Chloride Bicarbonate Research Articles

Distal Renal Tubular Acidosis Mutants of the Kidney Anion Exchanger Disrupt Cytosolic pH and Cause Abnormal Autophagy in Renal Epithelial Cells

Background: Distal renal tubular acidosis (dRTA) is a disease caused by impaired renal acid secretion leading to metabolic acidosis and subsequent biochemical derangements which facilitate formation of kidney stones. This can result from a mutation in the kidney anion exchanger 1 (kAE1) protein, a chloride bicarbonate exchanger in the alpha intercalated cells (A-ICs) of the kidney collecting duct. Studies in human dRTA patients and mouse dRTA models have shown an unexplained depletion in A-ICs. dRTA mouse kidney sections also showed an accumulation of autophagy markers in the remaining A-ICs. Objective and Hypothesis: To better understand the link between dRTA kAE1 mutant expression and A-IC depletion, we hypothesised that dRTA mutant expression activates IC loss through abnormal autophagy and apoptosis. Methods: We used mouse inner medullary collecting duct (mIMCD3) cells expressing two dRTA kAE1 mutants (R589H or S525F) for in vitro studies and two dRTA kAE1 knockin mice L919X and R607H, the murine equivalents of human R901X and R589H mutations, respectively for in vivo studies. We assessed intracellular pH using the ratiometric pH-sensitive fluorescent probe BCECF-AM. Using live cell confocal microscopy, we measured autophagy flux with the eGFP-RFP-LC3 construct. Real time measurements of extracellular acidification and oxygen consumption rates were used to determine cellular glycolytic and oxidative ATP production rates. Results: In vitro, kAE1 R589H and S525F dRTA mutants had significantly more alkaline intracellular pH compared to wild type (WT). Both mutants had higher autophagy induction and accumulation of autolysosomes at steady state. Chemically acidifying cytosolic pH of mutants reversed abnormal autophagy in mutant cells. The kAE1 R589H mutant had significantly lower oxidative ATP production rate whereas S525F mutant had significantly lower glycolytic ATP production rate compared to WT. In vivo, dRTA R607H homozygous mutant mice showed reduced abundance of the vacuolar proton ATPase and of mature Cathepsin D compared to WT. Conclusion: These findings confirm that kAE1 mutant expression alters cytosolic pH and induces abnormal autophagy as a result of reduced overall ATP production and defective lysosomal function. Future work will analyse the processing/traffcking of lysosomal cathepsins and mitochondrial structure and function in mutant cells, and assessing collecting duct lysosome numbers in kidney sections by immunohistochemistry. Our work provides a lead explaining the loss of A-ICs observed in dRTA patients. Canadian Institutes of Health Research (CIHR), Graduate Student Engagement Scholarship — University of Alberta. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Sub-acute nephrotoxicity of ethanol fraction of Senna tora on Wistar Albino Rats

Plant materials as sources of medical compounds continue to play a dominant role in the maintenance of human health since antiquity. The present study was designed to ascertain the toxicity of ethanolic extract of Senna tora leaves on Wistar rats. A total of twenty (25) Wistar rats were distributed into 5 group of 5 animals each. Group I, received normal saline, group II received 100 mg/kg, group III received 200 mg/kg, group IV received 400 mg/kg, and group V received 800 mg/kg of Senna tora leaf fraction respectively. The treatments lasted for 28 days of which the animals were sacrificed. Kidney test which includes the electrolyte like Sodium ion (Na+), potassium ion (k+), bicarbonate (HCO3 ˉ) Chloride ion (Cl-), Urea and Creatinine was done. The results revealed significant increase in the level of sodium ion (Na+), potassium ion (k+), and Chloride ion (Cl-) concentrations between and within the groups of the rats administered dose of 200 mg, 400mg and 800 mg when compared with Normal control. However, the result also revealed that there was no significant increase in the concentration of bicarbonate (HCO3 ˉ) and urea. In addition, the result revealed that there is significant (p≤0.05) increase in the level of serum urea and creatinine concentration between and within the groups of administered dose of 100 mg, 400 mg and 800 mg when compared with the normal control group. There is significant (p˃0.05) increase in total cholesterol (T.CHOL), high density lipoprotein (HDL), low density lipoprotein (LDL), and Triacylglycerol (TGs) on the treatment with Senna tora leaf fraction. The present study revealed that Senna tora as a traditional herb for the treatment and management of diseases associated with kidney toxicity is effective.

