Biodegradation Of Chlorpyrifos Research Articles

Biodegradation of Chlorpyrifos in Paddy Rice Soil in the Mekong Delta, Vietnam

Biodegradation of Chlorpyrifos in Paddy Rice Soil in the Mekong Delta, Vietnam

Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milk

Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years, though the biodegradation mechanism and derived intermediate products remain unclear. This study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide, chlorpyrifos, in milk by Lactobacillus delbrueckii ssp. bulgaricus using gas chromatography- tandem mass spectrometry (GC-MS/MS) combined with transcriptome analysis. After the strain was cultured for 20 h in the presence of chlorpyrifos, differential expressions of 383 genes were detected, including genes probably implicated during chlorpyrifos degradation such as those related to hydrolase, phosphoesterase, diphosphatase, oxidoreductase, dehydratase, as well as membrane transporters. GC-MS/MS analysis revealed the changes of secondary metabolites in L. bulgaricus during milk fermentation due to chlorpyrifos stress. 6-Methylhexahydro-2H-azepin-2-one, 2,6-dihydroxypyridine and methyl 2-aminooxy-4-methylpentanoate as intermediates, along with the proposed pathways, might be involved in chlorpyrifos biodegradation by L. bulgaricus.

Enhancement of Biodegradation of Chlorpyrifos and Carbaryl of Gill, Muscle, Liver Tissues of Cyprinus Carpio

Enhancement of Biodegradation of Chlorpyrifos and Carbaryl of Gill, Muscle, Liver Tissues of Cyprinus Carpio

Enhanced biodegradation of chlorpyrifos in the presence of sub-inhibitory concentration of ZnONPs by Pseudomonas sp. CF7b

Chlorpyrifos, a widely used organophosphate pesticide, poses significant environmental and health risks due to its prolonged persistence and toxicity. Hence the biodegradation of chlorpyrifos has immense significance for its effective removal, and the present study has been designed on the management of chlorpyrifos pollution through an innovative bioremediation approach. Here, Pseudomonas sp. CF7b isolated from the soil has been investigated for chlorpyrifos removal in the presence of sub-inhibitory concentrations (sub-MIC) of zinc oxide nanoparticles (ZnONPs). For this, chlorpyrifos-tolerant bacteria were initially isolated from the soil and out of the 29 bacterial isolates obtained, Pseudomonas sp. CF7b was selected for the detailed study. The selected organism was then treated with chlorpyrifos in the presence of sub-inhibitory concentrations of ZnONPs. The quantitative reduction of chlorpyrifos under the experimental conditions was further analyzed using gas chromatography-mass spectrometry (GC-MS). Here, chlorpyrifos treated in the presence of both Pseudomonas sp. CF7b and sub-MIC ZnONPs was found to undergo enhanced reduction when compared with the quantitative changes observed for the treatment containing the organism or ZnONPs alone. After 120 hof incubation, a 90.9 % reduction was observed in chlorpyrifos for the treatment containing both Pseudomonas sp. CF7b and ZnONPs. In comparison, the reduction was 81.6 % for samples treated with Pseudomonas sp. CF7b alone and 53.4 % for samples treated with ZnONPs alone. The observation from the current study provides valuable insights into the role of nanomaterials in enhancing the microbial action against environmental pollutants and hence opens up the scope for the development of novel sustainable methods for the environmental management of pesticides.

Comparative evaluation of biodegradation of chlorpyrifos by various bacterial strains: Kinetics and pathway elucidation

