Source Of Bioenergy Research Articles

Combustion of Pelletized Coffee Residues for Bioenergy Valorization Within a Circular Economy Vision

Combustion of Pelletized Coffee Residues for Bioenergy Valorization Within a Circular Economy Vision

The significance of biowaste drying analysis as a key pre-treatment for transforming it into a sustainable biomass feedstock.

The objective of this study is to investigate the drying kinetics of fruit and vegetable peel biowaste using a sustainable technique as a key-pretreatment for its conversion into useful feedstock. Biowaste represents a missed potential source of bioenergy and bioproducts, but moisture removal is required, and conventional drying methods are expensive since they require great quantity of energy supplied, almost always, by a non-renewable energy. In this study six batches with the same quantity of biowaste, and heterogeneous physical composition were dried under open-sun conditions. We evaluated the influence of the interaction between drying area and the initial moisture content on drying rate. Eight semi-theoretical models were fitted using Levenberg-Marquardt algorithm to predict drying rate, and their accuracy was assessed through goodness-of-fit tests. Maximum moisture content to preserve biomass (10%) was reached on 5th day and the equilibrium on 16th day of drying. According to goodness-of-fit test (R 2=0.999, χ 2=4.666×10-5, RMSE = 0.00683) the best model to predict drying rate was Two-term model. The mathematical model obtained from Fick's second law is reliable to predict drying kinetics, R2 (0.9648±0.0106); despite the variation between drying area and initial moisture content. Kruskal-Wallis test showed that drying rates between batches are not significantly different (p=0.639; 0.05); nor effective diffusion coefficient (D eff =4.97×10-11±0.3491×10-11), (p=0.723; 0.05). The study of drying kinetics is crucial for selecting the optimal biowaste treatment based on its generation context. This could enable its use as feedstock for bioproduct or bioenergy production, thereby reducing waste accumulation in landfills and environmental impact.

Maximizing microbial activity and synergistic interaction to boost biofuel production from lignocellulosic biomass.

Addressing global environmental challenges and meeting the escalating energy demands stand as two pivotal issues in the current landscape. Lignocellulosic biomass emerges as a promising renewable bio-energy source capable of fulfilling the world's energy requirements on a large scale. One of the most important steps in lowering reliance on fossil fuel and lessening environmental effect is turning lignocellulosic biomass into biofuel. As carbon-neutral substitutes for traditional fuel, biofuel offer a solution to environmental concerns compared to conventional fuel. Effective utilization of lignocellulosic biomass is imperative for sustainable development. Ongoing research focuses on exploring the potential of various microorganisms and their co-interactions to synthesize diverse biofuels from different starting materials, including lignocellulosic biomass. Co-culture techniques demonstrate resilience to nutrient scarcity and environmental fluctuations. By utilising a variety of carbon sources, microbes can enhance their adaptability to environmental stressors and potentially increase productivity through their symbiotic interactions. Furthermore, compared to single organism involvement, co-interactions allow faster execution of multistep processes. Lignocellulosic biomass serves as a primary substrate for pre-treatment, fermentation, and enzymatic hydrolysis processes. This review primarily delves into the pretreatment, enzymatic hydrolysis process and the biochemical pathways involved in converting lignocellulosic biomass into bioenergy.

Maize Abiotic Stress Treatments in Controlled Environments.

