Bioaccessibility Of Polyphenols Research Articles

In Vitro Digestion of Vacuum-Impregnated Yam Bean Snacks: Pediococcus acidilactici Viability and Mango Seed Polyphenol Bioaccessibility.

This study investigates the in vitro digestion of vacuum-impregnated yam bean snacks enriched with Pediococcus acidilactici and mango seed polyphenols, focusing on bacterial survival and polyphenol bioaccessibility. The snacks were prepared by vacuum impregnation (VI) with solutions containing either mango seed extract, P. acidilactici, or a combination of both, followed by dehydration. The antimicrobial activity of the treatments was assessed against pathogens, revealing limited effectiveness, likely due to insufficient concentrations of mango seed extract and the intrinsic resistance of the bacteria. VI of mango seed extract improved the total soluble phenols (TSP) content up to 400% and maintained the initial probiotic concentration (106 cell/mL). In vitro digestion was performed to simulate gastrointestinal conditions, measuring the stability of TSP and the survival of P. acidilactici. The results indicated that the viability of P. acidilactici fluctuated throughout the digestion process (106 to 104 log UFC/g), the polyphenols showed varying degrees of bioaccessibility (11 to 30%), and the TSP content in the intestinal fraction ranged from 1.95 to 6.54 mg GAE/g. The study highlights the potential of VI for incorporating functional components into plant-based snacks, though further optimization is necessary to enhance the stability of P. acidilactici and the effectiveness of the bioactive ingredients.

Establishing a novel covalent complex of wheat gluten with tea polyphenols: Structure, digestion, and action mechanism

Plant-based proteins represent a more sustainable alternative, the approaches to modify and enhance their functionality and application are focused on. Covalent interaction could significantly modify the structure and function properties of protein. This study investigated the effects of covalent interaction between wheat gluten and tea polyphenols on the structure, aggregation, stability, and digestive properties of their covalent complex, as well as the possible action mechanism. The results showed that tea polyphenols could interact with gluten via covalent bonds (CN and/or CS), while tea polyphenols also acted as a bridge connecting gluten molecules, thus making covalent complex to show the larger particle sizes. This covalent interaction significantly changed the secondary structure, tertiary structure, and surface hydrophobicity of gluten. Moreover, covalent complex exhibited the high polyphenols bioaccessibility during in vitro digestion. The peptide bonds of covalent complex were mainly broken in gastric digestion, while the covalent bonds between tea polyphenols and gluten were completely destroyed in intestinal digestion. In addition, their digestates exhibited excellent antioxidant capability. All results suggest that wheat gluten have potential to prepare functional carrier for transporting active compounds and protecting them during digestion.

Repercussion of manufacture and digestion process of foods enriched with sesame flour on the antioxidant response of human hepatocyte cultures

As natural bioactive compounds with recognized antioxidant power, polyphenols may impact on several diseases associated with oxidative stress. In this study, defatted sesame flour (DSF) was investigated as a potential source of dietary polyphenols and a functional ingredient. Additionally, the impact of manufacture and digestion processes on the biological efficacy of the resulting extracts in the HepG2 cell line was explored. For this purpose, polyphenolic extracts were obtained from three sources: DSF, a control sweet cookie formulation and 10% DSF-enriched cookies. In addition, extracts of potentially bioaccessible polyphenols from in vitro digested cookies were collected. Cells were incubated with all these extracts and then injured with H2O2. Intracellular oxidative state and viability, antioxidant enzymes activities, glutathione (GSH) content, and oxidation of proteins and lipids were analyzed. The results showed DSF pro-oxidant actions on the cellular redox state, which hormetically enhanced the antioxidant response. Similarly, biological activity of DSF enriched cookies was found to persist after manufacture and digestion, promoting lipid peroxidation alleviation and GSH replenishment.By promoting an antioxidant response in cells, DSF may be considered for functional incorporation, offering preventive benefits and protective action to counterbalance oxidative damage. This study contributes to highlighting the benefits of DFS validating its final biological effects in a cellular model.

