Biodegradable Fraction Research Articles

Bayesian inversion of bacterial physiology and dissolved organic carbon biodegradability on water incubation data

In aquatic ecosystems, dissolved organic carbon (DOC) plays a significant role in the global carbon cycle. Microorganisms mineralize biodegradable DOC, releasing greenhouse gases (carbon dioxide, methane) into the atmosphere. Extensive research has focused on the concentrations and biodegradability of DOC in aquatic systems worldwide. However, little attention has been given to uncertainties regarding the physiological characteristics of heterotrophic bacteria, which are crucial for biogeochemical modeling. In this study, the physiological properties of heterotrophic bacteria and the properties of DOC biodegradability in water are inferred through a Bayesian inversion approach. To achieve this, treated and natural water samples collected from the Seine River basin, were inoculated and incubated in laboratory. During incubation, the concentrations of DOC and heterotrophic bacteria biomass were measured. Then, a multiple Monte Carlo Markov Chains method and the HSB model (High-weight polymers, Substrate, heterotrophic Bacteria) are applied on the water incubation data. The results indicate a higher biodegradable fraction of DOC in natural water compared to treated water and significant variability in the fraction of fast biodegradable DOC within 5 days in both water samples. The significant variability highlights the uncertainties/challenges in the HSB model parameterization. The seven water samples used in the paper serve as a proof of concept. They are from various origins and display the potential of the method to identify parameter values in a large range of values. Because mortality rate of heterotrophic bacteria at 20 ∘C (kd20) showed a remarkable stability at 0.013 h−1, we considered that this parameter can be fixed at this value. The maximum growth rates at 20 ∘C (μmax20) was 0.061 h−1 while optimal growth yield (Y) estimated at 0.34 for treated water and at 0.25 for natural water. All these parameter values are well in accordance with previous determinations.

Enhancing the High-Solid Anaerobic Digestion of Horticultural Waste by Adding Surfactants.

The influence of adding surfactants on the performance of high-solid anaerobic digestion of horticultural waste was extensively investigated in batch systems. Adding Tween series and polyethylene glycol series non-ionic surfactants had positive effects on biogas production, resulting in 370.1 mL/g VS and 256.6 mL/g VS with Tween 60 and polyethylene glycol 300 at a surfactant-to-grass mass ratio of 0.20, while the biogas production of anaerobic digestion without surfactants was 107.54 mL/g VS. The optimal and economically feasible choice was adding Tween 20 at a ratio of 0.08 g/g grass in high-solid anaerobic digestion. A kinetics model reliably represented the relationship between surfactant concentration and biogas production. The mechanism of surfactants working on lignocellulose was investigated. The improvement in high-solid anaerobic digestion by adding surfactants was attributed to the interaction between lignocelluloses and surfactants and the extraction of biodegradable fractions from the porous structure. An economic analysis showed that adding Tween 20 was likely to make a profit and be more feasible than adding Tween 60 and polyethylene glycol 300. This study confirms the enhancement in biogas production from horticultural waste by adding non-ionic surfactants.

Microbial stratification and DOM removal in drinking water biofilters: Implications for enhanced performance

Biofiltration is a low-cost, low-energy technology that employs a biologically activated bed of porous medium to reduce the biodegradable fraction of the dissolved organic matter (DOM) pool in source water, resulting in the production of drinking water. Microbial communities at different bed depths within the biofilter play crucial roles in the degradation and removal of dissolved organic carbon (DOC), ultimately impacting its performance. However, the relationships between the composition of microbial communities inhabiting different biofilter depths and their utilisation of various DOC fractions remain poorly understood. To address this knowledge gap, we conducted an experimental study where microbial communities from the upper (i.e., top 10 cm) and lower (i.e., bottom 10 cm) sections of a 30-cm long laboratory-scale biofilter were recovered. These communities were then individually incubated for 10 days using the same source water as the biofilter influent. Our study revealed that the bottom microbial community exhibited lower diversity yet had a co-occurrence network with a higher degree of interconnections among its members compared to the top microbial community. Moreover, we established a direct correlation between the composition and network structure of the microbial communities and their ability to utilise various DOM compounds within a DOM pool. Interestingly, although the bottom microbial community had only 20 % of the total cell abundance compared to the top community at the beginning of the incubation, it utilised and hence removed approximately 60 % more total DOC from the DOM pool than the top community. While both communities rapidly utilised labile carbon fractions, such as low-molecular-weight neutrals, the utilisation of more refractory carbon fractions, like high-molecular-weight humic substances with an average molecular weight of more than ca. 1451 g/mol, was exclusive to the bottom microbial community. By employing techniques that capture microbial diversity (i.e., flow cytometry and 16S rRNA amplicon sequencing) and considering the complexities of DOM (i.e., LCOCD), our study provides novel insights into how microbial community structure could influence the microbial-mediated processes of engineering significance in drinking water production. Finally, our findings could offer the opportunity to improve biofilter performances via engineering interventions that shape the compositions of biofilter microbial communities and enhance their utilisation and removal of DOM, most notably the more classically humified and refractory DOM compound groups.