Maize (Zea mays) is one of the world's most important crops, providing food for humans and livestock and serving as a bioenergy source. Climate change and the resulting abiotic stressors in the field reduce crop yields, threatening food security and the global economy. Water deficit (i.e., drought), heat, and insufficient nutrients (e.g., nitrogen and phosphorus) are major environmental stressors that affect maize yields, and impact growth and development at all stages of the plant life cycle. Understanding the biological processes underlying these responses in maize has the potential to increase yields in the face of abiotic stress. Optimizing individual or combined abiotic stress treatments in controlled environments reduces potential noise in data collection that can be present under less controlled growth conditions. Here, we describe methods and conditions for controlled abiotic stress treatments and associated controls during early vegetative growth of maize, conducted in greenhouses or growth chambers. This includes the environmental conditions, equipment, soil preparation, and intensity and duration of heat, drought, nitrogen deficiency, and phosphorous deficiency. Controlled experiments at early growth stages are informative for future in-field studies that require greater labor and inputs, saving researchers time and growing space, and thus research funds, before testing plants across later stages of development. We suggest that stress treatments be severe enough to result in a measurable phenotype, but not so severe that all plants die prior to sample collection. This protocol is designed to set important standards for replicable research in maize.

Carbohydrate Derived Value-added Products from Lignocelluloses

Abstract: Chemistry is confronted with the pressing issues of depleting non-renewable fossil resources and the imperative to combat environmental pollution, which is crucial for a sustainable future. Biomass stands out as the sole organic carbon source in nature among the array of sustainable resources available, positioning it as a prime substitute for fossil-derived chemicals and fuels. Extensive research has been conducted on the abundant lignocelluloses as a potential source for biofuels, bioenergy, and various valuable products, wherein, the incorporation of various processes in biomass fractionation to separate biopolymers (such as lignin, cellulose, and hemicellulose) has the potential to enhance the overall value of the process. However, industrial demonstration of biomass utilization for commercial products has been limited due to the challenges posed by the recalcitrance and complexity of biomass. Therefore, there is a need for efficient reaction processes to enable the production of bio-chemicals and fuels from renewable lignocellulose. This review focuses on the latest chemical methods developed for producing value-added chemicals from biomass-derived cellulose as a renewable feedstock.

Exploring and evaluating the relationship between Saccharomyces cerevisiae biofilm maturation on carbon felt anodes and microbial fuel cell performance

Microbial fuel cells (MFCs) hold great promise as sustainable bioenergy sources, with their performance intricately linked to the formation and characteristics of biofilms. This study delves into the bio-electrochemical perspective of biofilms in MFCs, aiming to elucidate their pivotal role in MFC functionality. The investigation focused on a yeast-based MFC operated through 48 h per cycle, with cycle 5 marking the maturation stage of biofilm formation. During this phase, voltage stability was observed, with a stationary phase voltage of 38.9±2.6 mV. Notably, cycle 5 exhibited a significant boost in power density, reaching 8.82 mW m-2, accom­panied by the lowest internal resistance of 100 Ω. Furthermore, the electron transfer rate constant from cycle 5 is 1.14±0.02 s-1, 57 times higher than the initial, underscoring biofilm's catalytic potential. Additionally, cyclic voltammetry unveiled non-linear relationships between redox reaction peak current and scan rate, with a consistent DEp of ~219 mV at 100 mV s-1. Importantly, elemental analysis disclosed incorporating diverse elements (Na, Al, Si, P, S, Cl, K, Ca, Cr, and Fe) into the carbon felt, signifying their association with biofilm development. These findings offer critical insights into optimizing MFC performance through biofilm modulation, advancing sustainable bioenergy technologies.

A comprehensive review of biofuel utilization for household cooking in developing countries: Economic and environmental impacts

The global energy consumption for household cooking is substantial, comprising approximately 90 % of total household energy usage worldwide. Research indicates that in 2002, food-related energy consumption averaged 340 million BTU (approximately 359 million J) per person. With the current global population exceeding 7 billion, this translates to an estimated annual energy consumption for cooking of 2.380×1018 BTU (2.519 ×1021 J). A significant portion of this energy is derived from the combustion of firewood, contributing to deforestation, CO2 emissions, and the release of toxic gases, which in turn, exacerbates environmental pollution and poses health risks. Household cooking, heating, and lighting activities in developing countries account for about 58 % of the black carbon emissions globally. Recognizing clean cooking as a crucial solution to mitigate climate change, there is growing interest in utilizing biomass as a source for biofuels and bioenergy. The comparative diminishing in greenhouse gas emissions from renewable fuels compared to conventional fossil fuels, along with the sustainable nature of these technological advancements, has spurred increased research into biofuel technology. To address energy poverty, achieve the Sustainable Development Goals, and align with the goals of the Paris Agreement, comprehensive and expedited efforts are needed to expand and enhance access to clean cooking solutions. Consequently, this article explores the potential of biofuels as viable alternatives to conventional fossil fuels for household cooking, focusing on their economic and environmental implications.

