Beetle Callosobruchus Maculatus Research Articles

Evaluation Of Mung Bean (Vigna Radiata) Varieties For Resistance To Bruchid (Callosobruchus Spp) Infestation Using The “No Choice Test"

Mung beans (Vigna radiata) commonly known as green grams, are an important legume crop cultivated for food and as a source of income. Mung bean seeds are highly susceptible to infestation by bruchids (Callosobruchus spp), which can cause significant post-harvest losses, hence impacting on the global food security. Bruchid infestation control remains a priority, and overreliance on chemical pesticides contributes to environmental concerns due to the potential harm to non-target species such as soil and water quality. Investigating natural resistance mechanisms in mung beans can contribute to sustainable and eco-friendly pest management practices. This study aimed at identifying mung bean varieties resistant to storage bruchids using the 'No Choice' test by determining the seed damage. Twenty-three mung bean varieties, both wild and local, were obtained from KALRO Katumani and evaluated against pulse beetle (Callosobruchus maculatus) under laboratory conditions. Fifty seeds of each test sample were placed in separate petri dishes. Five male and female pairs of 0–24-hour-old adults of the beetle were released into each petri dish, covered to prevent insect escape and allow air circulation. The observations were made after 72 hours on oviposition preference by determining the number of eggs laid and percentage seed damage by counting the seeds with one or more holes from the total. The mung bean variety V100-35226 exhibited the highest resistance to bruchid infestations, with minimal seed damage. Other varieties with slight resistance included AMVU-1612, AMVU-1601, AMVU-1603, and V100-1802. The results highlighted significant differences in resistance levels among the mung bean varieties, indicating that some varieties possess natural resistance mechanisms to bruchid infestation

Effect of Microwave Radiation on the Southern Cowpea Bettle <i>Callosobruchus maculatus</i> (F)

This study investigated the effect of microwave radiation at different power levels of 100, 300, and 600 w at different exposure durations of 60, 80, and 100 sec, against eggs, first-instar larvae, and adults of the southern cowpea beetle Callosobruchus maculatus (F). The results showed significant differences in mortality, and 600 w with exposure time of 100 sec being superior. There was 100% mortality of eggs, first-instar larvae and adult; the incubation period of the eggs increased with exposure. The germination of cowpea grains was not significantly affected.

Study of the Effect of Hexane Extracts and Dry Powder of Ocimum Leaves and Flowers on the Percentage of Mortality of Adult Southern Cowpea Beetle Callosobruchus maculatus

Study of the Effect of Hexane Extracts and Dry Powder of Ocimum Leaves and Flowers on the Percentage of Mortality of Adult Southern Cowpea Beetle Callosobruchus maculatus

Gene redundancy and gene compensation of insulin-like peptides in the oocyte development of bean beetle.

Bean beetle (Callosobruchus maculatus) exhibits clear phenotypic plasticity depending on population density; However, the underlying molecular mechanism remains unknown. Compared to low-density individuals, high-density individuals showed a faster terminal oocyte maturity rate. Four insulin-like peptide (ILP) genes were identified in the bean beetle, which had higher expression levels in the head than in the thorax and abdomen. The population density could regulate the expression levels of CmILP1-3, CmILP2-3, and CmILP1 as well as CmILP3 in the head, thorax, and abdomen, respectively. RNA interference results showed that each CmILP could regulate terminal oocyte maturity rate, indicating that there was functional redundancy among CmILPs. Silencing each CmILP could lead to down-regulation of some other CmILPs, however, CmILP3 was up-regulated in the abdomen after silencing CmILP1 or CmILP2. Compared to single gene silencing, silencing CmILP3 with CmILP1 or CmILP2 at the same time led to more serious retardation in oocyte development, suggesting CmILP3 could be up-regulated to functionally compensate for the down-regulation of CmILP1 and CmILP2. In conclusion, population density-dependent plasticity in terminal oocyte maturity rate of bean beetle was regulated by CmILPs, which exhibited gene redundancy and gene compensation.

Comparative effect of Callosobruchus maculatus (Pest) on different pulses

Experiments were undertaken in room circumstances on variety of pulses to investigate the seed damage and weight loss caused by pulse beetle Callosobruchus maculatus. The pulses selected for the study of comparative effect of Callosobruchus maculatus were chick pea (desi chana) “Cicer arietinum”, golden gram (moong daal) “Vigna radiata”, field pea (matar) “Pisum sativum”, Lentil (Masur) “Lens culinaris” and Kabuli chana (Chola) “Cicer arietinum”. The selected five variety of pulses are infested with pulse beetles and kept the pulse boxes for forty-five days without disturbing the pulses.

