Bean Starch Research Articles

The Physicochemical Properties and Structure of Mung Bean Starch Fermented by Lactobacillus plantarum

Understanding the relationship between gel formation and the hierarchical structure of mung bean starch fermented by Lactobacillus plantarum has potential value for its green modification and quality improvement. The variations in characteristics, including gelation characteristics, starch chain, and the molecular order degree of mung bean starch fermented by different L. plantarum, were compared. The results show that in the gelation process, starch began to disintegrate at 65 °C, indicating a critical temperature for structural changes. Compared with the control group, although the effects of different L. plantarum sources on mung bean starch varied, notable improvements were observed in water absorption across all groups of fermented starch, along with reduced free water-soluble substances and enhanced anti-expansion ability. This led to the easier formation of gels with higher viscosity, primarily attributed to decreased crystallinity, increased short-chain amylopectin tendency, an elevated amylose content, and enhanced short-range order when microorganisms acted on the crystallization zone. In conclusion, although L. plantarum came from different sources, its action mode on mung bean starch was similar, which could enhance the gel structure.

Dual modification of starch: Synergistic effects of ozonation and pulsed electric fields on structural, rheological, and functional attributes

Study evaluated the influence of ozonization (O3) time combined with pulsed electric fields (PEF) on the modification of bean starch structure. O₃ was used at a concentration of 0.045 g/L for 60 min (Oz1) and 120 min (Oz2) both individually and in combination with 30 kV/cm (P30). Carbonyl content was higher than the carboxyl content, especially with prolonged treatment times (Oz2), indicating partial oxidation. Additionally, higher levels of amylose and degrees of polymerization (DP ≥ 37 and DP 25–36) were observed in the oxidized starches, with significant changes only when combined with PEF. The main morphological and structural modifications included the presence of agglomerates, partial gelatinization, reduced crystallinity, and lower IR1047/1022 in the granules treated with PEF + O3. Oxidized starches exhibited higher solubility, resulting in lower values for rheological parameters, with PEF + 2 h of O3 (Oz2P) standing out. It can be used as prebiotics, controlled release agents and a texturizer for gluten-free foods.

Effect of starch categories and mass ratio of TA/starch on the emulsifying performance and stability of emulsions stabilized by tannic acid-starch complexes

This study compounded natural corn starch (CS), mung bean starch (MBS) and potato starch (PS) with tannic acid (TA) to stabilize O/W Pickering emulsion. The effect of TA/starch mass ratio (0–0.25) and three starch categories on particle properties, emulsifying properties, lipid oxidation, freeze-thaw stability, emulsion powder and digestive properties were comprehensibly investigated. In detail, the TA/starch complexes size increased gradually (91.14 nm–200.87 nm) and the hydrophobicity first increased and then decreased (TA/CS > TA/MBS > TA/PS) with increasing TA/starch mass ratio. In addition, the emulsifying ability of TA/starch complexes also increased first and then decreased with increasing mass ratio, especially TA/CS system was the best, which was the same as the hydrophobicity conclusion (θow = 80.46°). Moreover, four starch-based emulsion application characteristics were further evaluated to reveal interface structure. Compared to CS and PS system, TA/MBS emulsion had stronger ability to resist the oil oxidation (TBA = 2.54 μg/mL), destruction of ice crystal (whiter emulsion powder) and digestive enzymes (FFAs = 75.33 %). It mainly attributed to the crosslinking network structure and the highest surface load of TA/MBS complexes. This study would provide new ideas for the design and application of emulsifying properties and emulsion stability.

Uncovering the multiscale structure and physicochemical properties of starch extracted from naturally fermented mung bean liquid by wet milling

In this study, mung bean liquid (MBL) was prepared by wet milling and then naturally fermented for 0, 24, 48, 72, and 96 h. Afterward, the starch extracted from MBL was analyzed. The pH of MBL decreases from 6.54 to 4.91 during fermentation. Moreover, the mung bean starch (MBS) granules changed significantly with pitted surfaces, pores and channels. The particle size, molecular weight (Mw) and long chain amylopectin (B2) content of the MBS decreased, whereas the short chain (A) increased from 20.57% to 22.70% with increasing fermentation time. Natural fermentation had no effect on the crystalline type (A) of the MBS but increased the relative crystallinity from 26.53% to 29.23%. However, the intensity ratios of the 995/1022 cm-1 and 1047/1022 cm-1 FTIR spectra increased with fermentation time, reaching maxima of 0.95 and 0.80 after 48 h, respectively. In addition, Fermented MBS increased the trough viscosity (TV), final viscosity (FV), elastic modulus (G') and viscous modulus (G'') but decreased the peak viscosity (PV) and breakdown (BD). Thermal properties revealed that fermentation increased the gelatinisation enthalpy (ΔH) from 9.49 to 10.26 J/g.

