Bioaccessibility Measurements Research Articles

A comparison of the bioaccessible calcium supplies of various plant-based products relative to bovine milk

Calcium deficiency is widespread globally, especially in diets with minimal consumption of dairy. It is therefore important to identify plant-based sources of calcium that can make a meaningful contribution to calcium intakes for populations following diets with a minimum supply of dairy products. The best sources of calcium have a high calcium content and bioavailability. Therefore, we evaluated the gross and bioaccessible calcium supplies of 25 plant-based products from 5 food groups considered to be good and important sources of calcium. Bioaccessible calcium was examined using the INFOGEST static digestion model in which isotopically labelled 43Ca was used as a tracer of reagent calcium to improve accuracy of bioaccessibility measurements. The gross calcium content varied widely amongst all the food products, ranging between 7.48 and 959 mg/100 g fresh weight (fw), with approximately 50 % of the products being equivalent to or surpassing the calcium content of skimmed milk. Bioaccessibility of calcium was equally variable, ranging from about 0.1 – 50 %. The lowest bioaccessibility (<10 %) was found in spinach, plant-based beverages, tofu, dried figs and tahini and was attributed to: 1. the high content of oxalate and phytate in some of the products, and 2. the low solubility of tricalcium phosphate which was used for fortification in the plant-based beverages. The remaining products generally had a high bioaccessibility that was similar to, or higher than that of skimmed milk (∼30 %). When both bioaccessibility and recommended serving portions were considered, only 3 products were identified as good sources of calcium, requiring 0.2 – 1.4 servings to equal the bioaccessible supply from skimmed milk. The top three sources of plant-based calcium identified were kale, finger millet and fortified white bread in that order, with kale providing 5 times more bioaccessible calcium than 1 serving of skimmed milk. Moderate sources of calcium where 1.5 – 3 servings was equivalent to 1 serving of skimmed milk included wholemeal bread, some bean varieties (black chickpeas, chickpeas, kidney beans, peas), broccoli, cabbage and almond drink. The rest of the products were either of low calcium content, poor bioaccessibility, and/or not consumed in sufficient quantities to make a significant contribution to daily requirements. White bread was a good source of calcium as it was fortified with calcium carbonate and this suggests that mandatory widescale fortification of staple cereals with this form of calcium should be considered a viable approach to augment dietary calcium intakes in vulnerable populations. Low bioaccessibility of fortified calcium in plant-based beverages, often marketed as good sources of calcium, suggests the need for regulation and for further in vivo studies to validate bioavailability of calcium in these products.

Digitalizing the TIM-1 Model using Computational Approaches-Part One: TIM-1 Data Explorer.

The TIM-1 gastrointestinal model is one of the most advanced in vitro systems currently available for biorelevant dissolution testing. This technology, the initial version of which was developed nearly 30 years ago and has been subject to a number of significant updates over this period, simulates the dynamic environment of the human gastrointestinal tract, including pH, transfer times, secretion of bile, enzymes, and electrolytes. In the pharmaceutical industry, the TIM-1 system is used to support drug product design and provide a biopredictive assessment of drug product performance. Typically, the bioaccessibility data sets generated by TIM-1 experiments are used to qualitatively compare formulation performance, and the use of bioaccessibility data as inputs for physiologically based pharmacokinetic (PBPK) modeling for quantitative predictions is limited. To expand the utility of the TIM-1 model beyond standard bioaccessibility measurements (which define the fraction available for absorption), we have developed a computational tool, TIM-1 Data Explorer, to describe the fluid and mass balance within the TIM-1 system. The use of this tool allows a detailed inspection and in-depth interpretation of the experimental data. In addition to mass balance calculation, this model also can be used to describe the critical processes a drug substance would undergo during a TIM-1 experiment, such as dissolution, precipitation on transfer from the stomach to duodenum, and redissolution. The TIM-1 Data Explorer was validated in two case studies. In the first case study with paracetamol, we have shown the ability of the simulator to adequately describe mass transfer events within the TIM-1 system, and in the second study with a weakly basic in-house compound, PF-07059013, the TIM-1 Data Explorer was successfully used to describe dissolution and precipitation processes.

