Bead Mill Research Articles

Cell Disruption and Hydrolysis of Microchloropsis salina Biomass as a Feedstock for Fermentation

Microalgae are a promising biomass source because of their capability to fixate CO2 very efficiently. In this study, the potential of Microchloropsis salina biomass as a feedstock for fermentation was explored, focusing on biomass hydrolysis by employing various mechanical and chemical cell disruption strategies in combination with enzymatic hydrolysis. Among the mechanical cell disruption methods investigated on a lab scale, namely ultrasonication, bead milling, and high-pressure homogenization, the most effective was bead milling using stainless-steel beads with a diameter of 2 mm. In this way, 87–97% of the cells were disrupted in 40 min using a mixer mill. High-pressure homogenization was also effective, achieving 86% disruption efficiency after four passes on a 30–200 L scale using biomass with 15% (w/w) solids content. Enzymatic hydrolysis of the disrupted cells using a mixture of cellulases and mannanases yielded up to 25% saccharification efficiency after 72 h. Acidic hydrolysis of undisrupted cells followed by enzymatic treatment yielded around 30% saccharification efficiency but was coupled with significant dilution of the resulting hydrolysate. Microalgal biomass hydrolysate produced was determined to have ~8.1 g L−1 sugars and 2.5% (w/w) total carbon, as well as sufficient nitrogen and phosphorus content as a fermentation medium.

Streamlining process development and scale-up: Risk assessment to reduce workload in primary protein recovery

Risk assessment is an integral aspect of the Quality-by-Design strategy to identify potential obstacles at every stage of biopharmaceutical production, from process development to tech transfer. We explored flow process chart, root cause analysis, and failure mode and effects analysis, to assess the scale-up of bacterial cell disruption and its influence on centrifugation and filtration steps. The Ishikawa diagram suggests that data on the impact of homogenizer valve design on product release, impurity profile, particle size distribution, viscosity, and dsDNA fragment size are missing which were collected experimentally for this study. Cell lysates from micro-, lab- and pilot scales cell disruption were analyzed for the above-mentioned parameters. Process parameters affecting these output parameters were identified on each individual scale. Cell disruption on the micro scale was performed in a bead mill. High pressure homogenization was used on lab- and pilot scales. Cell disintegration by bead milling delivers homogenates of product and impurity content comparable to those on bench scale but with 3-fold higher viscosity and significantly larger dsDNA fragments, 8.0 instead of 1.0 kbp, respectively. Miniaturized pressure flow curves identified dsDNA fragment sizes as critical for filter performance during clarification. Combining risk assessment, micro scale cell disintegration and bench scale pressure flow curves allows for selective and efficient process development, and scale up for primary recovery steps.

Formulation and analysis of acrylic emulsion coatings with chrome yellow, phthalocyanine blue, and red oxide pigments using high speed disperser and bead mill techniques

A major commercial problem is achieving stable and fine particle pigment dispersion, especially for aqueous pigment dispersions. It is essential to optimize dispersants in order to moisten, disperse, and stabilize pigments throughout the milling process. This study focuses on formulating acrylic emulsion coatings using chrome yellow, phthalocyanine blue, and red oxide pigment concentrates. The formulations were developed using both high-speed dispersers (HSD) and bead mills. A quality evaluation of the products from the two milling techniques was carried out. Formulations made with chrome yellow, phthalocyanine blue, and red oxide pigment concentrates in a water-based, binder-free system using bead mills, Tween-80, SLS, and cocosulphosuccinate surfactants showed narrower particle size distributions and stronger color than those made with HSD. Specifically, formulations prepared with bead mills and the aforementioned surfactants demonstrated superior color strength compared to those prepared with HSD. The color shade of acrylic emulsion coatings formulated with phthalocyanine blue and red oxide pigment concentrates, along with Tween-80 and cocosulphosuccinate surfactants using HSD, matched the standard color shade formulations prepared in bead mills. However, formulations using phthalocyanine blue pigment concentrates with SLS and oleylsulphosuccinate surfactants in HSD did not match the standard color shade prepared in bead mills.

