Engineered Cementitious Composite (ECC) exhibits superior tensile ductility, shear capacity, and crack-control ability in comparison to traditional concrete and fiber reinforced concrete. These attributes render ECC a suitable candidate for addressing the brittle punching shear behavior commonly observed in reinforced concrete (RC) flat slabs. To verify the feasibility of using ECC for strengthening the punching shear capacity of concrete slabs, an investigation on the punching behavior of plain ECC slabs was conducted to ascertain their crack patterns, failure mode, strength and energy absorption capacity by means of experiments on twenty-seven 400 mm×400 mm square slabs. The plain ECC slabs were simply supported on a bespoke steel framework with eight supports, centrically loaded with a concentrated load. The fiber volume fraction (FVF) and span-depth ratio (SDR) are the two variables to be examined for their effects on the punching behavior of ECC slabs. Test results revealed that the incorporation of fibers significantly improved the load-bearing capacity, ductility and energy absorption capacity, while it altered the failure mechanism from brittle to ductile. The critical punching shear angles of all tested plain ECC slabs ranged from 40° to 50°. The ECC specimens possessing an FVF of 2 % exhibited markedly superior tensile capabilities compared to those with a 1 % FVF, as evidenced by direct tensile tests. Nevertheless, the ECC slabs with an FVF of 2 % did not demonstrate commensurate improvements in load-bearing capacity relative to their counterparts containing 1 % FVF. The span-depth ratio significantly impacted the failure mode of plain ECC slabs, yielding to punching shear failure at an SDR of 1, and to flexural failure at SDRs of 2 and 3. Moreover, an increase in the SDR value led to a reduction in both the initial cracking load and the ultimate load, concurrently increasing the associated deflection. In addition, a preliminary theoretical analysis was conducted to estimate the bearing capacity of plain ECC slabs. Flexural strength was estimated by yield line theory (YLT) and punching shear resistance was estimated by the model based on theory of plasticity in slab-column systems. The experimental and theoretical investigations could provide insights into the punching and flexural behavior of ECC slabs, laying a groundwork for the design and development of flat systems strengthened with ECC.