Inhalation bioaccessibility of inhaled triazole fungicides and health risk assessment during spraying.

Ambient air pollution caused by pesticide drift has received great attention. To accurately evaluate the health risk of inhaled pesticides, bioaccessibility should be considered. However, methods to reliably assess pesticide residues remain limited, hindering the precise estimation of exposure assessment. We aimed to optimize an in vitro method for the inhalation bioaccessibility (IBA) measurement of triazole fungicides and to incorporate this into inhalation exposure assessment during pesticide spraying. The IBA of triazole fungicides increased logarithmically with extraction duration, plateauing after 6h. The frequency of agitation displayed a similar pattern, whereas the ratio of solid to liquid between 1/1500 and 1/250 was considerably negatively associated. The predicted values (35.9-53.5%) for IBA based on optimized methodological parameters determined using a response surface methodology showed an acceptable deviation from experimental values (30.7-50.8%), suggesting feasibility for in vitro IBA measurement. Incorporating IBA into calculations of inhalation exposure amount (IE) yielded a value of 8.5 × 10-7 -2.1 × 10-5 mg kg-1 day-1 , a 50-68% reduction compared to IE based on total amount. Additionally, the safety exposure threshold was determined for triazole fungicides using benchmark dose modelling of data from lung A549 cell proliferation toxicity assays, and in this context, margin of exposure (MOE) values were calculated to be within an acceptable level. This in vitro method supplements bioaccessibility evaluation based on pesticide inhalation exposure, along with the risk to human health. © 2023 Society of Chemical Industry.

Research progress on bioaccessibility of persistent organic pollutants in oral exposure matrices

<p indent="0mm">Persistent organic pollutants (POPs) have been widely concerned due to their bioaccumulation, environmental persistence, and strong toxic effects on humans and organisms. Oral digestion via food, dust, and soil is the most important route for humans to uptake POPs. However, not all POPs that enter human body are bioavailable, thus exposure risk assessment based on the total amount of POPs in matrices would overestimate the resulting health risks. Measurement of bioaccessibility using <italic>in vitro</italic> gastrointestinal (GI) digestive simulation has been used to predict the bioavailability of POPs. In this paper, the impacts of <italic>in vitro</italic> experimental approaches, properties of oral matrices, compositions of digestive fluid, and properties of POPs on the bioaccessibility of POPs in oral exposure matrices are comprehensively reviewed and analyzed. The dynamic <italic>in vitro</italic> GI digestive simulation improved the accuracy of the results compared with traditional static GI digestive simulation. The digestion of nutrients in the GI tract and cooking methods affect the distribution of POPs between matrices and digestive fluids, thus affecting bioaccessibility. Lipids generally enhanced the bioaccessibility of most POPs because they promote the formation of stable fatty acid-bile salt micelles and increase the solubility of POPs in digestive fluid. However, this solubilization effect of mixed micelles does not apply to POPs with very high hydrophobicity because they prefer to distribute in the undigested food matrix. The bioaccessibility of POPs is mostly positively correlated with the carbohydrate content of food, whereas the effects of protein and dietary fiber were uncertain. Particle size and organic matter content of dust or soil are important factors affecting the bioaccessibility of POPs. The smaller the particle size of the dust or soil, the larger specific surface area, and the digestive fluid displayed strong capacity to dissolve POPs, thus increasing their bioaccessibility. Organic matter has a significant effect on the adsorption and desorption of POPs from dust or soil. The higher the organic matter content, the more POPs will be adsorbed, thus hindering the release of POPs from the matrices. Bile salts and various digestive enzymes such as pepsin and trypsin can also change the bioaccessibility of POPs by affecting the digestion process of matrices. There is generally a negative relationship between the bioaccessibility of POPs and their log<italic>K</italic>_OW values. The higher the hydrophobicity of POPs, the more difficult it is to dissolve in the digestive fluid. Different migration routes of POPs will also lead to different bioaccessibility, and the bioaccessibility of POPs adsorbed into the matrices is higher than that of POPs existing as a component of matrices. When considering bioaccessibility, the estimated daily intake of POPs from a matrix will decrease and the estimated exposure risk will therefore be reduced. Thus the contribution of POPs via different matrices to total human exposure should be re-examined. Collectively, bioaccessibility should be fully considered in the assessment of oral exposure and health risk to ensure accurate results. There are still some limitations in the current research. Firstly, results obtained using different <italic>in vitro</italic> methods varied widely and validation with <italic>in vivo</italic> data is lacking. Secondly, current studies mostly focused on only one oral matrix which cannot represent rich daily diets and different exposure scenarios among different populations. Thirdly, there is still a big gap in the research on the bioaccessibility of emerging POPs and the mixed exposure to multiple POPs. This paper provides an important theoretical basis for the accurate assessment of the human exposure risk of POPs, and for formulating measures to reduce the oral exposure risk of POPs in different populations.

