Identification Of Bioactive Compounds Research Articles

Rhizophora mucronata Lam. (Mangrove) Bark Extract Reduces Ethanol-Induced Liver Cell Death and Oxidative Stress in Swiss Albino Mice: In Vivo and In Silico Studies.

The bark extract of Rhizophora mucronata (BERM) was recently reported for its prominent in vitro protective effects against liver cell line toxicity caused by various toxicants, including ethanol. Here, we aimed to verify the in vivo hepatoprotective effects of BERM against ethanol intoxication with the prediction of potential targets employing in silico studies. An oral administration of different concentrations (100, 200 and 400 mg/kg body weight) of BERM before high-dose ethanol via intraperitoneal injection was performed in mice. On day 7, liver sections were dissected for histopathological examination. The ethanol intoxication caused liver injury and large areas of necrosis. The pre-BERM administration decreased the ethanol-induced liver damage marker tumor necrosis factor-alpha (TNF-α) expression, reduced hepatotoxicity revealed by nuclear deoxyribonucleic acid (DNA) fragmentation and decreased oxidative stress indicated by malondialdehyde and glutathione contents. Our in silico studies have identified BERM-derived metabolites exhibiting the highest predicted antioxidant and free radical scavenger activities. Molecular docking studies showed that most of the metabolites were predicted to be enzyme inhibitors such as carbonic anhydrase inhibitors, which were reported to stimulate the antioxidant defense system. The metabolites predominantly presented acceptable pharmacokinetics and safety profiles, suggesting them as promising new antioxidant agents. Altogether, the BERM extract exerts antioxidative activities and shows promising hepatoprotective effects against ethanol intoxication. Identification of related bioactive compounds will be of interest for future use at physiological concentrations in ethanol-intoxicated individuals.

Comprehensive Assessment of Houttuynia cordata Thunb., an Important Medicinal Plant and Vegetable

Houttuynia cordata Thunb., called Yuxingcao in Chinese, is an important medicinal plant and vegetable consumed in the southern regions of China. This review aims to summarize studies on the phyto-physiological chemistry, cytology, molecular biology, and genomics of H. cordata. Studies on the physiology and biochemistry of H. cordata have grown over the past few decades. Phenotypic and agronomic traits, tissue culture, elemental analysis, photosynthetic studies, bioactive compound identification, and antioxidant research have been reported. Molecular biological studies, such as those of molecular markers, microRNAs, DNA variations, protein variations, and transcriptomes have also advanced. Recent studies have focused on the rDNA and chloroplast genome of this plant. This review could serve as a basis to perform the genetic breeding, genomic advance, and cultivation of this valuable diversified plant resource for medicinal applications and vegetable production.

Methods for Rapid Screening of Biologically Active Compounds Present in Plant-Based Extracts.

In recent years, there has been an increased interest in products of natural origin. The extraction procedure of bioactive compounds from plant matrices is a crucial step in the development of useful new bioproducts for everyday life. The utilisation of analyses enabling the rapid identification of the presence of a given group of compounds can be helpful in the early stages of the development of new products in order to save time and reduce costs. Within this article, we have presented a comparison of different, accessible methods for the identification of various bioactive compounds, e.g., saponins, carboxylic acids, oils and fats, proteins and amino acids, steroids, and alkaloids in plant-based extracts. Additionally, the multielemental composition of extracts was also examined. The applied methods allowed for confirmation of the presence of biologically active compounds in bio-products obtained by ultrasound-assisted extraction. At a later stage, these procedures should be supplemented by advanced analytical techniques in order to determine the plant chemicals’ content qualitatively and quantitatively.

In-silico screening and identification of potential bioactive compounds of Trifolium repens against pathogenic bacterial target proteins

From medicinal plants, a number of bioactive substances have been discovered and reported in the past, including flavonoids, alkaloids, phenols, tannins, saponins, and sterols. To identify the most effective natural bioactive compounds against the target proteins of bacteria, the bioactive compounds from T. repens have been studied in the current work. Numerous infections like chronic sinusitis, otitis media, intravascular catheter infection, pacemaker infection, peritoneal dialysis, urinary infection, prosthetic joint infection, pulmonary infection, and keratitis are caused by biofilm-forming bacteria. The increased rate of biofilm formation has threatened life forms as they show resistance to most antibiotics. During in-silico analysis, among the nine bioactive compounds listed, two bioactive compounds namely Trifolin and Naringenin were selected as their biological activity has not been reported. The optimum binding affinities between ligand–protein were chosen after screening study of 22 target proteins from Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterococcus faecalis, and Klebsiella pneumoniae. The effects of trifolin and naringenin, two naturally occurring bioactive chemicals from T. repens, on several bacterial biofilm target proteins were investigated. An in-silico study was carried out to determine the interaction between the ligand and receptor (target proteins). The top docking poses showed multiple conventional hydrogen bond, which indicated a high binding affinity with the bacterial target biofilm proteins.