No‐choice oviposition preferences predict neighborhood effects among host plants in a model system

Abstract Neighbouring plant species can indirectly affect each other's fitness through insect herbivores. For instance, the number of eggs an insect deposits on a focal plant can be influenced by the presence of a neighbouring plant species because of the herbivore's relative host plant preferences. Yet, the relationship between oviposition preferences (measured in no‐choice tests) and the strength and direction of such indirect effects among neighbouring plants remains poorly understood. We performed a lab experiment using bean beetles (Callosobruchus maculatus) and five species of beans to determine if beetles' no‐choice preferences for a neighbouring bean species correlated with the number of eggs laid on a focal species in two‐species environments (diculture). We predicted that as the preference for the neighbouring beans increased compared to the focal host, beetles would lay fewer eggs on the focal bean host. Across all pair‐wise combinations, our prediction was supported: as the relative no‐choice preference of neighbouring beans increased, the number of eggs laid on focal beans decreased. However, this relationship was relatively weak. We also found that beetles tended to lay more eggs in diculture compared to arenas with one bean species. These results provide empirical evidence linking herbivores' no‐choice preferences to the strength of neighbourhood effects and suggest that even within a relatively controlled environment, neighbourhood effects can be weak. Our results also highlight that unexpected changes to the total number of eggs laid by herbivores in mixed‐plant patches relative to monoculture can complicate predictions.

Studying the effect of the pesticides Tondexir and Emamectin benzoate on some biological aspects of the southern cowpea beetle Callosobruchus maculatus

The study aimed to evaluate the effectiveness of Tondexir and Emamectin benzoate pesticides on the southern cowpea beetle, Callosobruchus maculatus. Experiments were conducted using three different concentrations (1, 1.5, 2) ml/1 liter (distilled water) of Tondexir and Emamectin benzoate on 10 selected insects (5 males, 5 females) placed on 10 grams of treated cowpea seeds. The results showed that Tondexir effectively eliminated C. maculatus, achieving a 100% mortality rate after 5 days at a concentration of 2 mL. In contrast, the biopesticide Emamectin benzoate showed high mortality rates for adult southern cowpea beetles, reaching 100% after one day at the same concentration. This confirms the effectiveness and superiority of Emamectin benzoate compared to the control group, which did not show any fatalities. Different concentrations of pesticides had varying effects on spawning rates, with significant differences observed between the treatment and control groups. These results indicate the effect of the pesticides Tondexir and Emamectin benzoate on the egg-laying process of the Southern Cowpea beetle. The study showed that different concentrations of Tondexir and Emamectin benzoate did not lead to egg hatching, while the control group showed a high hatching rate of about 90-93%. This confirms the ability of these pesticides to combat the southern cowpea beetle. In conclusion, the study highlighted the effectiveness of Emamectin benzoate in combating the Southern Cowpea beetle, as the pesticide Emamectin benzoate showed superior results. It also greatly affected egg productivity and hampered the egg-hatching process. These results indicated the possibility of using tondexir and Emamectin benzoate as effective substances to control Callosobruchus maculaus in Cowpea crops.

Effect of alcoholic phenol and nanocapsules extract from grape seed (Vitis vinifera) on egg hatching and adult death of southern cowpea beetles

Pulses are the second most significant economic crop that have been attacked by many storage pests. One of these pests is the cowpea beetle (Callosobruchus maculatus). This study aims to study the effect of phenol grape seed (Vitis vinifera) extract (1000,1500,2000,2500and 3500 mg/L) on eggs and adults. The results showed that the alcoholic extract of the grape seed (Vitis vinifera) recorded for the egg hatching was (80, 56.67, 53.33, 40.00 and 10.00%) respectively, Results in the use of nanocapsules at concentrations of 100 and 500 mg/L showed the egg hatching ratio was (53.33, 16.67%) respectively, while the results of alcoholic extracts of grape seed (Vitis vinifera) recorded for adult mortality was(20.00, 70.00, 73.33, 76.67and96.67%) respectively after 72 hours of treatment , Results in the use of nanocapsules at concentrations of 100 and 500 mg/L showed the adult mortality rate was (26.67, 93.33%) respectively after 72 hours of treatment , The adult mortality rate increases by increasing the concentrations.