Mercury bioaccessibility in fish and seafood: Effect of method, cooking and trophic level on consumption risk assessment

The bioaccessible Hg fraction in fish and seafood commonly present in the Mediterranean diet was assessed through three distinct in vitro extraction schemes. Each extraction method provided different results, highlighting the lack of a universal methodology to estimate mercury (Hg) bioaccessibility in those matrices. Bioaccessible Hg fractions ranged from 10 to nearly 90% of total mercury (T-Hg) and increased in predator species (Swordfish - Xiphias gladius, Blue Shark - Prionace glauca and Tuna - Thunnus sp.). Among the three extractions tested, the Unified Bioaccessibility Method (UBM) provided the highest estimation of Hg bioaccessibility for consumers. The tested cooking procedures (frying, grilling and steaming) considerably reduced the bioaccessible fraction. Results indicate that bioaccessible Hg found in ingested fish and seafood is far below the levels set by the current safety risk assessment legislation. These findings highlight the importance of integrating bioaccessibility measurements in food safety legislation.

Magnetic poly(β-cyclodextrin) combined with solubilizing agents for the rapid bioaccessibility measurement of polycyclic aromatic hydrocarbons in soils

The rapid determination of the bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soils is challenging due to their slow desorption rates and the insufficient extraction efficiency of the available methods. Herein, magnetic poly(β-cyclodextrin) microparticles (Fe3O4@PCD) were combined with hydroxypropyl-β-cyclodextrin (HPCD) or methanol (MeOH) as solubilizing agents to develop a rapid and effective method for the bioaccessibility measurement of PAHs. Fe3O4@PCD was first validated for the rapid and quantitative adsorption of PAHs from MeOH and HPCD solutions. The solubilizing agents were then coupled with Fe3O4@PCD to extract PAHs from soil-water slurries, affording higher extractable fractions than the corresponding solution extraction and comparable to or higher than single Fe3O4@PCD or Tenax extraction. The desorption rates of labile PAHs could be markedly accelerated in this process, which were 1.3–12.0 times faster than those of single Fe3O4@PCD extraction. Moreover, a low HPCD concentration was sufficient to achieve a strong acceleration of the desorption rate without excessive extraction of the slow desorption fraction. Finally, a comparison with a bioaccumulation assay revealed that the combination of Fe3O4@PCD with HPCD could accurately predict the PAH concentration accumulated in earthworms in three field soil samples, indicating that the method is a time-saving and efficient procedure to measure the bioaccessibility of PAHs.

Evaluation of the bioaccessibility of a carotenoid beadlet blend using an in vitro system mimicking the upper gastrointestinal tract.