Ocean Plastics: Extraction, Characterization and Utilization of Macroalgae Biopolymers for Packaging Applications

This review details the extraction, characterization and utilization of seaweed-derived biopolymers for future packaging applications. The review is contextualized within the broader scope of the challenge of plastic pollution and the current urgent need for more sustainable packaging materials. Macroalgae (or seaweed) has been highlighted as a promising source of biopolymers, most commonly sodium alginate, agar and carrageenan, for reasons such as a rapid growth rate and decreased environmental impact when compared with terrestrial plant life. Extraction methods detailed include traditional solvent-based extraction and more sustainable developments such as ultrasound-assisted extraction, microwave-assisted extraction and bead milling. This review additionally presents the characterization techniques most pertinent in determining the applicability of these biopolymers in packaging applications. Properties of key importance to the development of sustainable packaging materials such as thermal properties, mechanical strength, barrier properties and biodegradability are highlighted in comparison to conventional petroleum-based plastics. This review concludes by realistically identifying the challenges faced by implementing seaweed-based biopolymers into packaging structures, such as cost-effectiveness, scalability and performance while suggesting future directions to mitigate these issues and improve the commercial viability of these materials for the packaging industry.

Dexamethasone nanocrystals-embedded hydroxypropyl methylcellulose hydrogel increases cochlear delivery and attenuates hearing loss following intratympanic injection

Herein, dexamethasone (DEX) nanocrystalline suspension (NS)-embedded hydrogel (NS-G) was constructed using a hydroxypropyl methylcellulose (HPMC) polymer to enhance cochlear delivery and attenuate hearing loss following intratympanic (IT) injection. Hydrophobic steroidal nanocrystals were prepared using a bead milling technique and incorporated into a polysaccharide hydrogel. The NS-G system with HPMC (average molecular weight, 86,000 g/mol; 15 mg/mL) was characterized as follows: rod-shaped drug crystalline; particle size <300 nm; and constant complex viscosity ≤1.17 Pa·s. Pulverization of the drug particles into submicron diameters enhanced drug dissolution, while the HPMC matrix increased the residence time in the middle ear cavity, exhibiting a controlled release profile. The IT NS-G system elicited markedly enhanced and prolonged drug delivery (> 9 h) to the cochlear tissue compared with that of DEX sodium phosphate (DEX-SP), a water-soluble prodrug. In mice with kanamycin- and furosemide-induced ototoxicity, NS-G markedly enhanced hearing preservation across all frequencies (8–32 kHz), as revealed by an auditory brainstem response test, compared with both saline and DEX-SP. Moreover, treatment with NS-G showed enhanced anti-inflammatory effects, as evidenced by decreased levels of inflammation-related cytokines. Therefore, the IT administration of DEX NS-loaded HPMC hydrogels is a promising strategy for treating hearing loss.

Protein extraction from seaweed Saccharina latissima with deep eutectic solvents

Interest in seaweed as a sustainable source of protein is growing, and deep eutectic solvents (DESs) are a promising green alternative with proven efficacy in protein extraction. This work studies the selective extraction of protein towards carbohydrates from the brown algae Saccharina latissima using DESs. Eleven DESs based on choline chloride (ChCl) and betaine were tested for freeze-dried biomass. The four DESs with the best performance (ChCl:Oxalic acid, ChCl:2Urea, ChCl:2Levulinic acid and Betaine:2Urea:Water) were subsequently used to investigate the effect of temperature (20, 30 and 40 °C) and the addition of water (0 and 47 %) on the extraction process. Betaine:2Urea:Water at 1 h, 40 °C, and 47 % added water provided the highest protein recovery yield (10.6 %) while minimizing carbohydrate extraction (1.3 %). These results demonstrated similar protein recovery yields to a benchmark process using bead milling, but with better selectivity. Thus, this work provides a sustainable extraction method for the recovery of proteins from seaweed and open new alternatives combining both approaches.

Sustainable biorefining of Chlorella vulgaris into protein, lipid, bioethanol, and biogas with substantial socioeconomic benefits

An integrated biorefinery was developed that utilizes microalgal biomass, Chlorella vulgaris, to sustainably produce proteins, fatty acids, bioethanol, and biogas. The microalgal soluble proteins and fatty acids were initially extracted through a cascading extraction process, including bead milling and solvent extraction. Subsequently, the investigation focused on utilizing the biomass residues for bioethanol and biogas production, ultimately improving energy recovery. Implementing the cascading process resulted in a 25 % enhancement in bioethanol yield and a 22.4 % increase in biomethane yield compared to untreated biomass. This approach resulted in 78.0 g of protein, 50.9 g of lipid, 20.8 ml of ethanol, and 136.5 L of methane from one kilogram of dry C. vulgaris biomass. Considering the potential of 8,640 k tons of annual microalgae production in Iran, an estimated 4.1 million tons of CO2 emissions could be averted. This reduction could result in saving approximately 1394.8 million USD in associated social costs of carbon. These improvements in fully valorizing biomass through practical cascading methods significantly advance microalgal biorefinery.

