Behavioral Experiments Research Articles

Conscious Initiation to Promote Physical Activity: A Behavioral Experiment and A Randomized Controlled Trial Intervention.

Previous studies have investigated the encouragement of healthy behaviors through both conscious manipulation and unconscious priming. However, direct comparisons between these two approaches are limited, resulting in interventions that may lack precision. This study aimed to evaluate the effects of conscious and unconscious priming on the intention to engage in physical activity, with the goal of identifying and applying the most effective method as a targeted intervention to bridge the gap between intention and actual physical activity. In study 1, 116 participants were screened in a 2 × 2 (conscious vs. unconscious) × (prime vs. control) online experiment to examine the influence of implementation intention manipulation and goal priming on physical activity. Building on these results, study 2 employed a randomized controlled trial with 127 participants to assess the effects of conscious mental simulation interventions on physical activity behavior. Study 1 showed that both conscious manipulation (p = .046) and unconscious priming (p = .004) significantly increased the choice of sports activities, with conscious manipulation being more effective. Study 2 found significant impacts of mixed simulation on activity levels immediately and one-week post-intervention (p = .001), with day-after intervention effects notable in the simulation group. This study explored the causal relationship between priming process physical activity, and found out the promotion effect of conscious mental simulation intervention on physical activity.

MacBehaviour: An R package for behavioural experimentation on large language models

The study of large language models (LLMs) and LLM-powered chatbots has gained significant attention in recent years, with researchers treating LLMs as participants in psychological experiments. To facilitate this research, we developed an R package called “MacBehaviour “ (https://github.com/xufengduan/MacBehaviour), which interacts with over 100 LLMs, including OpenAI's GPT family, the Claude family, Gemini, Llama family, and other open-weight models. The package streamlines the processes of LLM behavioural experimentation by providing a comprehensive set of functions for experiment design, stimuli presentation, model behaviour manipulation, and logging responses and token probabilities. With a few lines of code, researchers can seamlessly set up and conduct psychological experiments, making LLM behaviour studies highly accessible. To validate the utility and effectiveness of “MacBehaviour,“ we conducted three experiments on GPT-3.5 Turbo, Llama-2-7b-chat-hf, and Vicuna-1.5-13b, replicating the sound-gender association in LLMs. The results consistently demonstrated that these LLMs exhibit human-like tendencies to infer gender from novel personal names based on their phonology, as previously shown by Cai et al. (2024). In conclusion, “MacBehaviour” is a user-friendly R package that simplifies and standardises the experimental process for machine behaviour studies, offering a valuable tool for researchers in this field.

Novel automated method to assess group dynamics reveals deficits in behavioral contagion in rats with social deficits

Behavioral copying is a key process in group actions, but it is challenging for individuals with autism spectrum disorder (ASD). We investigated behavioral contagion, or instinctual replication of behaviors, in Krushinky-Molodkina (KM) rats (n = 16), a new potential rodent model for ASD, compared to control Wistar rats (n = 15). A randomly chosen healthy Wistar male (“demonstrator rat”) was introduced to the homecage of experimental rats (“observers”) 10–14 days before the experiments to become a member of the group. For the implementation of the behavioral contagion experiment, we used the IntelliCage system, where rats can live in a group of 5–6 rats and their water visits can be automatically scored. During the experiment, the demonstrator was taken out of IntelliCage for a pre-test water deprivation and then placed back for the behavioral contagion test. As a result, a drinking behavior of the water-deprived demonstrator rat prompted water-seeking and drinking behaviors in the whole group. Unlike the Wistar controls, KM observers showed fewer visits to the drinking bottles, particularly lacking inspection visits (i.e., visits without drinking). The control group, in contrast, exhibited a dynamic, cascade-like visiting of the water corners. The proportion of activated observers in KM rats was significantly lower, as compared to Wistar ones, and they did not mimic other observer rats. KM rats, therefore, displayed an attenuated pattern of behavioral contagion, highlighting social deficits in this strain. This study suggests that measuring group dynamics of behavioral contagion in an automated, non-invasive setup offers valuable insights into social behavior in rodents.