Surfactant-assisted thermal hydrolysis of sewage sludge: Biomolecule extraction and its potential for protease production

Efficient management of sewage sludge from wastewater treatment plants (WWTPs) are crucial to obtain industrial value-added products and reducing environmental impact. Thermal hydrolysis, coupled with surfactants to enhance solubilisation, offers a promising approach for sewage sludge treatment. This study assessed the impact of four surfactants: sodium dodecyl sulphate (SDS), sodium dodecylbenzenesulfonate (SDBS), cetyl trimethyl ammonium chloride (CTAC), and tetraethylammonium chloride (TAC) on thermal hydrolysis of sewage sludge. The addition of anionic surfactants (SDS and SDBS) significantly increased biomolecule production, with proteins, humic-like substances, carbohydrates, and DNA concentrations reaching 6018 ± 28 mg/L (306 mg/gVSSo), 2496 ± 103 mg/L (127 mg/gVSSo), 1822 ± 4 mg/L (93 mg/gVSSo), and 389 ± 3 mg/L (20 mg/gVSSo), respectively, after 155 min of thermal hydrolysis. Low levels of surfactants (10 and 50 mg/gVSSo) also led to a substantial increase in the readily biodegradable fraction of hydrolysed sewage sludge, reaching up to 90 %. Additionally, surfactant-assisted hydrolysed sewage sludge proved to be a favourable substrate for protease production using Bacillus licheniformis. The optimal enzymatic activity of 678 ± 14 U/mL was achieved when CTAC was added at a concentration of 10 mg/gVSSo. In summary, this study evaluated an innovative approach to enhance the solubilisation of biomolecules from sewage sludge using surfactant-assisted thermal hydrolysis. This methodology not only positively affected biodegradability but also proved to be suitable for potentially producing valuable industrial products like proteases through fermentation with B. licheniformis, thus offering a new strategy for the sustainable treatment of sewage sludge.

Disintegrated Waste-Activated Sludge (NO2/FNA Method) as a Source of Carbon for Denitrification in the Mainstream of a WWTP

The deficiency of readily biodegradable organic carbon can be a significant limitation to effective nitrogen removal during wastewater denitrification. Waste-activated sludge (WAS) is a source of carbon produced directly at wastewater treatment plants (WWTPs). Raw WAS has a large molecular weight and complex chemical structure molecules that are not easily available for microorganisms. In this study, easily biodegradable organic fractions were released using pH control and/or nitrites and nitric acid (NO2/FNA). The obtained results indicated that WAS can be a sufficient carbon source for denitrification in WWTPs that are at risk of minor effluent violations. The implementation of WAS disintegration with the use of pH control and NO2/FNA allowed for the denitrification of an additional 0.5 and 0.8 mgN-NO3/L. WAS disintegration, besides being a source of carbon generation, reduces the volume of sludge and leads to the implementation of a closed-loop system.

