Biodiversity loss caused by housing is not a well-defined sector of environmental impact. This research quantifies effects on biodiversity of an average Spanish Single-Family House (SFH) with 180 m2 of built surface. The current Spanish SFH stock GWP amounts to 1.16 Gt CO2eq in a 50-year life cycle, 40 % of which is embodied in the building materials and the 60 % are emissions due to the use of the building. This stock also impacts with 10.2 Gt 1,4-DCB the land, water and human health. SFHs also drive 6052 species extinct in a 50 year life cycle, and account for 3.03 M years of life lost due to premature death or lived with a disability. Divided by the 16 M people living in Spanish SFHs, each one lost 0.19 years of their lives (68.1 days) due to their home's impacts on human health.The article compares a reference conventional building against three low-impact cases, to understand how different building techniques and materials influence environmental outcomes that keep biodiversity loss the lowest possible. Scenarios include a standard brick and concrete house as Scenario 0 (SC0, Base), a timber Passivhaus as Scenario 1 (SC1), a straw-bale house with renewable energies as Scenario 2 (SC2), and an earth bioclimatic house as Scenario 3 (SC3). An initial Global Warming Potential (GWP) analysis was performed to relate previous building Life Cycle Assessment (LCA) studies with biodiversity metrics. Three main biodiversity metrics; ecotoxicity (as midpoint indicator), biodiversity loss and damage to human health (both as endpoint indicators) have been considered.Compared to SC0 with 1292 kgCO2-eq·m−2 (516 embodied) of GWP, we found that SC1 emitted −47.0 % of that, SC2–41.4 % and SC3–80.9 %. Concerning ecotoxicity, where SC0 has 11,399 kg 1,4 DCB, the results are −27.9 % in SC1, −19.2 % in SC2, and −45.6 % in SC3. Regarding biodiversity loss, where SC0 has 7.54 E−06 species.yr·m−2, the impacts are −30.9 % in SC1, −32.6 % in SC2, and −58.6 % in SC3.Human health damage in SC0 being 3.37 E−03 DALY, has been reduced in the timber home (SC1) is −44.2 %, of the Straw SFH (SC2) −39.2 %, and of the earth house (SC3) −67.1 %.This article shows that with current existing technological solutions GWP could be reduced in −80.9 %, ecotoxicity in −45.6 %, biodiversity loss in −58.6 % and human health in −67.1 %. Spanish Single-Family Houses built in timber, earth or straw-bale are real alternatives to current cement traditional building.