The efficiency of nitrifying bacteria bio-augmentation into biofilm microbiocenosis during 30-day continuous biofiltration of municipal wastewater model solution has been assessed. The laboratory setup consisted of two parallel operating biofilters, in one of which, after start-up period, cultures of ammonium-oxidizing and nitrite-oxidizing bacteria of the Nitrobacter genus were sequentially introduced. It was established that the bio-augmentation of ammonium-oxidizing bacteria into the biofilm microbiocenosis led to an increase in the efficiency of ammonium nitrogen removal by an average of 1.6 times compared to the control biofilter. The subsequent bio-augmentation of nitrite-oxidizing bacteria caused an increase in the amount of nitrates in purified water by 2 times on average. As a result of bio-augmentation of nitrifying bacteria into the biofilm microbiocenosis, the nitrification process was intensified. Quantitative and qualitative identification of microorganisms via fluorescence in situ hybridization showed an increase in the number of nitrifying microorganisms in the biofilm of experimental biofilter, which confirms the efficiency of introduction of microorganisms and correlates with the data on biotransformation of nitrogen compounds. nitrifying microorganisms, wastewater biofiltration, biofilms, bio-augmentation, fluorescence in situ hybridization.