The Age-Induced Quality Shift of Vermicompost Influenced the Biodegradation Kinetics in Soils

ABSTRACT A study was conducted to identify the effects of aging on the biodegradability of vermicomposts in soil. Two solid wastes, including cow manure (CM) and wheat residues (WR), were subjected to the vermicomposting. The products of the various ages were mixed with Lavark and Langroud soils and incubated for 45 days. Results indicated that the aging effects led to an increase in the lignin (LG) content. Potentially, mineralizable C was decreased from 5308 mg C kg−1 for fresh substrates to 1643 mg C kg−1 for aged CM vermicompost in the Lavark soil. The decreasing trend was also observed for the Langroud soil. Similar behavior was also shown for the soils when treated with WR vermicomposts of different ages. The age of vermicomposts was negatively correlated with potentially mineralizable C as well as LG and C/N ratio. Multiphase decomposition was observed, in particular when the products of the early ages were used. The aging of CM and WR induced a decrease in the number of decomposition phases. Overall, by aging, the less biodegradable fraction (lignin) accumulates, and the patterns of decomposition tend to decrease the number of decomposition phases or decrease the last phase biodegradation rate constant.

Overview of municipal solid waste management in sub-tropical climatic region of North Eastern India.

Municipal solid waste management (MSWM) is perceived as a global issue regardless of the place of waste generation. The amount of unmanaged waste is increasing rapidly, along with its impact on the environment and human health. In hilly areas, specifically the North Eastern Region (NER) states of India, due to the unique topography coupled with socio-economic factors, there are inadequate waste management practices marked by insufficient infrastructure, minimal research studies, and limited data availability. This paper comprehensively reviews the existing status of MSWM practices and waste treatment technologies, identifies the challenges, and discusses the prospective approaches for MSWM in NER states of India. NER, is characterized by its hilly terrain and has the most diverse demographic profile in the country. The study highlights the notable increase in waste generation in the urban population in NER. The total amount of waste generated in NER is about 2907 tons per day, with a collection rate of 86.96%, treatment at 31.09%, and landfilling at 33.67%. The biodegradable fraction makes up the majority of waste composition (more than 50%) in NER, followed by recyclables and inert. The existing MSWM consists of waste collection, transportation, and disposal with limited source segregation and treatment. All the states of NER practice open dumping and burning as the primary waste treatment and disposal system. The study discusses the challenges and prospects to ensure effective MSWM in NER. This review is a region-specific study that considers cultural diversity, topography, and socio-economic dynamics. The outcome of this review will be helpful to the researchers and policymakers in making appropriate waste management plans and improve the MSWM system in NER.

Direct application of chemically enhanced primary treatment in a municipal wastewater treatment plant: A case study

A feasible and efficient implementation of an Fe-based chemically enhanced primary treatment (CEPT) in a large conventional wastewater treatment plant (WWTP) was thoroughly evaluated. The influence of the velocity gradient and hydraulic retention time in the coagulation and flocculation stages, as well as the coagulant (FeCl3) dosage, were evaluated at lab-scale. Standard Jar Tests were performed to simulate CEPT, and the results were compared with those obtained by primary settling without coagulant. The increase in FeCl3 dosage and flocculation hydraulic retention time (HRTF) increased the primary settling COD removal capacity between 6.6% and 18.1%, highlighting its effect on the easily biodegradable COD fraction. The COD removal capacity increased up to 16.8% when working with an HRTF of less than 4 min, facilitating CEPT direct application in conventional WWTPs, avoiding the need to incorporate flocculation chamber, with a corresponding decrease in the associated implementation cost. The presence of residual Fe in the primary settling effluent and an increase in the primary sludge volume were the main drawbacks of the process. The coagulation and flocculation velocity gradients or the coagulation hydraulic retention time did not significantly influence the process.

Ozonation and Changes in Biodegradable Organic Substances in Drinking Water Treatment: The Future of Green Technology

Studies were carried out to assess changes in biodegradable dissolved organic carbon (BDOC) and assimilable organic carbon (AOC) in groundwater and surface waters after two processes: ozonation and ozonation/UV. The tested water was in contact with O3 firstly for 4 and secondly for 15 min. Three doses of disinfectant were used: 1.6 mg/L, 5.0 mg/L, and 10.0 mg/L. The UV radiation time was 10 and 30 min. The greatest change in AOC and BDOC for groundwater was observed at an O3 dose of 10.0 mg/L and a contact time of 15 min, by 400 and 197%, respectively. On the other hand, for surface water, it was shown that after the ozonation/UV process, the AOC and BDOC content decreased after both 10 and 30 min of radiation in comparison to the water after ozonation. The AOC content decreased by 33% and 22%, respectively, and the BDOC content by 27% and 31%, respectively. The results obtained in this study provide new information on the effect of different ozonation conditions and the combined method on the level of biodegradable organic fraction of water. It is recommended that BDOC and AOC should be monitored in Poland as routine indicators during the preparation of drinking water.

