnip brassica rapa

Analysis of the monosaccharide composition showed that the composition of the neutral polysaccharide BSP-1-1 is represented by monosaccharides in the following composition: ribose – 5.05%, arabinose – 56.38%, mannose – 5.87%, glucose – 8.63% and galactose – 24.05%. The composition of the anionic polysaccharide BSP-2-1 is represented by monosaccharides: ribose – 6.35%, arabinose – 60.15%, mannose – 7.19%, glucose – 4.12% and galactose – 22.16%. It was determined that the isolated polysaccharides consist mainly of arabinose (BSP-1-1 – 56.3%, BSP-2-1 – 60%) and galactose (BSP-1-1 – 24%, BSP-2-1 – 22%). Based on the data obtained, it can be assumed that the studied polysaccharides from the seeds of the turnip Brassica rapa belong to the type of arabinogalactans. According to the results of studying the properties of acute toxicity of the BSP polysaccharide from the seeds of the turnip Brassica rapa, it was found that this substance belongs to class V compounds - practically non-toxic substances. With intragastric administration of inulin at doses of 25, 50, and 75 mg/kg and BSP polysaccharide from the seeds of the turnip Brassica rapa at doses of 10, 20 and 30 mg/kg 14 days after the induction of diabetes mellitus, the indicator was close to that of the intact group of animals (intact - 3.07 ± 0.25, inulin 2.99 - 3.14 ± 0.23, and BSP polysaccharide from the seeds of the turnip Brassica rapa 2.53-3.14 ± 0.20), while the indicator of the control group was - 8.40 ± 0.35. This study will provide an opportunity to plan and conduct studies to study the action of substances in the metabolism of glucose and lipids, which examines the enzymes that regulate the lipid activity of the liver, as well as the morphology of the liver and adipose tissue.

Lentinula edodes Sing Polysaccharide: Extraction, Characterization, Bioactivities, and Emulsifying Applications.

In the present work, the optimization of extraction, emulsifying properties, and biological activities of polysaccharides from Lentinula edodes Sing (LES) were studied. The results showed LES polysaccharides extracted by hot water or ultrasonication are a group of β-glucan. Among all the samples, the one extracted by hot water showed the best emulsifying capacity. In addition, the results demonstrated that LES polysaccharide had strong scavenging activities in vitro on DPPH and ABTS radicals, which reached the highest level for the one extracted by 90 min ultrasonication (p < 0.05). Overall, Lentinula edodes Sing polysaccharides (LESPs) may have potential applications as emulsifying agents in food industries.

Characterization and biological activity of acidic sugarcane leaf polysaccharides by microwave-assisted hot alkali extraction

As a waste of sugarcane, the added value of sugarcane leaves has not been fully utilized, and sugarcane leaf polysaccharide is an important active component of sugarcane leaves. Using new extraction methods to study the structure and biological activity of polysaccharides from sugarcane leaves is of great significance for the development and application of sugarcane leaves. To determine the structural characteristics and biological activities of different acidic sugarcane leaf polysaccharides (ASLPs) extracted by microwave-assisted hot alkali extraction (MALE), three new acidic sugarcane leaf polysaccharides (ASLP-50, ASLP-70 and ASLP-90) were precipitated by ethanol. The structure of ASLPs extracted by MALE contains protein, sulfate and uronic acid. ASLP-50 (195.88 kDa), ASLP-70 (94.62 kDa) and ASLP-90 (16.37 kDa) were acidic polysaccharides composed of mannose, galacturonic acid, glucose, arabinose and fucose. The results of XRD and NMR showed that ASLPs were amorphous polysaccharides composed of α-L-Araf-(1→ main chain, and its branching points were composed of Arap and GalpA residues. In addition, ASLPs showed significant antioxidant, hypoglycemic, hypolipidemic and antibacterial activities in vitro. The research and application of ASLPs provide a reference for the recycling of sugarcane leaves, which is conducive to the development of food, agriculture and pharmaceutical industries.