Boredom and curiosity: the hunger and the appetite for information

Boredom and curiosity are common everyday states that drive individuals to seek information. Due to their functional relatedness, it is not trivial to distinguish whether an action, for instance in the context of a behavioral experiment, is driven by boredom or curiosity. Are the two constructs opposite poles of the same cognitive mechanism, or distinct states? How do they interact? Can they co-exist and complement each other? Here, we systematically review similarities and dissimilarities of boredom and curiosity with respect to their subjective experience, functional role, and neurocognitive implementation. We highlight the usefulness of Information Theory for formalizing information-seeking in the context of both states and provide guidelines for their experimental investigation. Our emerging view is that despite their distinction on an experiential level, boredom and curiosity are closely related on a functional level, providing complementary drives on information-seeking: boredom, similar to hunger, arises from a lack of information and drives individuals to avoid contexts with low information yield, whereas curiosity constitutes a mechanism similar to appetite, pulling individuals toward specific sources of information. We discuss predictions arising from this perspective, concluding that boredom and curiosity are independent, but coalesce to optimize behavior in environments providing varying levels of information.

Exposure to nature can promote pro‐environmental behavior: Mediating role of temporal discounting

AbstractOne possible explanation for the lack of pro‐environmental behavior among the public is that the benefits of combating climate change are long‐term and distant. Temporal discounting is a pervasive tendency to prefer small immediate gains to large delayed benefits, which may lead to a lack of motivation to engage in pro‐environmental behavior. However, exposure to nature can reduce this tendency and by extension may promote pro‐environmental behavior. Two behavioral experiments were conducted to investigate whether exposure to natural (vs. urban) scenes would induce lower discounting and increase the tendency toward pro‐environmental behavior. We demonstrated that exposure to natural (vs. urban) scenes was associated with a lower level of discounting and a greater tendency toward pro‐environmental behavior, including energy‐saving use of air conditioning (Experiment 1), willingness to participate in beach cleaning (Experiment 2), and choosing meals with less environmental impact (Experiment 2). Mediation analysis indicated that the discounting tendency mediated the relationship between exposure to natural scenes and pro‐environmental behavior. This study provides the first experimental evidence that temporal discounting accounts for the association between exposure to natural (vs. urban) scenes and the tendency toward pro‐environmental behavior. Our findings support the development of a novel strategy for promoting pro‐environmental behavior.

Role of RNA m6A reader YTHDF2 in neurodegeneration of the aged mice

Objective: To investigate the role of RNA m6A reader YTHDF2 in neurodegeneration of the aged mice. Methods: Eighteen 18-month-old control C57BL/6 mice and 22 Ythdf2 conditional knockout (cKO) mice of the same age (that exhibited significant aging characteristics) were used. Five pairs of mice were used for morphological analysis. Thirteen control mice and 17 cKO mice were used for behavioral experiments. Immunofluorescence analysis was performed to detect the expression of YTHDF2 in the brains of 18-month-old C57BL/6 mice. After establishing the neural progenitor cell-specific knockout mice of Ythdf2, their phenotypes were analyzed through comparison of body weight, brain weight and H&E staining. Subsequently, immunohistochemistry and immunofluorescence analyses were used to detect the expression of various neural cell-specific markers in the aged control mice and Ythdf2 cKO mice. Finally, behavioral tests, including the open field test, new object recognition, and water maze, were used to assess the levels of anxiety, depression, learning and memory abilities. Results: Immunofluorescence staining showed that YTHDF2 was mainly expressed in the neurons. Compared with the age-matched control mice, there was no significant change in the body weight of the Ythdf2 cKO mice, but the brain weight decreased significantly (P<0.05). The immunostaining showed that Ythdf2 cKO mice had fewer neurons, fewer astrocytes with defective morphology, more microglia and activation of microglia (P<0.05). Behavioral tests showed that the aged Ythdf2 cKO mice exhibited impaired movement, learning and memory abilities (P<0.05). Conclusions: YTHDF2 is mainly expressed in the neurons of the aged brain. Conditional knockout of Ythdf2 causes quantitative and structural abnormalities in hippocampal neuronal cells, and impairs motor ability and learning and memory of the aged mice, suggesting that YTHDF2 plays an important role in neurodegeneration of the aged mice.