Extraction, isolation and identification of bioactive compound from extract of Euphorbia thymifolia Linn

Natural products are stored in plants, animals, and microorganisms, which are referred to as "natural sources generating compounds." Strong therapeutic substances are developed with the help of medicinal plants. As a source of several beneficial secondary metabolites that work as a plant's defensive system against predators including microorganisms, insects, and herbivores and have been shown to be potentially active chemicals, plant-based medicines provide a significant addition to current treatments. Common names for Euphorbia Thymifolia Linn (E. Thymifolia) include "duddi" and "Laghu didhika" and "Raktavindaka," respectively, in Sanskrit. It is a member of the Euphorbiceae family. This plant is used as a diuretic, laxative, and thermogenic. It is bitter, acrid, and sweet. This plant is commonly used in ayurveda to treat a variety of illnesses, including leprosy, helminthiasis, and ringworm skin infections. The current study's objective is to analyze the entire E. thymifolia Linn plant in order to isolate and identify bioactive components and determine its phytochemical profile. An established test technique that is documented in the literature was used to determine the qualitative analysis of different phytochemical elements. From a methanolic extract of E. thymifolia, a bioactive component was isolated and characterized.

Inhibition of methanol extract snail gonggong (Strombus urturella) to pathogenic bacteria Vibrio alginolyticus

The purpose of this study was to determine the methanol extract of snail gonggong (Strombus turturella) and the inhibition of methanol extract of gonggong snail meat (Strombus turturella) against the pathogenic bacterium Vibrio alginolyticus. The study used a completely randomized design. The treatment was giving the extract of snail gonggong meat with concentration of 100%, 50%, 25%, positive control using amoxcillin and negative control using distilled water with three replications. The identification of bioactive compounds included alkaloids, saponins, flavonoids, steroids and triterpenoids. The antibacterial activity of the methanol extract of snail gonggong meat (Strombus turturella) was tested by the diffusion method at concentrations of 100%, 50% and 25%. The data collected is then analyzed descriptively qualitatively. The results showed that the antibacterial activity of the 100% concentration of the methanol extract of the gonggong snail meat had moderate inhibition against Vibrio alginolyticus (diameter 7,833 mm). while the concentrations of 50% and 25% did not have an inhibition zone. The identified bioactive compounds included alkaloids, saponins, flavonoids and steroids, while the terpenoid and phenolic compounds were not identified. Thus, the methanol extract of gonggong snail meat has antibacterial potential that can be used to overcome the attack of pathogenic bacteria.

Isolation, characterization and identification of bioactive compounds from Herbal Medicines and Medicinal Plant Extracts by Fourier Transform Infrared Spectroscopy: A brief review

Herbal medicines are mostly used from thousands of years in primary health care of society and community of both developed and developing countries. It mainly includes whole plant, herbs, extracts, isolated compounds, polyherbal preparations, phytoformulations etc. Identification of bioactive compounds from phytomedicines plays a very important role in drug development and drug design process. The characterization of phytomedicines is very much essential in the identification of compounds. For the purpose of identification two methods, Spectroscopy and Chromatography play important role. Fourier Transform Infrared Spectroscopy (FTIR) is the widely used technique which is mainly used to identify functional groups in herbal medicines. Various scientific research articles published on extraction, isolation, characterization and identification of phytoconstituents from medicinal plants and other forms of herbal medicines is being reviewed in this article. We have mainly focused on the FTIR characterization and identification of phytochemicals from herbal medicines and are briefly presented in this review work. Our present review article concludes that the FTIR spectroscopy plays very much essential role in the identification and characterization of bioactive compounds from herbal medicines.

Comprehensive Lichenometabolomic Exploration of Ramalina conduplicans Vain Using UPLC-Q-ToF-MS/MS: An Identification of Free Radical Scavenging and Anti-Hyperglycemic Constituents.

