Biocemented Sand Research Articles

Bio-reinforcement efficiency of enzyme-induced carbonate precipitation modified by sword bean crude urease combined with multiple enhancers on bio-cemented sand

Bio-reinforcement efficiency of enzyme-induced carbonate precipitation modified by sword bean crude urease combined with multiple enhancers on bio-cemented sand

Experimental and simulation study of calcium carbonate non-uniform distribution characteristics of bio-cemented sand

The innovative technology of microbially induced calcium carbonate precipitation (MICP) soil modification is widely used in soil reinforcement, soil remediation, environmental remediation and other fields, and has gradually become a hot research direction. Recent research is more focused on the overall physical and mechanical characteristics of the uniform reinforced specimen. There is limited research on the distribution characteristics of calcium carbonate along the seepage path during the reinforcement process. However, the distribution characteristics of calcium carbonate play a significant role in the physical and mechanical characteristics of the solid, especially in the large-scale MICP reinforcement process. In this study, based on the microbial mineralization reaction tests of different concentrations of cement solution at the laboratory sample scale, the calcium carbonate distribution on the surface of the sample at the microscale SEM observation and the CT scanning reconstruction of the micro pore structure characteristics of the microbial reinforced soil, we discussed the characteristics of the non-uniform reduction of calcium carbonate distribution and porosity in the process of microbial consolidation. Based on the Kozeny-Carman equation, the conceptual model of effective porosity was introduced, and the time relationship between porosity and calcium carbonate precipitation was considered to establish a bio-chemical-seepage multi-physics field coupling model. The precipitation of calcium carbonate in the axial direction of the model sample exhibits an uneven feature of more at the top and less at the bottom, and the distribution characteristics of porosity also show an uneven feature of more at the top and less at the bottom.

Influence of shear strength parameters on loose fine sand treated with coir fiber and microbially induced calcite precipitation

In recent years, there has been an increasing use of Microbially Induced Calcite Precipitation (MICP) technology in ground improvement. Adding fiber to bio-cemented sand leads to significant improvements in its properties. This paper examines how coir fiber and microbially induced calcite precipitation (MICP) might be used to improve the engineering qualities of fine sand. The sand was mixed with Coir fiber at varying Fiber Contents (FC) of 0.1%, 0.2%, 0.3%, 0.4%, and 0.5% by weight of sand. Additionally, varied Aspect Ratios (AR) of 45, 90, 136, 182, and 227 were considered. The mixture also included urease-producing bacteria (Sporosarcina Pasteurii), as well as solutions of urea and calcium chloride with 3M molarity. The untreated and treated sand samples were submitted to a Direct Shear Test (DST). The study found that the shear strength parameters, such as the angle of internal friction, increased by 1.1 times compared to standard fine sand. Additionally, the angle of internal friction (ϕ) of fiber-reinforced sand with MICP showed 1.4 times increase, while the cohesion values demonstrated a sixfold increase compared to MICP fine sand. It was determined that the optimal aspect ratio was 182, and the optimal fiber content was 0.3%. Moreover, the Scanning Electron Microscope (SEM) was utilized to examine the calcite formations present in the treated sand samples, and calcite precipitation was observed between the pores of the soils.

DEM investigation into the small-strain stiffness of bio-cemented soils

AbstractBio-mediated methods, such as microbially induced carbonate precipitation, are promising techniques for soil stabilisation. However, uncertainty about the spatial distribution of the minerals formed and the mechanical improvements impedes bio-mediated methods from being translated widely into practice. To bolster confidence in bio-treatment, non-destructive characterisation is desired. Seismic methods offer the possibility to monitor the effectiveness and mechanical efficiency of bio-treatment both in the laboratory and in the field. To aid the interpretation of shear wave velocity measurements, this study uses the discrete element method to examine the small-strain stiffness of bio-cemented sands. Bio-cemented specimens with different characteristics, including properties of the host sand (void ratio, uniformity of particle size distribution) and properties of the precipitated minerals (distribution pattern, content, Young’s modulus), are modelled and subjected to static probing. The mechanisms affecting the small-strain properties of cemented soils are investigated from microscopic observations. The results identify two mechanisms controlling the mechanical reinforcement associated with bio-cementation, namely the number of effective bonds and the ability of a single bond to improve stiffness. The results show that the dominant mechanism varies with the properties of the host sand. These results support the use of seismic measurements to assess the mechanical efficiency and effectiveness of bio-mediated treatment.

