Biogenic Amines Histamine Research Articles

Development of a new high performance liquid chromatography-fluorescence detection (HPLC-FL) method for the determination of biogenic amines in frozen fish sold in markets

<p class="MsoNormal" style="text-align:justify;line-height:200%"><span lang="EN-GB" style="font-size:12.0pt;line-height:200%;font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language: ZH-CN">It is now possible to measure the biogenic amines histamine, tryptamine, putrescine, cadaverine, spermidine, spermine, and tyramine in an easy, accurate, and selective manner. Dansyl chloride was used to purify the analytes, and high-performance liquid chromatography with detection of fluorescence was utilized for separating them. This is a useful method for identifying and measuring biogenic amines in fish samples in a selective manner. The C18 (250 mm × 4.6 mm × 5 μm) column was used for the separation by chromatography. A mobile phase comprising acetonitrile and phosphate buffer (pH=4.0) (60:40, v/v) was used at an average flow rate of 0.9 mL/min. The technique relied on utilizing fluorescence detection to determine the derivative </span><span style="font-size:12.0pt;line-height:200%;font-family: "Times New Roman","serif"">(λ<sub>ex</sub>=230 nm, λ<sub>em</sub>= 350 nm) </span><span lang="EN-GB" style="font-size:12.0pt;line-height:200%;font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-GB;mso-fareast-language: ZH-CN">The suggested method's linearity was examined between 0.01 and 50 μg/mL </span><span style="font-size:12.0pt;line-height:200%;font-family:"Times New Roman","serif"">(r<sup>2</sup>=0.9996). </span><span lang="EN-GB" style="font-size:12.0pt;line-height:200%;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-ansi-language: EN-GB;mso-fareast-language:ZH-CN">In compliance with ICH criteria, the method was verified with respect to accuracy, precision, repeatability, specifity, robustness, and detection and quantification limitations. The suggested technique has been effective in tracking the production of biogenic amines in specimens of fish that can be purchased frozen in marketplaces. It was discovered that the recommended approach worked well for routinely analyzing fish sample biogenic amine analyses.<o:p></o:p></span></p>

Microbiological profile and prevalence of histamine-producing bacteria in fresh sardines stored at different temperatures

Background and Aim: The European pilchard (Sardina pilchardus) is an important fish species for the Moroccan economy in terms of production and export. Biogenic amine histamine is a metabolite produced in the flesh of some fish species after death due to the decarboxylation of free histidine by histaminogenic bacteria. Failure to control the histamine risk in European pilchard may lead to public health and socioeconomic issues. This study aimed to estimate the prevalence of histaminogenic bacteria in association with histamine levels and the growth of microflora in Moroccan sardines (European pilchard). Materials and Methods: We conducted the study by monitoring Moroccan sardines of histamine content and microbiological profile (aerobic plate count [APC], coliforms, and thermo-tolerant coliforms [TTC]) during 6 days of storage at three different temperatures (0°C, 10°C, and ambient temperature [22°C]). The histamine assay was performed using a spectrofluorometric method, and the microbiological identification of histamine-producing bacteria was performed using a combination of biochemical and molecular tests. Results: The histamine content in European pilchard stored at 0°C was negligible. However, high concentrations were observed at 10°C and 22°C. The microbiological profile showed a positive association between microflora counts and histamine content according to storage time. At 0°C, a moderate increase in the APC, a decrease in coliforms, and an absence of TTC were observed. The rapid proliferation of all microflora was observed at 10°C, whereas at 22°C, the proliferation was almost exponential. Bacterial identification revealed the exclusive presence of species belonging to the Enterobacteriaceae family at varying frequencies depending on storage temperature. Morganella morganii and Proteus mirabilis had the highest histamine induction rates in L-histidine-supplemented broth, with 1600 and 255 parts per million (ppm), respectively, after 48-h incubation at 35°C. Klebsiella ozaenae could produce 136 ppm and Serratia plymuthica 115 ppm. Reverse transcription polymerase chain reaction showed positive results for the presence of genes associated with histidine decarboxylase. The hdc genes of M. morganii, P. mirabilis, and K. ozaenae were successfully amplified and exhibited strong similarity with the reference gene of M. morganii. Conclusion: This study describes for the first time the hdc gene in bacteria that form histamine in Moroccan sardines. The results also confirm that respect for the cold chain integrity is a crucial factor in histamine management. This information should help stakeholders in the implementation of sound strategies for managing the hazards associated with seafood and their products. Keywords: Enterobacteriaceae, histamine, Histaminogenic bacteria, polymerase chain reaction, sardine, seafood.