Intranasal oxytocin alleviates postsurgical pain and comorbid anxiety in mice: Participation of BK(Ca) channels in the hippocampus

The affective dimension in postsurgical pain is still poorly understood. Since neuropeptide oxytocin (OXT) has been implicated in a broad spectrum of pain and negative emotion, we investigated the potential therapeutic effect of intranasal OXT on postsurgical pain and associated anxiety in a mice model of plantar incision. The role of large conductance Ca(2+)-activated K(+) (BK(Ca)) channels was explored by using behavioral pharmacology experiments. We reported that plantar incision in mice induced anxiety-like behaviors and mechanical pain hypersensitivity, with a concurrent decrease of the oxytocin receptor (OTR) in the hippocampus. The immunofluorescence staining showed that the OTR were enriched in pyramidal neurons in CA3 subregion of hippocampus and which were highly co-expressed with the BK(Ca) channels in CA3 subregion. Intranasal OXT significantly ameliorated this postsurgical pain and associated anxiety in a dose-dependent manner, while Intra-CA3 microinjection of OTR antagonist atosiban or the BK(Ca) channel blocker paxilline reduced the effect of OXT in incisional mice. Moreover, intra-CA3 microinjection of BK(Ca) channel opener NS1619 produced a similar effect on postsurgical pain and associated anxiety-like behaviors as those observed following intranasal OXT administration. Conversely, intra-CA3 microinjection of BK(Ca) channel blocker paxilline in normal mice was sufficient to evoke mechanical pain hypersensitivity. Taken together, our data suggested that intranasal OXT administration exerted analgesic and anxiolytic effects in incisional mice by opening BK(Ca) channels in the CA3 subregion of hippocampus.

Basal forebrain-lateral habenula inputs and control of impulsive behavior.

Deficits in impulse control are observed in several neurocognitive disorders, including attention deficit hyperactivity (ADHD), substance use disorders (SUDs), and those following traumatic brain injury (TBI). Understanding brain circuits and mechanisms contributing to impulsive behavior may aid in identifying therapeutic interventions. We previously reported that intact lateral habenula (LHb) function is necessary to limit impulsivity defined by impaired response inhibition in rats. Here, we examine the involvement of a synaptic input to the LHb on response inhibition using cellular, circuit, and behavioral approaches. Retrograde fluorogold tracing identified basal forebrain (BF) inputs to LHb, primarily arising from ventral pallidum and nucleus accumbens shell (VP/NAcs). Glutamic acid decarboxylase and cannabinoid CB1 receptor (CB1R) mRNAs colocalized with fluorogold, suggesting a cannabinoid modulated GABAergic pathway. Optogenetic activation of these axons strongly inhibited LHb neuron action potentials and GABA release was tonically suppressed by an endogenous cannabinoid in vitro. Behavioral experiments showed that response inhibition during signaled reward omission was impaired when VP/NAcs inputs to LHb were optogenetically stimulated, whereas inhibition of this pathway did not alter LHb control of impulsivity. Systemic injection with the psychotropic phytocannabinoid, Δ9-tetrahydrocannabinol (Δ9-THC), also increased impulsivity in male, and not female rats, and this was blocked by LHb CB1R antagonism. However, as optogenetic VP/NAcs pathway inhibition did not alter impulse control, we conclude that the pro-impulsive effects of Δ9-THC likely do not occur via inhibition of this afferent. These results identify an inhibitory LHb afferent that is controlled by CB1Rs that can regulate impulsive behavior.

Sulfasalazine improves neuronal function in mice with ischemic stroke by inhibiting the STING/NF-κB pathway.

