Health Benefits Research Articles

1,25-Dihydroxyvitamin D3 attenuates platelet aggregation potentiated by SARS-CoV-2 spike protein via inhibiting integrin αIIbβ3 outside-in signaling.

Platelet hyperreactivity contributes to the pathogenesis of COVID-19, which is associated with a hypercoagulability state and thrombosis disorder. It has been demonstrated that Vitamin D deficiency is associated with the severity of COVID-19 infection. Vitamin D supplement is widely used as a dietary supplement due to its safety and health benefits. In this study, we investigated the direct effects and underlying mechanisms of 1,25(OH)2D3 on platelet hyperreactivity induced by SRAS-CoV-2 spike protein via Western blot and platelet functional studies in vitro. Firstly, we found that 1,25(OH)2D3 attenuated platelet aggregation and Src-mediated signaling. We further observed that 1,25(OH)2D3 attenuated spike protein-potentiated platelet aggregation in vitro. Mechanistically, 1,25(OH)2D3 attenuated spike protein upregulated-integrin αIIbβ3 outside-in signaling such as platelet spreading and the phosphorylation of β3, c-Src and Syk. Moreover, using PP2, the Src family kinase inhibitor to abolish spike protein-stimulated platelet aggregation and integrin αIIbβ3 outside-in signaling, the combination of PP2 and 1,25(OH)2D3 did not show additive inhibitory effects on spike protein-potentiated platelet aggregation and the phosphorylation of β3, c-Src and Syk. Thus, our data suggest that 1,25(OH)2D3 attenuates platelet aggregation potentiated by spike protein via downregulating integrin αIIbβ3 outside-in signaling.

Selection of a multi-species starter culture for mustard seed fermentation to enhance polyunsaturated fatty acids and improve gastrointestinal health markers

Selection of a multi-species starter culture for mustard seed fermentation to enhance polyunsaturated fatty acids and improve gastrointestinal health markers

Fractionation and characterisation of sialylated-mucin glycoprotein from edible birds' nest hydrolysates through anion exchange chromatography

Edible bird's nest (EBN) is made up of sialylated-mucin glycoprotein with various health benefits due to its high antioxidative activity. However, as a macromolecule with distinct charged sialic acid and amino acids, fractions with different charges would have varied physicochemical properties and antioxidant activity, which have not been studied. Therefore, this study aimed to fractionate and purify the enzymatic hydrolysed of cleaned EBN (EBNhc) and EBN by-product (EBNhbyp) through anion exchange chromatography (AEC), and determine their molecular weights, physicochemical properties, and antioxidative activities. Overall, 26 fractionates were collected from enzymatic hydrolysate by AEC, which were classified into 5 fractions. It was found that the positively charged fraction of EBNhc (CF1) and EBNhbyp (DF1) showed the significantly highest (p < 0.05) soluble protein contents (22.86 and 18.40 mg/g), total peptide contents (511.13 and 800.47 mg/g) and ferric reducing antioxidant power (17.44 and 6.96 mg/g) among the fractionates. In conclusion, a positively charged fraction (CF1 and DF 1) showed more desired physicochemical properties and antioxidative activities. This research suggests the potential of AEC fractionation as a technology to purify EBN and produce positively charged EBN fractionates with antioxidative potential that could be applied as food components to provide health benefits.

A mixed-method analysis of the contribution of informal sport to public health in Australia.

Informal sport is a growth area of sport participation but there has been limited examination of how informal and unstructured forms of participation may contribute to health outcomes that are important for public health. This article aims to address the current lack of data examining the health outcomes associated with informal sport participation and consider the potential role of informal sport within efforts to promote healthier communities through sport. The article seeks to broaden understanding of how informal sport participation can contribute to health outcomes, particularly with regard to increasing physical activity and enhancing mental health and social connection. The article discusses the findings of an Australian mixed-method study that draws on observation, survey, interview and focus group data to examine the prospective health and social benefits of informal sport participation for adults. The findings demonstrate that informal sport participation can contribute to physical and mental health outcomes and facilitate social connection. Analysis of the observation data enabled an examination of the economic value of informal sport in relation to the health benefits it affords. The study provides valuable evidence of the value of informal sport for enhancing community health and broadens understanding of how sport can be utilized as a health promotion resource. The article concludes by suggesting that through leveraging existing infrastructure and the self-organizing aspects of informal sport, local government and health stakeholders can harness its potential to improve public health outcomes and address health inequities.