Brine–Melts and Fluids of the Fe-F-P-(Ba)-(Sr)-REE Central Asian Carbonatite Province (Southern Siberia and Mongolia): The Petrogenetic Aspects

The carbonatite complexes of the Central Asian carbonatite province comprise the Siberian carbonatites of the Western Transbaikalia and the Central Tuva regions, as well as those from the Mushugai-Khudag complex in Southern Mongolia. They are confined to Late Mesozoic rift structures and have endured considerable tectono-magmatic processes caused by intense plume activity, which also accompanied their formation. A systematic study of melt and fluid inclusions revealed that these carbonatites formed as a result of immiscibility processes in silicate–carbonate (salt) melts, as well as fractional crystallization. Alkaline–carbonatite rocks crystallized in the presence of brine–melts with different compositions, i.e., alkaline–fluorine, carbonate, sulfate, phosphate, and chloride. These melts are responsible for mineralization during the orthomagmatic stage and the primary phase of Fe-F-P-(Ba)-(Sr)-REE ore formation at temperature ranges of 850–830 °C, 650–610 °C, and 560–440 °C and pressures between 290 and 350 MPa. At a later stage, the brine–melts evolved into saline hydrothermal fluids, which are considered to be the source of the second stage of F-(Ba)-(Sr)-REE ore mineralization. The saline crystal–fluid inclusions consist mainly of fluorine–sulfate–carbonate–chloride and bicarbonate–chloride compositions, with temperatures of approximately 480–250 °C and pressures below 250 MPa. The shift from melt to fluid in carbonatite complexes could occur more frequently in nature than previously believed and could also apply to other F-REE carbonatite complexes that are linked to rifting and plume activity in mountain-building zones.

Hydrogeochemical conditions of the Mesozoic hydrogeological basin within the Ravninnoe oil field

Studies of the hydrogeochemical features of groundwater in deep horizons of the West Siberian megabasin are important. They are closely related to applied and fundamental issues of oil and gas hydrogeology and allow studying the processes of formation, accumulation and migration of hydrocarbons.The article deals with our analysis of the hydrogeochemical conditions of the Mesozoic hydrogeological basin within the Ravninnoe oil field. The ion-salt composition of groundwater in deep horizons in the territory is variable. Basically groundwater are of the calcium chloride type (according to V. A. Sulin's classification), but magnesium chloride and sodium bicarbonate types of water are locally distributed. The hydrogeological conditions of the field are manifested in the change in the chemical composition of groundwater and in the vertical hydrochemical zonality. The article describes the features of the regional distribution of reservoir temperatures and reservoir pressures of the Aptian-Albian-Cenomanian, Neocomian and Jurassic hydrogeological complexes. The study of the formation factors of the hydrogeochemical field of these deposits was carried out taking into account paleotectonic, paleogeomorphological and paleohydrogeological reconstructions of the conditions that influenced the accumulation of fluids. Faults are widespread in the deposits of the pre-Jurassic complex, as well as angular unconformities between rocks of different ages, which predetermined the migration of hydrocarbons and the formation of their deposits.

Spatial variability and risk assessment of metals in groundwater of district Kamber-Shahdadkot, Sindh, Pakistan

The study examined the groundwater quality of agricultural based area Nasirabad, Warah and Miro Khan talukas of district Kamber-Shahdadkot. Representative 46 samples were collected commonly used for drinking purposes and analyzed for 25 different quality parameters by using standard analytical procedures. The GIS interpolation tools were used to exhibit the spatial variation of groundwater parameters, for drinking as well as irrigation in the study area. The values of EC and TDS varied from 569 to 17790 μS/cm and 364–11385 mg/L respectively. The results of anions varied HCO3− from 140 to 460, Cl− 21 to 4545 and SO42− 28 to 2050 mg/L and results of cations varied from Na+ 20 to 1380, K+ 03 to 143, Ca2+ 32 to 1302 and Mg2+ 15–728 mg/L. Results of heavy metals varied from Pb 0–44 μg/L, Co 3.9 to 63.8, Cu 0 to 3.9 to 63.8, Fe 10.1 to 335, Cr 0 to 134, Ni 0 to 152, Mn 0 to 140 and Cd 0–29.4 μg/L. The fluoride value of 80.44% samples were above the allowable limits for drinking. The water quality index (WQI) results showed that 70% samples were in poor to unfit water categories. The contaminated index showed 47.82% samples with higher Cd values than 3. The chronic daily intake (CDI) and hazards quotient indices (HQ) were also calculated and HQ did not indicate possible hazourds effects on human health. The samples were examined for principal component analysis, Gibb's diagrams, contour diagrams, scatter diagrams, correlation matrix, Piper diagrams and cluster analysis. The Piper plots showed Na+-Cl-, Ca2+-Mg2+-HCO3- and Ca2+-Mg2+-Cl- species and Gibbs diagrams showed rock and evaporation dominancy. The scatter plots were used to know the hydrochemistry of the area like ion exchange and weathering processes. The quality of water was examined for agriculture, based on chloride bicarbonate ratio, sodium percentage, salinity hazard, Kelly's index, chloro alkaline indices I, chloride-sulphate ratio, residual sodium carbonate, sodium adsorption ratio and permeability index. The results showed that 44–80% samples were fit for irrigation purposes depending upon the requirement of agriculture.