Strength, pliability, and hydrophobicity of mung bean starch straws: Orientation change caused by annealing time.

To achieve starch straws with high strength and large toughness, the effects of annealing time on structural and functional performances of mung bean starch straws were studied. The results revealed that with increasing annealing time from 0to 60min, the ratios of 1047cm-1/1022cm-1 in Fourier transform infrared spectroscopy decreased from 1.37 to 1.20, and the relative crystallinities decreased from 12.09% to 11.01%. The relative crystallinity increased to 13.28% when annealing time increased to 120min. The maximum bending force increased from 10.93 to 104.24 N, and modulus of elasticity enhanced from 0.93 to 62.68 N/mm when annealing time increased from 0 to 120min. Starch straws annealed for 120min had the lowest water absorption (94.61%), while starch straws annealed for 60min had the highest water absorption (127.38%). This outcome not only lay a theoretical foundation for preparing biodegradable starch straws with excellent performance, but also apply for beverages, food container, food packaging films, and so on, strongly promoting starch industrial transformation and development.

Investigating the change mechanism and quantitative analysis of minced pork gel quality with different starches using Raman spectroscopy

Minced pork gel often faces challenges during preparation, such as sticking and splitting, which can result in a loose structure, significantly compromises its quality. This study aimed to improve minced pork gel quality by incorporating different starches: mung bean starch (MBS), potato starch (POS), pea starch (PES), and corn starch (CS), focusing on enhancing water-holding capacity (WHC) and gel strength. Raman spectroscopy was employed to investigate the mechanisms behind gel quality changes and to develop quantitative predictions of WHC and gel strength in response to starch addition. The results showed that 15% starch addition was optimal, with POS performing best. Notably, starch addition promoted α-helix and random coil transformation into β-sheet and β-turn in protein structure. Additionally, the starch-minced pork gel system exhibited enhanced cross-linking and density through hydrophobic interactions, contributing to improved WHC and gel strength. Finally, to quantitatively assess the gel quality change under different starches, support vector machine (SVM), uninformative variable elimination-SVM (UVE-SVM), genetic algorithm-SVM (GA-SVM), and adaptive reweighted sampling-SVM (CARS-SVM) modeling were built. Among these, UVE-SVM model achieved the optimal performance for both WHC (Rp2 = 0.8553, RPD = 2.1567) and gel strength (Rp2 = 0.8508, RPD = 2.1981). Therefore, this study demonstrates that the addition of starch, particularly POS, can enhance minced pork gel quality by improving both WHC and gel strength. Moreover, it establishes Raman spectroscopy coupled with UVE-SVM as a reliable method for non-destructive, and rapid detection of these quality parameters in minced pork gel under different starch conditions.

Curcumin-loaded antioxidant oleogels based on native and modified bean starches

Oleogels show promise in the food industry as potential substitutes for saturated fats, providing a medium for incorporating bioactive compounds. Starch modified with octenyl succinic anhydride (OSA) can serve as a structuring agent for vegetable oils due to its amphiphilic nature. This study aimed to develop oleogels based on native starch (NS) or modified starch (MS) bean, sunflower oil, beeswax, and curcumin. The oleogels were evaluated for gel hardness, oil holding capacity, structural stability, weight loss, X-ray diffraction, peroxide value, and antioxidant activity during 30 days of storage. The modification reduced the amylose content by 36%, viscosity by 32%, breakdown by 78%, and retrogradation by 54% of the starch. The oleogels exhibited structural stability and oil binding capacity above 96% during 30 days. Oleogels with NS and 0.5% curcumin were 20% softer than those without curcumin. Over 30 days, the hardness of the oleogels increased by 21%–48%. The addition of 0.5% curcumin in native and OSA starch oleogels increased their antioxidant activities to 41% and 35%, respectively. The texture and antioxidant properties of the oleogels indicate the feasibility of their application in food products.