The role of processing on phenolic bioaccessibility and antioxidant capacity of apple derivatives

Fruit derivatives are commonly obtained by applying processing operations deemed responsible for the loss of phenol compounds, but very little information is available on the fate of phenols upon digestion of these products. The present study evaluated the effect of thermal and mechanical treatments, commonly applied to turn apple pulp into puree and homogenate, on phenolic bioaccessibility and antioxidant activity.Despite a 20 % decrease in polyphenols due to processing, their bioaccessibility was higher in apple derivatives (>20 %) compared to pulp (∼2 %). Polyphenol oxidase (PPO), inactivated by thermal treatments in apple derivatives but not in the pulp, was hypothesized to be responsible for this difference. Results acquired on an unprocessed PPO-free apple model, only featuring quercetin-3-glucoside and pectin, actually exhibited similar bioaccessibility as processed derivatives.The radical scavenging capacity was unaffected by the structural integrity of apples, indicating independence from the plant tissue's hierarchical arrangement. After digestion, radical scavenging capacity decreased in the real apple matrices, correlating with phenolic content, while it was retained in the apple model, further suggesting the pivotal food matrix role in modulating polyphenols bioaccessibility and subsequent biological activity.Translating these results to an industrial scale, processing conditions can be optimized not only to guarantee that the quality requirements are met, but also to achieve desired nutritional benefits.

Interactions of White Mugwort (Artemisia lactiflora Wall.) Extract with Food Ingredients during In Vitro Gastrointestinal Digestion and Their Impact on Bioaccessibility of Polyphenols in Various Model Systems.

The bioaccessibility of phytochemicals is an important factor for new functional food design. The interaction of white mugwort extract (FE) and food ingredients (coconut oil, egg white albumen, brown rice powder, inulin, and mixtures thereof) was determined after in vitro digestion to inform the development of a functional soup for an aging population. Coconut oil exerted a protective effect on polyphenols, showing the highest bioaccessibility (62.9%) and antioxidant activity after intestinal digestion (DPPH 12.38 mg GAE/g DW, FRAP 0.88 mol Fe(ll)/g DW). In contrast, egg white albumen had the most significant negative effect on the polyphenol stability, resulting in the lowest bioaccessibility (12.49%). Moreover, FE promoted the emulsion stability and delayed starch digestion by inhibiting amylase activity via non-specific polyphenol-protein interactions, resulting in a decrease in the total reducing sugars (TRS) released during digestion. It also limited the protein digestion, probably due to the complex formation of polyphenols and proteins, consequently reducing the bioaccessibility of both amino acids and polyphenols. These findings provide useful information for designing functional food products that could promote the bioaccessibility and bioactivity of natural extracts.

Alginate edible films as delivery systems for green tea polyphenols

This study investigated the potential of alginate edible films to act as vehicles for the delivery of polyphenols to the intestinal tract. Sodium alginate (0.5–2% w/v) and green tea extract (GTE, 25–50% w/w) were combined to form films with different microstructural properties via the casting method, where their physical, mechanical and barrier properties were analysed. Release studies into a 50% ethanol (v/v) food simulant and under simulated in vitro digestion were also conducted, and the resulting kinetics of polyphenols release was investigated. Composite alginate films with 25% w/w GTE (E∼2500 MPa; EAB∼14%; k∼0.04) showed significantly enhanced mechanical properties and slower rates of polyphenol release than samples with 50% w/w GTE (E∼6000 MPa; EAB∼6%; k∼0.18). Polyphenols entrapped within the 2% alginate – GTE films were successfully released during digestion (∼54%), demonstrating their bioaccessibility and availability for absorption by the gastrointestinal tract. The bioaccessibility of green tea polyphenols was significantly enhanced by films with good sustained-release effect (2% alginate – GTE, C/C0 = 54.41 ± 0.75%) respect to the free GTE (C/C0 = 33.73 ± 6.57%). These findings highlight the versatility of bioactive alginate edible films and create a blueprint for the design of sustainable active packaging alternatives with dual functionality.

