Bile Acid Binding Activity Research Articles

Probiotic Gac fruit beverage fermented with Lactobacillus paracasei: Physiochemical properties, phytochemicals, antioxidant activities, functional properties, and volatile flavor compounds

This study aimed to develop a probiotic beverage from Gac juice (GJ) at 5–10 g/100 g fermented with L. paracasei CASEI 431 at 37° for 24 h. Probiotic viability, physicochemical properties, phytochemicals, antioxidant activity, volatile flavor compounds, and sensory acceptability of fermented GJ were investigated. A decrease in pH value and sugar content with an increase in organic acids (lactic and acetic acid) and probiotic viability were detected after GJ fermentation. Interestingly, the β-carotene content, DPPH radical scavenging activity, ferric reducing antioxidant power, and lipid peroxidation inhibition capacity of fermented GJ were all higher than those of non-fermented GJ. Furthermore, fermented GJ at 10 g/100 g showed a remarkable ability to disrupt cholesterol micellization formation and increase bile acid binding activity. The fermentation of GJ introduced various volatile compounds without affecting sensory acceptability. The findings suggest that GJ might be a promising ingredient for developing a non-dairy probiotic beverage with increased bioactive phytochemicals and functional properties.

In vitro binding capacities, physicochemical properties and structural characteristics of polysaccharides fractionated from Passiflora edulis peel

This study aimed to determine structural characteristics, physical properties and binding effects of polysaccharide and its fractions from Passiflora edulis peel on lipid, cholesterol and bile acids. The crude polysaccharide (WPEP) was extracted using hot water extraction method, fractionated using DEAE-52 cellulose column, and purified by Sepharose CL-6B column to obtain four polysaccharide fractions (WPEP-F1, WPEP-F2, WPEP-F3, and WPEP-F4). The physicochemical properties and structural characteristics were analyzed using several methods. The chemical-binding effects were also determined. Among all polysaccharide samples, WPEP-F2 had the highest total sugar content (77.63%). The viscosity of all polysaccharide samples was increased. Among the four polysaccharide fractions, WPEP-F2 showed the best emulsifying activity. WPEP-F1 was mainly composed of glucose, galactose, mannose, and arabinose, whereas WPEP-F2, WPEP-F3, and WPEP-F4 were principally composed of galacturonic acid. SEM analysis showed that the four fractions had different microstructures. Among the polysaccharide components, WPEP-F2 had the highest lipid-binding effect (9.3 g/g). It also had the best binding effect on taurocholic acid, deoxycholic acid, glycocholic acid, and cholesterol (50.87%, 69.12%, 30.22%, and 78.68%, respectively), but WPEP-F3 had the best binding effect on cholic acid (53.99%). The lipid, cholesterol, and bile acid-binding activities of WPEP-F2 could be attributed to its high viscosity, porous appearance structure, and sugar content in addition to its triple helix structure. The findings lay a theoretical foundation for developing Passiflora edulis peel polysaccharide-related foods and promoting application of the peel polysaccharide in functional food industries.

Isolation and characterization of a novel protein from Momordica charantia L. Positively regulates lipid metabolism activity in vivo and in vitro

• MC3-2, A novel anti-hypertriglyceridemic protein was isolated from Momordica charantia L.. • MC3-2 binded to bile acids, and decreased FFA-induced HepG2 steatosis and intracellular TG. • MC3-2 regulated high-fat-diet hypertriglyceridemia in vivo tests. • MC3-2 ameliorated inflammatory cytokines and oxidative stress by regulating related protein. In this study, a novel protein designated MC-2 was isolated from Momordica charantia L. , and the positively regulates lipid metabolism activity was detected by the bile acid binding activity test. Then, the amino acid composition of MC-2 was analyzed, and the total content of Asp, Glu, and Lys is up to 38.96 g/100 g in MC-2. Furtherly, its lipid metabolism activity were explored. In vitro, MC-2 ameliorated FFA-induced HepG2 steatosis phenotypes and intracellular triglycerides level with the promotion of bile acids secretion. In vivo, MC-2 was able to protect rats against NAFLD-induced in response to a high-fat diet through a manner associated with the regulation of inflammatory cytokines and oxidative stress-related factors. The hepatoprotective activity of MC-2 was closely tied to improvements CPT1A, FATP2, ACSL1, and PPAR-α expressions. Together, these data highlight the promise of MC-2 (rich in Asp, Glu, and Lys) as a candidate functional food ingredient.