Genome mining and physiological analyses uncover adaptation strategies and biotechnological potential of Virgibacillus dokdonensis T4.6 isolated from high-salt shrimp paste.

Virgibacillus spp. stand out as a potent starter culture for accelerating the fermention of fish sauces and shrimp pastes. However, the underlying molecular mechanisms responsible for their adaptation and biotechnological potential remain elusive. Therefore, the present study focuses on phenotypic and genomic analyses of a halophilic bacterium Virgibacillus dokdonensis T4.6, derived from Vietnamese high-salt fermented shrimp paste. The draft genome contained 4,096,868bp with 3780 predicted coding sequences. Genome mining revealed the presence of 143 genes involved in osmotic adaptation explaining its resistant phenotype to 24% (w/v) NaCl. Among them, 37 genes making up the complete ectoine metabolism pathway, confirmed its ability to produce 4.38 ± 0.29 wt% ectoine under 12.5% NaCl stress. A significant finding was the identification of 39 genes responsible for an entire degradation pathway of the toxic biogenic amine histamine, which was in agreement with its histamine degradation rate of 42.7 ± 2.1% in the HA medium containing 5 mM histamine within 10 days at 37°C. Furthermore, 114 proteolytic and 19 lipolytic genes were detected which might contribute to its survival as well as the nutrient quality and flavor of shrimp paste. Of note, a putative gene vdo2592 was found as a possible novel lipase/esterase due to its unique Glycine-Aspartate-Serine-Leucine (GDSL) sequence motif. This is the first report to reveal the adaptative strategies and related biotechnological potential of Virgibacillus associated with femented foods. Our findings indicated that V. dokdonensis T4.6 is a promising starter culture for the production of fermented shrimp paste products.

The antiglycation potential of H1 receptor antagonists – in vitro studies in bovine serum albumin model and in silico molecular docking analyses

The H1 receptor belongs to the family of rhodopsin-like G-protein-coupled receptors activated by the biogenic amine histamine. H1 receptor antagonists are widely used in the treatment of allergies. However, these drugs could have a much broader spectrum of activity, including hypoglycemic effects, which can broaden the spectrum of their use. The aim of the study was to evaluate the antiglycation potential of twelve H1 receptor antagonists (diphenhydramine, antazoline, promethazine, ketotifen, clemastine, pheniramine, cetirizine, levocetirizine, bilastine, fexofenadine, desloratadine, and loratadine). Bovine serum albumin (BSA) was glycated with sugars (glucose, fructose, galactose, and ribose) and aldehydes (glyoxal and methylglyoxal) in the presence of H1 blockers. The tested substances did not induce a significant decrease in the content of albumin glycation end-products, and the inhibition rate of glycoxidation was not influenced by the chemical structure or generation of H1 blockers. None of the tested H1 receptor antagonists exhibited strong antiglycation activity. Antiglycemic potential of H1 blockers could be attributed to their antioxidant and anti-inflammatory activity, as well as their effects on carbohydrate metabolism/metabolic balance at the systemic level.

Bergamot by-product, a source of biogenic amines, reduces hypertension induced by high sugar-fat diet