Sensitivity of developmental stages of pulse beetle Callosobruchus maculatus (F.) to gamma rays

Sensitivity studies on effects of gamma radiation on different life stages of Callasobruchus maculatus (F.) werecarried out in a growth chamber at temperature of 30ºC and relative humidity of 70 per cent. Two days old eggs, grubs (fourand seventeen days old) and three days old pupae along with green gram seeds were exposed to gamma radiation in range of0-50 Gy, 0-60 Gy, 0-100 Gy and 0-100 Gy, respectively. The radiation doses like 15 Gy, 40 Gy, 100 Gy and 100 Gy wereeffectively prevented adult formation from treated pre-imaginal stages mentioned above. For adult beetles response wasassessed by selecting five pairs of four days old adult male and females along with 30 g greengram seeds kept as a food andwere exposed to gamma radiation in range of 0-140 Gy. The fecundity of adults decreased with the increase of irradiationdose. There was 54.02, 61.59, 69.07, 87.80, 94.36 and 97.45 per cent reduction in egg laying in adults exposed to 20, 40, 60,80, 100 and 120 Gy, respectively. The adults exposed to a radiation dose of 80 Gy and above were capable of egg laying butsuch eggs were not developed to form adult . Therefore, from this study we concluded that all stages of C. maculatus canbe killed at 120 to 150 Gy expose dosage of gamma radiation.

Isolation and Identification of Fungal Species from the Insect Pest Callosobruchus maculatus (F.)

Cowpea seed beetle Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) is one of the most common and economically important pests of stored cereal products worldwide. Furthermore, these beetles can act as vectors for several fungal post-harvest diseases. The current research aimed to isolate the fungi associated with adults of C. maculatus (F.) (Coleoptera: Bruchidae). The present study aimed to isolate and identify fungi associated with adult insects and evaluate their potential as biocontrol agents against the cowpea beetle, C. maculatus. In this study, we collected C. maculatus adults from the Babylon local market and five regions (Hilah, Mahaweel, Al-Mashrou', Al-Haswa and Al-Musayyab) with no history of insecticide exposure. A potato dextrose agar medium was used to isolate the fungi attached to the surfaces of adult beetles. By Morphological and microscopic examination, Molecular identification and Sequencing analysis, Twenty-seven genera of fungi were isolated and identified from adult C. maculatus, including Aspergillus, Penicillium, Candida spp, white mycelium, Curvularia, Cladosporium, Chaetomium, Stachybotrys, Rhizopus, Drechslera, Scopulariopsis, Paecilomyces, Mucor, Geotrichum candidum, Streptomyces, Sarocladium, Beauveria bassiana, Talaromyces varians, Sporothrix flocculasa, Pseudozyma flocculasa, and Isaria fumosorosea. The molecular weights of the PCR products for the fungi isolates ranged from 650-700 bp, and the amplified ITS1-5.8S-ITS4 rDNA region of indigenous entomopathogenic fungal isolates showed a high sequence similarity (99-100%) with B. bassiana and M. anisopliae deposited in the NCBIGenebank. The phylogenetic tree analysis of the ITS region sequences showed a high degree of similarity between the isolates under study, ranging from 96.5-100. However, there were differences in the lines among clades, indicating genetic variation possibly due to mating or mutations in different environments. Our study suggests that the storage pest, C. maculatus, would be essential in spreading fungal contaminants and consequently increasing mycotoxin contamination in stored Cowpea. Keywords: Callosobruchus maculatus, Isolation, Fungi

Isolation and Identification of Fungal Species from the Insect Pest Callosobruchus maculatus (F.)

