Benzo Pyrene Tetrol Research Articles

Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell.

We report here the application of an antibody-based nanoprobe for in situ measurements of a single cell. The nanoprobe employs antibody-based receptors targeted to a fluorescent analyte, benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyrene (BaP) and of the BaP-DNA adduct. Detection of BPT is of great biomedical interest, since this species can serve as a biomarker for monitoring DNA damage due to BaP exposure and for possible precancer diagnosis. The measurements were performed on the rat liver epithelial clone 9 cell line, which was used as the model cell system. Before making measurements, the cells were treated with BPT. Nanoprobes were inserted into individual cells, incubated 5 min to allow antigen-antibody binding, and then removed for fluorescence detection. We determined a concentration of 9.6+/-0.2x10(-11) M for BPT in the individual cells investigated. The results demonstrate the possibility of in situ measurements inside a single cell using an antibody-based nanoprobe.

Development of Nanosensors and Bioprobes

We describe the development and application of nanosensors having bioreceptor probes for bioanalysis. The nanoprobes were fabricated with optical fibers pulled down to tips having distal end sizes of approximately 30–60 nm. The use of two different types of receptors was investigated. Fiberoptic nanoprobes were covalently bound either with bioreceptors, such as antibodies, or with other receptors, such as cyclodextrins that are selective for the size and chemical structure of the analyte molecules. Theoretical calculations were performed to model the binding of beta-cyclodextrin with pyrene and 5,6-benzoquinoline, and to illustrate the possibility of comparing experimental data with theoretical data. The antibody-based nanoprobe was used for in situ measurements of benzopyrene tetrol in single cells. The performance of the nanosensor is illustrated by intracellular measurements performed on a rat liver epithelial cell line (Clone 9) used as the model cell system. The usefulness and potential of these nanotechnology-based biosensors in biological research and applications are discussed.

Antibody-Based Submicron Biosensor for Benzo[A]Pyrene DNA Adduct

Abstract Fiberoptic-based sensors have been developed for monitoring human exposure and health effects associated with polycyclic aromatic compounds such as benzo[a]pyrene. These sensors take advantage of the physical properties of optical fibers including their relatively small size, remote sensing capabilities, and their inertness to electrical interferences. the recent development of submicron-sized optical fibers provides an opportunity to further reduce the size of fiberoptic-based sensors for application in submicron environments. This paper describes the development of an antibody based submicron fiber biosensor. the antibody-based biosensor is more selective and sensitive than a nonantibody sensor and has an absolute detection limit of ≈ 300 zeptomoles (10−21 moles) for benzo pyrene tetrol, a product of the benzo[a]pyrene DNA adduct. This submicron-size biosensor could provide a useful tool for measurements of toxic chemicals and related metabolites in sub cellularsize environments.