Optimisation of dry heat treatment conditions for modification of faba bean (Vicia faba L.) starch

Faba bean is a protein-rich starchy grain that is underutilised in the UK. The starch of faba bean can be modified using environmentally friendly methods like dry heat treatment (DHT) to enhance functional and its physicochemical properties. This study investigated the impact of dry heat temperature and time on the structure, functional and physicochemical properties of faba bean starch (FBS) using a two-factor central composite rotatable design. Factors (DHT temperature:100–150 °C and DHT time:0.5–5 h) with their respective α mid-point values led to 13 experimental runs. Selected pasting and functional properties were measured as response variables. Corn starch was included as a reference and compared with the FBS modified using the optimized conditions. DHT increased peak (approx. 2205–2267 cP), final (approx. 3525–3642 cP) and setback (approx. 1887–1993 cP) viscosities but decreased the amylose content of FBS. Colour, as measured by lightness value, morphology and crystalline type were not altered but the starches showed a loss of order and an increase in crystallinity after DHT. FBS appeared resilient to DHT but showed higher swelling power and pasting properties compared to the corn starch control. The optimum DHT conditions to produce starch with desirable properties are a temperature of 100 °C for 0.1716 h, with a desirability factor of 66 %.

Isolation and physiochemical characterization of Red kidney bean (Phaseolus vulgaris L.) starch

The current study focuses on the isolation and physiochemical characterisation of starch from Phaseolus vulgaris L. The yield of the starch was about 25.8 %. The size of the starch granules ranged between 10 and 25μm. The starch had a higher water binding capacity, swelling power and solubility but had low paste clarity. P. vulgaris starch exhibited high freeze thaw stability. The bulk, tapped and particle densities of the starch were 1.64 g/cm3, 2.06 g/cm3 and 0.66 g/cm3 respectively. The porosity of the starch was about 64.1%. The estimated Carr index and Hausner’s ratio indicated good flowability of the starch powder. X-ray diffraction analysis indicated that the starch was C-type starch. Fourier-transform infrared spectroscopy revealed the functional group corresponding to that of starch. Thermo gravimetric analysis indicated good thermostability.

Study on activation energy and water adsorption behavior of adzuki beans under different soaking and cooking processing

Legumes are consumed in various foods, and people frequently expect desirable characteristics from them, such as low cooking times, which minimizes the time and energy required for consuming legumes. Here, we aimed to investigate the activation energy and water adsorption behavior of different combinations of temperatures and times by soaking and cooking adzuki beans. Soaking at 30 °C produced a high-water adsorption balance rate of 98.99 %. Soaking reduced the gelatinization of adzuki bean starch. Furthermore, scanning electron microscopy revealed that, after crushing and filtering separated cotyledon cells, called “bean paste”, the process of soaking and cooking could significantly impact the process and quality of the bean paste. Alternatively, it could maintain a high yield by decreasing bean damage and tissue cracking during cooking. Hence, the results might be used to minimize the processing time, save energy, and improve the product quality of adzuki beans.

Effect of Oat Beta-Glucan on Physicochemical Properties and Digestibility of Fava Bean Starch.

The current research examined the impact of different concentrations of oat beta-glucan (OG) on the in vitro digestibility of fava bean starch (FS). Our pasting analysis demonstrated that OG effectively decreased the viscosity and regrowth of FS, suppressing its in situ regrowth while enhancing the in vitro pasting temperature. Moreover, OG markedly diminished amylose leaching and minimized the particle size of the pasted starch. Rheological and textural evaluations demonstrated that OG markedly diminished the viscoelasticity of the starch and softened the gel strength of the composite system. Structural analysis revealed that hydrogen bonding is the primary interaction in the FS-OG system, indicating that OG interacts with amylose through hydrogen bonding, thereby delaying starch pasting and enhancing the gelatinization characteristics of FS gels. Notably, the incorporation of OG resulted in a reduction in the levels of rapidly digestible starch (RDS) and slowly digestible starch (SDS) in FS, accompanied by a notable increase in resistant starch (RS) content, from 21.30% to 31.82%. This study offers crucial insights for the application of OG in starch-based functional foods.

Nutritional and Health Promoting Attribute of Kidney Beans (Phaseolus vulgaris L.): A Review

Red kidney bean (Phaseolus vulgaris L.) is nutritious and their consumption is associated with many health benefits that can fight against protein malnutrition. Starch, the most common carbohydrate reserve in plants, is present in legume seeds and has been associated with both cultural and nutritional advantages. Kidney bean seeds' main constituent is starch, which accounts for 25–45% of their dry matter. It also goes by the names Rajmash and common bean. Kidney bean starches are more soluble and have less swelling than cereal starches, which suggests that they have more functional qualities. Due to its properties and interactions with other ingredients, particularly water and lipids, which can greatly affect the final product's quality, starch is of interest to both the food industry and human nutrition as a macro-ingredient in many foods.