The present study focused on the isolation and identification of CP and TCP bacteria degrading bacteria from the rhizospheric zone of aromatic grasses i.e. palmarosa (Cymbopogon martinii (Roxb. Wats), lemongrass (Cymbopogon flexuosus) and vetiver (Chrysopogon zizaniodes (L.) Nash.). So that these isolates alone or in combination with the vegetation of aromatic grasses will be used to clean up CP-contaminated soils. The study also explored enzymatic activities, CO2 release, dechlorination potential, and degradation pathways of bacterial strains. A total of 53 CP-tolerant bacteria were isolated on their physical characteristics and their ability to degrade CP. The ten highly CP-tolerant isolates were Pseudomonas aeruginosa Pa608, three strains of Pseudomonas hibiscicola R4–721 from different rhizosphere, Enterococcus lectis PP2a, Pseudomonas monteilii NBFPALD_RAS131, Enterobacter cloacae L3, Stenotrophomonas maltophilia PEG-390, Escherichia coli ABRL132, and Escherichia coli O104:H4 strain FWSEC0009. The CO2 emission and phosphatase activities of the isolates varied from 3.1 to 8.6 μmol mL−1 and 12.3 to 31 μmol PNP h−1, respectively in the CP medium. The degradation kinetics of CP by these isolates followed a one-phase decay model with a dissipation rate ranging from 0.048 to 0.41 d−1 and a half-life of 1.7–14.3 days. The growth data fitted in the SGompertz equation showed a growth rate (K) of 0.21 ± 0.28 to 0.91 ± 0.33 d−1. The P. monteilii strain had a faster growth rate while E. coli ABRL132 had slower growth among the isolates. The rate of TCP accumulation calculated by the SGompertz equation was 0.21 ± 0.02 to 1.18 ± 0.19 d−1. The Pseudomonas monteilii showed a lower accumulation rate of TCP. Among these, four highly effective isolates were Pseudomonas aeruginosa Pa608, Pseudomonas monteilii NBFPALD_RAS131, Stenotrophomonas maltophilia PEG-390, and Pseudomonas hibiscicola R4–721. Illustrations of the degradation pathways indicated that the difference in metabolic pathways of each isolate was associated with their growth rate, phosphatase, dehydrogenase, oxidase, and dechlorination activities.

Biodegradation of Chlorpyrifos by Microbes: A Review

Chlorpyrifos (CP) is a widely used organophosphate pesticide known for its recalcitrant nature, raising concerns about potential ecological and health impacts due to its toxicity. Many plants and animals are contaminated with this pesticide. Microbial biodegradation offers an environmentally friendly and effective method to remove CP from the environment and mitigate its impacts, especially given its low cost, particularly when bioremediation is conducted on-site. Different types of microbial species have been found to function under various environmental conditions, with some, like Pseudomonas nitroreducens PS-2 and Pseudomonas aeruginosa (NCIM 2074), showing promising results with degradation rates of up to 100%. However, challenges exist, such as partial degradation caused by the presence of metabolites, and the recalcitrant nature of CP, which can impede microbes' ability to effectively degrade its hydrocarbon ring. Overall, a combination of approaches, such as microbial and algal methods, or the discovery of new microbial strains, can help overcome these challenges and further enhance the long-term viability of this technique.

Chlorpyrifos Biodegradation Study in Egyptian Agricultural Soil

Chlorpyrifos Biodegradation Study in Egyptian Agricultural Soil

Biodegradation of chlorpyrifos by charcoal–alginate immobilized bacterial consortium isolated from pesticide-polluted Kuzhikandam Creek, Kerala, India

Chlorpyrifos, one of the most frequent organophosphorus pesticides, has been linked to major health problems and widespread environmental degradation. It is imperative to remove this herbicide from its point source of contamination since the risk of their off-site migration poses a health concern to non-target organisms. The objective of the present study was to investigate the biodegradation of chlorpyrifos 20% emulsifiable concentration (EC) utilizing a consortium of bacteria that had previously been isolated from a pesticide-contaminated site and are chlorpyrifos tolerant (S27I and S28I). Free cells and immobilized cells with chlorpyrifos as the only carbon source were used to analyze the biodegradation. Charcoal–alginate and kaolin–alginate were the immobilization matrices employed in the investigation. By using gas chromatography-mass spectrophotometer analysis, the degradation of chlorpyrifos was investigated. When compared to free cells, immobilized bacterial isolates showed the greatest amount of biodegradation in the investigation. Consortium (C1) among the isolates degraded chlorpyrifos most quickly compared to S27I and S28I. The study also examined the toxicity of chlorpyrifos at doses of 10, 25, 50, 75, and 100 ppm on the green gram seeds’ morphological parameters. Increased chlorpyrifos concentrations resulted in appreciable variations in the germination percentage, shoot length, and root length. The green gram germination study was used to evaluate the detoxification of chlorpyrifos following its breakdown.