Cowpea seed beetle Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) is one of the most common and economically important pests of stored cereal products worldwide. Furthermore, these beetles can act as vectors for several fungal post-harvest diseases. The current research aimed to isolate the fungi associated with adults of C. maculatus (F.) (Coleoptera: Bruchidae); the present study aimed to isolate and identify fungi associated with adult insects and evaluate their potential as biocontrol agents against the cowpea beetle, C. maculatus. In this study, we collected C. maculatus adults from the Babylon local market and five regions (Hilah, Mahaweel, Al-Mashrou’, Al-Haswa and Al-Musayyab) with no history of insecticide exposure. A potato dextrose agar medium was used to isolate the fungi attached to the surfaces of adult beetles. By Morphological and microscopic examination, Molecular identification and Sequencing analysis, Twenty-seven genera of fungi were isolated and identified from adult C. maculatus, including Aspergillus, Penicillium, Candida spp, white mycelium, Curvularia, Cladosporium, Chaetomium, Stachybotrys, Rhizopus, Drechslera, Scopulariopsis, Paecilomyces, Mucor, Geotrichum candidum, Streptomyces, Sarocladium, Beauveria bassiana, Talaromyces varians, Sporothrix flocculasa, Pseudozyma flocculasa, and Isaria fumosorosea. The molecular weights of the PCR products for the fungi isolates ranged from 650-700 bp, and the amplified ITS1-5.8S-ITS4 rDNA region of indigenous entomopathogenic fungal isolates showed a high sequence similarity (99-100%) with B. bassiana and M. anisopliae deposited in the NCBIGenebank. The phylogenetic tree analysis of the ITS region sequences showed a high degree of similarity between the isolates under study, ranging from 96.5-100. However, there were differences in the sequences among clades, indicating genetic variation possibly due to mating or mutations in different environments. Our study suggests that the storage pest, C. maculatus, would be essential in spreading fungal contaminants and consequently increasing mycotoxin contamination in stored cowpeas. Keywords: Callosobruchus maculatus, Isolation, Fungi.

Genetic background and thermal regime influence adaptation to novel environment in the seed beetle, Callosobruchus maculatus.

Climate change is associated with the increase in both the mean and variability of thermal conditions. Therefore, the use of more realistic fluctuating thermal regimes is the most appropriate laboratory method for predicting population responses to thermal heterogeneity. However, the long- and short-term implications of evolving under such conditions are not well understood. Here, we examined differences in key life-history traits among populations of seed beetles (Callosobruchus maculatus) that evolved under either constant control conditions or in an environment with fluctuating daily temperatures. Specifically, individuals from two distinct genetic backgrounds were kept for 19 generations at one of two temperatures, a constant temperature (T = 29 °C) or a fluctuating daily cycle (Tmean = 33 °C, Tmax = 40 °C, and Tmin = 26 °C), and were assayed either in their evolved environment or in the other environment. We found that beetles that evolved in fluctuating environments but were then switched to constant 29 °C conditions had far greater lifetime reproductive success compared with beetles that were kept in their evolved environments. This increase in reproductive success suggests that beetles raised in fluctuating environments may have evolved greater thermal breadth than control condition beetles. In addition, the degree of sexual dimorphism in body size and development varied as a function of genetic background, evolved thermal environment, and current temperature conditions. These results not only highlight the value of incorporating diel fluctuations into climate research but also suggest that populations that experience variability in temperature may be better able to respond to both short- and long-term changes in environmental conditions.

Screening of Wild and Cultivated Vigna Accessions for Resistance to Bruchid (Callosobruchus maculatus F.)

The bruchid beetles (Callosobruchus maculatus) is one of the primary causes for the loss in pulses due to the seed damages during storage. The wild relatives of crop species are essential sources of new genes for crop improvement. A set of 40 wild Vigna accessions and cultivated Vigna species were screened for C. maculatus resistance. Based on the results, the accessions viz., V. radiata var. sublobata, V. trilobata and V. umbellata were found resistant as it recorded less adult emergence, lesser seed damage, low index of susceptibility and less seed weight loss among bruchid resistance traits. These traits are more influential principal components and have a high correlation. The identified accessions can be involved in the resistance breeding program as a donor to introgress resistance against bruchid beetles towards developing superior cultivars. One of the identified key traits, viz.,seed weight loss or seed damage or index of susceptibility, should be given more importance while framing the breeding program for reliable and stable bruchid resistance among the cultivar.

Sexual selection buffers the negative consequences of population fragmentation on adaptive plastic responses to increasing temperatures.

Whether sexual selection facilitates or hampers the ability to plastically respond to novel environments might depend on population structure, via its effects on sexual interactions and associated fitness payoffs. Using experimentally evolved lines of the seed beetle Callosobruchus maculatus, we tested whether individuals evolving under different sexual selection (monogamy vs. polygamy) and population spatial structure (metapopulation vs. undivided populations) treatments differed in their response across developmental thermal conditions (control, hot, or stressful) in a range of fitness and fitness-associated traits. We found that individuals from subdivided populations had lower lifetime reproductive success at hot temperatures, but only in lines evolving under relaxed sexual selection, revealing a complex interaction between sexual selection, population structure, and thermal environmental stress on fitness. We also found an effect of population structure on several traits, including fertility and adult emergence success, under exposure to high thermal conditions. Finally, we found a strong negative effect of hot and stressful temperatures on fitness and associated traits. Our results show that population structure can exacerbate the impact of a warming climate, potentially leading to declines in population viability, but that sexual selection can buffer the negative influence of population subdivision on adaptation to warm temperatures.