A‐Kinase anchoring protein 2 (AKAP2) and Pendrin; cross‐talk between cell signaling and renal physiology

Kidney tubules play a pivotal role in the maintenance of body fluid homeostasis and pH regulation. Accordingly, kidney disorders are known to be strongly associated with hypertension and acid‐base imbalance. In the kidney, the intercalated cells of the nephron are the main site of acid‐base balance: type A are acid‐secreting whereas type B are base‐secreting. The chloride bicarbonate exchanger Pendrin is present in type B intercalated cells and participates actively in regulating blood pressure and NaCl balance. It is still not entirely deciphered how Pendrin activity is regulated; nevertheless, it was previously demonstrated that Pendrin is regulated by c‐AMP/Protein Kinase A (PKA) signaling pathway. Consequently, in this study, our hypothesis is that the A‐Kinase anchoring protein 2 (AKAP2), strongly expressed in the kidney, regulates in time and space the intracellular signal transduction.In order to investigate AKAP2 involvement in Pendrin regulation and trafficking to the apical plasma membrane, we generated an inducible and nephron‐specific Akap2Pax8/LC1 mice knockout model. By confocal microscopy, fluorescent immunostaining on kidney tissue sections showed that AKAP2 is present in the tubules and most importantly colocalizes with Pendrin at the apical plasma membrane of the intercalated cells. To show that Pendrin and AKAP2 are interacting, we used the Proximity Ligation Assay (PLA) and, positively, we could reveal the association between the two proteins. The latter finding was also confirmed in vitro taking advantage of the Opossum Kidney Cell line (OKP) stably expressing Pendrin and co‐transfected with AKAP2, we could co‐immunoprecipitate Pendrin and AKAP2 after crosslinking on live cells. Fluorescent immunostaining in Akap2Pax8/LC1 show that Pendrin tends to be shifted from the apical membrane (seen in control mice) to intracellular compartments.In conclusion, our data suggest that AKAP2 and Pendrin are interacting, and AKAP2 may play a key role in Pendrin trafficking to the apical plasma membrane, hence in regulating its activity.

Hydrogeochemical Assessment of Selected Springs Water in Aqra Area, Duhok Governorate, Northern Iraq

Fifteen spring water samples were collected from the study area, to identify its quality, as well as the prevailing water type in it. samples were analyzed to determine the concentrations of main cations Ca2+, Mg2+, Na+, and K+, and anions HCO3-, SO42-, Cl- and NO3-, as well as investigating the physio-chemical properties represented by Hydrogen Number, Electrical Conductivity, and Total Dissolved Solids. Piper classification demonstrated that all samples are of type normal earth alkaline water with prevailing bicarbonate with a percentage of 79.9% of all the samples, except for SP4, SP9, and SP11, which characterized with normal alkaline water with prevailing bicarbonate and sulfate or chloride with a percentage of 19.9%. The results of the analysis showed a prevalence of calcium ions over the rest of the cations followed by Mg2+, Na+, and K+, and a prevalence of HCO3- ion over the rest of the anions followed by NO3-, Cl-, and SO42-. The water quality index showed that all samples are categorized within the excellent type for drinking, with a range of 3.14-29.94. In addition, several classifications used (Eaton, 1950, Turgeon, 2000 and Don, 1995) demonstrated that the water is of excellent type to use for irrigation with Na%=2.51, Sodium Adsorption Ratio=0.08, and RSC=-1.65. Using Wilcox Diagram for irrigation water; show that all samples are within the domain of the excellent water for irrigation.