The impact of shade net use on total nitrogen removal by duckweed (Lemna perpusilla) at different levels of catfish farming effluent

Duckweed (Lemna perpusilla) has been shown to reduce nutrient levels in aquaculture effluent, particularly total nitrogen, which can be caused by a variety of organic contaminants in water. Duckweed biomass is an important source of bioenergy and phytoremediation in aquaculture. This aspect makes it a viable solution for sustainable integrated aquaculture, although appropriate duckweed biomass development is critical to its success and long-term survival. Duckweed growth, as an autotroph, is controlled by two factors: nutrition and sunlight. The aquaculture production cycle changes nutrient content in medium culture based on feed, fish age, and density, while weather influences solar radiation. The study aims to investigate duckweed growth response and phytoremediation capacity by analyzing changes in catfish farming waste-water quality grouped by fish age (L1: 2 months, L2: 3 months, and L3: 4 months) and shade net treatment at 25 % sunlight blocking (N25), 50 % (N50), and no shade net (N0). The experiment was carried out in triplicate in a semi-outdoor growth system placed in a greenhouse. It comprised of three series of duckweed culture containers filled with catfish farming wastewater, each with a working volume of 50 L. A total of 48 duckweed culture containers were floated inside a series of Recirculating Aquaculture System (RAS) tanks. Biomass harvesting and water quality monitoring were done every three days for a period of 18 days. N25 had the highest biomass weight, productivity, and average TN elimination efficiency, with values of 225.21 ± 140.04 g, 50.05 ± 10.06 g/(m2.d), and 61.94 ± 8.01 %, respectively (P < 0.05). The N0 values were 190.80 ± 117.52 g, 42.40 ± 9.29 g/(m2.d), and 55.61 ± 6.85 % (P < 0.05). The lowest values observed in N50 were 72.27 ± 55.70 g, 16.06 ± 4.90 g/(m2.d), and 51.67 ± 4.10 % (P < 0.05). This study proved the optimal duckweed growth and TN removal effectiveness with N25 under varying light intensity, allowing for the long-term use of duckweed in waste management and integrated aquaculture.

Anaerobic co-digestion of cow manure and microalgae to increase biogas production: A sustainable bioenergy source

The biogas production from microalgae has gained attention due to fast depleting of fossil fuels and oil reserves. This study evaluated the anaerobic co-digestion of microalgae in various concentrations with cow manure to enhance biogas production. The biogas production of each experiment was measured using the water displacement method. The results indicated that the addition of microalgae significantly enhanced biogas production. Particularly, high methane yield of Anabaena sp. 50 %, Chlorella sp. 50 %, control was 345 ± 2.88 mL CH4/g VS, 297.96 ± 0.49 mL CH4/g VS, 138.32 ± 0.50 CH4/g VS respectively. The slurry produced by 50 % Anabaena sp. biogas plant exhibited the greatest level of seed germination. The current study demonstrated that Sorgham bicolor had the highest seed germination rate (94.2 %) root and shoot length of all crops. Therefore, it is possible to employ Anabaena sp. (50 %) and Chlorella sp. (50 %) in the rapid production of biogas. Moreover, agricultural output would be increased by using biogas slurry.

Sewage sludge management and enhanced energy recovery using anaerobic digestion: an insight.