Three nonconventional starch: Comparison of physicochemical properties and in vitro digestibility.

Extraction of starch from waste is also an effective way to recover resources and provide new sources of starch. In this study, starch was isolated from white kidney bean residue, chickpea residue, and tiger nut meal after protein or oil extraction, and the morphology of starch particles was observed to determine their physicochemical properties and in vitro digestibility. All these isolated starches had unique properties, among which white kidney bean starch (KBS) had a high amylose content (43.48%), and its structure was better ordered. Scanning electron microscopy revealed distinct granular morphologies for the three starches. KBS and chickpea starch (CHS) were medium-granular starches, whereas tiger nut starch was a small granular starch. Fourier transform infrared spectroscopy analysis confirmed the absence of significant differences in functional groups and chemical bonds among the three starch molecules. In vitro digestibility studies showed that CHS is more resistant to enzymatic degradation. Overall, these results will facilitate the development of products based on the separation of nonconventional starches from waste.

Moth Bean (Vigna aconitifolia) Starch: Properties, Modifications and Applications—A Review

ABSTRACTMoth bean (Vigna aconitifolia) is considered an underutilized legume but has drawn considerable attention to researchers in recent years owing to its good nutritional value, lower glycemic index, and numerous health benefits. Despite the dietary importance of mung bean as well as starch, the starch extraction processes from moth bean seeds remain inadequately understood. This review discusses recent developments in starch extractability, physico‐chemical and structural characteristics, and different food and non‐food application to utilize moth bean as potential starch source. The starch properties depend mainly on its composition (amylose and amylopectin) and branched chains distribution. Moth bean starch (MBS) exhibited around 15% amylose content and a typical C‐type crystalline structure with granules of diverse sizes and shapes. Different studies exhibited a positive correlation among amylose content and different characteristics of MBS including pasting profile, thermal properties, and its cooking quality. The modification of MBS has been done using some chemical treatments for functionality improvement. MBS possesses good techno‐functional properties that can be exploited for different food product development and non‐food applications. Owing to the promising potential exhibited by native and modified MBS, these can be explored as valuable functional ingredients in various food and non‐food products. Finally, an outlook on potential utilization of MBS in the future is given.

Effects of Premna microphylla turcz polysaccharide on rheological, gelling, and structural properties of mung bean starch and their interactions

The aim of this study was to investigate the effects of Premna microphylla turcz polysaccharide (PMP) on the rheological, gelling, and structural properties of mung bean starch (MBS) and their potential interaction mechanism. Results showed that the addition of PMP significantly improved the pasting properties, rheological properties, water holding capacity, and thermostability of MBS. The texture tests showed a decrease in hardness, gumminess and chewiness, indicating the retrogradation of MBS was inhibited. Scanning electron microscopy (SEM) suggested the MBS-PMP composite gels expressed a denser microstructure with obvious folds and tears. Moreover, the results of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and interaction force tests revealed the main forces between MBS and PMP were hydrogen bonds and hydrophobic interactions to form composite gels with great gelling properties. These results facilitate the practical application of MBS and PMP, and provide some references for understanding the interaction mechanism between starch and polysaccharide.

Effect of combined freezing with heat-moisture treatment (HMT) on the modification of in vitro digestibility, morphostructural, physicochemical, and thermal properties of Adzuki bean starch

Effect of combined freezing with heat-moisture treatment (HMT) on the modification of in vitro digestibility, morphostructural, physicochemical, and thermal properties of Adzuki bean starch

Extraction, characterization, and utilization of mung bean starch as an edible coating material for papaya fruit shelf-life enhancement.

This research was aimed to investigate the utilization of mung bean starch as an innovative edible coating material to enhance the shelf-life of cut papaya fruits. The study focused on the extraction process of mung bean starch and its subsequent characterization through various analyses. Particle size (142.3 ± 1.24 nm), zeta potential (-25.52 ± 1.02 mV), morphological images, Fourier transform infrared (FTIR) spectra, and thermal stability (68.36 ± 0.15°C) were assessed to determine the mung bean starch properties. The functional properties, such as bulk density (0.51 ± 0.004 g/cm3) and tapped density (0.62 ± 0.010 g/cm3), angle of repose (21.61°), swelling power (12.26 ± 0.25%), and minimum gelation concentration (4.01 ± 1.25%), were examined to detect its potential as a coating base material. Subsequently, the prepared mung bean starch coating solution (1%, 2%, 3%, 4%, and 5%) was applied to papaya fruits and the coated fruits' physicochemical characteristics evaluated during storage. These characteristics encompassed color, weight loss, pH shifts, total soluble solids, titratable acidity, vitamin C content, fruit firmness, microbial analysis, and sensory attributes. The results revealed that starch coating on papaya maintained its color, reduced weight loss, preserved vitamin C, and delayed firmness loss, enhancing shelf-life when compared to control sample. These findings demonstrated the effectiveness of mung bean starch coatings in preserving papaya fruits. The research made a significant contribution to the use of mung bean starch as a potential coating material for improving the shelf-life of papaya fruits. This finding has great promise for the field of food preservation and quality control.