The rumen microbiome inhibits methane formation through dietary choline supplementation

Enteric fermentation from ruminants is a primary source of anthropogenic methane emission. This study aims to add another approach for methane mitigation by manipulation of the rumen microbiome. Effects of choline supplementation on methane formation were quantified in vitro using the Rumen Simulation Technique. Supplementing 200 mM of choline chloride or choline bicarbonate reduced methane emissions by 97–100% after 15 days. Associated with the reduction of methane formation, metabolomics analysis revealed high post-treatment concentrations of ethanol, which likely served as a major hydrogen sink. Metagenome sequencing showed that the methanogen community was almost entirely lost, and choline-utilizing bacteria that can produce either lactate, ethanol or formate as hydrogen sinks were enriched. The taxa most strongly associated with methane mitigation were Megasphaera elsdenii and Denitrobacterium detoxificans, both capable of consuming lactate, which is an intermediate product and hydrogen sink. Accordingly, choline metabolism promoted the capability of bacteria to utilize alternative hydrogen sinks leading to a decline of hydrogen as a substrate for methane formation. However, fermentation of fibre and total organic matter could not be fully maintained with choline supplementation, while amino acid deamination and ethanolamine catabolism produced excessive ammonia, which would reduce feed efficiency and adversely affect live animal performance.

Analysis of performance, bone characteristics, and expression of genes involved in the balance of ionic concentrations in broilers subjected to dietary electrolyte balance levels

ABSTRACT 1. Sodium bicarbonate (NaHCO3), potassium carbonate (K2CO3), and ammonium chloride (NH4Cl) are commonly used to correct dietary electrolyte balance (DEB) in birds. However, there are many gaps in the knowledge of their effects when used simultaneously. This study investigated the effect of DEB levels on performance, femur bone characteristics and the expression of genes related to the balance of ionic concentrations in broilers at 21 days of age. 2. Male Cobb broiler chickens (n = 245), aged 1–21 d, were divided into groups based on a completely randomised design with five DEB levels (110 mEq/kg, 175 mEq/kg, 240 mEq/kg, 305 mEq/kg, and 370 mEq/kg). 3. The performance characteristics measured included body weight (BW), body weight gain (BWG), feed intake (FI), feed conversion (FCR) and body weight birds slaughtered (BWS). 4. The bone variables assessed in the femur were weight (WE), relative bone weight (RBWE), length (L), width (WI), maximum load supported (MLS), bone-breaking resistance (BR), and Seedor index (SI). In addition, the expression of CHP1, SLC9A1, and SLC24A3 in the livers, intestines and kidneys of birds was evaluated. 5. The DEB level of 370 mEq/kg, at Na+ content of 0.48%, resulted in the highest averages for L, MLS, and BR of the femur. This DEB level increased the expression of SLC9A1 in the liver and SLC24A3 in the intestine. A 240 mEq/kg DEB level decreased the expression of CHP1 in the liver, while supplementation with 110 mEq/kg increased the expression of SLC24A3 in the kidney. 6. In conclusion, 370 mEq/kg DEB improved FCR and increased the mean bone characteristics of the femur (L, MLS, and BR) and the expression of SLC9A1 and SLC24A3 in the liver and intestine, respectively. These findings should be considered in future assessments of the effects of DEB levels on broilers.

Errors of Commission in Cardiac Arrest Care in the Intensive Care Unit.

Cardiopulmonary arrests (CPAs) are common in the intensive care unit (ICU). However, effects of protocol deviations on CPA outcomes in the ICU are relatively unknown. To establish the frequency of errors of commission (EOCs) during CPAs in the ICU and their relationship with CPA outcomes. Retrospective analysis of data entered into institutional registry with inclusion criteria of age >18 years and non-traumatic cardiac arrest in the ICU. EOCs consist of administration of drugs or procedures performed during a CPA that are not recommended by ACLS guidelines.Primary outcome: relationship of EOCs with likelihood of return of spontaneous circulation (ROSC). Secondary outcomes: relationship of specific EOCs to ROSC and relationship of EOCs and CPA length on ROSC. Among 120 CPAs studied, there was a cumulative ROSC rate of 66%. Cumulatively, EOCs were associated with a decreased likelihood of ROSC (OR: 0.534, 95% CI: 0.387-0.644). Specifically, administration of sodium bicarbonate (OR: 0.233, 95% CI: 0.084-0.644) and calcium chloride (OR: 0.278, 95% CI: 0.098-0.790) were the EOCs that significantly reduced likelihood of attaining ROSC. Each 5-minute increment in CPA duration and/or increase in number of EOCs corresponded to fewer patients sustaining ROSC. EOCs during CPAs in the ICU were common. Among all EOCs studied, sodium bicarbonate and calcium chloride seemed to have the greatest association with decreased likelihood of attaining ROSC. Number of EOCs and CPA duration both seemed to have an inversely proportional relationship with the likelihood of attaining and sustaining ROSC. EOCs represent potentially modifiable human factors during a CPA through resources such as life safety nurses.

