Background: Health care delivery system is one of the most dynamic areas where the professional growth takes place on an everyday basis. In the country's present scenario, where health services is made available to the community in a variety of forms, by way of government health centers, trust hospitals, religious sponsored small and big hospitals, and private and corporate hospitals, the expectations of the patient have increased toward the quality of health services offered. Aim: The aim of the study was to assess the knowledge and attitude of the employees toward the implementation of quality management system with special reference to Six Sigma. Objectives: 1. To assess the current status of the waiting period at various departments of the selected hospitals. 2. To assess the knowledge regarding Six Sigma. 3. To assess the attitude regarding Six Sigma. 4. To compare the knowledge and attitude of private and government health care organization. Methodology: Research approach: Quantitative approach. Research design: Pre-experimental. Sampling technique: Convenience sampling. Sample size: 60 (calculated by power analysis). Sampling criteria. Inclusive criteria. 1. Those who are involved in hospital administrative work. 2. Those who are available at the time of data collection. 3. Those who can understand English. Exclusive criteria. 1. Who have already done Six Sigma citification. Results: The results showed that the maximum time spent by the patient at the outpatient department (OPD) for registration was 16–20 min. According to the reviews, this is the appropriate time taken by any health care organization to do OPD registration. Very few respond to took more than 25 min, i.e., 3%. Maximum patients spent 21–25 min at the laboratory and only 5% spent <20 min. Time taken by the individual in pharmacy indicates that maximum participants, i.e., 37%, took 16–20 min to get the drugs and 23% of participants took more than 25 min. Regarding the knowledge toward Six Sigma, 22% health care workers in private sector have good knowledge score as compared to government organization, i.e., 10%. Seven percent of health care workers have average knowledge score in private organization and 8% of participants have average knowledge score in government health sector. Regarding the attitude toward Six Sigma, private health care organization workers (53%) have a good attitude score as compared to government organization (18%). Conclusion: Six Sigma principles are well suited to the health care sector due to its zero tolerance for mistakes and Six Sigma's potential for reducing medical errors. Lean Six Sigma projects in healthcare have largely focused on direct care delivery, administrative support, and financial administration. The aim has been to improve clinical processes, to identify and eliminate waste from patient pathways, to enable staff to examine their own workplace, and to increase quality, safety, and efficiency. The concept of Six Sigma is purely corporate in nature. There is a lot of difference between the knowledge and attitude regarding Six Sigma among private and government organizations, and this study proves the same..