Numerous studies have confirmed that stimulating the mid-brain motor nuclei can regulate movement forcibly for robo-pigeons, but research on behavior modulation using non-motor nuclei is scarce. In this study, we constructed a spatial preference behavior by stimulating the stratum griseum periventriculare (SGP), a nucleus correlated with fear and escape, for robo-pigeons. The study was carried out in a square-enclosed experimental field, with a designated box serving as the 'safe' area for the robo-pigeons. If the robo-pigeon exits this area, the SGP will be stimulated. After a brief training period, the robo-pigeons will have a clear spatial preference for the box. The result from five pigeons has shown that, after simple training, the animals develop a spatial preference for the box. They can quickly return to the box in any situation when the SGP is stimulated, with a success rate exceeding 80% (89.0 ± 6.5%). Moreover, this behavior is highly stable and remains consistent, unaffected by changes in the location of the box or the interference box. The results prove that using the electrical stimulus could enable animals to accomplish more complex tasks. It may offer a novel approach to regulating pigeon behavior and further advance the study of cyborg animals.