Impact of alkaloids extract of Moringa olievera Lam. leaves on the development, fertility and demography of the southern cowpea beetle insect Callosobruchus maculatus (F.) (Coleoptera: Bruchidae)

The Southern Cowpea Beetle Callosobruchus maculatus (F.) is one of the most widespread insect pests of stored legumes, causing a considerable loss during storage, decreasing the net weight of the crops, and resulting in reduced the quality of the crops. This study has been conducted to determine the lifetime, fertility and life table parameters of C. maculatus by using an alkaloids extract from Moringa oleifera leaves at different concentrations 1000, 2000, and 3000 ppm. The result was shown that the lowest survival rate was 49% at a concentration of 1000, 2000 ppm, as compared with the control which was 77%. The lowest reproductive rate (Ro) was 4.76 female/female/generation at the concentration of 1000 ppm, compared with the control which was 10.34 female/ female /generation while the lowest rate of egg hatching was 62% at the concentration of 3000 ppm as compared with the control 88%. Similarly, the sex ratio of male: to female was decreased after the use of the three concentrations, mostly at the concentration of 1000 ppm, in which the ratio was 1.31:0.69 compared to 1.11:0.89 for the control. Overall, the life tables curve shows that the survival rate, the ratio of the females and the productivity was affected in a negative way causing a decline in the whole insect population. It is clear from the above that the alkaloids extract of the leaves of the Moringa plant is effective and has an impact on the insect population, and can be an eco-friendly method of controlling on pest population.

Development of bruchid pest and mungbean yellow mosaic virus disease resistance lines in blackgram [Vigna mungo (L.) Hepper] through marker- assisted selection

Bruchid beetles (Callosobruchus maculatus) and mungbean yellow mosaic virus (MYMV) disease are the two major causes of yield constraints in blackgram. Marker-assisted selection was performed among a population developed from the cross combinations of MDU 1 (susceptible female parent) and the TU 68 (resistant male parent) to select for bruchid and MYMV disease resistance. The selected introgression lines (ILs) were significantly superior for bruchid and MYMV disease resistance traits. The introgression lines namely IL-9-1, IL-12-1, IL-12-2, IL-12-7, IL-12-9 and IL-12-10 recorded higher seed yield during the disease prevalent environment than the female parent MDU 1. But the yield performances were lower than the female parent MDU 1 in the disease-free environment. The reduced performance for yield might be due to the linkage drag among the introgression lines. Hence, the linkage drag can be eliminated by the backcross breeding method. However, the successful selection for dual biotic resistance was achieved among the introgression lines. Further, better recombinants must be isolated to obtain good yielding variety and bruchid and MYMV disease resistance.

Efficacy of ethanolic and aqueous extract of some plants on some biological aspects of cowpea beetle Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae) – A comparative study

The present study included to know about the effectiveness of steep chickpea Cicer arietinum L. seeds with ethanolic and aqueous extracts of the Oleander Nerium oleander, Basil Ocimum basilicum, Chinaberry Melia azedarach, and Natgrass Cyperus rotundus leaves with four concentration (0.5, 1.0, 2.5, 5.0) % on some biological sides of the cowpea beetle Callosobruchus maculatus (Fab.). The present study showed the superiority of leaves extracts of Oleander and Chinaberry as compared with leaves extracts of Basil and Natgrass, as well as the superiority of aqueous extracts as compared with ethanolic extracts of the present study plant's leaves on some biological sides of the cowpea beetle C. maculatus (Fab.). Also, the present study showed that the least number of laid eggs was recorded at a concentration of 1.0% of the ethanolic extract of the chinaberry with several 31.5 eggs, the least percentage of hatched eggs was recorded in the aqueous extract of chinaberry at a concentration of 5.0% with a percentage of 64.8%, the highest larval stage period was recorded at a concentration of 5.0% of the ethanolic extract of the chinaberry with a period of 19.8 days. Total mortality (100%) in the larval stage was achieved with a concentration of 5.0% of the aqueous extracts of the Oleander and Chinaberry. The highest pupal stage period was recorded at 5.0% of the ethanolic extract of the chinaberry at 14.8 days. The highest mortality in the pupal stage was recorded at a concentration of 2.5 % of the aqueous extract of the chinaberry with a percentage of 23.0%. The least number of emerged adults in the first generation and least percentage of productivity were recorded at a concentration of 5.0 % of the aqueous extracts of the Oleander and Chinaberry, with several 0.0 adults and a percentage of 0.0%, respectively.The study also showed that the germination percentage of Chickpea seeds was not significantly affected when it was treated with different concentrations of ethanolic and aqueous extracts of the present study plants' leaves.

