Biodegradation Of Reactive Black Research Articles

Biodegradation of Reactive Black 5 by Aeromonas hydrophila strain isolated from dye-contaminated textile wastewater

Abstract Aeromonas hydrophila isolate showed the best decolorization of Reactive Black 5 (RB5) at the concentration of 100 mg L−1 on modified mineral salt medium among 42 bacterial isolates. Optimization of parameters for RB5 dye decolourization was studied under static condition. Under optimized condition, decolorization efficiency of RB5 by A. hydrophila was found to be 76% at 100 mg L−1 within 24 h. The optimum pH and temperature for the decolorization was 7 and 35 °C respectively. Biodegradation and decolorization of RB5, was monitored by UV–Vis spectrophotometry, Thin Layer Chromatography, Fourier Transform Infrared Spectroscopy and Gas Chromatography Mass Spectrometry analysis. The study has confirmed the potential of A. hydrophila isolated from textile effluent in degradation of RB5 and opened scope for future analysis in the treatment of textile effluent.

Salinity effects on biodegradation of Reactive Black 5 for one stage and two stages sequential anaerobic aerobic biological processes employing different anaerobic sludge

In this study the effect of NaCl, normally found in dye bath wastewaters employing reactive azo dyes, on the performance of sequential anaerobic–aerobic processes for treatment of Reactive Black 5 (RB5) containing media, with concentration in the range 100–500 mg L−1, was investigated. Three possible scenarios of the sequential anaerobic–aerobic process, namely two stage process and one stage processes employing either anaerobic or activated sludge, were considered. The results showed a statistically significant enhancement of the anaerobic decolourisation efficiency as a result of the addition of 30 g L−1 NaCl to the RB5 containing media for two stage processes and one stage processes employing anaerobic sludge. NaCl at 30 g L−1 concentration also inhibited aerobic colour formation during two stage processes whereas it prevented aerobic decolourisation during one stage processes. HPLC and UV Vis analysis indicated that during anaerobic phase/stage the majority of azo bonds in RB5 molecules cleave whereas the hydrophobicity/MW of the resulting dye reduction metabolites decreases. The same analysis revealed partial mineralisation of RB5 reduction metabolites under aerobic conditions. The results of the present work also showed that the effect of salt on anaerobic decolourisation efficiency, TVFA and methane production was dependent on the exposure history of anaerobic sludge.

<b>A bootstrapped neural network model applied to prediction of the biodegradation rate of reactive Black 5 dye</b> - doi: 10.4025/actascitechnol.v35i3.16210

Current essay forwards a biodegradation model of a dye, used in the textile industry, based on a neural network propped by bootstrap remodeling. Bootstrapped neural network is set to generate estimates that are close to results obtained in an intrinsic experience in which a chemical process is applied. Pseudomonas oleovorans was used in the biodegradation of reactive Black 5. Results show a brief comparison between the information estimated by the proposed approach and the experimental data, with a coefficient of correlation between real and predicted values for a more than 0.99 biodegradation rate. Dye concentration and the solution’s pH failed to interfere in biodegradation index rates. A value above 90% of dye biodegradation was achieved between 1.000 and 1.841 mL 10 mL-1 of microorganism concentration and between 1.000 and 2.000 g 100 mL-1 of glucose concentration within the experimental conditions under analysis.

Biodegradation of Reactive Black 5 and bioregeneration in upflow fixed bed bioreactors packed with different adsorbents

AbstractBACKGROUND: Unutilized dyes present a significant environmental problem. Recently, biological activated carbon has been used extensively for the removal of gas and wastewater pollutants but its use in dye removal is still limited. The system provides simultaneous adsorption of pollutants and biodegradation/biotransformation of pollutants in a single reactor, and showed high efficiency and a long working life.RESULTS: The adsorption of Reactive Black 5 by three selected adsorbents, F400, bone char and bamboo activated carbon, was studied. The relationships between adsorption, desorption, bioregeneration and the effect of nutrient on dye adsorption was assessed. The potential value of using adsorbents as a microbial support in the biodegradation of Reactive Black 5 was investigated. Biodegradation was greatly enhanced in the fixed bed bioreactors and complete decolorization was achieved in all the reactors studied with hydraulic retention time less than 15 minutes. The bioregenerated adsorption capacity of bamboo activated carbon in the bioreactor was found to be the highest.CONCLUSION: Decolorization of Reactive Black 5 was significantly enhanced in the bioreactors. Results showed that the immobilized bacteria can increase the desorbability of the adsorbed dye, which is important for practical operation, and that bamboo activated carbon demonstrated potential as a packing material in fixed bed bioreactors. Copyright © 2010 Society of Chemical Industry