The release characteristics of a unique blend of carotenoid beadlets designed to increase bioavailability were tested using the dynamic gastrointestinal model TIM‐1. Individual carotenoid bioaccessibility peaks were observed over approximately 3–4 hr in the order of lutein and zeaxanthin first, followed by lycopene, and then finally α‐ and β‐carotene; when tested as a beadlet blend or when the beadlets were compressed into tablets. Bioaccessibility measurements of 7%–20% were similar to those previously reported in literature and comparable between the two formulations, beadlet blend and tablet formulations. Total recovery of carotenoids from all compartments ranged from 70% to 90% for all carotenoids, except lycopene where almost 50% was unrecoverable after digestion in the TIM system.

Predicting the relative oral bioavailability of naturally occurring As, Cd and Pb from in vitro bioaccessibility measurement: implications for human soil ingestion exposure assessment

Chestnut soils developed over mineralized areas of southwestern Spain are characterized by high baseline concentrations of geogenic trace elements, notably Pb (up to 14,562mgkg-1), As (up to 346mgkg-1) and Cd (up to 319mgkg-1), which could pose an unacceptable risk to the health of the hand-harvest workers who are being exposed to surface soil by incidental ingestion and dermal contact. Oral bioaccessibility, as determined by simulating the human digestion process in a test-tube environment (Unified BARGE Method), followed the increasing order of As (3.1%) < Pb (21.5%) < Cd (35.6%) in the gastric phase, and As (3.4%) < Pb (4.5%) < Cd (13.2%) in the gastrointestinal extract. Relative bioavailability (RBA) of As (3.1-2.1%), Pb (17.8-17.5%) and Cd (34.4-23.3%), predicted from in vitro bioaccessibility measurement through linear regression models, seems to be influenced not only by the pH and composition of digestive solutions but also by geochemical partitioning of trace elements among the soil constituents. The integration of RBA data in the risk calculations had a considerable effect on the site-specific risk estimations. After RBA adjustment, the level of carcinogenic risk associated with As exposure (< 1.5E-06) and the hazard index for non-carcinogens (< 0.4) was within the regulatory limits, indicating that occupational risks are not of concern. Hence, it can be concluded that the use of a default value of 100% for bioavailability may dramatically overestimate the chronic exposure to geologically sourced trace elements.

Tracing the sources of bioaccessible metal(loid)s in urban environments: A multidisciplinary approach

Understanding the links between sources of magnetic particles and bioaccessibility of metal(loids) in environmental sampling media is crucial for better evaluating human health risks, although relevant information in the scientific literature is scarce. Here, soil, road and house dust samples from a heavy industrial area in Greece were characterized in a multidisciplinary study combining magnetic measurements, SEM/EDS analyses, bioaccessibility measurements and Pb isotopic analyses of bioaccessible Pb. The oral and inhalable bioaccessible fractions of As, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn were assessed by applying simulated gastric and lung solutions. SEM/EDS analysis revealed the abundant presence of anthropogenic Fe-containing spherules of industrial origin in all sampling media, often containing minor contents of Cr, Cu, Mn, Pb and Zn. The inhalation bioaccessibility (%) in all environmental compartments was higher than the oral one for most elements analyzed in the present study. Clear associations between magnetic susceptibility and bioaccessible amounts of most of analyzed elements were encountered for the soil and road dust. The isotopic analyses of bioaccessible Pb showed that there are significant differences in the isotopic ratios between total and bioaccessible Pb. We conclude that Pb solubilized by the simulated gastric and lung extractions is principally anthropogenic, representing a mixture of industrial Pb and Pb related to the past usage of leaded petrol. Low values of 206Pb/207Pb were accompanied by high bioaccessible contents of Cd, Pb and Zn indicating that anthropogenic (mostly industrial) sources exert influence on the bioaccessible forms of these metals. Coupling magnetic and bioaccessibility measurements with stable isotopic technique of bioaccessible Pb is more reliable for determining Pb and other metal sources with high oral and inhalation bioaccessibility.