Exploring the relationship between bulk Young’s Modulus of materials and milling efficiency during wet bead milling of pharmaceutical compounds

Wet bead milling (WBM) is one of the main approaches for manufacturing long acting injectable (LAI) suspensions, wherein the particle size of an Active Pharmaceutical Ingredient (API) is reduced in a liquid vehicle via grinding. A common challenge observed during WBM is long milling time to achieve target particle size, resulting in poor milling efficiency. The objective of this work was to identify potential API attributes predictive of milling efficiency during WBM. In this study, physical and mechanical properties of nine APIs were characterized. Formulations with these APIs were manufactured using WBM. Bulk Young’s Modulus was identified to have a significant influence on the rate of particle attrition. The rank order of Young’s Moduli of the APIs was consistent with that of milling efficiency, estimated by an empirical function defined in this study called Milling Resistance (ϕ), representing the holistic impact of milling time, tip speed, bead loading, and batch to chamber volume ratio. The identification of such intrinsic material properties, which provide an early evaluation of potential manufacturing risks, is beneficial to product development, as these assessments can be performed with limited quantities of materials and help identify and design out scale-up challenges.

Improving protein extraction and peptide production from Chlorella vulgaris using combined mechanical/physical and enzymatic pre-treatments

Chlorella vulgaris is a microalga rich in proteins with potential applications in food and feed industries. However, the presence of a cellulose-containing cell wall, which is a major barrier to protein extraction, together with fibroproteinaceous complexes, limits the bioaccessibility of nutritional and bioactive proteins and peptides from C. vulgaris biomass. Therefore, this study aimed to evaluate the effect of different mechanical/physical pre-treatments (bead milling, extrusion, freeze-drying, heating, microwave and sonication) combined or not with enzymatic treatments (commercial trypsin and pancreatin) on protein extraction and peptide formation from a C. vulgaris suspension. The amount of total protein and peptides released to the supernatant was quantified by Bradford and o-phthaldialdehyde assays, respectively. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis was used to analyse the extracted protein fractions. The results showed that extrusion caused a 3-fold increase in total peptides (p < 0.001) compared to no-pretreatment, and trypsin increased peptides formed in bead-milled (p = 0.020) and freeze-dried (p = 0.021) microalga relative to those pre-treatments alone. Some pre-treatments, such as bead milling and microwave, were effective in releasing specific protein fractions, particularly those from 32 to 40 kDa (up to 1.2-fold), compared to control. Pancreatin combined with bead milling decreased 32 to 40 kDa- and 26 kDa-protein fractions (p < 0.010) compared with the sole use of mechanical treatment, whereas the same enzyme mixture associated with microwave produced a similar result for 26 kDa-protein fraction (p = 0.023). Pancreatin also effectively reduced the total protein fraction released after pre-treatment with sonication (p = 0.013). These findings suggest that combining different pre-treatments and enzymatic treatments could improve protein extraction from C. vulgaris biomass, providing a useful approach for the development of sustainable protein sources. The present results highlight the need for further studies to assess the efficacy of extrusion in improving the bioaccessibility of C. vulgaris proteins in monogastric animals' diets.

Integrating bead milling and alkaline solubilization for enhanced protein recovery from microalgae: A comprehensive approach

Microalgae, Chlorella vulgaris exhibits substantial potential as a sustainable food ingredient, but its robust cell wall and limited protein solubility hinder industrial scale protein recovery. A comprehensive solution was devised, integrating dry bead milling and alkaline solubilization. In this method, cells were initially disrupted with bead milling followed by alkali (NaOH) treatment. Without bead milling, protein extraction yield was very low (5.0 % with water, 16.8 % with 0.1 M NaOH) at a biomass loading of 20 g/L. However, the integrated approach significantly improved these results, achieving a maximum protein extraction yield of about 47.3 % at a biomass loading of 100 g/L. The optimized conditions for both dry bead milling (frequency 26 Hz, duration 1.0 h) and alkaline solubilization (biomass weight to NaOH molar ratio 200–250 (g/M), 37 °C, mixing time 1.0 h) played a pivotal role in realizing these improved results. This integrated approach effectively addresses the challenges and holds industrial relevance, offering a more efficient way to extract microalgal protein.