Recurrent noncirrhotic hyperammonemia causing acute metabolic encephalopathy in a patient with a continent ileocecal pouch: a case report

IntroductionAcute encephalopathy, while a common presentation in the emergency department, is typically caused by a variety of metabolic, vascular, infectious, structural, or psychiatric etiologies. Among metabolic causes, hyperammonemia is relatively common and typically occurs in the setting of cirrhosis or liver dysfunction. However, noncirrhotic hyperammonemia is a rare occurrence and poses unique challenges for clinicians.Case presentationHere we report a rare case of a 50-year-old Caucasian female with history of bladder cancer status post chemotherapy, radical cystectomy, and ileocecal diversion who presented to the emergency department with severe altered mental status, combativeness, and a 3-day history of decreased urine output. Her laboratory tests were notable for hyperammonemia up to 289 μmol/L, hypokalemia, and hyperchloremic nonanion gap metabolic acidosis; her liver function tests were normal. Urine cultures were positive for Enterococcus faecium. Computed tomography imaging showed an intact ileoceal urinary diversion with chronic ileolithiasis. Upon administration of appropriate antibiotics, lactulose, and potassium citrate, she experienced rapid resolution of her encephalopathy and a significant reduction in hyperammonemia. Her hyperchloremic metabolic acidosis persisted, but her hypokalemia had resolved.ConclusionThis case is an example of one of the unique consequences of urinary diversions. Urothelial tissue is typically impermeable to urinary solutes. However, when bowel segments are used, abnormal absorption of solutes occurs, including exchange of urinary chloride for serum bicarbonate, leading to a persistent hyperchloremic nonanion gap metabolic acidosis. In addition, overproduction of ammonia from urea-producing organisms can lead to abnormal absorption into the blood and subsequent oversaturation of hepatic metabolic capacity with consequent hyperammonemic encephalopathy. Although this is a rare case, prompt identification and treatment of these metabolic abnormalities is critical to prevent severe central nervous system complications such as altered mental status, coma, and even death in patients with urinary diversions.

Geothermal Prospect Review in the Western Part of Salak Volcano, West Java, Indonesia

The Ertankian Geothermal Prospect is located in the southern area of Bogor District, West Java, Indonesia. From 1982 through the end of 1983, Unocal Geothermal of Indonesia, Ltd. (UGI) conducted extensive geological, geochemical, and geophysical surveys that outlined Ertankian as a potential geothermal field on the flanks of the Salak Volcano. This volcano forms the north-eastern part of the Salak-Perbakti-Gagak Volcanic Massif, which consists of Upper Pleistocene to Recent stratovolcanoes, parasitic vents, and phreatomagmatic craters. The exploration survey included, magnetotelluric and gravity surveys, water geochemistry, thermal manifestation gas, ion, and isotope analyses, and geological, photo-geological interpretation, and photogrammetry interpretation. Kawah Ertankian is a vigorous fumarole area with numerous superheated and boiling steam vents, boiling acid sulfate hot springs, and large areas of acid-altered ground. Elemental sulfur is common around many of the vents. Steam from the Ertankian Fumaroles contains 7 mol% H2S and the maximum reservoir temperature obtained from the NAH-CO2 gas geothermometer was 525°F. The Ertankian Hot Springs have acid sulfate character and yield little information about the underlying hydrothermal system. Bicarbonate chloride warm springs occur on the northern flanks of the Salak Volcano at northwest and northeast locations and interpreted as the outflow product of Ertankian Geothermal System. Extensive areas of low resistivity are distributed across most of the Ertankian Geothermal Prospects. Low resistivity zones are observed around the Kawah Ertankian thermal manifestation complex and broaden to the far northwestern warm spring and Gunung Perbakti in the southwest. The shallowest base of the conductor is observed in the area near Kawah Ertankian and probably extends towards the northwest in the Alpha-19 well area. Towards the south and southwest, a significant deepening of base of the clay cap is observed. The observed resistivity trend coincides with and parallels to the NW-SE South and North Ertankian Faults, which may be the outflow pathway of the hydrothermal system. The lower rock conductance values and deepening of the clay cap base toward the southwest indicate a lower possibility of connection to the main Salak geothermal system. Considering the geological, geochemical, and geophysical data, the Ertankian Geothermal Prospect is presumed to be an encouraging area for further studies.