In this study, we propose ultra-performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-QToF-MS/MS)-guided metabolite isolation as a choice analytical approach to the ongoing structure–activity investigations of chemical isolates from the edible lichen, Ramalina conduplicans Vain. This strategy led to the isolation and identification of a new depside (5) along with 13 known compounds (1–4, 6–14), most of which being newly described in this lichen species. The structures of the isolates were established by detailed analysis of their spectral data (IR, NMR, and Mass). The acetone extract was further analyzed by UPLC-Q-ToF-MS/MS in a negative ionization mode, which facilitated the identification and confirmation of 18 compounds based on their fragmentation patterns. The antioxidant capacities of the lichen acetone extract (AE) and isolates were measured by tracking DPPH and ABTS free radical scavenging activities. Most isolates displayed marked radical scavenging activities against ABTS while moderate activities were observed against DPPH radical scavenging. Except for atranol (14), oxidative DNA damage was limited by all the tested compounds, with a marked protection for the novel isolated compound (5), as previously noted for the acetone extract (p < 0.001). Furthermore, compound (4) and acetone extract (AE) have inhibited intestinal α-glucosidase enzyme significantly (p < 0.01). Although some phytochemical studies were already performed on this lichen, this study provided new insights into the isolation and identification of bioactive compounds, illustrating interest in future novel analytical techniques.

Artemisia vulgaris inhibits BCR/ABL and promotes apoptosis in chronic myeloid leukemia cells.

In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. The aim of the present study was to determine and clarify the mechanism of action of AVM. BCR/ABL activation is present in >90% of CML cases. As a result, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 (human wild-type) or TCCY-T315I (human imatinib-resistant) and the Ba/F3-(T315I/E279K/Y253H) (mouse BCR/ABL point mutation-transfected cells). The results revealed that AVM inhibited the phosphorylation of BCR/ABL and their subsequent molecular signals including AKT and MAPK activation. AVM induced the release of cleaved PARP and cleaved caspase-3 caused apoptosis and inhibited the viability of these cells. Interestingly, AVM appeared to be more sensitive to imatinib-resistant (T315I, Y253H, and E279K) than wild-type BCR/ABL cells, indicating its potential to overcome imatinib-resistant severe issues in CML. Moreover, the effects of various sub-fractions of AVM were then investigated in order to determine the optimal solvent for the identification of anticancer bioactive compounds. The results demonstrated that the ethyl acetate and chloroform fractions were more effective than the n-hexane and water fractions. It is thus concluded that AVM inhibits the activity of BCR/ABL and their subsequent molecular signals, including AKT and MAPK, resulting in cytotoxicity via apoptosis.

Determining Toxic Potencies of Water-Soluble Contaminants in Wastewater Influents and Effluent Using Gene Expression Profiling in C. elegans as a Bioanalytical Tool

With chemical analysis, it is impossible to qualify and quantify the toxic potency of especially hydrophilic bioactive contaminants. In this study, we applied the nematode C. elegans as a model organism for detecting the toxic potency of whole influent wastewater samples. Gene expression in the nematode was used as bioanalytical tool to reveal the presence, type and potency of molecular pathways induced by 24-h exposure to wastewater from a hospital (H), nursing home (N), community (C), and influent (I) and treated effluent (E) from a local wastewater treatment plant. Exposure to influent water significantly altered expression of 464 genes, while only two genes were differentially expressed in nematodes treated with effluent. This indicates a significant decrease in bioactive pollutant-load after wastewater treatment. Surface water receiving the effluent did not induce any genes in exposed nematodes. A subset of 209 genes was differentially expressed in all untreated wastewaters, including cytochromes P450 and C-type lectins related to the nematode’s xenobiotic metabolism and immune response, respectively. Different subsets of genes responded to particular waste streams making them candidates to fingerprint-specific wastewater sources. This study shows that gene expression profiling in C. elegans can be used for mechanism-based identification of hydrophilic bioactive compounds and fingerprinting of specific wastewaters. More comprehensive than with chemical analysis, it can demonstrate the effective overall removal of bioactive compounds through wastewater treatment. This bioanalytical tool can also be applied in the process of identification of the bioactive compounds via a process of toxicity identification evaluation.Graphical abstract

Bioactive Compounds Identification, Antioxidant and Antibacterial Activities of Algerian Marine Algae Extracts