Inflammation plays an essential role in the pathological processes of ischemic stroke (IS). Sulfasalazine is used in clinical practice for the treatment of inflammatory diseases. This study investigated the effects of sulfasalazine in mice with IS and its underlying mechanisms. We employed an in vivo mice model of middle cerebral artery occlusion (MCAO)/reperfusion, investigating the impact of sulfasalazine on MCAO mice using tetrazolium chloride (TTC) staining, behavioral experiments, and pathological staining. Utilizing of network pharmacology methodologies, we speculated that the protective effect of sulfasalazine against IS may be related to inflammation. The effects of sulfasalazine on inflammation and polarization in oxygen-glucose deprivation/re-oxygenation (OGD/R)-induced BV2 cells were examined through immunofluorescence and PCR techniques. Additionally, the influence of sulfasalazine on the STING/NF-κB pathway was assessed using Western blot and immunofluorescence techniques. Sulfasalazine significantly reduced the infarct area in MCAO mice, restored cerebral blood flow, and improved motor function in the MCAO mice. Pathological staining results indicated that sulfasalazine can mitigate neuronal damage in the cerebral cortex of MCAO mice. Immunofluorescence and PCR testing showed that sulfasalazine promotes M1 to M2 polarization, and reduces inflammation in OGD/R-induced BV2 cells. Furthermore, the Western blot and immunofluorescence results both confirmed that sulfasalazine can inhibit the activation of the STING/NF-κB pathway. This study elucidated the potential therapeutic role of sulfasalazine in IS through its anti-inflammatory effects and modulation of STING/NF-κB pathway, offering novel insights into treatment strategies for IS.

Electroencephalographic Responses to the Number of Objects in Partially Occluded and Uncovered Scenes.

Perceptual completion is ubiquitous when estimating properties such as the shape, size, or number of objects in partially occluded scenes. Behavioral experiments showed that the number of hidden objects is underestimated in partially occluded scenes compared with an estimation based on the density of visible objects and the amount of occlusion. It is still unknown at which processing level this (under)estimation of the number of hidden objects occurs. We studied this question using a passive viewing task in which observers viewed a game board that was initially partially occluded and later was uncovered to reveal its hidden parts. We simultaneously measured the electroencephalographic responses to the partially occluded board presentation and its uncovering. We hypothesized that if the underestimation is a result of early sensory processing, it would be observed in the activities of P1 and N1, whereas if it is because of higher level processes such as expectancy, it would be reflected in P3 activities. Our data showed that P1 amplitude increased with numerosity in both occluded and uncovered states, indicating a link between P1 and simple stimulus features. The N1 amplitude was highest when both the initially visible and uncovered areas of the board were completely filled with game pieces, suggesting that the N1 component is sensitive to the overall Gestalt. Finally, we observed that P3 activity was reduced when the density of game pieces in the uncovered parts matched the initially visible parts, implying a relationship between the P3 component and expectation mismatch. Overall, our results suggest that inferences about the number of hidden items are reflected in high-level processing.

The influence of enriched environment on learning and memory in the Morris water maze in rats with acute and chronic pro-inflammatory stress

It is known that housing in an enriched environment (EE) prevents the development of anxiety-depressive disorders and cognitive impairment caused by various stresses. In a very limited number of studies on learning and memory in the Morris water maze, pro-inflammatory stress was elicited before the exposure of animals in the EE. In this study, we, for the first time, examined the inverse sequence of interaction between EE and stress; in the first place was the action of the EE, while on the second place – the influence of stress. 40 rats aged 25 to 45 days were placed in the EE and 40 other rats were kept under standard conditions. Pro-inflammatory stress in rats of both groups was induced by injection of a bacterial toxin, lipopolysaccharide LPS, once (acute stress, 350 μg/kg) and repeatedly (chronic, 200 μg/kg) 1 hour before the start of behavioral experiments and during their performance. Control animals were injected with saline at the same volume. Rats of the EE group found the platform faster and swam a shorter distance to it than rats of the standard group. Improvements in behavioral parameters were observed in animals with acute and chronic LPS stress, and in control rats. It should also be noted that the dynamics of learning and the features of the long-term and working memory in rats with acute and chronic LPS were similar, which points to a similar effect of EE on functional changes in the brain activity of rats, regardless of the nature of the subsequent stress. The results obtained indicate the important role of EE in beneficially influencing the behavior of rats when searching for a safe platform.