Sand improvement by microbial-induced carbonate precipitation with casein micelles

This study explores the incorporation of casein micelles to improve the conventional microbial-induced carbonate precipitation technique used for soil improvement. With a single application of the proposed biocementing agent containing casein micelles, a mixed slurry of natural sand solidified to self-sustaining strength within 48 h at 37°C was obtained. The stress–strain curves of the biocemented specimens obtained from a consolidated drained triaxial compression test exhibited sharp peaks at less than 1% strain. Under effective confining pressures of 30, 50 and 100 kPa, the peak deviatoric stress registered 415, 479 and 536 kPa, respectively – representing 1·8–4·1 times the maximum strength of the original sand. Vaterite calcium carbonate crystals formed by the biocementing agent ensured bridging between sand particles, resulting in the improved cohesion. The cohesion-derived residual strength tended to increase at the critical state in biocemented sand under the lowest confining stress. The localised shear band formed by the crystals may control the movement of sand particles, resulting in a change in volumetric strain behaviour. The formation of casein micelles induced the nucleation and growth of spherical crystals, acting as a casein-mediated vaterite. This formation likely contributed to the improved cohesion of sand observed in this study.

Effect of sticky rice on the strength and permeability of bio-cemented sand

Microbially induced carbonate precipitation (MICP) is an eco-friendly soil improvement technique. However, this method still has some drawbacks, such as low conversion efficiency of CaCO3 crystallization, insufficient strength for certain applications, and requiring multiple treatments. Previous studies have reported that sticky rice can regulate CaCO3 crystals (i.e., chemical CaCO3) in the sticky rice-lime mortar, showing potential for improving the bio-cementation. Therefore, this study explored the possibility of using sticky rice to enhance the biocementation effect. Tests were carried out to assess the strength and permeability of bio-cemented sand with the inclusion of sticky rice. The results indicated that sticky rice may regulate the type and size of bio-CaCO3 crystals, and the use of an appropriate amount of sticky rice as additive could increase the strength of sand columns by regulating CaCO3 crystallization. Polyhedral calcites may be more favourable for the increasing strength than some vaterites with a hollow spherical structure. The combination of MICP and sticky rice can significantly decrease the coefficient of permeability to a value that was much lower than that by using sticky rice and MICP alone. Bio-CaCO3 immobilized the sticky rice on one end on sand particles, and the reticulated structure of sticky rice divided large pores into small pores, which may be the important cause of the decrease in permeability coefficient. Finally, this study proposed that the MICP with the sticky rice as an additive may enhance the MICP effect and prevent the surface erosion of coarse-grained sand slopes.

Effects of bacterial strains on undrained cyclic behavior of bio-cemented sand considering wetting and drying cycles

The microbial-induced calcite precipitation (MICP) technique has been developed as a sustainable methodology for the improvement of the engineering characteristics of sandy soils. However, the efficiency of MICP-treated sand has not been well established in the literature considering cyclic loading under undrained conditions. Furthermore, the efficacy of different bacterial strains in enhancing the cyclic properties of MICP-treated sand has not been sufficiently documented. Moreover, the effect of wetting-drying (WD) cycles on the cyclic characteristics of MICP-treated sand is not readily available, which may contribute to the limited adoption of MICP treatment in field applications. In this study, strain-controlled consolidated undrained (CU) cyclic triaxial testing was conducted to evaluate the effects of MICP treatment on standard Ennore sand from India with two bacterial strains: Sporosarcina pasteurii and Bacillus subtilis. The treatment durations of 7 d and 14 d were considered, with an interval of 12 h between treatments. The cyclic characteristics, such as the shear modulus and damping ratio, of the MICP-treated sand with the different bacterial strains have been estimated and compared. Furthermore, the effect of WD cycles on the cyclic characteristics of MICP-treated sand has been evaluated considering 5–15 cycles and aging of samples up to three months. The findings of this study may be helpful in assessing the cyclic characteristics of MICP-treated sand, considering the influence of different bacterial strains, treatment duration, and WD cycles.