Edible superhydrophobic coating derived from triterpenoid maslinic acid for bacterial antifouling and enhanced fresh produce food safety

This study introduces an edible superhydrophobic coating for fresh produce derived from triterpenoid maslinic acid (MA), a natural plant compound renowned for its health benefits including antioxidant, antitumor, and anti-inflammatory properties. The study takes advantage of the amphiphilic nature of MA, forming vesicles in aqueous media. Upon deposition onto the substrate, these vesicles contribute to surface roughness, resulting in a nanotextured morphology with nonpolar outer surface. Static water contact angle of MA coatings was 151.6 ± 0.8°, indicating superhydrophobic behavior exhibiting promising bacterial antifouling characteristics with 98.6%, 96.7% and 97.5% reduction against E. coli, Salmonella and Listeria, respectively, compared to uncoated surfaces (i.e., onion skin). The development of edible, superhydrophobic coatings for application on fresh produce advances current fruit and vegetable wax technologies, mostly used for cosmetic and physical barrier purposes rather than more functional ones. Using assembled MA coatings combining superhydrophobicity and health benefits, emerges as an novel and sustainable solution for advancing both fresh produce safety and human well-being.

Spinach genomes reveal migration history and candidate genes for important crop traits.

Spinach (Spinacia oleracea) is an important leafy crop possessing notable economic value and health benefits. Current genomic resources include reference genomes and genome-wide association studies. However, the worldwide genetic relationships and the migration history of the crop remained uncertain, and genome-wide association studies have produced extensive gene lists related to agronomic traits. Here, we re-analysed the sequenced genomes of 305 cultivated and wild spinach accessions to unveil the phylogeny and history of cultivated spinach and to explore genetic variation in relation to phenotypes. In contrast to previous studies, we employed machine learning methods (based on Extreme Gradient Boosting, XGBoost) to detect variants that are collectively associated with agronomic traits. Variant-based cluster analyses revealed three primary spinach groups in the Middle East, Asiaand Europe/US. Combining admixture analysis and allele-sharing statistics, migration routes of spinach from the Middle East to Europe and Asia are presented. Using XGBoost machine learning models we predict genomic variants influencing bolting time, flowering time, petiole color, and leaf surface texture and propose candidate genes for each trait. This study enhances our understanding of the history and phylogeny of domesticated spinach and provides valuable information on candidate genes for future genetic improvement of the crop.

A computational discovery of hexokinase 2 inhibitors from Newbouldia laevis for Hepatocellular carcinoma (HCC) treatment

Newbouldia laevis, also known as the African Border Tree or Fever Tree, is a deciduous tree native to West Africa. The plant is valued for its medicinal properties and is used in traditional medicine for its antimicrobial and anti-inflammatory effects. N. laevis, is a storage tank of phytochemicals with huge health benefits and performances globally for the treatment and management of numerous disease conditions. Limited research exists on the usage of N. laevis for hepatocellular carcinoma (HCC) treatment. This study aims to explore the inhibitory activities of phytochemicals from N. laevis against the hexokinase 2 protein, a target in hepatocarcinoma (HCC) treatment. This study presents a unique in silico approach that includes ligand binding site prediction, molecular docking, molecular dynamics simulation, and Molecular Mechanics Poisson–Boltzmann Surface Area (MM/PBSA) methods. A total of 35 phytochemicals with available 3D structures were identified through literature mining. The literature review highlighted the significance of hexokinase 2 protein as a target inhibitor in the treatment of hepatocellular carcinoma (HCC). Molecular docking experiment with the all the identified phytochemicals and hexokinase 2 revealed that all the identified phytochemicals had potential inhibitory activities against the target protein. Moreover, chrysarobin, apigenin and ursolic acid were the best inhibitors with lowest binding energy of −8.9 kcal/mol, −8.7 kcal/mol, and −8.5 kcal/mol, respectively. The docking experiment was validated by comparing the binding affinities with known reference drug Cabozantinib-S-malate (−8.3 kcal/mol). Further, molecular dynamics studies of complexes with the best docking scores and reference drug complexes were described in detail here. The results of 100 ns modeling (RMSD, RMSF, Rg and SASA) show extraordinary stability during the establishment of complexes with apigenin and ursolic acid, as well as favorable binding energy, which was determined theoretically by means of the MM/PBSA method, thereby increase the probability of their acting as promising and likely hexokinase 2 inhibitors. Therefore, the study predicted that apigenin and ursolic acid could be used as a potential inhibitor/antagonist for the hexokinase 2 enzyme.

