Big Stem Research Articles

Identification Of Quantitative Characters of Two Pineapple Varieties (Ananas comosus L. Merr) Resulted By Propagation Of Stem Cutting In Okinawa Prefecture

Pineapple (Ananas comosus L. Merr) is one of the significant yields and the fundamental product created in Okinawa since it is appropriate climatic condition for pineapple improvement. seeds. This study intends to decide the quantitative characters of seedlings from stem engendering of pineapple (Ananas comosus L. Merr) stem cutting on two assortments in Okinawa, Japan. The review was led in December 2022 at Yagaji Island Nursery, Okinawa, Japan. A quantitative descriptive approach was used in the research, and random sampling was used to select 15 seedlings from each variety based on their age, starting at 60 HST, 90 HST, and 120 HST, for a total of 90 samples. Each seed test was noticed in view of boundaries set each month, in particular seedlding level, stem width number of leaves and. Research information were dissected utilizing basic measurement examination. The mean of each character was tried utilizing the T-test at the 5% level and the connection between's qualities was broke down utilizing the r-test at the 5% level. The outcomes showed that there were contrasts in quantitative characters of the two assortments, specifically the N67-10 assortment showing taller seedlings, more number of leaves and bigger stem at the three periods of perception, with the exception of 60 days for stem distance across and 120 days for seedling level. With a range of 0.44-0.77, the phenotypic correlation between characters is real.

Androgen receptor signalling confers clonogenic and migratory advantages in urothelial cell carcinoma of the bladder.

Bladder urothelial cell carcinoma (UCC) incidence is about three times higher in men compared with women. There are several indications for the involvement of hormonal factors in the aetiology of UCC. Here, we provide evidence of androgen signalling in UCC progression. Microarray and qPCR analysis revealed that the androgen receptor (AR) mRNA level is upregulated in a subset of UCC cases. In an AR‐positive UCC‐derived cell line model, UM‐UC‐3‐AR, androgen treatment increased clonogenic capacity inducing the formation of big stem cell‐like holoclones, while AR knockdown or treatment with the AR antagonist enzalutamide abrogated this clonogenic advantage. Additionally, blockage of AR signalling reduced the cell migration potential of androgen‐stimulated UM‐UC‐3‐AR cells. These phenotypic changes were accompanied by a rewiring of the transcriptome with almost 300 genes being differentially regulated by androgens, some of which correlated with AR expression in UCC patients in two independent data sets. Our results demonstrate that AR signals in UCC favouring the development of an aggressive phenotype and highlights its potential as a therapeutic target for bladder cancer.

English

Identification of zygotic and nucellar seedlings of Harumanis mango by morphological approach is impossible or hard to be performed. Therefore, this research were to identify zygotic or nucellar seedlings from Harumanis mango polyembryonic seeds through simple sequence repeat (SSR) molecular markers in relation with germination sequence and vigour of seedlings for source of true-to-type cutting material. The results showed that there were 3 seedlings per seed on average. The result from the molecular analysis of 136 seedlings (45 seeds) showed four zygotic seedlings in seeds 1, 15, 29 and 43 representing 8.9% of seeds evaluated. Most of the zygotic seedlings were found towards the end of germination sequence except for seed number 15 and the rest were considered as nucellar seedlings. Based on the fitted logistic regression, the predicted sequence to obtain 90% nucellar seedlings is 5.47. This means that, the germination sequence of less than 6 has 90% chance of getting nucellar seedlings compared to zygotic seedlings. Morphological characters such as number of leaves, stem diameter and leaf area could also be used as references with germination sequence. The results showed that there were significant (p<0.01) relationships between germination sequence and all the growth variables. All growth variables were negatively correlated with germination sequence. This suggested that in order to have 90% chances of getting nucellar seedling (germination sequence below 6), the seedling needs to exhibit several morphology characters; big stem girth, tall plant, high leaf number and large leaf area. Therefore, choosing vigour seedling will increase chances of getting nucellar seedlings, which can be used as cutting source for true-to-type planting material or for breeding purposes.   Key words: Harumanis, molecular, morphology, nucellar, SSR markers, zygotic.

Effect of Spider Plant Accessions on Phenotypic Traits: Implications for Breeders and Farmers in Kenya

Spider plant (Cleome gynandra L.) is an important African leafy vegetable (ALV) that has been used by local African communities as a source of nutrition in their diets for many years. The plant has recently attracted an increasing demand for its highly nutritive and health-promoting bioactive compounds important in combating malnutrition and reducing human degenerative diseases. Despite the great value of the spider plant, its supply and cultivation remain low, a factor attributed to unavailability of superior genotypes. This study carried out at Ruiru Sub-county, Kiambu County in Kenya sought to establish the influence of genotypic variation on spider plant phenotypic diversity for future breeding and conservations. Experimental plots were set up in the field in Ruiru using split-plot design with three replications. Analysis of variance (ANOVA) was used to assess the significance of variables. Results indicated that genotypes MLSF3, UGSF36, UGSF14, and MLSF17 produced the highest number of flowers, large petioles, big stem sizes and large leaf area respectively. In conclusion, the study recommends the adoption of genotypes MLSF17, UGSF14, UGSF36 and MLSF3 by farmers considering their outstanding positive effect on phenotypic traits.

