Lambda cyhalothrin (LCT), a prominent pyrethroid insecticide, has been linked to a number of negative side effects in non-target animals, including mammals. This study investigated the long-term consequences of lambda- cyhalothrin (LCT) exposure on albino rats, including physiological, biochemical, and behavioural alterations. Adult albino rats were given sub-lethal lambda-cyhalothrin (LCT) doses over an extended period to simulate chronic exposure. Body weight, organ histology, serum biochemical markers, and behavioural reactions were all examined to determine the toxicological impact. The biochemical markers of liver and kidney function showed substantial variation, indicating organ damage. Histopathological evaluation of the liver and kidney tissues revealed structural damage consistent with the biochemical abnormalities discovered. The neurotoxic potential of lambda-cyhalothrin (LCT) was further demonstrated by the exposed rats' notable behavioural changes, which included decreased locomotor activity and increased anxiety-like behaviour. These findings emphasise the potential long-term risks associated with repeated exposure to lambda cyhalothrin (LCT), emphasizing the need for additional research and strong regulations to understand the implications for environmental safety and public health.