Bioactive peptides released by lactic acid bacteria fermented pistachio beverages

Fruit and vegetable are the raw materials for many popular fermented beverages with potential health benefits. In the present study, a novel pistachio beverage fermented with selected strains of lactic acid bacteria belonging to the species Leuconostoc pseudomesenteroides and Companilactobacillus paralimentarius was produced, and the extent of proteolysis was evaluated. Physico-chemical and microbiological analyses, together with peptidomics and proteomics, were carried out on the beverage after 24 h of fermentation, using both the non-inoculated and chemically acidified beverage as controls. Amino acid sequence of hundreds of peptides mainly released from 2S albumin, 11S and 7S globulin were characterized. The number and frequency of identified peptides were higher in beverage started with Leuconostoc pseudomesenteroides, followed by Companilactobacillus paralimentarius and acidic beverage. These results suggest that, in addition to endogenous proteases active at acidic pH, the proteolytic system of LAB directly participated to peptide degradation to some extent. According to the BIOPEP database, a group of 31 peptides were potentially bioactive, and primarily associated with antioxidative properties, ACE and DPP-IV inhibition. The beverage started with Leuconostoc pseudomesenteroides showed the highest amount and number of bioactive peptides. This study lays the foundations for the design of novel pistachio-fermented beverage with potential health properties.

Projected environmental and public health benefits of extended-interval dosing: an analysis of pembrolizumab use in a US national health system

Projected environmental and public health benefits of extended-interval dosing: an analysis of pembrolizumab use in a US national health system

Preparation and characterization of polyphenol-loaded microcapsules based on zeaxylated protein

Pomegranate seeds are rich in polyphenols, known for their significant health benefits. In this study, we developed microcapsules to encapsulate pomegranate seed polyphenols (PSP) using a combination of zeaxylated protein (Zein) and chitosan (Cs). The optimization process involved a Zein to Cs volume ratio of 1:1, a chitosan concentration of 1.0 mg·mL−1, and a polyphenol concentration of 2.0 mg·mL−1. Scanning electron microscopy confirmed the formation of Zein/Cs/PSP complex coacervates, indicating the successful encapsulation. Fourier-transform infrared spectroscopy provided further evidence of electrostatic attraction between PSP and the Zein/Cs matrix. Differential scanning calorimetry analysis demonstrated the successful incorporation of PSP into the Zein/Cs wall material. Moreover, the encapsulated core material exhibited exceptional storage stability and retained its antioxidant activity. Notably, more than 85% of the encapsulated PSP remained intact after 5 hours of simulated intestinal fluid digestion. This study underscores the potential of pomegranate seeds for comprehensive utilization in food engineering.