The high-sugar fat diet (HSF) generates hypertension. Bergamot by-product (BBP) is a source of hydrophilic bioactive compounds. The aim was to test BBP administration against hypertension induced by the HSF. The 48 male Wistar rats were equally divided into 4 groups: control diet (C), C+BBP, HSF, and HSF+BBP. The BBP (250 mg/Kg) was administrated by gavage for 20 weeks. The total phenolic compounds, carotenoids, and antioxidant capacity of BBP were evaluated by spectrophotometry, and HPLC was used to evaluate the biogenic amines (BAs) profile. The animals' body weight (BW), caloric intake, glucose levels, superoxide dismutase activity, and systolic blood pressure (SBP) were measured at the final of the experiment. Results showed that BBP is a good source of bioactive compounds, such as phenolic compounds (15 ± 2.1 µg Gallic Acid Equivalents/mg, w-w) and flavonoids (5.09 ± 0.22 µg Quercetin Equivalents/mg, w-w), and has a high antioxidant capacity (5.35 ± 0.06 µg Trolox Equivalents (TE)/mg, w-w; 1.30 ± 0.14 µg TE/mg, and 2.54 ± 0.31 mM FeSO/mg), by three different methods). BBP contains the BA histamine (28.4 ± 3.1 µg/g, w-w), tyramine (6.39 ± 0.39 µg/g, w-w), tryptamine (186 ± 11 µg/g, w-w), putrescine (29.0 ± 4.4 µg/g, w-w), cadaverine (10.6 ± 0.8 µg/g, w-w), spermidine (34.8 ± 4.3 µg/g, w-w), spermine (2.28 ± 0.25 µg/g, w-w), serotonin (4.46 ± 0.77 µg/g, w-w), dopamine (2.50 ± 0.29 µg/g, w-w), and agmatine (140 ± 10 µg/g, w-w). BBP has remarkable levels of antioxidant BAs, such as spermidine (34.8 ± 4.3 µg/g, w-w) and agmatine (140 ± 10 µg/g, w-w). HSF increased BW (532 ± 72 g VS 474 ± 36 g (C), p<0.001), glucose levels (91.4 ± 6.2 mg/dL VS 80.9 ± 7.1 mg/dL (C), p<0.001) and SBP (146 ± 9 mmHg VS 121 ± 5 mmHg (C), p<0.001). BBP reduced hypertension generated by HSF (133 ± 9 mmHg VS 146 ± 9 mmHg, p<0.001), suggesting a direct hypotensive action. The synergy between the bioactive compounds identified in BBP demonstrates great potential for use to prevent and treat hypertension.

Highly selective and sensitive histamine and tryptamine analysis using SiO2@AuNPs@PDA molecularly imprinted polymer coupled with SALDI–TOF MS

A combination of SiO2@AuNPs@PDA molecularly imprinted and surface-assisted laser desorption/ionization–time-of-flight mass spectrometry (SALDI–TOF MS) was devised as a method for highly specific and ultrasensitive detection of two biogenic amines—histamine (HIS) and tryptamine (TRP)—in real samples. In this strategy, AuNPs modified amino-abundant silica nanospheres (SiO2@AuNPs). The prepared SiO2@AuNPs were used as a substrate to synthesize a molecularly imprinted polymer (MIP) through in situ dopamine self-polymerization with HIS and TRP as the template molecules (SiO2@AuNP@PDA-MIP). The as-prepared MIP structure, properties, and target-analyte identification conditions were characterized and optimized and it was used as the matrix for MS. Compared to the case of nonimprinted materials, the imprinting function endowed the matrix with a higher selectivity for capturing the target molecules. The enriched analytes were directly and rapidly identified using SALDI–TOF MS without elution. Meanwhile, the proposed method has low background interference, good reproducibility and stability, high salt tolerance, and satisfactory linearity (R2 > 0.99), and it enables ultrasensitive detection of HIS and TRP (limits of detection for HIS and TRP were 0.2 and 0.1 ng mL−1, respectively). Moreover, the proposed method was applied to analyze samples of real beer, sausage, and chicken, and the results agreed with those obtained via liquid chromatography–MS, suggesting that the method has excellent practical applications in the field of food safety.

A neurotransmitter histamine mediating phototransduction and photopreference in Callosobruchus maculatus.

The biogenic amine histamine plays a critical role in the phototransduction and photopreference of most insects. Here, we study the function of histamine in Callosobruchus maculatus, a global storage pest. In our experiment, we initially identified the histidine decarboxylase (hdc) gene through bioinformation analysis. We subsequently investigated effects of hdc and histamine on the photopreference of C. maculatus using a combination of RNA interference (RNAi), electroretinograms (ERG), immunostaining, and photopreference behavior approaches. Our results showed that histamine was required for visual signal transduction of C. maculatus, and increased its photopreference regardless of the wavelength. This is the first study analyzing the molecular characteristics of C. maculatus photopreference, which forms the basis for a molecular mechanism for the effects of histamine on its visual transduction and preference. In practice, better understanding the photopreference patterns contributes to IPM (integrated pest management) for this storage pest. © 2023 Society of Chemical Industry.

Pentafluoropropionic Anhydride Derivatization and GC-MS Analysis of Histamine, Agmatine, Putrescine, and Spermidine: Effects of Solvents and Starting Column Temperature.