Biochar addition to compost heat recovery systems improves heat conversion yields

Introduction: Compost heat recovery systems (CHRS) represent an emerging technology to recover residual woody biomass from agroforestry and forestry activities and use the heat that is naturally produced during aerobic biodegradation (composting). However, a low oxygen concentration in the gas phase and self-drying and compaction of the compost body often limit efficient oxidation by microbial communities. Woodchip-derived biochar has often been proposed as a bulking agent and improver of water retention and of oxygen accessibility in the composting process, but the literature reporting its effects in the CHRS is scarce.Methods: Here, biochar (average particle size of 10 mm) was added at 10% (on weight basis) to chipped pruning residues into two bench-scale-controlled reactors (0.2 m3), operated in parallel for 57 days.Results and Discussion: The addition of 10% (w/w) biochar to the composting body increased biodegradation yields by approximately 50% and improved oxidation rates over readily biodegradable organic fractions (addition of cheese whey). Temperatures were on average 1.34°C higher, and heat extraction flux was also improved in the presence of biochar (0.3 kW/m3) versus in its absence (0.1 kW/m3). The organic matter mass balance resulted in approximately 50% higher biodegradation yield and improved oxidation rates over readily biodegradable organic fractions. Microbial analysis highlighted a higher concentration of thermophilic species and a lower concentration of well-known pathogenic and antibiotic-resistant genera in the presence of biochar.

Accelerating Biodegradation: Enhancing Poly(lactic acid) Breakdown at Mesophilic Environmental Conditions with Biostimulants.

Poly(lactic acid) (PLA) has garnered interest due to its low environmental footprint and ability to replace conventional polymers and be disposed of in industrial composting environments. Although PLA is compostable when subjected to a suitable set of conditions, its broader acceptance in industrial composting facilities has been affected adversely due to longer degradation timeframes than the readily biodegradable organic waste fraction. PLA must be fully exposed to thermophilic conditions for prolonged periods to biodegrade, which has restricted its adoption and hindered its acceptance in industrial composting facilities, negating its home composting potential. Thus, enhancing PLA biodegradation is crucial to expand its acceptance. PLA's biodegradability is investigated in a compost matrix under mesophilic conditions at 37 °C for 180 days by biostimulating the compost environment with skim milk, gelatin, and ethyl lactate to enhance the different stages of PLA biodegradation. The evolved CO2, number average molecular weight (Mn), and crystallinity evolution are tracked. To achieve a Mn ≲ 10kDa for PLA, the biodegradation rate is accelerated by 15% by adding skim milk, 25% by adding gelatin, and 22% by adding ethyl lactate. This work shows potential techniques to help biodegrade PLA in home composting setting by adding biostimulants.

Removal of dissolved organic matter via a combination of UV/persulfate oxidation and biological activated carbon (BAC) process

The combination process of UV/peroxydisulfate (UV/PDS) oxidation and BAC (UV/PDS-BAC) is examined as an advanced treatment for conventional drinking water treatment through a continuous flow experimental apparatus with actual sand filter effluent as feed water. The effect of three key parameters (UV dosage, PDS dosage, and water temperature) on dissolved organic matter (DOM) is evaluated via long-term monitoring data. The removal behavior of DOM in the UV/PDS-BAC process is also compared with that in the O3-BAC process. Further, this study delves into the mechanisms behind the enhanced DOM removal achieved by UV/PDS-BAC. The achieved results reveal that impressive removal rates of 42.1 % for total organic carbon (TOC) and 62.9 % for UV254 could be achieved in the presence of specific conditions: a UV input of 200 mJ/cm2 and a PDS dosage of 0.5 mM. The efficiency of DOM removal by UV/PDS-BAC exhibits a positive correlation with the UV dosage ranging from 50 to 200 mJ/cm2, and the water temperature in the range of 4–30 ℃. While increasing the PDS dosage from 0.01 to 0.5 mM results in improved DOM removal, any further increase yields reduced removal efficiency. In comparison, subjected to similar operating conditions, UV/PDS-BAC outperforms O3-BAC in removing DOM. The superior DOM removal achieved by the UV/PDS-BAC process can be essentially attributed to several factors: firstly, it exhibits excellent mineralization capacity for low molecular weight organics (<1 kDa) and leads to increased biodegradable fraction of organics by oxidatively degrading DOM with a molecular weight >3 kDa. Secondly, the appropriate exposure of activated carbon pores is chiefly maintained by suitably controlling the biomass. Lastly, the enhanced biodegradability of BAC influent stimulated the growth of bacteria proficient in degrading DOM.