Therapeutic potentials of Hibiscus trionum: Antioxidant, anti-lipid peroxidative, hypoglycemic, and hepatoprotective effects in type 1 diabetic rats

Recent advances in diabetes treatment have primarily focused on insulin and hypoglycemic agents; however, there is growing interest in exploring herbal and synthetic alternatives. Numerous studies have highlighted the preventive effectiveness of regular plant consumption in managing chronic conditions, particularly diabetes. Hibiscus, a medicinal plant recognized in various cultures, is known for its diverse health benefits. This study investigated the impact of Hibiscus trionum on glycemic control and assessed its influence on glucose and insulin levels in diabetes-induced rats. The concentrations of antioxidant enzymes, particularly superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), were scrutinized across multiple body tissues (plasma, heart, muscle, liver, and kidney). The malondialdehyde (MDA) concentration, an indicator of lipid peroxidation, was examined in both plasma and tissue samples. Serum total cholesterol (TC), triglyceride (TG), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels were evaluated. Diabetic Group (D) exhibited a significant decrease in body weight, increased fluid and food consumption, elevated blood glucose levels, and increased antioxidant enzyme activity. Moreover, the diabetic group also showed increased levels of MDA, TC, TG, AST, and ALT, along with reduced insulin levels, compared to the control group. A substantial improvement in all parameters impaired by diabetes was observed following the application of Hibiscus trionum (HTT) in the Diabetes+HTT group. The antioxidative stress-reducing, lipid peroxidation-improving, and hepatoprotective potential of Hibiscus trionum in mitigating diabetes-induced oxidative stress is noteworthy. These findings indicate that HTT supplementation has valuable beneficial effects in protecting against the harmful impacts of diabetes.

Discovery of bitter masking compounds from Allspice (Pimenta dioica) using sensory guided isolation

Bitter substances in functional foods and beverages can act as nutraceuticals, offering potential health benefits. However, their unpleasant sensory impact reduces the consumption of these foods. Consequently, the discovery of bitter masking compounds is crucial for enhancing the intake of bioactive compounds in functional foods and beverages. Bitter taste is mediated by TAS2Rs, a sub-family of G-protein-coupled receptors. TAS2R14 is especially pivotal in the perception of bitterness, as it is one of the most broadly tuned bitter receptors. In this study, allspice was extracted and purified to yield five single compounds based on sensory guided fractionation. The structures of each compound were determined based on nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HR-MS). In a sensory evaluation, compound 1 exhibited bitter masking activity against quinine. Molecular docking analysis revealed that compound 1 could act as an antagonist of the TAS2R14 bitter receptor.

Non-nutritive Sweeteners: Weight Loss or Weight Gain?

The consumption of non-nutritive sweeteners (NNS) is on the rise among different populations. In parallel, the debate about their potential health benefits and risks remains inconclusive. Numerous published studies elucidate the impact of NNS on general health, weight control, and the risk of certain diseases. However, no definitive conclusions regarding the effect of chronic NNS use on weight have been reached in humans. This review summarizes current evidence related to the biological role of NNS and their subsequent effects on weight. The mechanisms of action through which NNS impact weight are discussed, including their effect on sweet taste receptors, cognition, metabolic and endocrine functions, intestinal microbiota, and adiposity. Conflicting evidence is hindering the formulation of precise recommendations, but the evidence opposing the use of NNS remains weak, and the ultimate impact on weight largely relies on several other behavioral patterns.

Synthesis of N1-caffeoyl-N10-dihydrocaffeoylspermidine (Scotanamine D)

Abstract: N1-caffeoyl-N10-dihydrocaffeoylspermidine (Scotanamine D), a spermidine alkaloid isolated from various plants, is a medicinally valuable natural product. Recent studies have pointed out several health benefits of this compound. However, its synthetic procedures are still not described in the literature. We report the synthesis of this compound following two different schemes comprising multiple steps with excellent overall yields, which are 57% and 81%, respectively. These two synthetic schemes, which use commercially available and cheaper starting materials, can facilitate the large-scale manufacturing of Scotanamine D.