Gas chromatography-mass spectrometry (GC-MS) is useful for the quantitative determination of the polyamines spermidine (SPD) and putrescine (PUT) and of the biogenic amine agmatine (AGM) in biological samples after derivatization. This GC-MS method involves a two-step extraction with n-butanol and hydrochloric acid, derivatization with pentafluoropropionic anhydride (PFPA) in ethyl acetate, and extraction of the pentafluoropropionic (PFP) derivatives by toluene of SPD, PUT, and AGM. We wanted to extend this GC-MS method for the biogenic amine histamine (HA), but we faced serious problems that did not allow reliable quantitative analysis of HA. In the present work, we addressed this issue and investigated the derivatization of HA and the effects of toluene and ethyl acetate, two commonly used water-insoluble organic solvents in GC-MS, and oven temperature program. Derivatization of unlabelled HA (d0-HA) and deuterium-labelled HA (d4-HA) with PFPA in ethyl acetate (PFPA-EA, 1:4, v/v; 30 min, 65 °C) resulted in the formation of d0-HA-(PFP)2 and d4-HA-(PFP)2 derivatives. d4-HA and 13C4-SPD were used as internal standards for the amines after standardization. Considerable quantitative effects of toluene and ethyl acetate were observed. The starting GC column temperature was also found to influence considerably the GC-MS analysis of HA. Our study shows the simultaneous quantitative analysis of HA as HA-(PFP)2, AGM as AGM-(PFP)3, PUT as PUT-(PFP)2, and SPD as SPD-(PFP)3 derivatives requires the use of ethyl acetate for their extraction and injection into the GC-MS apparatus and a starting GC column temperature of 40 °C instead of 70 °C. The PFP derivatives of HA, AGM, PUT, and SPD were found to be stable in ethyl acetate for several hours at room temperature. Analytically satisfactory linearity, precision, and accuracy were observed for HA, AGM, PUT, and SPD in biologically relevant ranges (0 to 700 pmol). The limits of detection of AGM, PUT, and SPD were about two times lower in ethyl acetate compared to toluene (range, 1-22 fmol). The limits of detection were 1670 fmol for d0-HA and 557 fmol for d4-HA. Despite the improvements achieved in the study for HA, its analysis by GC-MS as a PFP derivative is challenging and less efficient than that of PUT, AGM, and SPD.

Repurposing Histaminergic Drugs in Multiple Sclerosis.

Multiple sclerosis is an autoimmune disease with a strong neuroinflammatory component that contributes to severe demyelination, neurodegeneration and lesions formation in white and grey matter of the spinal cord and brain. Increasing attention is being paid to the signaling of the biogenic amine histamine in the context of several pathological conditions. In multiple sclerosis, histamine regulates the differentiation of oligodendrocyte precursors, reduces demyelination, and improves the remyelination process. However, the concomitant activation of histamine H1–H4 receptors can sustain either damaging or favorable effects, depending on the specifically activated receptor subtype/s, the timing of receptor engagement, and the central versus peripheral target district. Conventional drug development has failed so far to identify curative drugs for multiple sclerosis, thus causing a severe delay in therapeutic options available to patients. In this perspective, drug repurposing offers an exciting and complementary alternative for rapidly approving some medicines already approved for other indications. In the present work, we have adopted a new network-medicine-based algorithm for drug repurposing called SAveRUNNER, for quantifying the interplay between multiple sclerosis-associated genes and drug targets in the human interactome. We have identified new histamine drug-disease associations and predicted off-label novel use of the histaminergic drugs amodiaquine, rupatadine, and diphenhydramine among others, for multiple sclerosis. Our work suggests that selected histamine-related molecules might get to the root causes of multiple sclerosis and emerge as new potential therapeutic strategies for the disease.