Sewage sludge (SS) is a potential source of bioenergy, yet its management is a global concern. Anaerobic digestion (AD) is applied to effectively valorize SS by reclaiming energy in the form of methane. However, the complex floc structure of SS hinders hydrolysis during AD process, thus resulting in lower process efficiency. To overcome the rate-limiting hydrolysis, various pre-treatment methods have been developed to enhance AD efficiency. This review aims to provide insights into recent advancements in pre-treatment technologies, including mechanical, chemical, thermal, and biological methods. Each technology was critically evaluated and compared, and its relative worth was summarized based on full-scale applicability, along with economic benefits, AD performance improvements, and impact on digested sludge. The paper illuminates the readers about existing research gaps, and the future research needed for successful implementation of these approaches at full scale.

Production of Ganodiesel from the biomass of Ganoderma lucidum in air-L-shaped bioreactor (ALSB)

The increasing need for alternative and sustainable energy sources, prompted by the depletion of fossil fuels and the rise in greenhouse gas emissions, has generated attention towards exploring fast-growing filamentous fungi as a potential bioenergy source. This study aimed to optimize Ganoderma lucidum production for elevated biomass and lipid yields in submerged liquid fermentation. The optimization involved varying initial pH, glucose concentration, and agitation rate using response surface methodology (RSM) with central composite design (CCD). Glucose concentration and initial pH significantly influenced biomass production, while agitation rate had an insignificant effect. For lipid production, glucose concentration, initial medium pH, and agitation rate were identified as significant factors. The optimized conditions (initial pH 6, 50 g/L glucose concentration, and 113.42 rpm) were validated in 500 mL shake flasks and a 3 L Air-L-Shaped Bioreactor (ALSB). Shake flask results showed 8.33 g/L of biomass and 2.17 % of lipid, while the ALSB system produced 5.32 g/L of biomass and 2.35 % lipid. The obtained Ganoderma lucidum mycelial lipid underwent acid-catalysed transesterification to produce biodiesel, which was subjected to several tests to comply ASTM and EN standards. This study serves as a valuable reference for future biodiesel applications through the optimization of Ganoderma lucidum biomass and lipid production.

Impact of mustard biodiesel and clove oil additives on particulate matter emission and carbon deposition in diesel engines

Recent studies suggest that the world is facing an energy crisis due to the depletion of fossil fuel reserves. To combat this issue, researchers have turned to biodiesel, a renewable bioenergy source made from vegetable oils, microalgae, and animal fats. A recent study analysed engine parts’ particulate matter emissions and carbon deposition during the long-term use of mustard biodiesel and clove oil as antioxidants in a compression ignition engine. Three samples of fuels: DF (diesel fuel), B30 (30% mustard biodiesel and 70% DF), and biodiesel blended fuel with 3000 PPM in a single-cylinder CI engine. The use of 30% biodiesel in diesel fuel (B30) for the endurance test was based on a good mix. The engine was run for 100 h to investigate the particulate matter emissions and carbon deposition. The particulate matter emission data was collected every 25 h, and for carbon deposition, the engine’s fuel injector was turned off after 100 h of engine running. The results showed a reduction in particulate matter emissions of about 9.97%, 13.367%, 7.24%, 14.64%, 5.3%, 12.32%, 1.88%, and 7.99% for PM1, PM2.5, PM7, and PM10 in biodiesel blended fuel and biodiesel blended fuel with clove oil, respectively. The deposition of clove oil added to biodiesel blended fuel in the fuel injector has been reduced compared with the other fuels. Carbon deposition of the fuel injector was analysed through SEM and EDX tests, and the results showed that the carbon content in biodiesel blended fuel was lower than in diesel fuel. The deposition of clove oil added to biodiesel blended fuel in the fuel injector has been reduced compared with biodiesel blended fuel.