Age Specific and Fertility Lifetable of <i>Callosobruchus Maculatus</i>(F.) on Chickpea

Pulse beetle (Callosobruchus maculatus) (F.) is a pernicious pest of different stored grain pulses and decrease the nutritional and economic values of the pulses, which can cause huge losses in terms of quality and quantity. The present study was conducted for determining the rate of multiplication of C. maculatus was conducted at ambient temperature and relative humidity under Palampur conditions on preferred host chickpea, Cicer arietinum. Under conditions of abundant space and food supply, population of C. maculatus increased with 0.03485 (r m ) and 1.035 (λ) per day. The population multiplied 1.276 times in one week and mean time for doubling the population was 19.88 days. True generation time was 63.07 days. Life expectancy of adult was 12.37 days. The results of the study pave ways to create awareness among researchers and farmers about the nature and extent of damage caused by the C. maculatesin chickpea.

Increased male investment in sperm competition results in reduced maintenance of gametes.

Male animals often show higher mutation rates than their female conspecifics. A hypothesis for this male bias is that competition over fertilization of female gametes leads to increased male investment into reproduction at the expense of maintenance and repair, resulting in a trade-off between male success in sperm competition and offspring quality. Here, we provide evidence for this hypothesis by harnessing the power of experimental evolution to study effects of sexual selection on the male germline in the seed beetle Callosobruchus maculatus. We first show that 50 generations of evolution under strong sexual selection, coupled with experimental removal of natural selection, resulted in males that are more successful in sperm competition. We then show that these males produce progeny of lower quality if engaging in sociosexual interactions prior to being challenged to surveil and repair experimentally induced damage in their germline and that the presence of male competitors alone can be enough to elicit this response. We identify 18 candidate genes that showed differential expression in response to the induced germline damage, with several of these previously implicated in processes associated with DNA repair and cellular maintenance. These genes also showed significant expression changes across sociosexual treatments of fathers and predicted the reduction in quality of their offspring, with expression of one gene also being strongly correlated to male sperm competition success. Sex differences in expression of the same 18 genes indicate a substantially higher female investment in germline maintenance. While more work is needed to detail the exact molecular underpinnings of our results, our findings provide rare experimental evidence for a trade-off between male success in sperm competition and germline maintenance. This suggests that sex differences in the relative strengths of sexual and natural selection are causally linked to male mutation bias. The tenet advocated here, that the allocation decisions of an individual can affect plasticity of its germline and the resulting genetic quality of subsequent generations, has several interesting implications for mate choice processes.

Effect of age, mating history and temperature on male reproductive costs in the bean beetle Callosobruchus maculatus

Temperature and humidity determine seed viability, but their effect on seed pest reproduction, such as that of the bean beetle Callosobruchus maculatus, remains understudied. Adult male C. maculatus pass large ejaculates to mates while not feeding. Here, we studied ejaculate production and longevity in C. maculatus males at different temperatures and mating patterns. After development at 25, 27 and 29 °C, eclosing males were weighed and prevented from mating (m0) or allowed to mate once with virgin females three (m3) or five (m5) days after eclosion or to mate twice, three and five days after eclosion (m3+5). We weighed males before and after mating to estimate body mass loss due to ejaculate transfer and depletion of resources over time attributed to routine physiology/evaporation. Warmer conditions increased mortality and development rates and tended to decrease body mass. Males with a large body mass at eclosion survived longer than smaller males. The body mass of males was depleted at daily rates equal to 3–5% of body mass at eclosion. The rates of this loss increased in warmer conditions, suggesting that this process was the main determinant of the thermal pattern of mortality. During a single mating, males produced ejaculates that constituted 2–6% of their body mass at eclosion. Contrary to predictions, ejaculate production did not change with thermal conditions. Body mass losses through ejaculate transfer resulted in increased male mortality, and this effect was two times stronger than the effect of a comparable body mass loss through routine physiology/evaporation. Ejaculate mass decreased with age independent of mating history, suggesting that ejaculate production is restricted by routine physiology/evaporation losses. We conclude that the longevity of male C. maculatus is a function of body mass loss, but the two sources of body mass loss considered here, ejaculates and routine physiology, are not equally costly.