English

Regular use of organophosphate pesticides (OPPs) such as chlorpyrolifos in agriculture results in a variety of environmental issues, including groundwater contamination, surface water pollution, enhanced floral and faunal resistance, soil acidity, reduced soil fertility, and nitrate leaching. The objective of the current study was to identify and isolate bacterial strains from native soil that could degrade chlorpyrifos and had high pesticide resistance. A total of 60 distinct microbial strains were isolated from the soil samples and subsequently evaluated for their capacity to degrade chlorpyrifos. Serratia liquefaciens, one of the strains, was discovered to be the most effective among them for degrading chlorpyrifos. It was subsequently examined for the biodegradation of chlorpyrifos under various environmental conditions and was then identified using 16S rRNA sequencing. The strain S. liquefaciens degraded more than 90% of 50 mg L-1 of chlorpyrifos. The strain performed better when urea and sucrose were used as nitrogen and carbon sources, respectively. At a temperature of 35 °C and a basic pH range, the strain functioned exceptionally well. The finding demonstrated that the bacterial strain S. liquefaciens, which was isolated from agricultural soil, is a top contender for cleaning up OPP-contaminated soil.

Biodegradation of chlorpyrifos by soil bacteria and their effects on growth of rice seedlings under pesticide-contaminated soil

Biodegradation of chlorpyrifos by soil bacteria and their effects on growth of rice seedlings under pesticide-contaminated soil

Biodegradation of Chlorpyrifos by Soil-derived Aerobic Consortia and Bacterial Isolates

Biodegradation of Chlorpyrifos by Soil-derived Aerobic Consortia and Bacterial Isolates

Biodegradation mechanism of chlorpyrifos by halophilic bacterium Hortaea sp. B15

For decades, most of the developing nations have relied on chlorpyrifos for insecticidal activity in the agriculture sector. It is a common chlorinated organophosphorus pesticide that has been widely used to control insects to protect plants. This study aimed to investigate the effects of environmental characteristics such as salinity, pH, temperature, and surfactant on Hortaea sp. B15 mediated degradation of chlorpyrifos as well as enzyme activity and metabolic pathway. The highest bacterial growth (4.6 × 1016 CFU/mL) was achieved after 20 h of incubation in a 100 mg/L chlorpyrifos amended culture. The fit model and feasible way to express the chlorpyrifos biodegradation kinetics in normal condition and optimized was a first-order rate equation, with an R2 value of 0.95–0.98. The optimum pH for chlorpyrifos biodegradation was pH 9, which resulted in a high removal rate (91.1%) and a maximum total count of 3.8 × 1016 CFU/mL. Increasing the temperature over 40 °C may inhibit microbial development and biodegradation. There was no significant effect of culture salinity on degradation and bacterial growth. In the presence of non-ionic surfactant Tween 80, the maximum chlorpyrifos degradation (89.5%) and bacterial growth (3.8 × 1016 CFU/mL) was achieved. Metabolites such as 3,5,6-trichloropyridin-2-ol and 2-pyridinol were identified in the Hortaea sp. B15 mediated degradation of chlorpyrifos. According to the findings, Hortaea sp. B15 should be recommended for use in the investigation of in situ biodegradation of pesticides.

Biodegradation of chlorpyrifos by bacterial strains isolated from Lebanese soil and its association with plant growth improvement

Chlorpyrifos poses a danger to the environment as a result of its toxicity and endurance in soil and water. Bioremediation of pesticide-polluted soils is one of the most promising approaches for removing contaminants from the environment. This study aimed to isolate and identify chlorpyrifos-degrading bacterial strains and test their biodegradation capabilities and their roles in plant growth. The 16S rDNA gene sequencing identified the two efficient chlorpyrifos-degrading bacterial strains as Stenotrophomonas maltophilia (RS1) and Acinetobacter calcoaceticus (RS3), which significantly (p < 0.01) degraded 71.3% and 73.5% of chlorpyrifos in the liquid medium, respectively, compared to 80% degradation (p < 0.01) when cultured as a consortium within 48 h. The bioremediation of artificially chlorpyrifos-contaminated soils varied between 84.4% and 90.11% (p < 0.01), depending on the used bacterium and soil sterilization status. Furthermore, the enzymes embroiled in the degradation mechanism were sprinkled all over the cells but with different portions. Experiments on plant growth revealed that chlorpyrifos hurt lettuce growth. The presence of RS1 and RS3, on the other hand, demonstrated considerable lettuce growth-enhancing traits in terms of germination percentages, shoot and root lengths and weights. Our isolates could be promising candidates in the bioremediation of chlorpyrifos-contaminated sites in conjunction with their contribution as biofertilizers.