Reviewing the role of bioenergy with carbon capture and storage (BECCS) in climate mitigation

Climate change poses an imminent threat, necessitating innovative and sustainable strategies for mitigation. This paper explores the potential of Bioenergy with Carbon Capture and Storage (BECCS) as a promising approach. The introductory section sets the stage by elucidating the urgency of climate action. The background section surveys existing climate mitigation strategies, introducing bioenergy and carbon capture technologies. The paper delves into the distinctive contributions of bioenergy to carbon emission reduction and assesses the viability of various bioenergy sources. Simultaneously, the discussion on Carbon Capture and Storage (CCS) provides insight into the technological aspects of carbon capture. An integral focus is the integration of bioenergy and carbon capture technologies in BECCS, exploring synergies that enhance their combined efficacy. Real-world examples and case studies illustrate successful BECCS projects. Environmental and social impacts are critically examined, considering sustainability and ethical dimensions. Challenges and criticisms associated with BECCS are discussed comprehensively, addressing concerns and proposing potential solutions. The paper concludes by outlining future prospects for BECCS, offering recommendations for policymakers and stakeholders. It also suggests avenues for further research and development in this evolving field. Keywords: Bioenergy, Carbon Capture and Storage (BECCS), Climate Mitigation.

Assessing global carbon sequestration and bioenergy potential from microalgae cultivation on marginal lands leveraging machine learning

This comprehensive study unveils the vast global potential of microalgae as a sustainable bioenergy source, focusing on the utilization of marginal lands and employing advanced machine learning techniques to predict biomass productivity. By identifying approximately 7.37 million square kilometers of marginal lands suitable for microalgae cultivation, this research uncovers the extensive potential of these underutilized areas, particularly within equatorial and low-latitude regions, for microalgae bioenergy development. This approach mitigates the competition for food resources and conserves freshwater supplies. Utilizing cutting-edge machine learning algorithms based on robust datasets from global microalgae cultivation experiments spanning 1994 to 2017, this study integrates essential environmental variables to map out a detailed projection of potential yields across a variety of landscapes. The analysis further delineates the bioenergy and carbon sequestration potential across two effective cultivation methods: Photobioreactors (PBRs), and Open Ponds, with PBRs showcasing exceptional productivity, with a global average daily biomass productivity of 142.81mgL−1d−1, followed by Open Ponds at 122.57mgL−1d−1. Projections based on optimal PBR conditions suggest an annual yield of 99.54 gigatons of microalgae biomass. This yield can be transformed into 64.70 gigatons of biodiesel, equivalent to 58.68 gigatons of traditional diesel, while sequestering 182.16 gigatons of CO2, equating to approximately 4.5 times the global CO2 emissions projected for 2023. Notably, Australia leads in microalgae biomass production, with an annual output of 16.19 gigatons, followed by significant contributions from Kazakhstan, Sudan, Brazil, the United States, and China, showcasing the diverse global potential for microalgae bioenergy across varying ecological and geographical landscapes. Through this rigorous investigation, the study emphasizes the strategic importance of microalgae cultivation in achieving sustainable energy solutions and mitigating climate change, while also acknowledging the scalability challenges and the necessity for significant economic and energy investments.

Research on the potential of biomass as a chemical energy source

Biomass is an important renewable energy source, derived directly or indirectly from photosynthesis in plants. At present, the world relies heavily on traditional fossil energy, which are hydrocarbons or their derivatives, formed by the accumulation of ancient fossils over time. This paper analyzes and compares the energy produced by various chemical energy sources, as well as their environmental impact and energy efficiency to study the feasibility of substances as bio-energy sources. As energy is indispensable for the survival of human beings, how to discover more green energy and utilize it will become a major challenge for us in the future. Biomass, as a kind of chemical energy has the potential to solve the problem of lack of chemical energy. This paper finally concludes that biomass is feasible as a future chemical energy source, and its super renewable capacity and its nature for solar energy tell us about its huge potential.