A stable isotope approach to accurately determine iron and zinc bioaccessibility in cereals and legumes based on a modified INFOGEST static in vitro digestion method

The establishment of the INFOGEST in vitro static digestion method, a standardized international consensus, was an important milestone in the field of food digestion. We evaluated the contribution of iron and zinc in reagents used in the INFOGEST method in relation to sample iron and zinc and the potential interference of reagent-derived iron and zinc with bioaccessibility measurements. In most cases, reagent-derived iron and zinc contributed more than 50% of the total iron or zinc in the digesta containing selected cereals and legumes. Moreover, the chemical behaviour of reagent-derived iron and zinc was matrix dependent such that the application of a blanket blank correction was not appropriate. We therefore propose an improved approach involving isotopic labelling of reagent iron and zinc in order to discriminate between reagent-derived and sample-derived iron and zinc in each matrix. This stable isotope approach could improve the accuracy and reliability of iron and zinc bioaccessibility studies.

Gastric bioaccessibility and human health risks associated with soil metal exposure via ingestion at an E-waste recycling site in Kumasi, Ghana

Over 1000 people make a living by processing electronic and electrical waste (E-waste) and scrap metals for the recovery of valuable metals and integrated circuits at Dagomba Line, Kumasi, Ghana. The processing includes activities such as dismantling, open burning and open dumping of E-waste which can potentially release toxic metals into the environment and thus impact the health of recyclers and nearby residents. This study investigated the distribution of toxic metals (As, Cd, Cr, Cu, Hg, Ni, Pb, Sb and Zn) in surface soils at the E-waste recycling sites and determined the associated human health risk via ingestion incorporating bioaccessibility measurements. Metal concentrations in the activity sites were highly elevated, significantly higher than those in the surrounding area and exceeded international soil quality guidelines such as the Canadian soil quality guidelines for residential landuse and the Dutch Intervention Value. Bioaccessibility was high for Pb (70.8%), Cd (64.1%), Cu (62.3%) and Ni (53.6%) which could be credited to the existence of oxidized species as a result of the E-waste burning. Non-carcinogenic effects were unacceptably high (hazard indices > 1) at 14 out of 31 sites, and the cancer risk for arsenic for adult workers was greater than 1 × 10-5 at five of the sampling sites.

Gut microbes modulate bioaccessibility of lead in soil

Metabolic uptake of lead (Pb) is controlled by its bioaccessibility. Most studies have examined bioaccessibility of Pb in the absence of gut microbes, which play an important role in the metabolic uptake of nutrients and metal(loid)s in intestine. In this study, we examined the effect of three gut microbes, from various locations in the gut, on the bioaccessibility of soil ingested Pb. The gut microbes include Lactobacillus acidophilus, Lactobacillus rhamnosus and Escherichia coli. Lead toxicity to these three microbes was also examined at various pH values. Bioaccessibility of Pb was measured using gastric and intestinal extractions. Both Pb spiked and Pb-contaminated shooting range field soils were used to measure Pb bioaccessibility in the presence and absence of gut microbes. The results indicated that Pb toxicity to gut microbes, as measured by LD50 value, decreased with increasing pH, and was higher for Lactobacillus species. Gut microbes decreased the bioaccessible Pb; the effect was more pronounced at low pH, mimicking gastric conditions than in conditions closer to the intestine. Lead adsorption by these microbes increased at the higher pH tested, and E. coli adsorbed higher amounts of Pb than did the Lactobacillus species. The effect of gut microbes on reducing Pb bioaccessibility may be attributed to microbially-induced immobilization of Pb through adsorption, precipitation, and complexation reactions. The study demonstrates that bioaccessibility and subsequently bioavailability of metal(loid)s can be modulated by gut microbes, and it is important to undertake bioaccessibility measurements in the presence of gut microbes.

Temporal Evolution of PAHs Bioaccessibility in an Aged-Contaminated Soil during the Growth of Two Fabaceae.