Dietary Chloride Deficiency Syndrome: Pathophysiology, History, and Systematic Literature Review.

Metabolic alkalosis may develop as a consequence of urinary chloride (and sodium) wasting, excessive loss of salt in the sweat, or intestinal chloride wasting, among other causes. There is also a likely underrecognized association between poor salt intake and the mentioned electrolyte and acid–base abnormality. In patients with excessive loss of salt in the sweat or poor salt intake, the maintenance of metabolic alkalosis is crucially modulated by the chloride–bicarbonate exchanger pendrin located on the renal tubular membrane of type B intercalated cells. In the late 1970s, recommendations were made to decrease the salt content of foods as part of an effort to minimize the tendency towards systemic hypertension. Hence, the baby food industry decided to remove added salt from formula milk. Some weeks later, approximately 200 infants (fed exclusively with formula milks with a chloride content of only 2–4 mmol/L), were admitted with failure to thrive, constipation, food refusal, muscular weakness, and delayed psychomotor development. The laboratory work-up disclosed metabolic alkalosis, hypokalemia, hypochloremia, and a reduced urinary chloride excretion. In all cases, both the clinical and the laboratory features remitted in ≤7 days when the infants were fed on formula milk with a normal chloride content. Since 1982, 13 further publications reported additional cases of dietary chloride depletion. It is therefore concluded that the dietary intake of chloride, which was previously considered a “mendicant” ion, plays a crucial role in acid–base and salt balance.

An Electrochemical Simulation and Treatment Study for the Carbonate and Chloride Based Corrosion of Heritage Silver–Copper Alloys

ABSTRACT This study aimed to investigate the corrosion of a group of historic silver–copper alloy coins and their conservation treatment on defined parameters by potentiostatic methods. Basic copper carbonates and silver chloride were identified on the surface of the coins by a combination of scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) and X-ray diffraction (XRD) analyses. The carbonate and chloride-based corrosion mechanisms were synthetically simulated on modern silver–copper alloy samples (Ag72/Cu28) by anodic polarisation measured by linear sweep voltammetry (LSV) in sodium bicarbonate (NaHCO3) and sodium chloride (NaCl) solutions. Cathodic polarisation curves, measured by LSV, were used to identify the working conditions to reduce the corrosion products on the corroded samples and to treat the actual coins. The reduction process was then monitored by chronoamperometry. Following the potentiostatic reduction process, the metallic copper spots on the treated samples were wiped with cotton swabs moistened with a diluted solution of silver nitrate (AgNO3) to obtain a silvery appearance, and the treatment was completed by a gentle rubbing of the redeposited silver with a cotton swab moistened with a calcium carbonate (CaCO3) suspension followed by rinsing and drying. The corrosion products identified on the synthetically corroded samples indicated that the deterioration mechanisms were dominated by selective attack on the copper-rich phases of the (Ag72/Cu28) alloy, which is consistent with an internal galvanic corrosion phenomenon. The potentiostatic methods showed excellent effectiveness in determining treatment parameters and monitoring the reduction process of the corrosion products on the coins, which helped to preserve surface details in the cleaning process and to prevent over-treatment.

Bactericidal activity of a sanitary and hygienic product based on Bacillus subtilis bacteria
 strains

Introduction. A possible alternative to traditional methods of the treatment with chemical disinfectants are effective and safe preparations based on strains of bacteria Bacillus subtilis. The development of effective hygiene products based on Bacillus subtilis bacteria strains for air sanitation, household items, and rooms that do not adversely affect the body in the presence of humans, animals, and birds is an urgent task of sanitation and hygiene. Material and methods. Suspensions of an equal combination of the bacterial strains Bacillus subtilis TNP-3 and Bacillus subtilis TNP-5 were added to a solution of bicarbonate and sodium chloride. The strains were isolated from the permafrost soils of Yakutia, certified and deposited in the All-Russian collection of microorganisms used in animal husbandry and veterinary medicine (VGNKI, Moscow). The bactericidal activity of the «Probiodes 3 + 5» solutions was studied by the method of “coarse calico tests”. As test cultures there was used 2 billion suspension of diurnal cultures of Str. equi H-34 and Sal. abortus equi BN-12. The effectiveness of sanitization was taken into account according to the level of bacterial contamination - the number of mesophilic aerobic and facultative anaerobic microorganisms (KMAFAnM) on the skin of the nipples of the udder. Results. Reliable disinfection of coarse calico tests contaminated with Str. equi H-34, was established with the use of «Probiodez 3 + 5» (5x10 8 CFU / ml) for 6 hours exposure. By increasing the dose of B. subtilis in the solution «Probiodes 3 + 5» to (1.0 × 109 CFU / ml), there was reliable disinfection of coarse calico tests contaminated with Sal. abortus equi BN-12, exposure 6 hours. The drug «Probiodes 3 + 5» helps to reduce the total bacterial contamination and subclinical mastitis, effective for sanitary-hygienic treatment in dairy cattle breeding. Conclusion. The results of the studies allow making a conclusion that the bacterial strains B. subtilis TNP-3 and B. subtilis TNP-5 are promising for the development of effective, safe hygienic and sanitizing agents for widespread use.)