Genome-Wide Identification of Sorghum Paclobutrazol-Resistance Gene Family and Functional Characterization of SbPRE4 in Response to Aphid Stress.

Sorghum (Sorghum bicolor), the fifth most important cereal crop globally, serves as a staple food, animal feed, and a bioenergy source. Paclobutrazol-Resistance (PRE) genes play a pivotal role in the response to environmental stress, yet the understanding of their involvement in pest resistance remains limited. In the present study, a total of seven SbPRE genes were found within the sorghum BTx623 genome. Subsequently, their genomic location was studied, and they were distributed on four chromosomes. An analysis of cis-acting elements in SbPRE promoters revealed that various elements were associated with hormones and stress responses. Expression pattern analysis showed differentially tissue-specific expression profiles among SbPRE genes. The expression of some SbPRE genes can be induced by abiotic stress and aphid treatments. Furthermore, through phytohormones and transgenic analyses, we demonstrated that SbPRE4 improves sorghum resistance to aphids by accumulating jasmonic acids (JAs) in transgenic Arabidopsis, giving insights into the molecular and biological function of atypical basic helix-loop-helix (bHLH) transcription factors in sorghum pest resistance.

The stepwise regression analysis method for estimating sorghum production in Karangmojo

Abstract Sorghum is a multifunctional crop that can be utilized as a source of food, feed, and bioenergy. Sorghum is a plant that can adapt to land with optimal conditions, dry land with minimal nutrients and tolerant to pests and diseases. Sorghum has been widely cultivated in Indonesia, one in Gunungkidul, Yogyakarta. Sorghum production is influenced by several agronomic characteristics. The purpose of the study was to determine agronomic variables that affect sorghum production. The research was conducted in Karangmojo, Gunungkidul, Special Region of Yogyakarta, from October 2022 to March 2023. Sorghum varieties were local varieties as well as national superior varieties which are usually planted by local farmers. A total of 10 variables were analysed for their significance level on sorghum yield. The collected data were then processed using a multiple linear regression model (stepwise) using SPSS 16.0. The results showed that of the 10 agronomic variables observed, two variables contributed to the sorghum production, namely panicle weight and days to harvesting. The regression model from stepwise results is y = 8.884 + 0.036x3 – 0.062x10, with R2 = 0.754. This result indicates that the two independent variables are the main variables in determining sorghum production in Karangmojo.

Bioenergy: Paradoxes and dilemmas of a promising energy alternative

The energy production that still moves the world comes in the vast majority from fossil sources. However, according to renewable energy main institutions reports, the sources from which this renewable energy is being generated are hydropower with the highest percentage by far, 41.2%, followed by solar 31.2%, wind 26.4%, bioenergy 4.5%, geothermal 0.4%, and tidal with a minimum percentage of 0.02 %. Therefore, bioenergy is located as another of the promising renewable energies, but still with a very low percentage. As biomass is the main source of bioenergy, it is the renewable energy source with the greatest variety of inputs, for example, biogas from landfill or thermal processes, solid biofuels from wood, municipal waste, and crops or animal waste, and liquid biofuels from conventional and advanced bio gasoline and biodiesel, among others. The purpose of this paper is to explore and analyze the uses of biomass in some Latin American countries, offering a general reflection on this region, and to expand on the various faces of energy injustice suffered by a series of communities, and also the advances that the bioenergy sources could offer; therefore, the structure of this chapter is as follows: first, some general information and facts related to one of its inputs -the steadily growing problem of human waste production- are exposed. Then, a brief description of bioenergy advances in some Latin America countries is offered, to close with the identification of some challenges to be addressed within the domain of bioenergy and with some conclusions.