In vitro and in vivo evaluation of the bile acid-binding properties of dried persimmon and its non-extractable proanthocyanidin fraction

Dried persimmons contain abundant insoluble tannins comprised of non-extractable proanthocyanidins (NEPAs). To determine the beneficial function of NEPAs, we studied the bile acid-binding ability of NEPAs using in vitro and in vivo experiments. For the in vitro evaluation, 70% (v/v) acetone-insoluble solids (AIS) with different NEPA levels was prepared from persimmon fruits during drying. AIS with higher NEPA levels displayed stronger bile acid-binding activity, and the highest NEPA levels can be obtained from mostly-dried fruits. The in vivo bile acid-excreting effect of dried persimmon powder (DP) and AIS was verified by feeding experiments using C57BL/6J mice. The average bile acid-excretion was in order of low-fat group (1.07 µmol/day) < high-fat (HF) group (3.03 µmol/day) < HF + AIS group (4.44 µmol/day) < HF + DP group (6.74 µmol/day). These results suggest that the bile acid-excreting function of the fruit is related to the presence of NEPAs, which are constitutive ingredients of dried persimmon.

Anti-degranulation and bile acid-binding activity of extracts from fruits and agro-industrial by-products

To evaluate the utility of fruits and by-products/wastes as bioactive materials, we investigated the anti-allergic effects of hot-water and ethanol extracts from various fruit by-products and medicinal fruits using a β-hexosaminidase release assay. Bile acid-binding ability, which is related to the acceleration of bile acid excretion, was also determined for hot-water extracts. Among the extracts, the ethanol extract of grape bunch stem showed the highest (63.6%) inhibition of degranulation, as assessed by β-hexosaminidase release from rat basophilic leukemia cells (RBL-2H3) at a dose of 100 μg/mL. The hot-water extract from grape seed contained the highest total polyphenol content (353 mg gallic acid equivalent/g dry extract), but the total polyphenol content did not show a high correlation with the anti-degranulation effect. A positive relationship between the inhibitory effect of degranulation and proanthocyanidin content (0.1–0.3 in A550) was observed, but higher concentrations of proanthocyanidins in the system showed adverse effects. Proanthocyanidin content was also related to the bile acid-binding ability of the hot-water extracts with the highest value from Chinese quince hot-water extract (95%, 47.7 mg/g dry extract). These results suggest that proanthocyanidin-rich extracts from fruits and agro-industrial by-products have bioactive potential and can be exploited as anti-degranulation and bile acid-eliminating materials.

Resistant starch type 2 from lotus stem: Ultrasonic effect on physical and nutraceutical properties

Resistant starch type 2 (RS) was isolated from lotus stem using enzymatic digestion method. The isolated RS was subjected to ultrasonication (US) at different sonication power (100–400 W). The US treated and untreated RS samples were characterized using dynamic light scattering (DLS), scanning electron microscopy (SEM), light microscopy and Fourier transform infrared spectroscopy (FT-IR). DLS revealed that particle size of RS decreased from 12.80 µm to 413.19 nm and zeta potential increased from −12.34 mV to −26.09 mV with the increase in sonication power. SEM revealed smaller, disintegrated and irregular shaped RS particles after ultrasonication. FT-IR showed the decreased the band intensity at 995 cm−1 and 1047 cm−1 signifying that US treatment decreased the crystallinity of RS and increased its amorphous character. The bile acid binding, anti-oxidant and pancreatic lipase inhibition activity of samples also increased significantly (p < 0.05) with the increase in sonication power. Increase in US power however increased the values of hydrolysis from 23.11 ± 1.09 to 36.06 ± 0.13% and gylcemic index from 52.39 ± 0.38 to 59.50 ± 0.11. Overall, the non-thermal process of ultrasonic treatment can be used to change the structural, morphological and nutraceutical profile of lotus stem resistant starch which can have great food and pharamaceutical applications.