Evaluation of the carbon dioxide behavior in a thermal aquifer located at Central Mexico and its relation to silicate weathering

A hydrogeochemical and isotopic study was conducted in the western portion of the Celaya Valley Aquifer, Mexico, to assess the role of dissolved carbon dioxide in groundwater contamination. The partial pressure of carbon dioxide value is used to evaluate a complex hydrogeochemical environment, and the carbon dioxide behavior is used to identify the natural and anthropogenic processes related to pollution sources in a thermal aquifer located in the western portion of the Celaya Valley Aquifer. Results indicate that during groundwater migration from the recharge zone to the center of the basin, a hydrogeochemical evolution from calcium bicarbonated and calcium–bicarbonate–chloride type to sodium–bicarbonate type occurs. During this process, deep fluids that migrate through the fault system interact with the aquifer; these fluids contain carbon dioxide released from the metamorphism of the carbonated basement, although another source is not discarded as the dissolved inorganic carbon typical of meteoric groundwater produced from atmospheric carbon dioxide. The hydrogeochemical and isotopic evidences of delta carbon thirteen, delta oxygen eighteen and delta deuterium indicate that the dissolved content of carbon dioxide in the aquifer is involved in the silicate weathering process that plays a major role during water type evolution. In some cases, during weathering, potentially toxic elements are released in amounts that represent an environmental risk, among which arsenic and fluoride stand out, mainly in depth wells. Additionally, other isotopic and hydrogeochemical evidence indicates a biological source of carbon dioxide in the shallow aquifer, primarily due to the agricultural activity carried out in the entire study area.

Assessment of Seawater Intrusion into Shallow Coastal Aquifer in Kanyakumari District, Tamilnadu, India

Improved use of groundwater is often causing intrusion of seawater. The intrusion of seawater also takes place in coastal areas. In this analysis, the distribution and the levels of intrusion in the research area (Kanyakumari district, Tamilnadu, India) are determined. The test was done using random samples at a period of 15 days in which 30 different samples were taken in 5 different locations with a certain distance from the seashore, then chemical analysis including pH, electrical conductivity (EC), total dissolved solids (TDS), chloride, sodium, calcium, magnesium, carbonate, and bicarbonate water quality parameters were carried out. Chloride Bicarbonate Ratio was used for the calculation of the penetration rate of the seawater, and subsequently chloride and electric conductivity were defined as type and quality of water characters. The results showed that the shallow aquifer was intruded by seawater at several sample levels, which was included in the normal to high intrusion classification. The sea water intrusion was classified as freshwater to saltwater, the concentrations in chloride ranged from 159 to 6021 mg / L. The ratio of bicarbonate chloride was 0.28 to 21.46, normally above 15.5, indicating that groundwater was disturbed. As a result, in comparison to standards or other seawater intrusion indicators, Rajakamangalam-well 1 (RM-W1) has been affected by seawater intrusion highly.

Red Blood Cell AE1/Band 3 Transports in Dominant Distal Renal Tubular Acidosis Patients