Effect of FeCl3 concentration in chemically enhanced primary treatment on the performance of a conventional wastewater treatment plant. A case study

The effect of coagulant dosage in a chemically enhanced primary treatment (CEPT) on the performance of a conventional wastewater treatment plant (WWTP) has been investigated. Lab-scale experiments simulations were carried out in order to evaluate the effect of coagulant addition on the primary settling performance. In these experiments, FeCl3 was used as coagulant. Later, the WWTP was theoretically simulated using a commercial software (WEST®) to evaluate the effect of coagulation/flocculation on the global system, based on the results obtained at lab-scale. According to these results, the CEPT modifies the organic matter balance in the WWTP, decreasing the contribution of readily (SS) and slowly (XS) biodegradable fractions of COD to the aerobic biological process up to 27.3% and 80.8%, respectively, for a dosage of FeCl3 of 24 mg L−1. Consequently, total suspended solids in the aerobic reactor and the secondary purged sludge decreased up to 33% and 13%, respectively. However, the influence on effluent quality was negligible. On the contrary, suspended solids concentration in the sludge to be treated by anaerobic digestion increased, mainly regarding the Ss and Xs fractions, which caused an 8.1% increase in biogas production potential, with approximately 60% of CH4 concentration.

Assessment of the Feasibility of Converting the Liquid Fraction Separated from Fruit and Vegetable Waste in a UASB Digester.

Anaerobic digestion of food waste still faces important challenges despite its world-wide application. An important fraction of food waste is composed of organic material having a low hydrolysis rate and which is often not degraded in digesters. The addition of this less hydrolysable fraction into anaerobic digesters requires a longer hydraulic residence time, and therefore leads to oversizing of the digesters. To overcome this problem, the conversion of the highly biodegradable liquid fraction from fruit and vegetable waste in a up-flow anaerobic sludge blanket (UASB) digester is proposed and demonstrated. The more easily biodegradable fraction of the waste is concentrated in the liquid phase using a 2-stage screw press separation. Then, this liquid fraction is digested in a 3.5 L UASB digester at a high organic loading rate. A good and stable performance was observed up to an organic loading rate (OLR) of 12 g COD/(Lrx.d), with a specific methane production of 2.6 L CH4/(Lrx.d) and a degradation of 85% of the initial total COD. Compared to the conversion of the same initial waste with a continuously stirred tank reactor (CSTR), this new treatment strategy leads to 10% lower COD degradation, but can produce the same amount of methane with a digester that is twice as small. The scale-up of this process could contribute to reduced costs related to the anaerobic digestion of food waste, while reducing management efforts associated with digestate handling and increasing process stability at high organic loading rates.

Automated Waste Segregation into Biodegradable and Non-Biodegradable Fractions Utilizing Conveyor Belt and Image Processing with Design Thinking

This research aims to propose a new strategy for the classification of waste through a joint venture. Conveyor system and image processing technology capabilities. The combination of these technologies can separate waste into biodegradable and non-biodegradable parts, thereby simplifying waste management, reducing pollution, and promoting better practices. In recent years, image processing technology has made significant progress in machine learning algorithms, object recognition, and classification based on visual features. By applying these advances to waste classification, biodegradable and non-biodegradable materials can be instantly identified even in diverse and fast-moving wastewater. The conveyor system provides a dynamic platform for the continuous transportation of waste, allowing the separation process to operate efficiently. This research contributes to ongoing efforts to promote sustainable waste management, reduce waste generation, and improve recycling rates. The automatic waste separation system proposed in this paper provides practical and efficient waste separation facilities and can be customized to meet the specific needs of waste management facilities. The integration of conveyor belts and image processing equipment is promising for future advances in waste distribution and environmental protection. As the world's interest in environmental sustainability and waste management continues to grow, new solutions to improve the disposal process are vital. This work presents an integrated system using conveyor belts and image processing equipment to separate waste into biodegradable and non-biodegradable materials. The main purpose of the system is to facilitate waste management, reduce pollution, and improve recycling.