In vitro lipid-lowering properties of the fruits of two bignay [Antidesma bunius (L.) Spreng] cultivars as affected by maturity stage and thermal processing

Bignay [Antidesma bunius (L). Spreng] fruit contains an array of polyphenols and information on how these bioactive compounds vary with cultivar type, maturity stage, and process treatment are unclear. Also, the effects of these variations on the lipid-lowering potential of this Philippine indigenous berry have not been reported. This study aimed to evaluate the lipid-lowering properties of the fruits of two bignay cultivars as affected by maturity stage and thermal processing. In vitro lipid-lowering assays revealed that both bignay cultivars had appreciable pancreatic lipase inhibitory activity, bile acid binding capacity, and cholesterol micellar solubility inhibition, which were comparable to those of the known lipid-lowering agents used as positive controls in this study. Freeze-dried samples of the freshly harvested fruits of both bignay cultivars [i.e., Common Cultivar (CC) and Kalabaw cultivar (KC)] had the highest bile acid binding activity (41.9–45.5% for CC and 43.4–54.0% for KC) for all the three maturity stages implying the beneficial effects of fresh bignay fruits related to lipid metabolism. Steam-blanched fruits had the highest pancreatic lipase inhibition activity (17.8–37.4% for CC and 29.2–39.0% for KC), regardless of maturity stage, while water-blanched samples exhibited the highest cholesterol micellar solubility inhibition (39.6–42.2% for CC and 40.2–47.6% for KC). Thermal processing tended to lower the lipid-lowering properties of the bignay fruits relative to their freeze-dried fresh fruits. Results of this study showed the potential of Philippine bignay fruit as a functional food that may be helpful in the management of dyslipidemia.

Functional characterization of basil (Ocimum basilicum L.) seed mucilage

Basil seed mucilage (BSM- 20.49%) was extracted from basil seeds to investigate its functional, thermal, rheological, and morphological properties. BSM was purified into basil seed gum (BSG- 18.75%) and further fractionated into alcohol soluble fraction (F1- 6.80%), alcohol insoluble fraction (F2- 13.70%), and protein free fraction (F3- 18.50%). Total dietary fibre content observed for basil seed mucilage was 98.50%. Analysis of functional properties showed higher values for water absorption capacity (35.16–38.96 g/g), and emulsification capacity (101–115.55 g/g). However, lower values were found for protein solubility (0.06–0.59%), oil absorption capacity (5.40–17.38%), foaming capacity (41.42–50%) and syneresis (1.27%). Hydration of mucilage in salt solutions (KCl, NaCl, and CaCl2) revealed that salts sufficiently reduced the hydration capacity. Cholesterol binding (3.49–3.56%), bile-acid binding (5.85–57.35%) and α-amylase inhibitory activity (55.20–55.34%), were also investigated. Thermal measurements involved the study of glass transition temperature (Tg) by differential scanning calorimetry (DSC). Results for Tg indicated stability at high temperatures. Values for Tg and heat capacity were in the range of 122.5–148.3 °C and 9.44–29.89 J/g, respectively. Samples were analysed for visometry using rapid visco analyzer (RVA). Results showed a final viscosity of 190–714 cP. Rheological measurements showed shear thinning behaviour and an increase in storage and loss modulus on increasing angular frequency. Scanning electron micrographs showed a strong fibril-network which depicted gel-like characteristics.

In vitro bile acid binding activity and non-extractable polyphenols of dried persimmon and quince fruits

In vitro bile acid binding activity and non-extractable polyphenols of dried persimmon and quince fruits

Bile acid-retention by native and modified oat and barley β-glucan

Foods rich in cereal β-glucan are efficient dietary tools to help reduce serum cholesterol levels and hence the risk of cardiovascular diseases. However, β-glucan undergoes various reactions during food processing, which alter its viscous properties and interactions with components of the gastrointestinal tract. It has been proposed in the literature that oxidation and partial hydrolysis increase β-glucan’s bile acid-binding activity, and therefore its effectiveness in lowering cholesterol. Here, the passage kinetics of a bile salt mix across a dialysis membrane was studied with or without oat and barley β-glucan extracts, native or modified (partial hydrolysis and oxidations by sodium periodate or TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl)). Bile acid-retention turned out to be purely a function of viscosity, with the most viscous native extracts exhibiting the strongest retardation of bile acid permeation. Opposite of what was suggested in the literature, oxidation and molecular weight reduction do not seem to increase the bile acid-binding capability of β-glucan.

Ability of chicken protein hydrolysate to lower serum cholesterol through its bile acid binding activity

ABSTRACT In this study, chicken breast meat was hydrolyzed enzymatically to obtain chicken meat hydrolysate with its molecular weight to a large extent (98%) less than 3000 Da. The hydrolysate was capable of binding bile acids dose-dependently. The potential hypolipidemic effect of this hydrolysate was evaluated in high-fat-fed hamsters. Total free amino acids in the hydrolysate were seven times higher than those in the chicken meat. As compared with high-fat control, the inclusion of hydrolysate resulted in decreased concentrations of serum TG (by 16–34%) and TC (by 6–20%). The animals fed hydrolysate at a high dose exhibited 30% lower serum LDL-C levels and maintained a higher ratio of HDL-C/LDL-C (0.54) than those fed casein (0.36). Moreover, higher levels of hydrolysate inclusion were associated with increased outputs of fecal bile acid and total lipid, suggesting an association between hypocholesterolemic potential and bile acid binding capacity of the chicken hydrolysate.