Background: Marine algae produce bioactive compounds, including polyphenols. They have antioxidant, antibacterial, immune regulation and other biological activities. The aim of this study was to examine the antibacterial and antioxidant activities of extracts of five marine algae species: Cystoseira humilis, Halopteris scoparia, Pavonica padina, Sargassum vulgare and Rhodomela confervoides of Bejaia’s coast (northern Algeria). Methods: Phenolic content of the five marine algae was assessed using Folin Ciocalteu method and then characterized by HPLC-DAD and HPLC-ESI-QTOF-MS analyses. The antioxidant activity was evaluated by DPPH•, ABTS•+, NO• radical scavenging and FRAP assays. For the screening of the antibacterial activity, six strains were tested using three different methods: agar disc diffusion, agar well diffusion and spot methods. The minimum inhibitory concentrations (MICs) were also determined. Results: Protocatechuic acid was the phenolic compound identified in all the studied seaweeds, while Padina pavonica was the species where more phenolic compounds were identified (phloroglucinol, gallic acid, ferulic acid and syringic acid). Cystoseira humilis showed the highest antioxidant activity (DPPH•: 2237.50 ± 148.34 μM TE/100 g dw, ABTS•+: 1992.50 ± 165.97 μM TE/100 g dw, NO•: 2559.58 ± 42.08 μM TE/100 g dw, FRAP: 2081.20 ± 115.29 μM TE/100 g dw), which was also the richest in phenolic compounds (4.63 ± 0.23 mg PhlE/g dw). Rodomela confervoides showed an important activity against all the bacterial strains tested with the greatest diameter of inhibition zone (18 mm) against E. coli with a MIC equal to 20 mg/mL. The extract of Padina pavonica showed a moderate activity (13.5 mm) against P. aeruginosa with a MIC ≤ 10 mg/mL and a MBC &gt; 10 mg/mL. Conclusion: The studied marine algae showed antioxidant and antibacterial activities. They could be potentially used for applications in medicine, food production and the cosmetic industry.

Detection, isolation and identification of more bioactive compounds from Fusarium equiseti, an endophytic fungus isolated from Ocimum gratissimum

Detection, isolation and identification of more bioactive compounds from Fusarium equiseti, an endophytic fungus isolated from Ocimum gratissimum

Extraction, Identification and Quantification of Bioactive Compounds from Globe Artichoke (Cynara cardunculus var. scolymus)

This study looked at the best conditions for extracting bioactive compounds from globe artichoke (Cynara cardunculus var. scolymus), such as total phenolics, flavonoids, ascorbic acid, and inulin. The obtained results showed that the optimum conditions for extraction of total phenolics and flavonoids from receptacle and bracts of artichoke, when applying maceration at room temperature, with 70% methanol for 4 h. Genstin was the major phenolic compound (57.86 and 25.6 mg/100 g DM) in artichoke receptacle and bracts, respectively. The highest content of ascorbic acid extracted from artichoke parts was obtained using 1% citric acid solution at 25 °C. In addition, the optimum conditions for extraction of inulin were autoclave at 120 °C for 15 min. The most abundant essential amino acids were aromatic amino acids (phenylalanine and tyrosine), followed by valine, lysine, and leucine but sulfur amino acids were the limited amino acids found in the artichoke parts. Our results also suggested that bioactive compounds from artichoke extracts might have a promising future in the management of oxidative stress on the gastrointestinal tract and recommended as an attractive ingredient for developing functional food.

Detection, isolation and identification of more bioactive compounds from Fusarium equiseti, an endophytic isolated from Ocimum gratissimum

Endophytes have continued to gain fame due to their ability to produce an array of secondary metabolites within the host system with huge untapped pharmacological potentials. This study was carried out to further identify and isolate novel therapeutic compounds from Fusarium equiseti, an Endophytic fungus isolated from leaves of Ocimum gratissimum. Endophytic fungal isolation, fungal fermentation, and extraction of secondary metabolites were carried out using standard laboratory methods. The crude extracts of Fusarium equiseti were subjected to further chromatographic techniques using vacuum liquid chromatography, Sephadex LH 20 and semipreparative HPLC for isolation of bioactive compounds. The fractions and the isolated compounds obtained were further subjected to high performance liquid chromatography-Diode Array Detector (HPLC-DAD). The analytical HPLC led to the further detection of many bioactive compounds namely: Enniatin A, Aureonitol, Serasinoside H1, Altenusin, Aplysinamisin, benzylnitril, ruspolinone and Orientin. Semipreparative HPLC led to the isolation of 6 pure compounds of which two were identified as benzylnitril, and ruspolinone. The remaining four were not identified due to lack of library hits. The detected and the isolated compounds have been previously shown to exhibit a wide array of biological activities including antiviral, antifungal, hepatoprotection, antibacterial, anticancer, cytotoxic, and antioxidant properties. The unidentified compounds may hold enormous potential as new bioactive lead compounds for development into novel therapeutic agents. Therefore, the Endophytic fungus, Fusarium equiseti should be harnessed for its potential pharmacological, pharmaceutical, agricultural and industrial applications.