Dendrocalamus asper productivity after beginning thinning

Dendrocalamus asper is one of bamboo species that is highly required for industrial purposes and community. Beside as the construction materials, shoot of D. asper has a good taste. However, D. asper cultivation faces some obstacles because its big stem and clump disturb other species. Planting in agroforestry model and an appropriate D. asper clump management are the efforts to attract people to plant D. asper. Thinning is applied in D. asper management. This is expected to be able to reduce its canopy impact on the under plants and to improve D. asper productivity. Objective of this research is to find out the influence of beginning thinning on D. asper clump productivity. The research used complete random design with blocks with three treatments of thinning, i.e. a. thinning by eliminating first generation (J1), b. thinning by eliminating first and second generation (J2), c. without thinning (control/J3). The bamboo clump used in the research is two years old. 2 years old clumps. The planting spaces were 10 m x 10 m with a total of 100 clump. The research showed that J2 give the best height growth (5.18 m). It is better than control/J3 (4.5 m), and there were no differences on parameter of diameter and numbers of stem in a clump, although J2 treatment showed the highest average number of stem in a clump.

Water residence times in trees of a neotropical dry forest

Water residence times in trees of a Neotropical dry forest increase with tree diameter and decrease with wood density during the rainy season. In tropical dry forests, tree species with different drought coping strategies co-exist; leaf deciduous and stem-succulent trees are prominent among them. The actual role of stem succulence in tree water use is not fully understood. In a premontane dry forest of southern Ecuador, five tree species were studied in the rainy season including one stem-succulent species (Ceiba trichistandra). All species were studied at the same site and some also across a soil moisture gradient. Deuterium tracing suggests species-specific mean residence times between 11 and 22 days. Mean residence times for Ceiba and two other deciduous tree species were about twice as high as those of two evergreen tree species. Differences in soil moisture did not significantly affect tree water residence times. Our and literature data indicate that across species, residence times increase with tree diameter and decrease with wood density. The stem-succulent Ceiba is characterized by a big stem and a low wood density; the observed residence times in the rainy season were situated on the continuum as derived from a larger data set. The succulent stem may, however, play a special role for leaf flushing at the end of the dry season.

Weaning and Field Survival Responses of Propagules to Propagating Structures and Seedling Types in Sheanut Production in Tropical Africa

The Shea is an economic tree found in West and Central Africa with huge industrial uses in the confectionery, pharmaceutical and cosmetic sectors worldwide. Its rapid multiplication has been hampered by its slow growth and long gestation period. Successes in cutting propagation have been achieved (between 60% - 80%), however weaning of the rooted cuttings for establishment has been a major challenge. Two factorial experiments were carried out in a study in 2012. The objective of this study was to determine the effects of propagating structures and seedling types on the weaning and subsequent field establishment of propagules of Shea tree for plantation establishment. A randomized complete block design with three replications was used. Survival of the rooted cuttings in the mist propagator was very high (93.3%) and comparable to that of the seedlings (100%). The rooted cuttings in mist propagator produced the highest number of leaves, 11 times greater than the least number of leaves produced by seedlings in the lath house. Comparing the seedling types, the rooted cuttings produced significantly greater number of leaves, 4.8 times than the normal seedlings. Rooted cuttings in the mist propagator produced the biggest stem girth significantly greater than the seedlings kept in all the structures. The rooted cuttings in the mist propagator produced 4.4 times bigger stem girths than the seedlings in the lath house which had the smallest stem girth. The rooted cuttings in the mist propagator produced the tallest plants, 1.4 times and 1.9 times significantly taller than the seedlings in the propagating pit and lath house, respectively, which produced the shortest plants. There was a significant relationship between field survival of propagules and the month of establishment expressed as Y (percent survival) = -2844 + 0.070 × (month); P 2 = 0.68; n = 90. Rooted cuttings transplanted in a hole depth of 52.0 cm produced the biggest stem girth, 5.6 times bigger than the stem girth of the seedlings transplanted into any of the three hole depths. Rooted cuttings transplanted into a hole depth of 52.0 cm produced the highest number of leaves, three times more than the seedlings in 26 cm hole depth, which produced the least number of leaves. The study concluded that the mist propagator was the most suitable propagating structure for weaning rooted cuttings of sheanut tree since it resulted in higher survival of the rooted cuttings as well as promoted the growth of the rooted cuttings. For field establishment, rooted cuttings transplanted into hole depth of 52 cm resulted in higher survival rates and performed better in terms of growth than the seedlings. Furthermore, the month of establishment was very critical for the rate of survival of the transplanted propagules of Shea tree.