Hypolipidemic effects of protein hydrolysates from Trachinotus ovatus and identification of peptides implied in bile acid-binding activity using LC-ESI-Q-TOF-MS/MS.

In the present work, analysis of the hypolipidemic properties of Trachinotus ovatus protein hydrolysates (TOPHs) and identification of peptides with bile acid-binding activity were performed. Hydrolysates prepared by trypsin digestion exhibited the highest in vitro bile acid-binding capacities compared with hydrolysates prepared with the other four proteases and were mainly composed of small peptides and amino acids with molecular weights <3 kDa, accounting for 77.30%. Among the five ultra-filtration fractions of TOPHs, TOPHs-5 (<3 kDa) exhibited the highest in vitro bile acid-binding capacity, which was equivalent to 77.97% of cholestyramine at the same concentration. A total of 68 peptides were identified from TOPHs-5 by LC-ESI-Q-TOF-MS/MS and 9 of them had hydrophobicity of more than 60%. These highly hydrophobic peptides might be associated with the bile acid-binding activity of TOPHs-5. In vivo experiments indicated that the TOPHs could effectively reduce total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and the atherogenic index (AI), while they could evidently increase the high-density lipoprotein cholesterol (HDL-C) content. Furthermore, TOPHs exerted a marked protective effect on hepatorenal function, as evidenced by decreased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatinine (CREA). Histopathological studies confirmed that TOPHs evidently protected the liver from histological alterations. In summary, for the first time, hypolipidemic effects and subsequential identification were obtained from TOPHs, which are promising natural ingredients that could potentially be employed in the management of hyperlipidemia.

Non-extractable polyphenols and in vitro bile acid-binding capacity of dried persimmon (Diospyros kaki) fruit

Dried persimmon is thought to be a rich source of non-extractable polyphenols (NEPPs). Here, we analyzed the NEPP content of dried persimmon and its bile acid-binding activity in vitro. To determine total NEPP content and epicatechin equivalent (ECE) of intact NEPPs, acid hydrolysis and non-destructive methods with 70% (v/v) acetone-insoluble solids (AIS), respectively, were conducted using Folin-Ciocalteu reagent. The ECE of intact NEPPs exceeded 1000 mg/100 g in the non-destructive method, but total NEPP content was approximately 3900 mg/100 g with the acid hydrolysis method. Thiolysis showed that the non-extractable proanthocyanidins in dried persimmon mainly comprised epigallocatechin-gallate, epigallocatechin, epicatechin, and epicatechin-gallate. AIS from dried persimmon showed stronger bile acid-binding activity than AIS from apple, quince, and fresh astringent persimmon. These results suggest that the high content of NEPPs in dried persimmon may contribute to a strong bile acid-binding activity.

Polysaccharides-protein interaction of psyllium and whey protein with their texture and bile acid binding activity

Dietary fiber is a group of important food polysaccharides, which may improve gastrointestinal function and alter the bioavailability and nutritional value of other food components. The interaction mechanism of psyllium fiber (PSY) and whey protein (WP) and the influence on the characteristics of polysaccharides and protein were investigated. The results of circular dichroism spectra and differential scanning calorimetry analysis showed that PSY could protect WP by increasing its melting temperature. The textural profiles implied that WP could improve the edibility of the psyllium fiber. The IR spectrum and colorimetric assay indicated the product of Maillard reaction between psyllium and whey protein with the heating condition. The SEM showed that the structure of the mixture became stronger with higher temperature. Furthermore, the mixture with the ratio of WP/PSY as 1:2 had three times of the bile acid binding ability of PSY, which could be developed as a nutraceutical additive with prevention of hyperlipidemia.