Effect of Fines Content on Calcium Carbonate Precipitation and Thermal Properties of Biocemented Sand

Effect of Fines Content on Calcium Carbonate Precipitation and Thermal Properties of Biocemented Sand

Discrete element simulations to predict the response of bio-cemented sands

Discrete element method (DEM)-based numerical models in the YADE environment are used to simulate the constitutive response of uncemented and bio-cemented sands to investigate the influence of boundary conditions, loading and testing conditions, and material types. Both the classical DEM model and the pore scale finite volume (PFV)-coupled DEM model are used to simulate the response of saturated uncemented and lightly cemented sands with a rigid wall boundary under both drained and undrained triaxial compression. A DEM model with flexible boundaries created using particle facet (PFacet) elements is used to simulate undrained triaxial compression of moderately cemented sands, including the influence of confining stress. The PFacet-based model is used to predict the transition from barreling failure to shear banding when the confining stress or the cementation degree increases. The classical DEM model with cohesive bonds of uniform strength is also used to successfully simulate the uniaxial compression response of a sand with an extremely high degree of cementation. Finally, this paper presents a particle-packing model consisting of multiple solid phases for cemented sands based on the understanding that not all particle types will have the same cohesive properties. This multiple solid-phase model is a refinement of the classical DEM model that represents the particle physics more realistically, especially for heterogeneous systems. A preliminary parametric study is carried out considering varying cohesive properties and volume fractions for the different solid phases.

Tensile Strengths and Size Effects of Biocemented Sands

Tensile Strengths and Size Effects of Biocemented Sands

Micromechanics of multiphase geomaterials based on micro-CT image analysis: unsaturated soil, vegetated soil, and bio-cementation

Under the influence of climate change, extreme weather events such as rainstorms may induce fluctuations in soil water content above the groundwater level, thereby causing geological disasters (e.g., landslides). To mitigate these hazards, green and low-carbon engineering measures such as vegetation reinforcement and bio-cementation are proposed. This study investigates the micromechanical properties of multiphase geomaterials—unsaturated soil, vegetation-reinforced sand, and bio-cemented sand—utilizing high-resolution computed tomography (CT) scanning technology and associated image analysis techniques. The findings are presented as follows: (1) a 4D examination of the macroscopic and microscopic behaviors of unsaturated granular materials under triaxial loading conditions was conducted using self-designed in situ CT scanning equipment. (2) Triaxial loading alters the distribution of phase interfacial surfaces in unsaturated soil, affecting its overall strength. This loading also increases the anisotropy of the solid skeleton and suction stress. (3) The global saturation of the root–soil complex diminishes with root growth, with pore distribution significantly influencing local saturation. (4) In the unsaturated MICP process, low-saturation conditions are preferable for effective cementation, although the saturation levels should be restricted to 20%. After calcification, the particle contact coordination number increases, maintaining the isotropy in the contact between the soil particles.

Applicability of electrical resistivity measurements for monitoring biocementation treatment in soils

In this paper the use of ER measurements in soils in the context of biocementation, is investigated considering its use as an indicator of treatment efficiency measured trough the presence of biocement, or to provide information about the presence of chemical by-products during or after final washing operations. The electrical resistivity of a slightly biocemented sand was analyzed on a laboratory scale test conceived to reproduce field measurements conditions such as varying degrees of saturation and the presence of chemical by-products in the pore fluid. The results confirmed that, independently from the degree of saturation, the nature of the pore fluid was the parameter that affected most the electrical resistivity measurements, and the presence of calcium carbonate affecting soil structure was detected only after removal of the treatment by-products by a complete final soil washing using water. From a practical point of view this non-destructive technique is more adequate to be used mainly at the end of the treatment, to check that complete soil washing has occurred, rather than checking the presence of bio-cement specially if the amount precipitated is not very high as it was the case of the tests performed.