IntroductionAnion exchanger 1 (AE1) (SLC4A1 gene product) is a membrane protein expressed in both kidney and red blood cells (RBCs): it exchanges extracellular bicarbonate (HCO3–) for intracellular chloride (Cl–) and participates in acid−base homeostasis. AE1 mutations in kidney α-intercalated cells can lead to distal renal tubular acidosis (dRTA). In RBC, AE1 (known as band 3) is also implicated in membrane stability: deletions can cause South Asian ovalocytosis (SAO).MethodsWe retrospectively collected clinical and biological data from patients harboring dRTA due to a SLC4A1 mutation and analyzed HCO3– and Cl– transports (by stopped-flow spectrophotometry) and expression (by flow cytometry, fluorescence activated cell sorting, and Coomassie blue staining) in RBCs, as well as RBC membrane stability (ektacytometry).ResultsFifteen patients were included. All experience nephrolithiasis and/or nephrocalcinosis, 2 had SAO and dRTA (dRTA SAO+), 13 dominant dRTA (dRTA SAO−). The latter did not exert specific RBC membrane anomalies. Both HCO3– and Cl– transports were lower in patients with dRTA SAO+ than in those with dRTA SAO− or controls. Using 3 different extracellular probes, we report a decreased expression (by 52%, P < 0.05) in dRTA SAO+ patients by fluorescence activated cell sorting, whereas total amount of protein was not affected.ConclusionBand 3 transport function and expression in RBCs from dRTA SAO− patients is normal. However, in SAO RBCs, impaired conformation of AE1/band 3 corresponds to an impaired function. Thus, the driver of acid−base defect during dominant dRTA is probably an impaired membrane expression.

Hydrochemistry and Quality Assessment of Water in Tannur Dam, Southern Jordan

The study was undertaken to assess the physicochemical and chemical quality of the Tannur dam water in southern Jordan. The water samples were collected in two intervals the first during May 2015 and the second during September 2015. All samples were analyzed for temperature, conductivity, dissolved oxygen, pH, major cations (Ca2+, Mg2+, K+, Na+), and major anions (Cl-, NO3-, HCO3- and SO42-). The hydrogeochemical analyses of thirty-six water samples were used to determine the properties and type of water in the Tannur dam. The ion concentration in the water samples was from dissolution of carbonate rocks and ion exchange processes in clay. The general chemistry of water samples was typical alkaline earth waters with prevailing bicarbonate chloride. The PHREEQC Hydrogeochemical modeling was used to obtain the saturation indices of specific mineral phases, which might be related to interaction with water and aquifer, and to identify the chemical species of the dissolved ions. Calcite and dolomite solubility were assessed in terms of saturation index where they show positive values indication oversaturated SI > 0. The hydrogeochemistry behavior is rather complicated and is affected by anthropogenic and natural sources. The positive correlation values between various parameters indicate that most of ions result from same lithological sources. The abundance of the major ions in water samples is in the following order: HCO3-> Ca2+ > Cl- > NO3- > SO42-) > Na+ > Mg2+ > K+. Water samples of the Tannur dam are generally very hard, high to very high saline and medium alkaline in nature. High total hardness (TH) and total dissolved solids (TDS) in some samples identify the permissible for domestic and irrigation purposes. According to the residual sodium carbonate, SAR and conductivity values, the studied water is suitable for agricultural purposes.

The Specific Features of the Microcomponent Composition of Pore Solutions in the Rocks of the Bazhenov Formation, Western Siberia

The results of experimental studies of the macrocomponent and microcomponent compositions of pore solutions in the bituminous siliceous shales of the Bazhenov Formation (BF) in Western Siberia with total organic carbon (TOC) contents up to 12.63% (“normal rocks”) and the silty sandstones of the “anomalous” section of the BF with TOC contents no higher than 0.63% (“anomalous” rocks) are presented. The studies of water extracts from core samples were performed. It was found that the pore solutions in the rocks of the “normal” BF rocks are sodium sulfate–bicarbonate, whereas the solutions in the “anomalous” rocks are sodium bicarbonate–chloride. The following tendencies were noted in the pore solutions of both “normal” and “anomalous” rocks compared with the oceanic water: (a) increase in Mn, Co, Ni, Cu, Zn, Mo, Sb, Cd, Ba, V, and Cr concentrations to varying degrees; (b) elevated Cs, Pb, and Ti concentrations in pore solutions of “anomalous” rocks only; (c) reduced Li, B, Rb, and Sr concentrations. A direct correlation between Li, B, Ba, Sr, U, Mo, Cd, Sb, and Ni concentrations in the pore water and total organic carbon (TOC) contents in the rocks was established. The concentrations of V, Cs, Sb, Rb, Ti, and Mn in pore waters display a close to inverse correlation with TOC content in rocks. The content of Ba in the pore solutions of the “normal” BF rocks equals or exceeds the Sr content, which is abnormal compared with formation water in most oil fields and with ocean water and is attributable to the specific features of organic-rich mudstone accumulation, considering that the Sr content in the “anomalous” rocks of the BF is two times higher than the Ba content, which is closer to their usual ratios in most natural waters.