Determination of biogenic amines formation by autochthonous lactic acid bacteria from ‘Refošk’ grapes using different analytical methods

Different analytical methods were tested and optimized for the determination of four biogenic amines (BA) histamine, putrescine, cadaverine and tyramine produced by grape-associated lactic acid bacteria (LAB). Autochthonous LAB were isolated from ‘Refošk’ grapes belonging to Slovenian-Italian Karst region as they represent a potential pool of beneficial LAB starter bacteria for improving the typicality and quality of wine. Six isolated strains were screened by multiplex PCR, of which four strains were positive for BA-forming genes (MKBT-49, MKBT-282, MKBT-568, MKBT-570). The production of BA was evaluated by thin-layer chromatography (TLC), high-performance liquid chromatography coupled to diode array detection (HPLC-DAD) and enzymatic method. The HPLC-DAD results showed that strain MKBT-49 (tyrdc+) had high tyramine production (386.6 ± 0.14 mg/L), which was confirmed by TLC. The ability to produce putrescine was confirmed in strain MKBT-282 by PCR, HPLC-DAD (16.4 ± 1.72 mg/L), and TLC. Histamine-producing ability was detected in strain MKBT-570, with a concentration below the limit of detection of the HPLC-DAD (<0.2 mg/L), while the other two methods were not sensitive enough for confirmation. This study shows that the production of BA can detected in native LAB and that relatively simple method such as TLC can be used effectively for the initial screening.

Specific determination of histamine in cheese and cured meat products by ion chromatography coupled to fluorimetric detection

A simple, specific and reliable method for the determination of the biogenic amine histamine in cheese and cured met products has been optimized and validated. The proposed analytical scheme is based on the powerful combination of cation exchange chromatography to sensitive and selective post column derivatization. The fluorescence post column reaction between histamine and o-phthalaldehyde was systematically optimized using Box-Behnken experimental design, while the method was validated in terms of linearity, LOD, precision, matrix effect and accuracy. The ruggedness of the method was investigated using Monte-Carlo simulations. The LOD of 0.15 mg kg−1 was quite satisfactory for food analysis, while the absence of matrix effect (< ± 5.5%) enabled quantification based on the aqueous calibration curve, also avoiding complicated pretreatment procedures. Various cheese and cured meat products were analyzed successfully with percent recoveries in the range of 91.3 – 116.9%.

PHYSICOCHEMICAL AND MICROBIOLOGICAL PROPERTIES OF SALTED FERMENTED FISH (FESEEKH) CONSUMED IN EGYPT

The salted fermented fish (Mugil cephlus) Feseekh is not only popular as an appetizer; but also it's popular in some festivities in Egypt as a main dish. Biogenic amines (BA) are known as toxic in food. This study was conducted to investigate biogenic amines (BAs), physicochemical properties, and some microorganisms in salted fermented fish (Feseekh). Fifteen samples of Feseekh samples were obtained from different Egyptian local markets in four governorates (Sharkia, Ismailia, Beheira, and Dakahlia). The total biogenic amine content in Feseekh varied between 104.6 and 166.8 mg/kg. Six BAs Histamine (HIS), Tyramine (TYR), Putrescine (PUT), Cadaverine (CAD), Spermine (SPE), and Spermidine (SPD) were found in all fish samples under investigation. Histamine was quantitatively the most common biogenic amine in all samples, the histamine content ranged from 51.80 to 93.3 mg/kg, which was above the 50 mg/kg limits set by the Food and Drug Administration (FDA). Fermented fish (Feseekh) contains 14 amino acids. The essential amino acids accounted for 30.7-36.9% of the total concentration of amino acids. Fish flesh of mullet had the highest fatty acids that are saturated fatty was less than monounsaturated and polyunsaturated fatty acids. The total volatile basic-nitrogen (TVBN) content was ranged from 23.00 to 48.00 mgN/100g, Thiobarbituric acid (TBA) content was from 1.36 to 2.94 mg malonaldehyde/kg sample and Trimethylamine (TMA) was from 3.20 to 23.10 mgN/100g. Meanwhile, The total bacteria count(TBC) was from (6.48 to 7.27 log CFU/g) was significantly higher than that of total mesophilic bacteria count (TMC) ranged from 6.04 to 7 log CFU/g. It could be inferred that the safety of Feseekh should be improved by hygienic manufacturing process.

A landscape for drug-target interactions based on network analysis.