The function of putative appeasement behaviour for access to food in African painted dogs

Abstract In some group-living species that share food, interindividual disagreement on access to food is mediated by several types of negotiation, ranging from appeasing the current owner to reduce aggression and gain access to the food, to harassment where non-owners assert their presence and apply insistent pressure until owners give up their food. In some species, non-owners employ specific behaviours used in other social contexts during negotiation, which makes it challenging to discern their precise functions. The core prediction of the harassment hypothesis is that intense pressure by beggars makes owners abandon their food and leads to food monopolisation, which is not predicted by the appeasement hypothesis. In the African painted dog (Lycaon pictus), non-owners assume a low posture and produce high-pitched vocalisations — a suite of behaviours considered appeasement in non-feeding contexts. This study examined whether these behaviours, referred to collectively as putative appeasement behaviour (PAB), serve as appeasement in the context of feeding or whether they may also, or only, be used for harassment. To this end, we used behavioural experiments in captive parent–offspring or full sibling pairs. Individuals showed more frequent PAB when offered food that could not be easily shared (one vs. two bones). As aggression also only occurred under that condition, PAB seems to have a function in appeasement. However, we observed a positive effect of PAB on food monopolisation by PAB actors but not on co-feeding. Persistent PAB led to monopolisation, indicating that these behaviours serve as a form of harassment in feeding. We did not observe reciprocity between paired individuals, another possible form of food monopolisation besides appeasement and harassment. In conclusion, PAB in the African painted dog functions as both appeasement and harassment, and this highly tolerant species employs negotiation strategies in feeding.

Logistics digitalization and the third-party logistics blue-collar employees performance: the role of paradoxical leader behavior

PurposeThis study examines the impact of third-party logistics (3PL) supervisors’ paradoxical leader behavior (PLB) on the relationship between logistics digitalization and 3PL blue-collar employee performance. 3PLs often lag in digitalization due to blue-collar employees struggling with learning paradoxes, i.e. the tension between abandoning the known methods in favor of new ones. In such situations, 3PL supervisors play a crucial role in helping their subordinates manage these tensions. By incorporating a paradox theory lens, we propose that 3PL supervisors’ PLB acts as a supportive tool, motivating blue-collar employees to address learning paradoxes, thereby improving their operational performance.Design/methodology/approachWe conduct a scenario-based behavioral experiment in which participants assume the role of a package sorter in a fictional 3PL hub setting. Participants engage in a custom-designed package sorting game that mimics a real-life hub sorting operation.FindingsThe results indicate that digitalization combined with supervisors’ PLB significantly improves blue-collar employee performance, with the most substantial improvement observed in the high digitalization and high PLB condition than all other conditions.Originality/valueOur research examines the impact of digitalization on blue-collar employees’ performance through a paradox theory lens. We demonstrate that 3PLs can maximize digitalization benefits by ensuring supervisors exhibit high PLB. Also, we introduce a package sorting game for researchers to conduct experiments related to digitalization and hub operations.

Pregnancy-related acute kidney injury leads to hypertension, reduced kidney function and cognitive impairment in postpartum rats.

Women with hypertensive disorders of pregnancy such as HELLP (hemolysis, elevated liver enzyme, low platelet) Syndrome are affected by acute kidney injury during pregnancy (PR-AKI) at higher rates than women without hypertension. Both hypertensive disorders of pregnancy and Acute Kidney Injury (AKI) outside the context of pregnancy have been associated with an increased risk of developing Chronic Kidney Disease (CKD) and cognitive impairment. In our current study, we set out to determine if PR-AKI led to the development of CKD and impaired cognition in the postpartum period and if HELLP syndrome exacerbates the impairments. Using timed-pregnant Sprague Dawley rats, on gestational day (GD) 12, mini-osmotic pumps infusing anti-angiogenic factors were surgically placed in the intraperitoneal cavity to induce HELLP. On GD18, AKI was induced via bilateral renal reperfusion ischemia surgery. Mean arterial pressure and birth outcomes were used to assess the global effects of AKI, and liver enzymes were used to assess HELLP. CKD was assessed by measuring glomerular filtration rate (GFR), urinary output, and renal fibrosis. Anxiety-like behaviors, object recognition memory, spatial memory, and avoidance memory were assessed via behavioral experiments. HELLP + AKI rats demonstrated more evidence of renal injury, hypertension, and behavioral deficits compared to normal pregnant animals. In addition, AKI had a negative impact on birth outcomes and maternal survival. HELLP + AKI together led to evidence of persistent hypertension, progressive renal dysfunction, and cognitive impairment, which were exacerbated compared to AKI or HELLP alone. These findings suggest that PR-AKI in the presence of a hypertensive disorder of pregnancy, such as HELLP, leads to the development of CKD, cognitive dysfunction, and hypertension.