Polycyclic aromatic hydrocarbons (PAHs) are health-concerning organic compounds that accumulate in the environment. Bioremediation and phytoremediation are studied to develop eco-friendly remediation techniques. In this study, the effects of two plants (Medicago sativa L. and Trifolium pratense L.) on the PAHs’ bioaccessibility in an aged-contaminated soil throughout a long-term rhizoremediation trial was investigated. A bioaccessibility measurement protocol, using Tenax® beads, was adapted to the studied soil. The aged-contaminated soil was cultured with each plant type and compared to unplanted soil. The bioaccessible and residual PAH contents were quantified after 3, 6 and 12 months. The PAHs’ desorption kinetics were established for 15 PAHs and described by a site distribution model. A common Tenax® extraction time (24 h) was established as a comparison basis for PAHs bioaccessibility. The rhizoremediation results show that M. sativa developed better than T. pratense on the contaminated soil. When plants were absent (control) or small (T. pratense), the global PAHs’ residual contents dissipated from the rhizosphere to 8% and 10% of the total initial content, respectively. However, in the presence of M. sativa, dissipation after 12 months was only 50% of the total initial content. Finally, the PAHs’ bioaccessible content increased more significantly in the absence of plants. This one-year trial brought no evidence that the presence of M. sativa or T. pratense on this tested aged-contaminated soil was beneficial in the PAH remediation process, compared to unplanted soil.

Investigating the Effect of Medicago sativa L. and Trifolium pratense L. Root Exudates on PAHs Bioremediation in an Aged-Contaminated Soil

Polycyclic aromatic hydrocarbons (PAH) are persistent organic compounds of major concern that accumulate in the environment, especially soils, and require remediation. Researches to develop bioremediation and phytoremediation (alternative eco-friendly technologies) are being conducted. First, a bioaccessibility measurement protocol was adapted to a brownfield soil using Tenax® beads in order to compare PAHs bioaccessibility in soil samples. PAHs desorption kinetics were established, described by a site distribution model, and a common extraction time was calculated (48 h). Second, the role of two Fabaceae (Medicago sativa L. or Trifolium pratense L.) root exudates in enhancing PAHs bioaccessibility and biodegradation in the studied soil was evaluated during microcosms experiments (28 °C). The CO2 emissions were significantly higher in presence of T. pratense exudates; the dehydrogenase activities showed improvements of the soil microbial activity in presence of two types of root exudates compared to untreated soil samples; the PAHs residual contents decreased more in untreated samples than in the presence of T. pratense exudates; and M. sativa exudates lowered PAHs bioaccessibility but not residual contents.

Polydopamine-coated polyethylene sieve plate as an efficient and convenient adsorption sink for the bioaccessibility prediction of PAHs in soils

Bioaccessibility measurements of polycyclic aromatic hydrocarbons (PAHs) in soils are significant for exposure risk assessment. The current physicochemical methods require tedious operation processes, underestimate the actual risks, or are unsuitable for high organic content soils. In this work, an efficient and convenient method based on polydopamine-coated polyethylene sieve plate (PDA@PESP) and hydroxypropyl-β-cyclodextrin (HPCD) was developed to predict the bioaccessibility of PAHs in multi-type soils. The PDA@PESP can be prepared via in situ self-polymerization, allowing to extract PAHs from HPCD solution quantitatively and rapidly. When applied to evaluate the bioaccessibility with PDA@PESP as an adsorption sink and HPCD as a diffusive carrier, the proposed method can significantly improve the extractable fraction of PAHs compared to single HPCD extraction in particular for high organic carbon content soil and high-ring PAHs. The desorption kinetics data indicated that the method can predict the bioaccessible fraction of PAHs. In addition, the method predicted a satisfactory accumulation into earthworms (Eisenia fetida) with a slope statistically approximated to 1. A highly significant linear regression (R2 = 0.95) was also found between the proposed method and Tenax desorption in historically contaminated soils, demonstrating that the method is an efficient and convenient approach for the bioaccessibility prediction of PAHs in soils.

