This study explores the development of advanced wound dressings by enhancing traditional bandages with suitable biomedical materials. We utilized Hyaluronic Acid (HA) and Polyvinyl Alcohol (PVA) as a polymeric matrix to create a novel membrane. Employing a film casting technique, we incorporated varying ratios of biological nanoparticles—Hydroxyapatite (HAP) and Cerium Vanadate (Ce₃(VO₄)₄)—to improve the wound dressing's properties. The resulting films were characterized using several techniques, including X-ray Diffraction (XRD), Fourier Transform Infrared (FT-IR) spectroscopy, Raman spectroscopy, and Thermogravimetric Analysis (TGA). Wettability assessments provided insights into the surface characteristics of the scaffolds, revealing a diverse range of contact angles that reflect varying hydrophilic properties. Notably, the pure PVA/HA sample exhibited a contact angle of approximately 31.2° ± 12.1°, indicating excellent hydrophilicity and a strong capacity for drug absorption. However, the incorporation of nanoparticles resulted in an increase in contact angles, suggesting a slight reduction in hydrophilicity. Specifically, the addition of HAP produced a contact angle of about 36.5° ± 6.7°, while the addition of Ce₃(VO₄)₄ resulted in a contact angle of approximately 38.6° ± 6.4°. A significant increase in contact angle to 42.23° ± 4.8° was observed when mixing the nanoparticles, though the introduction of Graphene Oxide (GO) reduced the angle to 37.4° ± 0.35°, enhancing the hydrophilic properties of the composite. Furthermore, in vitro cell viability testing was conducted to confirm the biocompatibility and bioactivity of the fabricated scaffolds. The results demonstrated the ability of the scaffolds to promote cell growth, with an increase of 88.4% in cell growth ratio observed at a suitable drug concentration of approximately 0.68 μg/ml. These findings highlight the potential of the developed scaffolds to enhance the wound healing process.