Complexation With Plant-Based Proteins Enhances Bioaccessibility of Select Blueberry Polyphenols in Comparison to Whey Protein

Complexation With Plant-Based Proteins Enhances Bioaccessibility of Select Blueberry Polyphenols in Comparison to Whey Protein

Effect and mechanisms of thermal sterilization methods on the in vitro phenolic bioaccessibility of rose tea with milk

Rose polyphenols, key functional components in roses, require adequate bioaccessibility for their health benefits, subject to influence by food components and processing. Investigating the impact of various thermal sterilization methods on the bioaccessibility of rose polyphenols in rose tea with milk and the underlying mechanisms, our findings indicated a significant increase in bioaccessibility following treatment at 85 °C/30 min. Conversely, 121 °C/15 min treatment decreased bioaccessibility. Examining the interaction between β-casein in milk and rose polyphenols under different sterilization conditions, SEM and particle size analysis revealed binding, with fluorescence spectroscopy indicating non-covalent bonds. Binding forces followed the order 121 °C > 85 °C > 25 °C. Notably, at 85 °C, non-covalent binding improved polyphenol bioaccessibility, while the intensified binding at 121 °C decreased it. SDS-PAGE and amino acid analysis confirmed no covalent bond. This study establishes a theoretical basis for selecting thermal sterilization temperatures for milk-flower combinations, considering polyphenol bioaccessibility.

Comparative Analysis of Black Chokeberry (Aronia melanocarpa L.) Fruit, Leaves, and Pomace for Their Phytochemical Composition, Antioxidant Potential, and Polyphenol Bioaccessibility.

The study aims to compare the nutrient composition, antioxidant potential, and polyphenol bioaccessibility of the fruit, leaves, and pomace of black chokeberry. Phytochemical characterization, antioxidant activity, and the effect of in vitro gastrointestinal digestion on the individual phenolic compounds of fruit, leaves, and pomace of black chokeberry were assessed. Results showed that leaves had a higher content of polyphenols (61.06 mg GAE/g dw), flavonoids (8.47 mg QE/g), and tocopherols (1172.20 mg/kg) than fruit (27.99 mg GAE/g dw polyphenols, 5.23 mg QE/g flavonoids, 38.48 mg/kg tocopherols) and pomace (22.94 mg GAE/g dw polyphenols, 1.89 mg QE/g flavonoids and 157.19 mg/kg tocopherols), with superior in vitro antioxidant activity. Chlorogenic acids were the dominant phenolic compounds in black chokeberry undigested samples (2.713 mg/g in fruit, 17.954 mg/g in leaves, and 1.415 mg/g in pomace) but are poorly absorbed (bioaccessibility index in intestinal phase of 28.84% for fruit, 8.81% for leaves, and 31.90% for pomace). Hydroxybenzoic acids were highly stable in leaves and fruit during simulated digestion and had high bioaccessibility. In conclusion, residues from black chokeberry processing are also valuable sources of bioactive compounds, but the pomace had higher polyphenol bioaccessibility than leaves and might be a promising supplement for the food industry.

Evaluating the bioaccessibility and antioxidant activity of polyphenols extracted from vegetables by‐product

Evaluating the bioaccessibility and antioxidant activity of polyphenols extracted from vegetables by‐product

Development of interpenetrating network hydrogels: Enhancing the release and bioaccessibility of green tea polyphenols

Green Tea polyphenols (GTP) are important bioactive compounds with excellent physiological regulation functions. However, they are easily destroyed by the gastric environment during digestion. In this work, a sodium alginate (SA)-gellan gum (GG) interpenetrating network (IPN) hydrogel was synthesized to protect and delivery GTP. The ratio of SA/GG significantly affects the network structure of IPN hydrogels and the performance of delivering GTP. The hydrogel formed by interpenetrating 20 % GG with 80 % SA as the main network had the highest water uptake (55 g/g), holding capacity (950 mg/g), and freeze-thaw stability, with springiness reaching 0.933 and hardness reaching 1300 g, which due to the filling effect and non-covalent interaction. Rheological tests showed that the crosslink density of IPN hydrogel in SA-dominated network was improved by the addition of GG to make it better bound to GTP, and the higher water uptake meant that the system could absorb more GTP-containing solution. This IPN hydrogel maintained 917.3 mg/g encapsulation efficiency at the highest loading capacity (1080 mg/g) in tests as delivery system. In in vitro digestion simulations, owing to the pH responsiveness, the IPN hydrogel reduced the loss of GTP in gastric fluid, achieving a bioaccessibility of 71.6 % in the intestinal tract.