Identification and extraction of cementation patterns in sand modified by MICP: New insights at the pore scale.

Microbially induced calcium carbonate precipitation (MICP) is an environmentally friendly technology that improves soil permeability resistance through biocementation. In this study, 2D microscopic analysis and 3D volume reconstruction were performed on river sand after 24 cycles of bio-treatment based on stacked images and computed tomography (CT) scanning data, respectively, to extract biocementation patterns between particles. Based on the mutual validation findings of the two techniques, three patterns in the biocemented sand were identified as G-C-G, G-C, and G-G. Specifically, 2D microscopic analysis showed that G-C-G featured multi-particle encapsulation and bridging, with a pore filling ratio of 81.2%; G-C was characterized by locally coated particle layers, with a pore filling ratio of 19.7%; and the G-G was marked by sporadic filling of interparticle pores, with a pore filling ratio of 11.7%. G-C-G had the best cementation effect and permeability resistance (effective sealing rate of 68.5%), whereas G-C (effective sealing rate of 2.4%) had a relatively minor contribution to pore-filling and flow sealing. 3D volume reconstruction showed that G-C-G had the highest pore filling rate, followed by G-G and G-C. The average filling ratios of area and volume for G-C-G were 83.979% and 77.257%, respectively; for G-G 20.360% and 23.600%; and for G-C 11.545% and 11.250%. The analysis of the representative element volume (REV) was conducted, and the feasibility and reliability of the micro-scale pattern extraction results were confirmed to guide the analysis of macro-scale characteristics. The exploration of the effectiveness of cementation patterns in fluid sealing provides valuable insights into effective biocementation at the pore scale of porous media, which may inspire future research.

Influence of the coefficient of uniformity on bio-cemented sands: A microscale investigation

Microbially induced carbonate precipitation (MICP) is a bio-mediated ground improvement technique that can increase soil stiffness and produce cohesion within granular material. Most experimental investigations on MICP-treated soils are performed on idealized granular materials. Evaluating a narrow range of particle sizes dismisses the potential influence of soil fabric on MICP treatment efficiency. Therefore, little is known regarding the influence of soil fabric on the level of improvement achievable post-MICP treatment. We investigate the influence of the coefficient of uniformity (Cu) on the level of improvement that can be obtained from MICP treatment. This study couples unconfined compression testing with microscale observations obtained from x-ray computed tomography (CT) of two sand mixtures with different Cu values. A soil column and CT specimen of each sand mixture were prepared and received the same number of MICPinjections. The shear wave velocity (Vs) of the soil columns was monitored to evaluate the increase in soil stiffness over time. After MICP treatment, the bio-cemented columns were subjected to unconfined compressive strength testing. Results indicate that for a similar mass of carbonate, the soil with a larger Cu experienced a greater increase in Vs but a lower maximum unconfined compressive strength. Through CT imaging, the soil with a smaller Cu was observed to have a more uniform distribution of carbonate within the sand matrix whereas the soil with a larger Cu has more sporadic MICP trends. This study elucidates the influence of soil fabric on the level of improvement that can be achieved through MICP treatment and assesses the reliability of x-ray CT scanning of MICP-treated sands with moderate carbonate content.

Towards a monitoring tool to quantify urease during biocementation treatment at microscale

Biocementation consists in using urease enzyme and a solution rich in urea and in calcium to precipitate calcium carbonate (biocement). When applying this treatment in soils, the biocement minerals bond the grains improving overall soil’s hydro-mechanical properties. For the practical use of this technique, it is necessary to be able to predict the properties of the treated soil after following specific protocols, by preference avoiding non-destructive testing such as those performed on samples extracted after the treatment. The amount of biocement precipitated depends on the amount of urease enzyme, urea and calcium. This idea has inspired the development of one magnetoresistive biosensor to detect urease, to be used as a non-destructive monitoring tool during the treatment. A magnetoresistive platform was used to quantify the signal, which is related to the urease concentration through a calibration curve. The sensor was tested to measure the enzyme present in the inflow and outflow fluids used to treat cylindrical soil samples (2.5 cm diameter and 2.0 cm height), prepared with a uniform grading size sand (D50=0.3 mm). Purified urease from Canavalia ensiformis, was used. The improvement of the biocemented sand samples was quantified through measuring the calcium carbonate content of the soil after the treatment and the values were related with the amount of enzyme retained by the soil, determined using the sensor readings. This work found, for the first time, the relationship between the measured concentration of urease retained by soil and the calcium carbonate content precipitated. This relationship is an important tool for monitoring the treatment, without the need to use destructive tests or even stop the treatment.