Insoluble Fraction from Brewer's Spent Grain reduces hypercholesterolemia in Mice fed a Western style Diet

BackgroundHypercholesterolemia and obesity are major risk factors for cardiovascular &amp; metabolic diseases. Dietary fibers offer several health benefits like improvement of the serum lipid profile, glucose tolerance, immune functions and promote weight loss. Brewer's Spent Grain (BSG) is most abundant byproduct of industrial brewing. INS is an insoluble fraction from BSG, consists of lignin 40%, arabinoxylan 25% and glucan 11%. The effect of INS was studied in Western style diet (high fat and &lt;0.05% fiber) fed mice in terms of metabolic diseases.MethodsMale mice (C57BL6) were fed with Western style diet (WD, 60% Kcal fat, &lt;0.05% fiber) ± 20% INS, cellulose or their combination (INS‐Cellulose 1:1) or a control diet (10% Kcal Fat) for 14 weeks. Body weight and food intake were monitored during the entire feeding trial. Insulin sensitivity after 12 weeks, and fasting plasma cholesterol, triglycerides and IL‐6 were measured after 14 weeks of feeding. The cecal microbiota and gene expression in liver tissue were analyzed, and fecal bile acid were measured. Histological examination and computer‐assisted morphometry of liver was performed to access hepatosteatosisResultsINS, cellulose and their combination feeding significantly reduced body weight gain (p&lt;0.05) (12.1±1.4g 13±0.9g, 11.9±0.9g vs WD 16.7+1.1 g) respectively, while there were no significant changes observed in food intake. INS and INS‐cellulose feeding significantly (p&lt;0.01) reduced total plasma cholesterol by 15% and 12%, respectively but no significant change in plasma triglyceride. INS did not improve insulin resistance; in contrast cellulose and INS‐cellulose significantly (p&lt;0.05) ameliorated it. Fecal bile acid excretion was significantly (p&lt;0.01) increased in all fiber fed up to 250% compared to WD fed mice. Plasma IL‐6 was significantly decreased with INS and cellulose as compared to WD. Fat accumulation in the liver was significantly reduced by the fiber feeding. WD significantly (p&lt;0.05) diminished the numbers of cecal bacteria within the following taxa: Ruminococcaceae, Bacteroides spp., Coriobacteriaceae ‐ predominant bacteria as compared to the control diet. Ruminococcacace were increased in all the fiber fed mice compared to WD fed. Bacteroides spp. and Coriobacteriaceae increased only in INS, while cellulose feeding reduced Coriobacteriaceae compared to WD. Furthermore, the following genes Hmgcr, Ldlr, Pparα, Fxr and Pxr were significantly upregulated in liver tissues of mice fed INS as compared to WD fed mice.ConclusionINS attenuated the hypercholesterolemic effects of the Western style diet in mice. These effects may be due to bile acid binding activity of INS, changes in the microbiome and increased expression of fatty acid metabolizing genes. Therefore, the insoluble fraction from BSG, consisting of lignin, arabinoxylan and glucan, might be suitable for alleviating the cholesterolemia and thus metabolic diseases.Support or Funding InformationN/AThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Chemical composition, total phenolic content, antioxidant and antinutritional characterisation of exudate gums

Abstract Present study was conducted to determine the chemical composition, antioxidant potential and antinutritional factors in some plant gums that have been commercially used as additives in different food formulations. For this purpose, exudate gums were chosen which included commercially used acacia and karaya gum. The properties were compared with an underutilised gum exuded from apricot tree, called apricot gum. Monosaccharide composition and linkages in gum polysaccharides were studied using Electron spray ionization-mass spectrometry coupled with time-of-flight analyser (ESI-MS-TOF). The assay involves ionization of gum polysaccharides by passing their organic solution into electric field and converting them into a mist of charged droplets that are further analysed and detected using Time-of-flight mass spectrometry. Relative array of peaks elucidated the presence of glucosyl, galactosyl and a small proportion of uronic acid units in all gums. Spectral fragmentation distribution was bimodal with polydispersity values greater than or equal to two (Mw/Mn ≥ 2). In all the antioxidant assays, methanol and ethanol were separately used for extraction. Total phenolic content, ferrous ion chelating ability and reducing power of the gums varied from 0.44 to 1.25 mg GAE/g, 11.80–60.42% and 0.12–0.23 AAE/g, respectively. The DPPH radical scavenging ability (12.03–27.23%) and bile acid binding activity (40.98–46.60%) of the gums was also high. Presence of tannins (0.20–0.73 TAE/g), phytic acid (0.03–0.60 mg/g) and haemagglutinins (0.006–0.11 HU/mg protein) was observed at a very small concentration. On the other hand, the antinutritional factors like terpenoids, saponins and alkaloids were found completely absent.