Preparation of Bajra Healthy Wafers

Abstract: Bajra, also known as pearl millet, is a versatile grain rich in essential nutrients and dietary fiber. The incorporation of bajra into snack production, particularly in the form of wafers, presents a promising avenue for creating healthier snack alternatives. Bajra-based wafers offer a blend of taste, crunchiness, and nutritional benefits, catering to the growing demand for wholesome snacks in today's health-conscious society. This abstract explores the development and nutritional aspects of bajrabased wafers, highlighting their potential as a nutritious snack option. The process involves carefully selecting high-quality bajra grains, milling them into flour, and blending them with complementary ingredients to create a crispy, flavorful snack. These wafers not only provide a delightful eating experience but also offer numerous health benefits due to bajra's inherent nutritional profile. The nutritional analysis reveals that bajra wafers are an excellent source of complex carbohydrates, dietary fiber, protein, and essential minerals such as iron and magnesium. Additionally, they are gluten-free, making them suitable for individuals with gluten sensitivities or celiac disease. The inclusion of bajra in snack form contributes to a balanced diet, aiding in weight management and promoting digestive health. Sensory and microbial analyses were conducted to evaluate the product's attributes and safety. The proximate analysis revealed key nutritional parameters such as moisture content (68.8%), total fats (15.55g/200g), protein (9g/200g), carbohydrates (20.2g/200g), crude fiber (6g/200g), ash content (15.34), calcium (234.5mg/200g), and iron (9.425mg/200g). The microbial load was found to be within acceptable limits. Organoleptic assessment, encompassing attributes like color, flavor, texture, taste, and odor, indicated favorable reception by panelists, garnering a high rating score of 8. A packaging solution was chosen based on considerations of product properties and shelf-life requirements, with the product exhibiting a shelf life of 2 to 3 months from the manufacturing date. In conclusion, the developed product offers a nutrient-rich, fiber- and protein-packed snack alternative, catering to health-conscious consumers.

Development of Dry Fruits Laddoo

Abstract: The aim is to prepare Balls (ladoo) as a nutritional point of view and to provide convenience to the consumer. The ingredient which was used for preparation of balls is Dates (Phoenix dactylifera) which contains Energy: 282 cal, Protein: 2.5 g. Carbohydrates: 75 g, Fat: 0.4 g etc. Vitamin B-6 (PYRIDOXINE) is present. Minerals such as Calcium, iron, Magnesium are present in Dates. The flax seeds are very important in regular diet for humans especially for females. They increase nutrient absorption, help in weight loss, gluten free, rich in antioxidants and omega 3 fatty acids. Owing to these health benefits, dietitians and doctors advise to take flax seeds every day. Balls (ladoo) are good and typically made from various flours and dry fruits by various ways. Balls (ladoo) are usually round, sweet, small or large in size. For preparation of Balls (ladoo) ingredients used are pitted dates,flax seeds, almonds, cashews, Jaggery syrup, oats etc. all these ingredients were finely grounded. The formulation was made by varying levels of ingredients. For preparation of Balls (ladoo) all ingredients were roasted and then grounded into fine powder. The dates were pitted and then grounded in mixer grinder. All these grounded ingredients were made into dough by adding Jaggerysyrup. Three trials have been done T1, T2, and T3 by varying proportion of all ingredients and T3 has been selected. Proximate composition of ladoo ware fat content was found out to be 7.08 ± 0.02%, the protein content is 7.85 +0.09 %, the total carbs are 58.40 ± 0.07 %, the energy in ladoo was 328.72 kcal and ash content is about 2.01 ± 0.11% respectively). It was concluded that the Balls (ladoo) can be store for one month in High density polyethylene pouches at room temperature. These balls are rich source of iron so they satisfy consumer’s needs.

Polyphenol content, antioxidant and anti-inflammatory activities of Georgian wine and green Tea