Shear wave velocity and its anisotropy of biocemented glass sand

The paper presents a series of experimental studies on the anisotropic small strain stiffness of a biocemented glass sand. Multidirectional shear wave velocities were measured on the isotropic consolidated samples in a triaxial cell. The biotreatment method, shear wave velocity, and its anisotropy of the biocemented sand were reported. It is found that the shear wave velocity increases with the increase of cementation level and the stiffness anisotropy firstly increases then decreases with the increase of cementation level. The paper aims to provide a new way to simulate the undisturbed state of sandy soils in laboratory with the microbially induced calcite precipitation technique.

Micro-macro investigation on bio-cemented sand under different grouting saturation: An effective enhancement method

Microbial-induced calcium carbonate precipitation (MICP) is a new biotechnology that can be used to improve the strength of soils. Unsaturated soils are common in nature and saturation is a significant factor affecting the efficiency of bio-cementation. This study investigated the properties of MICP under different grouting saturation conditions. Unconfined compressive strength (UCS) tests confirmed that biocemented sand could get higher strength under unsaturated grouting conditions with the same calcium carbonate content which helps reduce the material cost. Scanning electron microscopy (SEM) test results show that at lower saturation, the size and amount of calcium carbonate crystals were insufficient but calcium carbonate mainly gathered between the particles. At higher saturation, larger calcium carbonate crystals were produced and exited in pores and on the particle surface, increasing the filling effect. Energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD) test results show that the dominant calcium carbonate morphology detected in samples was calcite, which was the most stable one. X-ray computed tomography (CT) test results show that after cementation, the measured contact surface area became uniform and the coordination number was higher. The flow direction of bacteria and the cementing solution did not induce significant anisotropy in the cementation process. The effective cementation and content of calcium carbonate jointly influenced the improvement of soil mechanical properties.

Experimental study on improving hydraulic characteristics of sand via microbially induced calcium carbonate precipitation

Microbially induced calcium carbonate precipitation (MICP) technology has garnered significant attention for enhancing soil engineering properties, presenting a potential alternative to traditional cementitious materials for soil seepage control. This study investigates the application of MICP to enhance the hydraulic characteristics, specifically reducing porosity and hydraulic conductivity, of loose sandy soils. Three types of sand-river sand, sea sand, and quartz sand-underwent MICP treatment in cylindrical molds using multiple treatment schemes. Laboratory experiments, including permeability tests, porosity tests, scouring and soaking resistance tests, microstructural testing and analysis, and microfluidic chip tests, were conducted to evaluate the hydraulic characteristics and microstructure contributing to sealing. The results revealed that the structural integrity of the MICP-treated sand declined with an increase in cementation solution (CS) concentration, which were then categorized into intact, discontinuous, and loose blocks. The average decreases in porosity and hydraulic conductivity were 5.5% and 97.2%, respectively, from 0.382 and 4.33 × 10-4 m/s (before treatment) to 0.361 and 1.2 × 10-5 m/s (after treatment). Three cementation patterns, G-C-G, G-G, and G-C, were identified in the MICP-treated sand, with corresponding pore-filling rates decreasing successively. Furthermore, the study explores the feasibility of individually distinguishing and characterizing the contributions of biofilms and calcium carbonate precipitation to the reduction in porosity and permeability in biocemented sand through simulation.

Closure to “Axisymmetric Simulations of Cone Penetration in Biocemented Sands”

Closure to “Axisymmetric Simulations of Cone Penetration in Biocemented Sands”

Discussion of “Axisymmetric Simulations of Cone Penetration in Biocemented Sands”

Discussion of “Axisymmetric Simulations of Cone Penetration in Biocemented Sands”