The sustainable configuration of a circular economy in the agri-food supply chain: a case study of the sugar cane supply chain

PurposeThis paper explores the connection between agri-food supply chains (AFSCs) and levels of circular economy (CE): supply chain (SCs), firm, and product/materials. We aim to answer the following crucial research questions: (1) What are the specific characteristics of the AFSC that affect the implementation of CE? (2) How can the interplay of AFSC characteristics and CE levels be understood and utilised to close the current knowledge gap in CE implementation? (3) How do the CE levels established (in question 2) correspond to the notion of cascade chains? (4) Can the Triple-Bottom Line (TBL) principles be used to report CE impacts in the agri-food industry? (5) What future research directions need to be explored for sustainable CE configuration in AFSCs? Answering these questions expands the knowledge of the implementation of CE in AFSCs, which is crucial for sustainable configuration based on the TBL principles.Design/methodology/approachWe conducted a comprehensive narrative overview (a type of narrative literature review) followed by a case study guided by the cascade chain theory. The sugar cane industry, a significant source of bioenergy that can contribute to sustainable development, was selected for the case study. To validate our findings from the narrative overview, we interviewed five directors from international sugar companies.FindingsOur research has shown that CE can significantly benefit the sugar cane industry. Based on TBL principles, we have developed a framework to achieve sustainable configurations in AFSCs. The framework starts with regenerative agriculture, material reuse, and energy recovery, where different CE levels intersect. This intersection can guide firms to improve decision-making, promote sustainable practices, and inform policymaking across the sugar cane value chain.Research limitations/implicationsNarrative overview has limitations such as potential subjectivity and bias and may not be suitable for generalisation. To mitigate this limitation, we have included a case study to produce a rounded analysis. We have also gathered information from secondary sources, such as reports and company news articles, to prevent biased results.Practical implicationsThis research provides valuable insights to assist companies in aligning their practices with CE levels, leading to sustainable outcomes in AFSCs. The study emphasises the importance of integrating CE levels and AFSC characteristics for policymakers to validate and develop new policies. Through our in-depth analysis of sugar cane SCs, we demonstrate that the various CE levels are interdependent and work in tandem with the unique characteristics of sugar cane, resulting in a sustainable configuration.Originality/valueAnalysing the connectedness between CE levels and AFSC characteristics is crucial to fully understanding the CE sustainable configuration. Unlike other frameworks that only describe the CE concept, the framework presented in this paper clearly explains the implementation of CE in AFSCs. It helps industrial practitioners and policymakers validate current practices and future policies. The paper also highlights future research directions and provides valuable insights.

Metabolite Compounds of Euglena sp. on Mass Cultivation System under MgCl2 and CaCl2 Salt Stress

Euglena sp. is rich in primary and secondary metabolite compounds. This cosmopolitan organism can survive extreme conditions such as salt stress because it can increase cell growth rate and metabolism. This mass cultivation research uses an open pond system that is very vulnerable to contamination, so variations in salinity of MgCl2 and CaCl2 are used as treatments because they minimize contamination. The research examines the correlation of salt types to cell growth rate, biomass, monosaccharide profile, paramylon, pigment, and lipid of Euglena sp. Research methods include preparation, medium making, mass cultivation, and measurement of test parameters. Data were analyzed through One-Way ANOVA followed by DMRT, with a 5% confidence level. Based on the research result, the highest value of biomass and paramylon content and productivity at CaCl2 treatment with consecutive values 0.013 ± 0.001, 0.002 ± 0.000, 9.556 ± 0.070, and 0.149 ± 0.019. Monosaccharides analysis produced in the form of sucrose, glucose, fructose, and arabinose amounted to &lt; 10 ppm. CaCl2 treatment produced the highest sucrose of 6251 ppm. The highest chlorophyll-a and carotenoid pigments are located at CaCl2 treatment, respectively (1.698 ± 0.051)b and (0.500 ± 0.032)b. At the same time, the salt treatment has a significant effect on lipid content. To summarize, almost all metabolite compounds were highest in CaCl2 treatment. This research is expected to contribute to developing the Euglena sp. mass cultivation system as a source of bioenergy and biomaterials.