Polonium (210Po), uranium (234U, 238U) isotopes and trace metals in mosses from Sobieszewo Island, northern Poland

The activity of polonium 210Po and uranium 234U, 238U radionuclides, as well as trace metals in mosses, collected from Sobieszewo Island area (northern Poland), were determined using the alpha spectrometry, AAS (atomic absorption spectrometry) and OES-ICP (atomic emission spectrometry with inductively coupled plasma). The concentrations of mercury (directly from the solid sample) were determined by the cold vapor technique of CV AAS. The obtained results revealed that the concentrations of 210Po, 234U, and 238U in the two analyzed kinds of mosses: schrebers big red stem moss (Pleurozium schreberi) and broom moss (Dicranum scoparium) were similar. The higher polonium concentrations were found in broom moss (Dicranum scoparium), but uranium concentrations were relatively low for both species of analyzed mosses. Among the analyzed trace metals the highest concentration in mosses was recorded for iron, while the lowest for nickel, cadmium and mercury. The obtained studies showed that the sources of polonium and uranium isotopes, as well as trace metals in analyzed mosses are air city contaminations transported from Gdańsk and from existing in the vicinity the phosphogypsum waste heap in Wiślinka (near Gdańsk).

Inoculação de fungos micorrízicos arbusculares em porta-enxerto de pessegueiro cv Okinawa

O presente estudo teve por objetivo avaliar a influência da inoculação de fungos micorrízicos arbusculares (FMA) sobre o crescimento vegetativo, conteúdo de macronutrientes e de substâncias de reserva de plantas do porta-enxerto de pessegueiro cv Okinawa. O experimento foi realizado em telado, e o delineamento experimental utilizado foi o de blocos casualizados, com 20 plantas por parcela e quatro repetições. Foram testadas três espécies de FMA (Acaulospora sp., Glomus clarum e Glomus etunicatum) e um tratamento-testemunha, não-inoculado. A altura, o diâmetro, a área foliar, as biomassas fresca e seca, o conteúdo de macronutrientes e de substâncias de reserva foram avaliados aos 360 dias após a semeadura. Todas as plantas inoculadas com FMA apresentaram maior altura e diâmetro, quando comparadas à testemunha, sendo que Acaulospora sp. promoveu as melhores respostas. Glomus clarum e Glomus etunicatum induziram um crescimento intermediário às plantas. Os FMAs proporcionaram aumento na absorção de nitrogênio, fósforo e potássio, associados à maior altura, diâmetro do colo, área foliar, biomassa fresca e seca da parte aérea e seca das raízes, quando comparadas à testemunha. Todas as plantas inoculadas com FMA tiveram altas taxas de colonização, acima de 90%, sendo que Acaulospora sp. colonizou mais intensamente o sistema radicular das plantas.

Reciprocal expression of 17α-hydroxylase-C17,20-lyase and aromatase cytochrome P450 during bovine trophoblast differentiation: a two-cell system drives placental oestrogen synthesis

No definitive information is yet available on the steroidogenic capacity of the two morphologically distinct cell types forming the bovine trophoblast, the uninucleated trophoblast cells (UTCs) and the trophoblast giant cells (TGCs). Hence, in order to localise 17alpha-hydroxylase-C17,20-lyase (P450c17) on a cellular level and to monitor its expression as a function of gestational age, placentomes from pregnant (days 80-284; n = 19), prepartal (days 273-282; 24-36 h prior to the onset of labour; n = 3) and parturient cows (n = 5) were immunostained for P450c17 using an antiserum against the recombinant bovine enzyme. At all stages investigated, P450c17 was exclusively found in the UTCs of chorionic villi (CV), where staining was ubiquitous between days 80 and 160, but was largely restricted to primary CV and the branching sites of secondary CV between days 160 and 240. Thereafter, a distinct ubiquitous staining reoccurred in the UTCs of all CV in late pregnant, prepartal and parturient animals. Using an antiserum against human aromatase cytochrome P450 (P450arom), specific cytoplasmic staining was observed in TGCs. In placentomes from pregnant cows, staining intensity was higher in mature compared with immature TGCs and was more pronounced in the trophoblast covering big stem villi compared with the trophoblast at other sites of the villous tree. In placentomes of a parturient cow, specific staining was only found in mature TGCs that survived the normal, but substantial, prepartal decline in TGC numbers. These results clearly showed that bovine UTCs and TGCs exhibit different steroidogenic capacities, constituting a 'two-cell' organisation for oestrogen synthesis. P450c17 expression appears to be quickly down-regulated and P450arom is up-regulated when UTCs enter the TGC differentiation pathway.