Chlorpyrifos Degradation in Semi-Arid Soil by Pseudomonas fluorescens Strain CD5 Isolated from Manured Soil

ABSTRACT The present paper focused on investigating the potential of indigenous Pseudomonas fluorescens strain CD5 isolated from manure-enriched soil as a bioremediation agent for chlorpyrifos biodegradation in semi-arid soil of Bathinda district of Punjab, India. The soil had sandy loam texture with slightly alkaline pH and low organic matter content of 0.36%. The growth pattern, alkaline phosphatase enzyme production and chlorpyrifos utilization capability of Pseudomonas fluorescens strain CD5 were screened in mineral salt medium and the results revealed degradation of 78.19% of 500 mg L−1 chlorpyrifos within 15 days. The pattern of chlorpyrifos degradation was further studied in natural conditions of semi-arid soil by the Pseudomonas fluorescens strain CD5 and photolysis by sunlight for a period of 105 days. The biodegradation of chlorpyrifos at 500 mg kg−1 sterile soil by Pseudomonas fluorescens strain CD5 was 59.68% in comparison to 26% of photodegradation and enhancing total degradation. The residual chlorpyrifos in sterile soil after biodegradation by Pseudomonas fluorescens strain CD5 under natural light condition was 14.33%. The alkaline phosphatase enzyme activity in soil was found positively correlated with the chlorpyrifos degradation. The Pseudomonas fluorescens strain CD5 can be an effective candidate for the bioremediation of chlorpyrifos-contaminated soil.

Pesticide degradation in an integrated constructed wetland: Insights from compound-specific isotope analysis and 16S rDNA sequencing

Carbon isotope analysis and the 16S rDNA sequencing were adopted to investigate the degradation process of chlorpyrifos during its transport in the integrated constructed wetland (ICW). Firstly, the extent of concentration decrease of chlorpyrifos was examined, and the removal efficiency in the first 36 h was found to be the highest. The removal rate reached 96.83 % after 96 h, and this process fit to the first-order kinetic model, with a kinetic constant (k) of 0.066 h−1. A significant carbon isotope fractionation was observed, with a change of the δ13C values from −26.54 ± 0.07 ‰ to −25.41 ± 0.08 ‰. The average chlorpyrifos biodegradation proportion reached 71.23 % (60.42 %–85.04 %), and it was predicted that about 11.79 %–36.41 % of chlorpyrifos removal in the ICW was attributed to abiotic factors. The outlet of the subsurface flow constructed wetland saw the highest D∗/B∗ value (1.38–3.88), indicating that the remaining fraction of dilution was much more significant than that of degradation in this period. The top 20 phyla of microbial community were identified in the ICW. Proteobacteria was the most dominant phylum, accounting for >40 % of the bacterial communities in all sampling locations. Acidobacteria and Bacteroidetes were the second and third dominant phyla. At the genus level, the microbial community composition differed more greatly in every stage of the ICW, and the spatial distribution difference was quite significant in the ICW. This study is important to figure out the migration and transformation of chlorpyrifos when the ICW was adopted as a removal tool for organic micro-pollutants, and more similar studies could be carried out in the future to promote the evaluation of pollutant removal capacity of the ICWs, and to further develop the application of stable isotope analysis of compounds in the natural environment.

Chlorpyrifos biodegradation by a Locally Isolated Bacterium Pseudomonas sp. B5-2

Chlorpyrifos has been considered as one of the most frequently used organophosphorus insecticides in agriculture worldwide. There has been considerable public concern regarding its neurotoxicity and its genotoxicity on mammals, aquatic organisms, and other non-target species. In this study 23 bacterial isolated strains from different origins were tested for their ability to resist and subsequently degrade chlorpyrifos (CP). Seven of these strains showed a resistance to up to 1000mg/L CP on solid minimal mineral salt medium (MMSM) and the strain Pseudomonas sp. B5-2 was selected for further analysis after a selection on liquid MMSM. The effect of different concentrations of CP on the growth of the selected strain was tested, and 200 mg/L was the highest concentration that supports its growth. The ability of the selected strain to eliminate CP was investigated in the presence and in the absence of glucose as an external carbon source. The results showed that the strain utilized chlorpyrifos (200 mg/L) as the sole source of carbon better than in presence of an external carbon source (glucose) with an elimination rate of 67.06% and 14.61%, respectively. These results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide waste in the environment.