Extractable and Non-Extractable Polyphenols of Dried Persimmon 'Ichida Gaki' and their &lt;i&gt;in vitro&lt;/i&gt; Bile Acid Binding Activity

Extractable and Non-Extractable Polyphenols of Dried Persimmon 'Ichida Gaki' and their &lt;i&gt;in vitro&lt;/i&gt; Bile Acid Binding Activity

The potential roles of Pinto bean (Phaseolus vulgaris cv. Pinto) bioactive peptides in regulating physiological functions: Protease activating, lipase inhibiting and bile acid binding activities

The aim of the study was to explore the physiological effects of Pinto bean peptides (PBP) and their structure-activity relationships. Five pre-screened PBPs were investigated. The results showed that PBPs enhanced the protease activity ranging from 333 to 400%, and conversely, they inhibited lipase activity ranging from ∼23 to ∼87%. It was suggested that the binding of PBP to pepsin (i.e. Asp52, Tyr113 and Tyr114) resulted in a broad flap extension and enlarged the S3 pocket, which made the active site more accessible, whereas, catalytic residues (i.e. Ser153 and His264) of lipase were found strongly bound to PBPs and preventing the enzyme from hydrolysing lipids. PBPs also shown their ability in binding bile acid ranging from ∼18 to ∼71% via hydrogen bonds to the C5-OH, C11-OH, C15-OH or C21-OH of cholic acid and deoxycholic acid. This study highlighted the effectiveness and mechanism of PBP in preventing obesity, hyperlipidaemia and hypercholesterolemia.

Vegetable Juices and Fibers Reduce Lipid Digestion or Absorption by Inhibiting Pancreatic Lipase, Cholesterol Solubility and Bile Acid Binding

ABSTRACTThe main risk of cardiovascular diseases comes from incorrect eating habits. Restricting unhealthy diets is hard for most people; an alternative dietary intervention is necessary. Dietary products aiding in lipid digestion and absorption inhibitory activities may be beneficial for human health. The potential of juices from 20 vegetables as they affected lipid digestion/absorption processes was investigated. Sweet basil (Ocimum basilicum L.) juice was the most effective pancreatic lipase inhibitor, IC50 1.5 mg·mL−1, and wildbetal leafbush (Piper sarmentosum Roxb.) juice effectively inhibited cholesterol esterase (94% inhibition at 2 mg·mL−1). Juice of green eggplant (Solanum aculeatissimum Jacq.) and carrot (Daucus carota L.) could effectively inhibit cholesterol solubility in lipid micelles due to reduction of cholesterol dispersed in micelles. Fiber from green cabbage (Brassica oleracea L. var. capitata) exhibited bile acid binding activity as high as that of cholestyramine, a bile acid sequestrant. Climbing wattle (Acacia pennata (L.) Willd.) juice, and green cabbage fiber exhibited good inhibition on lipid digestion in vitro. Consumption of these vegetables may inhibit intestinal lipid digestion and delay lipid absorption and might be helpful for weight control and/or lowering of serum lipid levels.

Screening bioactive components affecting the capacity of bile acid binding and pancreatic lipase inhibitory activity

The hypothesis of the current study was that bioactive components including vitamins, dietary fibers, and polyphenols may have both the ability to bind to bile acid and inhibit pancreatic lipase activity. s-carotene, vitamin C, and vitamin U as vitamin, cellulose, s-glucan, and pectin as dietary fibers, and apigenin, curcumin, hesperidin, kaempferol, and quercetin as polyphenols were chosen. The bile acid binding capacity was measured via the furfural–H2SO4 test at the concentration of 2.5 mg/mL with cholestyramine resin used as a positive control (binding value of 87.17 %). The pancreatic lipase inhibitory ability of bioactive components was measured by following the hydrolysis of p-nitrophenyl butyrate. Among the compounds tested, pectin, vitamin C, and s-glucan had the highest binding capacity to bile acid, at 82.7, 67.8, and 33 %, respectively. The results suggest a positive correlation between water solubility and the bile acid binding capacity of the tested bioactive components. At 20 mg/mL concentration, the inhibiting values of all components were relatively lower than the positive control (orlistat, 54.4 %), ranging from 1.3 to 7.6 % of the negative control. Among them, curcumin and cellulose are the highest pancreatic lipase inhibitors with inhibiting values of 7.6 and 7.5 %, respectively. These results imply s-glucan, pectin, vitamin C, cellulose, and curcumin as potential components in reducing the uptake of hydrophobic toxic components through inhibiting micelle formation under human digestion fluid.