Human intestinal Caco-2 cell line in vitro assay to evaluate the absorption of Cd, Cu, Mn and Zn from urban environmental matrices

The Caco-2 cell line is derived from a human colon adenocarcinoma and is generally used in toxicity assays. The ingestion of soil or dust is a significant route of human exposure to potential harmful elements (PHE), and assays of bioaccessibility or bioavailability can be used to measure the potential hazard posed by exposure to toxic substances. The in vitro digestion (UBM method) and Caco-2 cell model were used to investigate the bioaccessibility and absorption by intestinal cells of the PHE in four matrices (two urban soils and two soils with lead (Pb)-mining tailings) along with the guidance material for bioaccessibility measurements, BGS 102. The gastrointestinal (GI) compartment was simulated, and the resulting material added to Caco-2 cells. In the GI, the average bioaccessibility was 24% for cadmium (Cd), 17% for copper (Cu), 0.2% for Pb, 44% for manganese (Mn) and 6% for zinc (Zn). The poor reproducibility was attributed to the pH (6.3) and the highly complex GI fluid that formed PHE precipitates and complexes. In 2h, Caco-2 cells absorbed 0.2ngmg-1 of cellular protein for Cd, 13.4ngmg-1 for Cu, 5ngmg-1 for Mn and 31.7µgmg-1 for Zn. Lead absorption was lower than the limit of quantification (< 2µgL-1). Cd was presented in the cell monolayer and could interfere in the intracellular accumulation of Cu, Mn and Zn. The use of in vitro assays allowed for an estimation of the absorption of Cd, Cu, Mn and Zn from environmental matrices to be made, and except for Mn, it had a positive correlation with bioaccessible concentration, suggesting a common association of these elements in the cellular environment.

Oral bioaccessibility and human health risk assessment of trace elements in agricultural soils impacted by acid mine drainage

Cultivated soils around the historic mine site of Tharsis (Spain) contain elevated concentrations of As (up to 621 mg kg−1), Cu (752 mg kg−1) and Pb (2395 mg kg−1), exceeding the regional background levels and the statutory limits set for agricultural use. A site-specific health risk assessment of occupational and environmental exposures was conducted using an approach based on guidelines from regulatory agencies, refined by combining bioaccessibility and bioavailability data.Oral bioaccessibility, as determined by simulating the human digestion process in vitro (Unified BARGE Method), was largely related to total trace element concentrations in soil. Arsenic seemed to be evenly distributed among the gastric and gastro-intestinal phases (about 31%), whereas the bioaccessible fraction of pH-dependent metal cations, like Pb and Zn, was noticeably higher in the stomach (nearly 50%) than in the gastro-intestinal tract (less than 10%). Bioaccessibility assessed by single extraction with 0.43 M HNO3 was overestimated by a factor of 1.2–1.4 relative to that obtained from the BARGE method.Site-specific relative bioavailability (RBA) values of As (27.7%) and Pb (42.6%), predicted from bioaccessibility measurements through linear regression models, had little effect on the overall risk estimates. For the ingestion pathway, the RBA-adjusted cancer risk values (9.7E-05 to 2.0E-04) exceeded the regulatory threshold in all plots, and the hazard index re-calculated after adjustment of oral dose was also above the allowable limit, with values ranging from 2.5 to 4.8. However, no detrimental health effects are expected to occur through inhalation of soil particles in nearby residents.

The Link between Soil Geochemistry in South-West England and Human Exposure to Soil Arsenic

The aim of this research is to use the whole soil geochemistry and selected bioaccessibility measurements, using the BioAcessibility Research Group of Europe (BARGE) method, on the same soils to identify the geochemical controls on arsenic (As) bioaccessibility and to gain an understanding of its spatial distribution in south-west England. The total element concentrations of 1154 soils were measured with As concentrations ranging from 4.7–1948 mg·kg−1, with the bioaccessible As of 50 selected soils ranging from 0.6–237 mg·kg−1. A Self Modelling Mixture Resolution approach was applied to the total soil element chemistry to identify the intrinsic soil constituents (ISCs). The ISCs were used as predictor variables and As bioaccessibility as the dependant variables in a regression model for the prediction of As bioaccessibility at all soil locations to examine its regional spatial distribution. This study has shown that bioaccessibility measurements can be directly linked to the geochemical properties of soils. In summary, it seems the primary source of bioaccessible As comes from soils developed directly over the mineralised areas surrounding the granite intrusions. Secondary sources of bioaccessible As are derived from As that has been mobilised from the primary mineralised source and then re-absorbed onto clay material, Fe oxides and carbonate coatings. This information can be of direct use for land development, since land contamination can affect the health of people living, working, visiting or otherwise present on a site.