Anti-quorum sensing and cytotoxic activity of elemi essential oil

Essential oils have several biological activities such as antimicrobial, antioxidant, proliferative, and anti-inflammatory. This study aimed identification of bioactive compounds found in Elemi essential oil (EO) and to determine the anti-quorum sensing and cytotoxic activities of EO. In this study, bioactive compounds of EO were analyzed using GC-MS, and the antibacterial activity of elemi was screened against Staphylococcus aureus ATCC 25923, Methicillin-Resistant Staphylococcus aureus ATCC 43300, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa PAO1. Anti-biofilm activity and pyocyanin production on P. aeruginosa PAO1 were also investigated. The effect of EO on cell viability was also analyzed by thiazolyl blue tetrazolium bromide (MTT) and neutral red uptake (NR) assay in fibroblast cells. According to GC results, the major component of EO was determined as limonene (55%). A sub-MIC of elemi essential oil inhibited biofilm formation and pyocyanin production by 43% and 56%, respectively. On the other hand, EO also had an acute effect on the mitochondrial and lysosomal activities of fibroblast cell lines. Mitochondrial and lysosomal activities were significantly decreased when EO concentrations were applied for 24 and 48 hours (p&amp;lt;0.05). In conclusion, EO has inhibitory activity on biofilm formation and pyocyanin production, and also the lower doses of oil have no toxic effects on fibroblast cells. However, higher doses of EO have more cytotoxic effects on mitochondrial activity rather than the lysosomal activity of fibroblast cell lines. It is thought that EO exhibits these activities due to the amount of limonene in its content.

Insecticidal Potentials and Identification of Bioactive Compounds from Extracts of Bacopa Floribunda and Ludwigia Decurrens Plants

The negative impact of insecticides used to combat insect-borne plant ailments cannot be emphasized, as they have had a major influence on plant yields throughout time, and the side effect of insecticide residue on consumers cannot be overstated either. Callosobrochus maculatus is an insect that attacks cowpeas and has the potential to significantly reduce crop productivity. This research developed from investigations into the utilization of biological control technologies that have little or no harmful influence on humans or the environment. The leaves of Bacopa Floribunda (BFL) and Ludwigia Decurrens (LDL) were air dried, powdered, and extracted with n-hexane. The insecticidal activities were carried out according to protocol, and the LC50 was determined using online software from AAT Bioquest, Inc. The presence of bioactive compounds was detected using GC-MS. The Callosobrochus maculatus mortality rate was found to be a function of exposure time and concentration of the extracts, with the maximum mortality rate (66.67±8.82 percent) occurring after 72 hours at 0.5 mg/ml for BFL extract. The highest mortality rate (70.00 ±5.77 percent) was obtained for the LDL extract at 72 hours at 0.5 mg/ml of the extract concentration, which was better than the other extract (BFL) at 0.5 mg/ml. The lethal concentration LC50 for BFL varies from 0.251 (24 hours), 0.276 (48 hours), and 0.223 (72 hours), while the LC50 for LDL extract is 0.228 (24 hours), 0.039 (48 hours), and 0.663 (72 hours) (72 hrs). The presence of chemicals found could be linked to the extracts’ insecticidal properties. The excellent potential of the extracts as insect biocontrol agents against Callosobrochus maculatus can be recommended for future research as insect biocontrol agents.

Antioxidant and Hypoglycemic Activity of Sequentially Extracted Fractions from Pingguoli Pear Fermentation Broth and Identification of Bioactive Compounds.