Impact of Ohmic Heating and Ultrasound Pretreatments on Oil Absorption and Other Quality Parameters of Fried Potato

Impact of Ohmic Heating and Ultrasound Pretreatments on Oil Absorption and Other Quality Parameters of Fried Potato

Blackcurrant (Fruits, Pomace, and Leaves) Phenolic Characterization before and after In Vitro Digestion, Free Radical Scavenger Capacity, and Antioxidant Effects on Iron-Mediated Lipid Peroxidation.

Blackcurrant (Ribes nigrum L.) is a berry bush widely cultivated in Europe for producing juices, jams, jellies, and syrups. In addition to berries, blackcurrant leaves and pomace, as byproducts, have also been shown to have health-promoting effects. Static digestion, simulating oral, gastric, and small intestinal digestion, was applied, and blackcurrant leaves, fruits, and pomace and the polyphenol bioaccessibility were evaluated in terms of recovery index. The results were related to sample type, and the recovery index presented higher values in the case of fruits, indicating this morphological part of blackcurrant as the most bioaccessible. The antioxidant potential of blackcurrant was evaluated using four different methods, with the leaves proving to be a significant and powerful antioxidant compared to fruits and pomace. The counteracting potential of inhibiting the oxidation process was evaluated using in vitro-induced lipid peroxidation and the inhibition potential of superoxide and hydroxyl anions. The antioxidant evaluation and the inhibition of biological and non-biological radicals indicate the leaf extract is the most powerful antioxidant studied. Also, the results proved that not only fruits but also the blackcurrant byproducts (pomace and leaves) are promising sources of bioaccessible antioxidants with potential benefits in animal nutrition.

Total phenolic content of microwaved, pan-heated and stewed, herbs and spices

Culinary herbs and spices help improve food flavour in cuisine all over the globe and possess some of the richest sources of polyphenols in edible plant foods which are linked to human health benefits. Food processing can affect these constituents and therefore may impact the health benefits these plants may confer. This study aimed to assess the impact of 3 commonly used domestic cooking methods on the total phenolic content, assessed via the total phenolic content (TPC) assay, on 15 culinary herbs and 12 spices, fresh and dried (50 samples total). Herbs and spices uncooked (aqueous extracts), microwaved with water (4 min), pan-heated without medium in a non-stick pan (5, 10, 15 and 20 min) and stewed with water (30, 60 and 120 min) were tested (n = 3) for TPC, expressed in gallic acid equivalent (GAE). TPC for uncooked plants were ranked from very low (below 50 GAE mg/100g) to very high (over 4000 GAE mg/100g). Dried herbs and spices were significantly higher in TPC than their fresh counterparts (P < 0.01). Despite exceptions to the general trend, overall microwaving increased the TPC by 18% compared to uncooked extracts (P < 0.01). Pan-heating in a non-stick pan caused a significant decrease in TPC, averaging 14% (p < 0.01), similar between the 4 lengths of times. Stewing significantly increased the TPC (p < 0.01) by 26% at 30 min, 39% at 60 min and 46% at 120 min. Therefore, polyphenol bioaccessibility in culinary herbs and spices decreased with dry pan-heating but increased with microwaving and stewing with water.

Optimization of fermentation process and characterization of non-alcoholic functional beverage from pigmented rice varieties

Traditional foods have recently gained importance because of their role in the United Nations’ sustainable development goals (SDG), ensuring health benefits and economic development. Fermented rice water (FRW) is a popular traditional drink among low socio-economic groups, especially in Southeast Asia. This study focused on optimizing fermentation conditions for sustainable food production and consumption of FRW using pigmented rice, followed by analyzing the nutritional, physicochemical and phytochemical properties. Fermenting cooked rice in water for 16 h at 37 °C produced bubbles, mild sourness and zero alcohol content. Flavors and sugar were added to optimized FRW as food additives to enhance palatability. Nutritional profiling showed that black flavored fermented rice water (FFRW) had the highest protein (0.06%), carbohydrate (13.25 g/100 mL) and calorific value (78.09 kcal/100 mL). However, brown FFRW had the highest vitamin B3 (3.12 mg/L) and mineral content. Phenolic and antioxidant activity was highest in black FFRW, followed by red and brown, with maximum bio-accessibility of polyphenols found in black FFRW (75.25%). All FFRW were microbiologically safe and stable during a storage period of 30 days at 4 °C. All FRW had >8 log CFU count, with black FFRW having the highest LAB count, indicating their possible probiotic potential in addressing malnutrition and undernourishment. Thus, FFRW holds the potential to be commercialized in the food and beverage sector due to its rich nutritional and functional properties. Furthermore, the consumption of FFRW will preserve traditional knowledge, ensure sustainable livelihoods and contribute to achieving development goals.