Enterobacter sp. SWLC2 for biodegradation of chlorpyrifos in the aqueous medium: Modeling of the process using artificial neural network approaches

Chlorpyrifos is a widely used organochlorine pesticide. The unsystematic use of pesticides for the restrain of soil-borne pests and bacterium causes pollution of ecosystems and harming the health of animals and humans. Moreover, due to high persistence, mobility, and higher toxicity in soil environments, it causes severe destruction of species. Therefore the investigation of the degradation process of chlorpyrifos polluted locations to diminish its lethal effect is important. With this objective, the present study reports the efficiency of new isolated bacterial strain SWLC2 for chlorpyrifos degradation in an aqueous medium and the biotic/abiotic factors influencing the process and their optimization using statistical Taguchi design and artificial neural network (ANN) approaches. Results revealed a higher efficiency of bacterium SWLC2 for chlorpyrifos degradation at optimal environmental and general conditions (800 μL inoculum, glucose (97%), arginine (89%), and succinic acid (83%)). External supplements (carbon and nitrogen sources) had stimulating effects on chlorpyrifos biodegradation depending on the concentration of supplemented substrate. Chlorpyrifos biodegradation by SWLC2 also varied as the initial concentration of the tested compound changes, following first-order kinetics. The finding provides a basic understanding of the application of the bacteria strain SWLC2 in the bioremediation of soils contaminated with chlorpyrifos pesticide. ANN approaches have achieved an average R2 equal to 0.98–0.99 in the prediction of chlorpyrifos degradation considering the affecting biotic/abiotic factors.

Biodegradation of Chlorpyrifos by Some Bacterial Strains and Screening Their Degraded Genes

Biodegradation of Chlorpyrifos by Some Bacterial Strains and Screening Their Degraded Genes

Biodegradation of chlorpyrifos by bacterial genus pseudomonas putida

Chlorpyrifos is an solid organophosphorus pesticide widely used in agriculture. It is relatively stable to hydrolysis in neutral pH and acidic aqueous solutions. Stability decreases with increasing pH. Chlorpyrifos practically insoluble in water, but highly soluble in most organic solvents such as acetone xylene and methyl bromide. It is an effective skin, stomach and respiratory insecticide and is effective against aphids, spider mites, soil bugs and house pests. It is also highly toxic bees and fish. Most pesticides are complex organic molecules and are persistent in the environment, either biota or accumulate in the environment. In pesticide-used areas, the pesticide itself or its residues are transported by rain and irrigation water and mixes into groundwater. The effects caused by the pesticide are closely related to the level of accumulation. During the studies, contamination of chlorpyrifos has been found about 24 km from the site of its application. There are many physico-chemical and biological approaches to remove organophosphorus pesticides from the ecosystem, among them most promising is biodegradation. In this study, the biodegradation potential of chlorpyrifos with P. Putida was investigated in the batch stirred reactor. In the optimum conditions, the maximum pesticide removal rate was determined as 1.51 mg/g. d. m.o.h.

Biocatalytic degradation of organophosphate pesticide from the wastewater and hydrolytic enzyme properties of consortium isolated from the pesticide contaminated water

The indiscriminate application of various pesticides leads to toxicity to the humans, animals, fishes and threatens the environment and ecosystem. The present study was aimed to investigate pesticide degrading bacteria from the pesticide contaminated sample and to localize organophophate hydrolase activity from the bacteria. Sediment sample was selected as the source of microorganism for the degradation of chlorpyrifos. Enterobacter aerogenes CP2 and Streptococcus pyogenes CP11 isolated from the contaminated sample removed 77 ± 1.8%, 74.2 ± 3.1 chlorpyrifos. These strains have the potential to utilize pesticide as the source of carbon and energy. The pesticides inoculated with both CP 2 and CP 11 enhanced biodegradation of chlorpyrifos at optimized condition. E. aerogenes CP2 and S. pyogenes CP11 produced organophosphate hydrolase activity and localized enzyme biosynthesis. Organophosphate hydrolase activity was high in intracellular, followed by outer membrane and extracellular sample for both bacteria. The treated wastewater has no impact on the seed germination indicated normal cell division, cell elongation and indole-3 acetic acid synthesis. The strain CP2 has the rapid rate of organophosphate degradation among Enterobacter species.