In vitro bioaccessibility of potentially toxic metals (PTMs) in Baoji urban soil (NW China) from different functional areas and its implication for health risk assessment

In order to better understand both the conceptual and operational aspects of bioaccessibility and phytobioavailability of PTMs (Co, Cr, Cu, Li, Mn, Ni, Pb, Zn) in different urban soils, a total of 30 soil samples from agricultural region, entertainment district, education area, traffic area, residential area and industrial area (IA) in Baoji urban city (NW China) were collected and the bioaccessibility and phytobioavailability were measured by multi-in vitro models of PBET, SBET, citric acid leaching and Tessier sequential extraction procedure, respectively. The suitable in vitro measurement of bioaccessibility and phytobioavailability for each PTM was selected and would be reliably applied for health risk assessment. The results indicated that the bioaccessibility and phytobioavailability for each PTM evaluated by in vitro models depended on PTM total concentration and anthropologic activity influence. The health risks associated with bioaccessibility of PTMs exposure showed that the carcinogenic and non-carcinogenic risks in all areas for children and adults were below the threshold or acceptable risk levels except lead exposure on children in IA, indicating there were more health risks to the children in than that in other functional areas. It was worth that the highest observation of Pb in IA would strongly correlate with lead-acid battery industries, which the emissions would influence on the occurrences of Pb distributing in the other functional areas, which were supported from the analysis results of XPS. Therefore, the continuous monitoring and attention to the health risk of inhabitants in different functional areas should be paid.

Arsenic in Playground Soils from Kindergartens and Green Recreational Areas of Bratislava City (Slovakia): Occurrence and Gastric Bioaccessibility.

In this study, playground soils of kindergartens and green recreational zones in Bratislava were investigated for the occurrence and gastric bioaccessibility of arsenic (As) in the < 150μm soil size fraction. Eighty topsoil (0-10cm) samples were collected from playgrounds in kindergartens and green recreational zones throughout the urban area. Bioaccessibility measurements of As were performed using the Simple Bioaccessibility Extraction Test that mimics the human gastric environment, and resulting extracts were analyzed by hydride generation-atomic absorption spectrometry to assess bioaccessible As concentrations in the collected playground soils. Single selective chemical extractions using hydroxylamine hydrochloride-hydrochloric acid and dithionite-citrate-bicarbonate solutions also were used to determine the amount of As associated with amorphous and amorphous/crystalline Fe oxy-hydroxides in soils, respectively. The results showed that the spatial distribution of total As concentrations was related to the historical development of the city, with higher soil concentrations of As found in the old city centre and related urban zones and the lower ones on the outskirts of Bratislava. There was a variation in the values of bioaccessible concentrations and fractions of As, with ranges from 0.40 to 5.60mg/kg and 7.29 to 56.1%, respectively. Correlation and multivariable linear regression analyses revealed that bioaccessible concentrations of As were linearly related to its total concentrations in the soils, whereas dithionite-citrate-bicarbonate extractable Fe (FeDCB) was the main soil property, controlling the bioaccessibility of As. When the amount of FeDCB in the soils increased, As bioaccessibility decreased, confirming an importance of Fe bound to amorphous and crystalline iron oxy-hydroxides to the limitation of As bioaccessibility in urban playground soils of Bratislava. Additionally, single selective extractions showed that As concentrations extracted by hydroxylamine hydrochloride (AsHH) and dithionite-citrate-bicarbonate (AsDCB) were positively correlated with its bioaccessible concentrations (Spearman r = 0.75 and 0.62, respectively; p < 0.001).