Pear fruits have been reported to contain abundant bioactive compounds and exhibit antidiabetic activity. In this study, Pingguoli pear (Pyrus pyrifolia cv.‘Pingguoli’) fermentation broth was sequentially extracted by five solvents with increasing polarity (petroleum ether, chloroform, ethyl acetate, n-butanol, and water) to evaluate its antioxidant and hypothermic activities, and then the main compounds of the fraction with the highest activity were assessed, which might be responsible for such activities. The results showed that the ethyl acetate fraction (EAF) exhibited the highest antioxidant activity according to DPPH (IC50 = 0.238 mg/mL), ABTS (IC50 = 0.293 mg/mL), and FRAP (IC50 = 0.193 mg/mL) assays. The in vitro hypoglycemic activity assay showed that EAF exhibited the strongest inhibitory effect, with IC50 values of 0.34 and 0.95 mg/mL for α-amylase and α-glucosidase, respectively. The glucose consumption in HepG2 cells treated with EAF was significantly increased to 252%, compare with control group. Liquid chromatography–mass spectrometry analysis implied that the main compounds, 3′-C-glucosylisoliquiritigenin, robustside D, caffeic acid, and chlorogenic acid may be potential candidates for the antioxidant and hypoglycemic activities of the EAF. This study suggested that EAF of Pingguoli pear fermentation broth could be utilized for development of potential functional food and antidiabetic agents.

In Vitro Induction of Apoptosis in Isolated Acute Myeloid Leukemia Cells: The Role of Anastatica hierochuntica Methanolic Extract.

Anastatica hierochuntica L. (Cruciferae) has been known in Egyptian folk medicine as a remedy for gastrointestinal disorders, diabetes and heart diseases. Despite the wide usage, A. hierochuntica research provides insufficient data to support its traditional practice. The cytotoxicity of A. hierochuntica methanolic extract was investigated on acute myeloid leukemia blasts (AML) and normal human peripheral leucocytes (NHPL). The phytochemical identification of bioactive compounds using 1H-NMR and LC-ESI-MS was also performed. A. hierochuntica extract caused non-significant cytotoxicity on NHPL, while the cytotoxicity on AML was significant (IC50: 0.38 ± 0.02 μg/mL). The negative expression of p53, upregulation of Caspase-3 and increase in the BAX/BCL-2 ratio were reported at the protein and mRNA levels. The results suggest that A. hierochuntica extract induced AML cell death via the p53-independent mitochondrial intrinsic pathway and further attention should be paid to this plant as a promising natural anticancer agent.

Ceratonia siliqua L seeds extract: Experimental analysis and simulation study

The Ceratonia siliqua L seed extract was extracted using different extraction methods. The characterization and identification of bioactive compounds were carried out using the HPLC-DAD. The quantification of the polyphenol and flavonoid in the cold maceration and Soxhlet extract shows a high content of both phenolic compounds in the carob seed extract with the following values of 14, 713 mg A-G/g E, 19.25 mg A-G/g E of polyphenol content and of 1.92 mg-Q/g E and 2.47 mg-Q/g E of flavonoid content respectively. In similar, the antioxidant activity of the obtained extracts by the DPPH method shows a great capacity to trap the DPPH radical with an IC50 value of 0.041 and 0.014 mg/ml for maceration extract and Soxhlet respectively. The theoretical study using Monte Carlo simulation provides a good estimation of the interaction process between antioxidant agents and DPPH, these results are totally in accordance with data obtained experimentally.

Identification of Bioactive Compounds of Leaf Extracts of Sida cordata (Burm.f.) Borss.Waalk. by GC/MS Analysis.

Sida cordata (Burm.f.) Borss.Waalk. is a perennial prostrate herb belonging to the family Malvaceae found in the tropical and subtropical plains of India, Sri Lanka and Pakistan. It is one of the most essential plants in Ayurveda and Siddha system of traditional medicine and is used to treat various diseases and ailments. The present study is carried out to investigate the preliminary phytochemicals and bioactive compounds of leaf extracts by GC-MS. GC-MS analysis of leaf extracts revealed the presence of 50 compounds in all four solvents. Petroleum ether extract showed the presence of 18 compounds, whereas ethanol extract showed 12 compounds; chloroform and acetone extracts showed the presence of 10 compounds each. Phytol is the most significant compound in petroleum ether (28.72%), ethanol (32.99%), and acetone (41.61%) extracts. Whereas in chloroform extract, 1,2-benzenedicarboxylic acid (49.07%) is the most significant bioactive compound having antioxidant, antifouling and antimicrobial properties.