Exploring the bioaccessibility of polyphenols and glucosinolates from Brassicaceae microgreens by combining metabolomics profiling and computational chemometrics

Microgreens constitute natural-based foods with health-promoting properties mediated by the accumulation of glucosinolates (GLs) and phenolic compounds (PCs), although their bioaccessibility may limit their nutritional potential. This work subjected eight Brassicaceae microgreens to in vitro gastrointestinal digestion and large intestine fermentation before the metabolomics profiling of PCs and GLs. The application of multivariate statistics effectively discriminated among species and their interaction with in vitro digestion phases. The flavonoids associated with arugula and the aliphatic GLs related to red cabbage and cauliflower were identified as discriminant markers among microgreen species. The multi-omics integration along in vitro digestion and fermentation predicted bioaccessible markers, featuring potential candidates that may eventually be responsible for these functional foods' nutritional properties. This combined analytical and computational framework provided a promising platform to predict the nutritional metabolome-wide outcome of functional food consumption, as in the case of microgreens.

Time maters: Exploring the dynamics of bioactive compounds content, bioaccessibility and antioxidant activity during Lupinus angustifolius germination

Germination is a process that enhances the content of health-promoting secondary metabolites. However, the bioaccessibility of these compounds depends on their stability and solubility throughout the gastrointestinal tract. The study aimed to explore how germination time influences the content and bioaccessibility of γ-aminobutyric acid and polyphenols and antioxidant capacity of lupin (Lupinus angustifolius L.) sprouts during simulated gastrointestinal digestion. Gamma-aminobutyric acid showed a decrease following gastrointestinal digestion (GID) whereas phenolic acids and flavonoids exhibited bioaccessibilities of up to 82.56 and 114.20%, respectively. Although the digestion process affected the profile of phenolic acids and flavonoids, certain isoflavonoids identified in 7-day sprouts (G7) showed resistance to GID. Germination not only favored antioxidant activity but also resulted in germinated samples exhibiting greater antioxidant properties than ungerminated counter parts after GID. Intestinal digests from G7 did not show cytotoxicity in RAW 264.7 macrophages, and notably, they showed an outstanding ability to inhibit the production of reactive oxygen species. This suggests potential benefit in mitigating oxidative stress. These findings contribute to understand the dynamic interplay between bioprocessing and digestion in modulating the bioaccessibility of bioactive compounds in lupin, thereby impacting health.

In Vitro Gastrointestinal Digestion of Various Sweet Potato Leaves: Polyphenol Profiles, Bioaccessibility and Bioavailability Elucidation.

The chemical composition discrepancies of five sweet potato leaves (SPLs) and their phenolic profile variations during in vitro digestion were investigated. The results indicated that Ecaishu No. 10 (EC10) provided better retention capacity for phenolic compounds after drying. Furthermore, polyphenols were progressively released from the matrix as the digestion process proceeded. The highest bioaccessibility of polyphenols was found in EC10 intestinal chyme at 48.47%. For its phenolic profile, 3-, 4-, and 5-monosubstituted caffeoyl quinic acids were 9.75%, 57.39%, and 79.37%, respectively, while 3,4-, 3,5-, and 4,5-disubstituted caffeoyl quinic acids were 6.55, 0.27 and 13.18%, respectively. In contrast, the 3,4-, 3,5-, 4,5-disubstituted caffeoylquinic acid in the intestinal fluid after dialysis bag treatment was 62.12%, 79.12%, and 62.98%, respectively, which resulted in relatively enhanced bioactivities (DPPH, 10.51 μmol Trolox/g; FRAP, 8.89 μmol Trolox/g; ORAC, 7.32 μmol Trolox/g; IC50 for α-amylase, 19.36 mg/g; IC50 for α-glucosidase, 25.21 mg/g). In summary, desirable phenolic acid release characteristics and bioactivity of EC10 were observed in this study, indicating that it has potential as a functional food ingredient, which is conducive to the exploitation of the sweet potato processing industry from a long-term perspective.