Neural integration of egocentric and allocentric visual cues in the gaze system.

A fundamental question in neuroscience is how the brain integrates egocentric (body-centered) and allocentric (landmark-centered) visual cues, but for many years this question was ignored in sensorimotor studies. This changed in recent behavioral experiments, but the underlying physiology of ego / allocentric integration remained largely unstudied. The specific goal of this review is to explain how prefrontal neurons integrate eye-centered and landmark-centred visual codes for optimal gaze behavior. First, we briefly review the whole-brain / behavioral mechanisms for ego / allocentric integration in the human and summarize egocentric coding mechanisms in the primate gaze system. We then focus in more depth on cellular mechanisms for ego / allocentric coding in the frontal and supplementary eye fields. We first explain how prefrontal visual responses integrate eye-centered target and landmark codes to produce a transformation toward landmark-centered coordinates. Next, we describe what happens when a landmark shifts during the delay between seeing and acquiring a remembered target, initially resulting in independently co-existing ego / allocentric memory codes. We then describe how these codes are re-integrated in the motor burst for the gaze shift. Deep network simulations suggest that these properties emerge spontaneously for optimal gaze behavior. Finally, we synthesize these observations and relate them to normal brain function through a simplified conceptual model. Together, these results show that integration of visuospatial features continues well beyond visual cortex and suggests a general cellular mechanism for goal-directed visual behaviour.

Development of flame retardant slow release insecticides paint and testing its efficacy for four years against dengue and malaria vectors

Insecticide based paint formulations have been available since years, however the concept of using such paint products at household level did not get attention due to various reasons. The advancement in insecticidal paint technology has steered toward the development and evaluation of such formulations for use against arthropod vectors. The improved insecticidal paint formulations may contain two or more active agents, hence could display different type of activity against the target vectors. In the present study, optimum concentrations of deltamethrin (1%), chlorpyriphos (0.5%) and pyriproxyfen (0.075%) were used as active ingredients (AIs) to develop flame retardant slow-release insecticides paint (FRSRIP) formulation. The developed formulation was tested for physico-chemical properties, toxicity and efficacy against two important mosquito vectors. The formulation was glossy, smooth, uniform and scratch proof. Furthermore, the formulation was flame retardant and conformed to class-A according to the guidelines. Acute oral, dermal and inhalation toxicity suggested that the formulation is safe for use in human dwellings. The formulation was evaluated against Ae. aegypti and An. stephensi mosquitoes in laboratory upto four years. It was found that KDT50 after 24 months (T24) was 14.8 ± 0.8 min and 17.1 ± 1.0 min, while after 48 month (T48) was 21.3 ± 2.0 min and 22.4 ± 1.4 min in both Ae. aegypti and An. stephensi respectively. KDT50 was found varying during the different time intervals (T6 to T48) in both Ae. aegypti (p = 0.01) and An. stephensi (p = 0.0003). Furthermore the corrected mortality (CM) also found statistically declined during the period of evaluation (T6 to T48) in both the test species (F ≥ 42. 4; p ≤ 0.0001). Ae. aegypti mosquitoes that survived FRSRIP exposure exhibited overall decline in total eggs laid, eggs hatched, pupae formed and adult emerged at different time intervals upto T21. Behavioural experiments showed that both the tested species elicited negative response to the test formulation. The concentrations of all the three active agents were estimated by HPLC after different time intervals, however only deltamethrin (0.24%) was found after T48. The developed formulation was stable, safe and effective against mosquito vectors for a considerably longer time. In the pretext of continuous toll of vector borne diseases and trans-boundary expansion of mosquito vectors into new geographical areas, the idea of using insecticidal paint could be a game changer.