Effect of osmotic pressure and simultaneous heat-moisture phosphorylation treatments on the physicochemical properties of mung bean, water caltrop, and corn starches

This study aimed to investigate the physicochemical properties of modified starch prepared through the simultaneous heat-moisture and phosphorylation treatment (HMPT) and osmotic pressure treatment (OPT) for water caltrop starch (WCS), mung bean starch (MBS), and amylose-rich corn starch (CS) for different time periods. Furthermore, variations in starch content [amylose and resistant starch (RS)], swelling powder (SP), water solubility index (WSI), crystallinity, thermal properties, gelatinization enthalpy (ΔH), and glycemic index (GI) were examined. This study demonstrates that neither HMPT nor OPT resulted in a significant increase in the resistant starch (RS) content, whereas all samples succeeded in heat-treating at 105 °C for another 10 min exhibited a significant increase in RS content compared to their native counterparts. Moreover, the gelatinization temperatures of the three starches increased (To, Tp, and Tc), whereas their gelatinization enthalpy (ΔH) and pasting viscosity decreased. In particular, the GI of all three modified starches subjected to HMPT or OPT showed a decreasing trend with modification time, with OPT exhibiting the best effect. Therefore, appropriate modification through HMPT or OPT is a viable approach to develop MBS, WCS, and CS as processed foods with low GI requirements, which exceptionally may be suitable for canned foods, noodles, and bakery products.

Physicochemical characteristics of tannia cocoyam (Xanthosoma sagittifolium) corm flour compared to flours and starches of other grains and tubers

Tannia cocoyam corm is a less popular pantropical carbohydrate source, whereas it has the potential to support food and nutrition security, especially in dry areas. Processing tannia cocoyam corm into flour can increase its use in various food products. This study aimed to determine the yield, nutrition, color, tap bulk density, water absorption capacity (WAC), oil absorption capacity (OAC), swelling capacity, and emulsion activity and stability of tannia cocoyam corm flour compared to sago starch, cassava flour and starch, mung bean starch, corn starch, wheat flour, rice and glutinous rice flours, and potato flour. The significant characteristics of tannia cocoyam corm flour were rather low in calories (dry basis), relatively high in WAC and swelling capacity, low in OAC, and showed emulsion activity and stability. Based on these data, it is suitable for use in mixed and processed food products that require juicy, elastic, and volume expansion characteristics; recommended for food products that are lower in calories and oil content; and can also help to maintain viscosity and form emulsions.

Effects of heat-moisture treatment duration and bean condition on the physicochemical properties of red bean (Phaseolus vulgaris L) starch

Phaseolus vulgaris L (red beans) contain starch that is primarily composed of 44.8% amylose and 55.2% amylopectin but exhibit temperature instability despite their dietary prevalence. This study used Heat Moisture Treatment (HMT) to improve starch attributes in red beans, testing the effects of 4, 5, and 6-hour treatments at 110°C on both fresh and dried samples. The results indicated that both the HMT duration and the beans’ initial state significantly affected the starch’s properties. For fresh beans, the moisture content was reduced from 11.56%±0.54 to 3.04%±0.18, while in dried beans, it decreased from 9.32%±0.94 to 3.81%±0.04. Ash content changed minimally ranging from 1.14%±0.25 to 1.96%±0.02. Bulk density in native fresh and dried starches initially recorded at 0.71 g/mL±0.01 and 0.81 g/mL±0.01, respectively, was observed to decrease after HMT, particularly to 0.51 g/mL±0.01 for dried beans treated for 4 hours. Tapped density varied slightly, reaching up to 0.86 g/mL±0.04. Water activity in both bean types also reduced to 0.42±0.01. In conclusion, HMT significantly enhances Phaseolus vulgaris L starch by lowering moisture, bulk density, and water activity, while maintaining ash content. Further studies should explore the best HMT duration and examine the modified starch’s functional attributes.