The partition is a boundary that can effectively suppress the spread of bioaerosols. It has been widely used in public places such as restaurants and shopping malls during COVID-19 pandemic. Inspired by this, this study explored the effect of partial partitions in limiting the diffusion of bioaerosols in a typical isolation ward. In bioaerosol experiments, Serratia marcescens was used as bioaerosol releasing material to study the bioaerosol distribution. Simultaneously, the result of bioaerosol sampling was used to validate the numerical model. Furthermore, the effect of five partitions strategies on aerosol diffusion was further explore by Computational fluid dynamics. The flow patterns in the ward and the spatiotemporal distribution of aerosols were obtained and analyzed. The results showed that the best strategy could increase bioaerosol removal effectiveness index from 0.569 to 0.696, which accelerated the bioaerosol removal. Nevertheless, only selecting a reasonable size and installation position, can the partial partition play a positive role in the interruption of aerosol diffusion. Moreover, small-area partial partition can achieve efficient control of bioaerosol diffusion as well.