Beetroot Beta Vulgaris Research Articles

Determination of Anti-hypertensive Potential of Beetroot Powder on Hypertensive Patients

Hypertension is a disease in itself and also a risk factor for many other chronic cardiac conditions which are heart attacks, stroke and other CVDs. Beetroot beta vulgaris is a natural root used as a vegetable, having many health potent benefits and nutraceutical cures, most prominent of them is lowering high blood pressure with the help of dietary nitrate in it.The present study was devised to analyze the beetroot powder hypotensive potential on hypertension. For this purpose characterization (proximate analysis, TPC and TFC) of beetroot powder was conducted as part of the study. And blood tests for sodium and potassium electrolytes were done before and after the study duration. Beetroot was obtained by the washing, peeling, and size-reduction, sun drying and grinding of beetroot. Results of proximate analysis of beetroot powder showed that beetroot powder contains 2.18% of protein, 70.42% of moisture, 8.25% of fiber, 5.06 % of ash, 4.00% of fat and 10.09% of nitrogen free extract. Flavonoid content of beetroot was measured at 478 nm and phenolic content showed absorbance at 67.65 nm. Human efficacy was carried out on 30 hypertensive females comprising of 3 groups each group contained 10 subjects, provided them with capsules of beet root powder for 60 days with different dosages G0 acted as a control group, G1 (250 mg) and G2(500 mg). Their Arterial systolic and diastolic blood pressure were monitored on weekly basis every 7th day for 6 weeks. The data obtained was subjected to statistical analysis for theevaluation of results which showed that 500 mg dose of beetroot powder was highly significant for hypertensive patients.

Protective effect beetroot Beta vulgaris extract against H2O2 Induced Oxidative stress in U87MG glioma cells

The study on neurodegenerative associated disorders is of immense clinical interest. Thus in the present investigation hydrogen peroxide was chosen as a precursor to induce oxidative cell damage in the cell cultures. The pigment extracted from red beetroot (Beta vulgaris) was analyzed by quantification of betalains by HPTLC, which revealed that the extract was made of two pig-ments, red and yellow. The red pigment was quantified which was 635ng/20μl (31.75mg/ml). The total phenolics and tannic in the extracts were reported to be 0.031μg/ml and 19.232mg/ml. 200μM of H2O2 was used for ROS production. MTT assay was performed showed the H2O2 treatment resulted in a decrease of cell viability percentage by 46.75 ± 0.018. While the beetroot extract at 10mg/ml increases the cell viability percentage to 55.58± 0.020 in 30min of pre-treatment, but no significant change was observed in post-treatment. Catalase value was highest at 20mg/ml of beet extract (4229.8864μmol of H2O2 consumed/min/mg protein). LDH value 531.67μM/ml in cell lysate, whereas in the LDH exudate highest value recorded was 646.25μM/ml, Catalase exudate activity were higher in both pre and post-treated cells, values were recorded as 4229.88μmol of H2O2 consumed/min/mg protein and 4048.09μmol of H2O2 consumed/min/mg protein respective-ly. Acetylcholine esterase (AChE) activity in post-treated cells was below the H2O2 treatment. In pre-treated cells, all the cells showed lower AChE specific activity in comparison to H2O2 treated cells. The present findings from our work exhibited the protective ability of the red beetroot (Beta vulgaris) against the oxidative stress induced by hydrogen peroxide.