Assessing the chronic toxicity of spreading organic amendments on agricultural soil: Tests on earthworms and plants

Recycling organic wastes on agricultural soils improves the soil quality, but the environmental and health impact of these organic amendments closely depends on their origins, their bio-physicochemical characteristics and the considered organisms potentially affected. The aim of this study was to assess the potential chronic ecotoxicity of spreading organic amendments on agricultural soils. To do this, we characterized three different organic amendments: sewage sludge from an urban wastewater treatment plant, cow manure and liquid dairy manure. Their chronic ecotoxicity was studied through assays exposing earthworms of the species Eisenia fetida and two plants: Medicago sativa and Sinapis alba. Of the three amendments, the sewage sludge presented the highest concentrations of micropollutants and a considerable fraction of available and biodegradable organic matter. The cow manure and liquid dairy manure had lower chemical contamination and similar characteristics with lower biodegradable fractions of their organic matter. No chronic phytotoxicity was evidenced: on the contrary, particularly with sewage sludge, the germination rate and aerial and root biomass of the two plants increased. Considering earthworms, their biomass increased considerably during the reproduction assays in soil amended with sewage sludge, which contained the more bioavailable organic matter. Nonetheless, the earthworms presented an inhibition close to 78% of the production of juveniles when exposed to sewage sludge exceeding 20 g.kg-1 DW (that means 2 times the agronomic dose). This reprotoxic effect was also observed in the presence of liquid dairy manure, but not with cow manure. At the end of the assays, the glycogen and protein reserves in earthworms exposed to sewage sludge were inferior to that of control earthworms, respectively around 50% and 30%. For the earthworms exposed to liquid dairy manure, protein and lipid reserves increased. In the case of liquid dairy manure, this reprotoxic effect did not appear to be linked to the presence of micropollutants. In conclusion, our results confirm the need to use several ecotoxicity assays at different biological levels and with different biological models to assess the ecotoxic impacts of soil amendments. Indeed, although certain organic wastes present a strong nutritional potential for both plants and earthworms, a not inconsiderable risk was apparent for the reproduction of the latter. An integrated ecotoxicity criterion that takes into account a weighted sum of the different results would guide the utilization of organic amendments while ensuring the good health of agricultural ecosystems.

Analysis of particle size distribution of organic carbon for landfill leachate—implications for sustainable treatment

AbstractBACKGROUNDLandfill leachate has a complex composition requiring experimental support to formulate a sustainable treatment strategy. This study utilized the particle size distribution (PSD) of the chemical oxygen demand (COD) content to assess the profile of biodegradable and inert COD fractions; it also emphasized the functions and benefits of ultrafiltration and nanofiltration modules coupled to an activated sludge process. The evaluation profited from the field data of a landfill site in Istanbul, where the leachate was actually treated in a membrane bioreactor (MBR) plant.RESULTSCOD and total nitrogen levels fluctuated between 10 100–31 200 mg L−1 and 1150–2800 mg L−1, respectively. PSD analysis for COD, conducted at two extremes, displayed similar results, where the majority of the COD was observed to accumulate at the low extremity of the particle size, 70–72% below 2 nm. Therefore, direct membrane filtration of leachate yielded low COD removals that were limited to 9% with ultrafiltration and to 31–35% with nanofiltration. COD fractionation indicated a ratio of 5% for the inert COD in leachate. The permeate COD of ultrafiltration in the existing plant was 2000 mg L−1, much higher than the inert fraction ratio, which was further reduced to 266 mg L−1 by nanofiltration.CONCLUSIONPSD analysis was an integral complement of respirometry for establishing the size‐biodegradation relationships of different COD fractions. It located the majority of soluble COD fractions below 0.55 nm, thus implying the necessity of a biological process. PSD also identified the generation of soluble residual metabolic products, indicating that residual COD escaping treatment would be equally significant to the removal potential of the biodegradable substrate. © 2023 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry (SCI).