Unmanned Aerial Vehicle-based Autonomous Tracking System for Invasive Flying Insects

The Asian hornet or yellow-legged hornet, Vespa velutina nigrithorax, is a global predator of honeybees (Apis mellifera L.) that has become widespread owing to rapid climate change. Herein, we propose a localization system for tracking radio-tagged hornets and discovering hornet hives by combining unmanned aerial vehicles with a trilateration system. By leveraging the homing instinct of hornets, we systematically structured our experiments as a behavioral experiment, ground-truth experiment, and localization experiment. According to the experimental results, we successfully discovered the hives of two of the five hornets tested. Additionally, a comprehensive analysis of the experimental outcomes provided insights into hornet flight patterns and behaviors. The results of this research demonstrate the efficacy of integrating UAVs with radio telemetry for precision object tracking and ecosystem management, offering a robust tool for mitigating the impacts of invasive species on honeybee populations.

Altered default-mode and frontal-parietal network pattern underlie adaptiveness of emotion regulation flexibility following task-switch training.

Emotion regulation flexibility (ERF) refers to one's ability to respond flexibly in complex environments. Adaptiveness of ERF has been associated with cognitive flexibility, which can be improved by task-switching training. However, the impact of task-switching training on ERF and its underlying neural mechanisms remains unclear. To address this issue, we examined the effects of training on individuals' adaptiveness of ERF by assessing altered brain network patterns. Two groups of participants completed behavioral experiments and resting-state fMRI before and after training. Behavioral results showed higher adaptiveness scores and network analysis observed a higher number of connectivity edges, in the training group compared to the control group. Moreover, we found decreased connectivity strength within the default mode network (DMN) and increased connectivity strength within the frontoparietal network (FPN) in the training group. Furthermore, the task-switch training also led to decreased DMN-FPN interconnectivity, which was significantly correlated to increased adaptiveness of ERF scores. These findings suggest that the adaptiveness of ERF can be supported by altered patterns with the brain network through task-switch training, especially the increased network segregation between the DMN and FPN.

Rules or Forward Guidance versus Discretion in Monetary Policy, Evidence from two Behavioral Experiments

Rules or Forward Guidance versus Discretion in Monetary Policy, Evidence from two Behavioral Experiments

Eurasian spruce bark beetle detects lanierone using a highly expressed specialist odorant receptor, present in several functional sensillum types

BackgroundInsects detect odours using odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs) in the antennae. Ecologically important odours are often detected by selective and abundant OSNs; hence, ORs with high antennal expression. However, little is known about the function of highly expressed ORs in beetles, since few ORs have been functionally characterized. Here, we functionally characterized the most highly expressed OR (ItypOR36) in the bark beetle Ips typographus L. (Coleoptera, Curculionidae, Scolytinae), a major pest of spruce. We hypothesized that this OR would detect a compound important to beetle fitness, such as a pheromone component. We next investigated the antennal distribution of this OR using single sensillum recordings (SSR) and in situ hybridization, followed by field- and laboratory experiments to evaluate the behavioural effects of the discovered ligand.ResultsWe expressed ItypOR36 in HEK293 cells and challenged it with 64 ecologically relevant odours. The OR responded exclusively to the monoterpene-derived ketone lanierone with high sensitivity. Lanierone is used in chemical communication in North American Ips species, but it has never been shown to be produced by I. typographus, nor has it been studied in relation to this species’ sensory physiology. Single sensillum recordings revealed a novel and abundant lanierone-responsive OSN class with the same specific response as ItypOR36. Strikingly, these OSNs were co-localized in sensilla together with seven different previously described OSN classes. Field experiments revealed that low release rates of lanierone inhibited beetle attraction to traps baited with aggregation pheromone, with strongest effects on males. Female beetles were attracted to lanierone in laboratory walking bioassays.ConclusionsOur study highlights the importance of the so-called ‘reverse chemical ecology’ approach to identify novel semiochemicals for ecologically important insect species. Our discovery of the co-localization pattern involving the lanierone OSN class suggests organizational differences in the peripheral olfactory sense between insect orders. Our behavioural experiments show that lanierone elicits different responses in the two sexes, which also depend on whether beetles are walking in the laboratory or flying in the field. Unravelling the source of lanierone in the natural environment of I. typographus is required to understand these context-dependent behaviours.