Metabolomic insights into the profile, bioaccessibility, and transepithelial transport of polyphenols from germinated quinoa during in vitro gastrointestinal digestion/Caco-2 cell transport, and their prebiotic effects during colonic fermentation

The health-promoting activities of polyphenols and their metabolites originating from germinated quinoa (GQ) are closely related to their digestive behavior, absorption, and colonic fermentation; however, limited knowledge regarding these properties hinder further development. The aim of this study was to provide metabolomic insights into the profile, bioaccessibility, and transepithelial transport of polyphenols from germinated quinoa during in vitro gastrointestinal digestion and Caco-2 cell transport, whilst also investigating the changes in the major polyphenol metabolites and the effects of prebiotics during colonic fermentation. It was found that germination treatment increased the polyphenol content of quinoa by 21.91%. Compared with RQ group, 23 phenolic differential metabolites were upregulated and 47 phenolic differential metabolites were downregulated in GQ group. Compared with RQ group after simulated digestion, 7 kinds of phenolic differential metabolites were upregulated and 17 kinds of phenolic differential metabolites were downregulated in GQ group. Compared with RQ group after cell transport, 7 kinds of phenolic differential metabolites were upregulated and 9 kinds of phenolic differential metabolites were downregulated in GQ group. In addition, GQ improved the bioaccessibilities and transport rates of various polyphenol metabolites. During colonic fermentation, GQ group can also increase the content of SCFAs, reduce pH value, and adjust gut microbial populations by increasing the abundance of Actinobacteria, Bacteroidetes, Verrucomicrobiota, and Spirochaeota at the phylum level, as well as Bifidobacterium, Megamonas, Bifidobacterium, Brevundimonas, and Bacteroides at the genus level. Furthermore, the GQ have significantly inhibited the activity of α-amylase and α-glucosidase. Based on these results, it was possible to elucidate the underlying mechanisms of polyphenol metabolism in GQ and highlight its beneficial effects on the gut microbiota.

Colloid‐encapsulated sugarcane polyphenols and their impact on in vitro starch digestibility: enhancing bioaccessibility and α‐amylase inhibitory effect

SummaryThis study focuses on encapsulation of sugarcane polyphenols (SPs) to assess its impact on their bioaccessibility and efficacy in inhibiting starch digestion, thereby contributing to glycaemic control strategies. The hypothesis posits that encapsulation enhances both the bioaccessibility and functional effectiveness of SPs. Carboxymethyl cellulose (CMC) and carrageenan (CAR) were employed as encapsulating agents. The methodology included an evaluation of α‐amylase inhibitory activity and the bioaccessibility of SPs across various encapsulation formulations in vitro digestion, highlighting a specific formulation that combines 0.5% CMC and 0.5% CAR to encapsulate 0.4% SPs. Findings reveal that this formulation significantly increases SPs' bioaccessibility and exhibits the highest α‐amylase inhibitory activity, suggesting its potential to support a low glycaemic index (GI) diet (GI = 54.7). It was observed that the GI is significantly inversely correlated with the bioaccessibility of total polyphenols and proanthocyanidins in SPs, while the bioaccessibility of total flavonoids in SPs showed no significant effect on pGI. Interestingly, SPs encapsulated with CMC were less effective in inhibiting starch digestibility than unencapsulated SPs, attributed to the tight binding of CMC with polyphenols, which limits their interaction with starch and α‐amylase. The study highlights the crucial influence of encapsulation materials on polyphenol functionality and emphasises the need for strategic encapsulation in modulating glycaemic responses. These findings advance the understanding of how processing technologies can optimise the health benefits of dietary bioactives, guiding future research towards functional applications and dietary interventions for glycaemic control.