Succinic acid is a biobased chemical which exists in plants, humans, microorganisms, etc. It is considered as a building block chemical due to its wide application in many industries such as pharmaceuticals, food, chemicals, polymers, cosmetics, agriculture, etc. Succinic acid can be manufactured through fermentation and chemical synthesis process. Separation of succinic acid from fermentation broth is expensive, complicated, and non-environment friendly. In present study, rice bran, mustard, and soybean oil were used as solvents to separate succinic acid from the aqueous phase. Experiments were carried out using 0.111–0.513 mol.L −1 of succinic acid, and the results were defined as distribution coefficient (K D ) and separation efficiency (E). The maximum separation efficiency of 15.20, 10.82, and 9.36% was obtained at 0.111 mol.L −1 of aqueous phase with rice bran, mustard, and soybean oil, respectively. This study can help to reduce the toxicity of the separation of succinic acid from fermentation broth. Also, efficiency may be increased by using reactive extractants.