Restructuring anaerobic hydrolysis kinetics in plant-wide models for accurate prediction of biogas production

In this study, the effect of anaerobic hydrolysis rate on biogas production was investigated with mesophilic digesters in seven large-scale wastewater treatment plants. A linear correlation was determined between the percentage of primary sludge mass in the total sludge fed to the digester and the overall anaerobic hydrolysis rate. The anaerobic hydrolysis rate of primary sludge was determined to be three times higher than that of biological sludge. The reduction factors for anaerobic hydrolysis (ηHYD,ana) were identified in the range of 0.11–0.30 which is lower compared to the recommended range (0.30–0.50) given in the literature. This study proposes a new model approach where anaerobic degradation kinetics of influent originated (XB) and decay originated (XB,E) particulate biodegradable organics are separated. Current plant-wide models with a single kinetic expression required recalibration of the model for calculating biogas flowrate for each treatment facility with different primary and secondary sludge ratios fed to the digesters. The new model structure is able to predict biogas production of all wastewater treatment plants without any recalibration effort by segregating degradation kinetics of two particulate biodegradable organic fractions (XB, XB,E).

Sustainable Recovery of Urban Organic Waste: A Case Study of Composting Waste from the Mfidi Market in Kinshasa, Democratic Republic of the Congo

Aims: This study aimed to evaluate the methods of solid waste management in the Mfidi market in order to detect the various problems and propose strategies to fight against insalubrity.&#x0D; Study Design: This research consists of four parts, an introduction with literature review, a description of the study environment, the methods used and finally the results obtained.&#x0D; Location and Duration of the Study: This study took place in the Mfidi market in Kinshasa from January 20 to May 15, 2023.&#x0D; Methodology: The methodological approach was based on observation and experimentation. The quantification consisted in characterizing the waste, recovering the biodegradable organic fraction by the technique of composting. Then test the effectiveness of this compost in its application in the field.&#x0D; Results: This study showed that the quantity of waste at the Mfidi market varies daily between 1,580 and 4,827 kg, with an average of 3,543.8 kg. With regard to the characteristics of the waste, we note that plants and others are the most important with an average proportion of 59.5%, followed respectively by cardboard and paper (16.6%), plastics (14.6 %), metals (5.4%), textiles (2.1%) and glass (1.9%). The organic fraction was recycled into compost, whose contribution to soil fertility was justified by the growth of the species Amaranthus hubridus sp.&#x0D; Conclusion: The establishment of an integrated solid waste management system within the Mfidi market can guarantee sanitation conditions in this market.

Development of conversion factors to estimate the concentrations of heavy metals in manure-derived digestates

During biogas production, a residual by-product rich in organic matter, nutrients, and trace elements - called digestate - is generated. Due to the nature of the anaerobic digestion process (i.e., conversion of organic matter into biogas) and the non-digestibility of trace elements, metal concentrations are higher in digestate than initially in the treated feedstock, resulting in a detrimental effect on the environment when directly applied as fertiliser on the soil. This study aims to predict the concentration of heavy metals in digestate through four different process parameters (Biogas yield - M1, Biodegradable fraction - M2, Dry matter - M3 and Power generation - M4) in full-scale biogas plants. For the validation of the process parameters, the predictions were compared against laboratory analyses of feedstocks and digestates samples from mono- and co-digestion processes. The convergence between the conversion factors based on laboratory data and process parameters (CLD and CFA, respectively) ranged in the following order: M3 >M2 >M1 > M4. Based on laboratory analyses, better predictions were obtained for Al, Cr, Cu, Fe, Mn, and Zn employing M3. Moreover, a robust convergence was achieved between the CLD and CFA conversion factors for the mono-digestion process. Further assessment of a diverse range of feedstocks is needed to increase the convergence between the conversion factors based on process parameters and laboratory data, specifically for the co-digestion process M3. The concentrations of Cd, Co, Ni, and Pb elements were below the detection limits, whereas Cr, Cu, and Zn did not exceed the legal threshold limits of the legislations.