In this work, we performed an analysis of the networks of interactions between drugs and their targets to assess how connected the compounds are. For our purpose, the interactions were downloaded from the DrugBank database, and we considered all drugs approved by the FDA. Based on topological analysis of this interaction network, we obtained information on degree, clustering coefficient, connected components, and centrality of these interactions. We identified that this drug-target interaction network cannot be divided into two disjoint and independent sets, i.e., it is not bipartite. In addition, the connectivity or associations between every pair of nodes identified that the drug-target network is constituted of 165 connected components, where one giant component contains 4376 interactions that represent 89.99% of all the elements. In this regard, the histamine H1 receptor, which belongs to the family of rhodopsin-like G-protein-coupled receptors and is activated by the biogenic amine histamine, was found to be the most important node in the centrality of input-degrees. In the case of centrality of output-degrees, fostamatinib was found to be the most important node, as this drug interacts with 300 different targets, including arachidonate 5-lipoxygenase or ALOX5, expressed on cells primarily involved in regulation of immune responses. The top 10 hubs interacted with 33% of the target genes. Fostamatinib stands out because it is used for the treatment of chronic immune thrombocytopenia in adults. Finally, 187 highly connected sets of nodes, structured in communities, were also identified. Indeed, the largest communities have more than 400 elements and are related to metabolic diseases, psychiatric disorders and cancer. Our results demonstrate the possibilities to explore these compounds and their targets to improve drug repositioning and contend against emergent diseases.

Biogenic Amines as Freshness Indicator in Halal and Non-Halal Slaughtered Chicken Meat Using Chromatographic Approach

Biogenic amines are naturally occurring organic bases produced by bacterial decarboxylation of amino acids that have been associated with harmful toxicology effects to humans. The main objective of this work is to investigate the possible role of biogenic amines (BAs) as indicators of spoilage in fresh chicken meat stored at 4°C for 15 days. A reversed-phase high-performance liquid chromatographic method with isocratic elution system is used for the quantification of four biogenic amines (putrescine, histamine, tyramine, cadaverine and spermine as well as spermidine) in chicken meat. Amines were extracted with 5% of trichloroacetic acid (TCA) and derivatised using dansyl chloride. The variation storage time differentiated the chicken meat on the microbiological characteristics. The results obtained shows that histamine, spermidine, tyramine, cadaverine, and putrescine increased slowly while spermine decreased for both chicken breast in halal and non-halal chicken meat. Thus, this BAs could be used as a spoilage index of fresh chicken meat.

Histamine-4 Receptor: Emerging Target for the Treatment of Neurological Diseases.

A major challenge in the field of the biogenic amine histamine is the search for new-generation histamine receptor specific drugs. Daniel Bovet and Sir James Black received their Nobel Prizes for Medicine for their work on histamine-1 receptor (H1R) and H2R antagonists to treat allergies and gastrointestinal disorders. The first H3R-targeting drug to reach the market was approved for the treatment of the neurological disorder narcolepsy in 2018. The antagonists for the most recently identified histamine receptor, H4R, are currently under clinical evaluation for their potential therapeutic effects on inflammatory diseases such as atopic dermatitis and pruritus. In this chapter, we propose that H4R antagonists are endowed with prominent anti-inflammatory and immune effects, including in the brain. To substantiate this proposition, we combine data from transcriptional analyses of postmortem human neurodegenerative disease brain samples, human genome-wide association studies (GWAS), and translational animal model studies. The results prompt us to suggest the potential involvement of the H4R in various neurodegenerative diseases and how manipulating the H4R may create new therapeutic opportunities in central nervous system diseases.

Detection of Biogenic Amines in Indian Fish and Fishery Products Consumed in Southeast Coast Region of India

ABSTRACT The aim of the present study was to assess the occurrence of seven biogenic amines (cadaverine, histamine, putrescine, tryptamine, tyramine, spermidine, and spermine) in 13 fresh fish, three crustaceans, and three cephalopod species, as well as six cured and dried fish products commonly consumed in the southeast coast region of India. The biogenic amines were analyzed by high performance liquid chromatography with a dansyl chloride derivatization. In the majority of the cases, the biogenic amine content was within the limits set at both national and international levels. The only exceptions were the histamine content of fresh Silago sihama, Lutjanus bohar, and L. johnii, cured and dried Rastrelliger kanagurta and the tyramine content of cured and dried Penaeus indicus. In these cases, lack of proper temperature handling was indicated, which highlights the importance of establishing and implementing proper handling procedures to ensure fish and fishery product safety for human consumption.

Addition of Coriander during Fermentation of Korean Soy Sauce (Gangjang) Causes Significant Shift in Microbial Composition and Reduction in Biogenic Amine Levels.