This comprehensive study explores the health-promoting properties of Georgian green tea and red wine, focusing on their antioxidant and anti-inflammatory activities, as well as polyphenol content. Utilizing the DPPH and FRAP methods, we observed impressive results, with green tea showcasing a remarkable FRAP activity of 61.2 % of ascorbic acid activity set as 100 %, surpassing red wine at 18.2 %. Comparative assessments against ascorbic acid for antioxidant activity and Sodium diclofenac for anti-inflammatory effects further underscored green tea's prowess. Intriguingly, green tea exhibited a substantial anti-inflammatory activity of 20 % of Sodium diclofenac also set as 100 %, outshining red wine at 3.7 %. Polyphenol content, a key contributor to health benefits, revealed green tea's concentration at 7.5 % (w/w), red wine's - 4.4 % g/l. These findings highlight the potential of green tea and red wine as a potent source of bioactive compounds with implications for cellular health, inflammation mitigation, and chronic disease prevention. The results prompt a closer look at dietary choices, advocating for the incorporation of green tea to harness its multifaceted health benefits. As we delve into the molecular richness of these beverages, our findings provide valuable insights into the potential therapeutic avenues offered by these popular and culturally significant drinks.

A Comprehensive Examination of the Nutritional Sufficiency of Vegan Recipes Widely Available in the Market

The popularity of plant-based and vegan diets has experienced significant growth in recent years. While adhering to a vegan diet has been linked to various health benefits, it is important to note that certain essential vitamins and minerals, such as vitamins B12 and D, calcium, and iron, may be insufficient in an exclusively plant-based diet. Prolonged inadequate intake of these nutrients can lead to nutritional deficiencies and potentially increase the risk of adverse health outcomes. To further investigate this matter, we conducted a study in which we analyzed a one-week meal plan consisting of vegan recipes from Forks Over Knives (FOK), an organization that advocates for a low-fat, whole-food, vegan diet as a means to prevent or reverse chronic diseases. Through a detailed examination of the meal plan. Based on the evidence derived from this analysis, it is imperative for vegans and their healthcare providers to be cognizant of the potential nutrient deficiencies and associated health consequences that may arise from following this type of diet.

Evaluating the effectiveness of social media in health communication: assessing the impact and outcomes of health communication campaigns conducted on x (Twitter)

Twitter, a leading health communication channel, has effectively promoted health awareness and continues to do so. It provides health information quickly and easily, serving as a vital source of health information. Health practitioners often use Twitter to disseminate health information, a trend that has become more prevalent than engaging audiences in multi-way conversations and interactions. This reassures us of the platform's effectiveness in reaching a broad audience. Twitter has emerged as a powerful tool for reaching a broader audience, offering platforms like Twitter Space for discussion and engagement with health professionals. This technology has brought a new dimension to healthcare, enlightening the public and promoting health behaviors, benefits, challenges, and preventive measures. This research delved into the effectiveness of Twitter in health communication. The study employed a quantitative approach, using a survey to assess the impact of health-related content on Twitter and evaluate the effectiveness of shared health communication. The anticipated outcomes of this research include a comprehensive understanding of the effectiveness of Twitter on health communication, insights into the effectiveness of health content Twitter users access, and recommendations for improving health outcomes through targeted audience campaigns by providing empirical evidence on the effectiveness of Twitter on health communication and a positive and crucial role play in promoting health awareness. Twitter has been used as a tool for health communication campaigns, and many Twitter users benefit from the health information gained there

Assessment of urban air quality in Abidjan in terms of trace metals (copper, lead, zinc) by arborescent vegetation: Case of the species Hibiscus tiliaceus rubra.

Urban air quality is closely linked to human activities. Policies aimed at reducing or monitoring this form of pollution therefore have a dual benefit for health and the environment. Biomonitoring of air quality is one possible scientific approach. In this study, the woody species Hibiscus tiliaceus rubra was used as a biomonitor of air quality in the city of Abidjan. The leaves of the woody species were used to quantify the bioaccumulation of three trace metals: copper, lead and zinc. The assessment was carried out on five main road sections: "Adjamé-Cocody", " Adjamé-Yopougon", "Cocody-Bingerville", abobo-Anyama” and " Adjamé-Plateau". The results obtained show that the content of these trace metals varies according to the road. High levels of copper and zinc and very low levels of lead were initially found in the leaves of the species. The Adjamé-Cocody axis and the Adjamé-Yopougon axis recorded the highest levels of lead. The Adjamé-Plateau axis remains the least contaminated, with relatively low levels of lead.