The foundation design for offshore wind turbines (OWTs) is critical to ensuring their structural integrity and long-term stability. Large-diameter monopiles are widely used for OWTs owing to their proven effectiveness. The conventional design approach, derived from the oil and gas industry’s p-y model, has limitations when applied to monopiles for OWTs. Consequently, a novel design method based on the PISA joint industry research project was developed, which extends beyond the traditional lateral soil resistance (p-y curves) and incorporates additional soil reaction components from shaft friction, pile base shear, and base moment. Unlike the simplistic rule-based conventional approaches, the proposed method necessitates site-specific 3D finite element (FE) simulations for precise calibration of soil reaction curves. Although successfully applied in European offshore wind farms, the PISA method’s performance in regions with distinct seabed compositions, such as China, remains to be verified. Therefore, this paper presents a comprehensive case study conducted at the Xiangshan wind farm, a representative site characterized by layered soil. The study employed in-situ investigations (cone penetration test, borehole) and laboratory tests (direct simple shear, bender element) to delineate the seabed soil profile and derive essential soil parameters for accurate foundation design. To facilitate the design, an Abaqus-based design tool was developed, which automated the extraction of soil reaction curves from the 3D FE simulations, calibration of PISA springs, and execution of one-dimensional beam-spring analyses employing Timoshenko beam theory. Comparative analyses were performed on the computed responses of piles, encompassing load-deflection curves, pile deflections, and bending moment profiles. The outcomes from the 3D FE simulations, uncalibrated rule-based PISA model, and calibrated PISA model were compared to validate the PISA method for monopile design in layered soil conditions.