The microflora of Korean soy sauce (gangjang) play an important role in maintaining its quality and safety. Hence, it is important to study the microflora and the possible approaches to improve their composition. In this study, the effect of adding coriander during soy sauce fermentation on the microflora and biogenic amines was evaluated using metagenomics and 1H NMR analyses, respectively. The β-diversity showed a clear distinction between the microbiota of the coriander and control groups. Microbial composition analysis revealed noticeable shifts, as Firmicutes abundance was significantly higher in the coriander group (91.77%) than that in the control (38.78%). The dominant bacterial family in the coriander group was the Bacillaceae (57.94%), while Halomonadaceae was dominant in the control group (49.77%). At the species level, Chromohalobacter beijerinckii dominated the microbial community in the control group (49.54%), but not (4.43%) in the coriander group. Moreover, there was a negative correlation between the Bacillaceae and several other bacterial families, including Halomonadaceae, which indicated a possible antagonism and partly explained the reduction in Chromohalobacter abundance in the coriander group. The levels of the biogenic amines histamine, putrescine, and tyramine, which are considered potential health risk factors, were significantly lower in the coriander soy sauce than those in the control sauce. The results of this study suggest that the addition of coriander during Korean soy sauce fermentation is beneficial, as coriander significantly reduces the levels of biogenic amines and the bacteria that produce them.

Biogén amin (hisztamin) intolerancia, társuló tünetek, komorbiditások a bőrgyógyászati-allergológiai gyakorlatban

Biogén amin (hisztamin) intolerancia, társuló tünetek, komorbiditások a bőrgyógyászati-allergológiai gyakorlatban

Determination of Biogenic amines in hard cheeses by High performance liquid chromatography

This study evaluated chemically the quantity of six biogenic amines histamine, tyramine, puterscine, cadaverine, tryptamine and phenylethylamine in forty hard cheese samples (Chedder and Ras) (20 of each) sold in retail markets in Zagazig City Sharkia Governorate using high performance liquid chromatography (HPLC) and correlation with compositional quantity of cheese (pH, salt%, moisture% and acidity%). Our results showed the mean values of pH, salt, moisture and acidity were (5.5±0.02 and 4.79±0.11), (1.53±0.04 and 4.42±0.05%), (40.45±0.24 and 38.64±0.34%) and (0.84 ±0.01 and 1.70±0.04%) in Chedder and Ras cheese samples, respectively. The mean values of total biogenic amines were 12.65±2.7and 28.95±4.6 mg/100g in Chedder and Ras cheese samples, respectively. There was a negative correlation between (pH value and moisture %) and biogenic amines production but the positive correlation was found between (salt and acidity %) and biogenic amines production. Finally it was found that all Chedder and Ras cheese samples were accepted according to maximum permissible limit of Egyptian organization standard (EOS, 2007) which is 20mg/100g. All samples were accepted according to maximum permissible limit of Food and Drug Administration (FDA, 2001) which is 10mg/100g except 60 and 90 % of Ras cheese samples are accepted for histamine and puterscine, respectively. On conclusion, optimization and standardization of milk quality and hygiene during cheeses manufacturing and storage should be taken for human safety.

How Tropospheric Ozone Influences the Allelopathy of Woody Species: Some Experimental Approaches

Plants undergo a high concentration of tropospheric ozone formed mainly as a result of industrial and automobile pollution that may act on allelopathic relations in biocenosis. The problem was considered in first experiments modeling in test reactions with leaf leachates from 10 woody species as plant-donors, exposed to ozone, and able to influence on herbs (plant-acceptors) grown under their canopy. The effects were dependent on the duration and the intensity exposure to ozone. The color and the autofluorescence of the woody plant leaves with secretory cells contained allelochemicals changed under the ozone treatment. The effects of water extracts (models of rain leachates) from leaves of woody species exposed to ozone on the seed germination of herb Lavatera trimestris (Malvaceae), plant-acceptor of allelochemicals, differed from untreated samples. This showed a possible transformation of allelochemicals or/and the formation of new similar exometabolites after the ozone treatment. In the fluorescence spectra of whole leaves, the maxima, peculiar to phenols, were found at different experiments, while peaks related to terpenes disappeared in ozonated samples. Under acute or chronic ozone exposure the formation of biogenic amines (dopamine and histamine) known as allelochemicals was observed in leaf cells. These test-reactions on tropospheric ozone stress could be used in the analysis of allelopathic relations in urban conditions.