Preparation of fine suspensions using stirred media bead mill

Preparation of fine suspensions using stirred media bead mill

Validation study on a coarse-grained DEM-CFD simulation in a bead mill

A bead mill is often employed in powder processes. In the bead mill, the bead-particles move rapidly under high shear rate due to the moving structures. The discrete element method (DEM) coupled with the computational fluid dynamics (CFD) has been applied to simulate the bead-particle behavior for the optimization of the bead mill design and operating conditions. However, the existing DEM-CFD method faces essential problems regarding the substantial limit of the number of computational particles, stable calculations, and modeling of rapidly moving structures. To solve these problems, we newly investigate a numerical model combining the coarse-grained DEM-CFD, an implicit algorithm for the drag force term, and a scalar field-based wall boundary in the calculation of a bead mill commonly employed in industrial powder processes. In this study, our proposed model is shown to successfully simulate the macroscopic behavior of the bead-particles through the validation test of the coarse-grained DEM-CFD simulation in the bead mill system. Specifically, the macroscopic characteristics such as the particle location, the particle velocity, and total kinetic energy, are shown to agree well between the original particle system and coarse-grained particle systems. Consequently, this study illustrates the combination of the coarse-grained DEM-CFD, an implicit algorithm for the drag force term, and the scalar field-based wall boundary is essential for simulating the typically industrial bead mill, where an enormous number of bead-particles should be calculated in liquid under high shear rate.

개선된 고상 합성법을 통한 LiNi 0.5 Mn 1.5 O 4 양극재 연구

In this study, LiNi 0.5 Mn 1.5 O 4 was synthesized from various raw materials, including NiO, Ni 2 O 3 , MnO 2 , and Mn 3 O 4 . An improved solid-phase synthesis method was then used to reduce impurities through uniform mixing via bead milling and to achieve a consistent secondary particle shape and distribution through spray drying. The LiNi 0.5 Mn 1.5 O 4 was obtained by selecting materials optimized for rate capability and by applying an improved synthesis process. X-ray diffraction and scanning electron microscopy analyses confirmed that LiNi 0.5 Mn 1.5 O 4 successfully synthesized primary and secondary particles. This result showed that LiNi 0.5 Mn 1.5 O 4 , when subjected to an improved solid-phase synthesis, reduces production costs ,enhances its commercial value, and can be applied to lithium-ion secondary batteries to achieve high energy density and high voltage. In short, LiNi 0.5 Mn 1.5 O 4 can be used as an efficient energy storage material.

Surface modification of micronized calcite (CaCO3) with stearic acid via mechanical dry coating

In this study, the surface coating (modification) of micronized calcite (CaCO3, d50=135 μm) in a stirred media (bead) mill with stearic acid was investigated. As a result of 15 min grinding and 5 min coating experiments, a product with d50=2.15 μm and specific surface area of 3.21 m2/cm3 was obtained. The activation rate of this final product as an indicator of coating rate was 97.7%. According to the optimum coating results, the stearic acid rate was determined as 1%, the modification time was 7.5 min, the ball load was 900 g and the stirring speed was 600 rpm.

Development of a Semi-Mechanistic Modeling Framework for Wet Bead Milling of Pharmaceutical Nanosuspensions.

This study aimed to develop a practical semi-mechanistic modeling framework to predict particle size evolution during wet bead milling of pharmaceutical nanosuspensions over a wide range of process conditions and milling scales. The model incorporates process parameters, formulation parameters, and equipment-specific parameters such as rotor speed, bead type, bead size, bead loading, active pharmaceutical ingredient (API) mass, temperature, API loading, maximum bead volume, blade diameter, distance between blade and wall, and an efficiency parameter. The characteristic particle size quantiles, i.e., x10, x50, and x90, were transformed to obtain a linear relationship with time, while the general functional form of the apparent breakage rate constant of this relationship was derived based on three models with different complexity levels. Model A, the most complex and general model, was derived directly from microhydrodynamics. Model B is a simpler model based on a power-law function of process parameters. Model C is the simplest model, which is the pre-calibrated version of Model B based on data collected from different mills across scales, formulations, and drug products. Being simple and computationally convenient, Model C is expected to reduce the amount of experimentation needed to develop and optimize the wet bead milling process and streamline scale-up and/or scale-out.

Evaluation of cell disruption methods for protein and coenzyme Q10 quantification in purple non-sulfur bacteria.

A recent focus has been on the recovery of single-cell protein and other nutritionally valuable bioproducts, such as Coenzyme Q10 (CoQ10) from purple non-sulfur bacteria (PNSB) biomass following wastewater treatment. However, due to PNSB's peculiar cell envelope (e.g., increased membrane cross-section for energy transduction) and relatively smaller cell size compared to well-studied microbial protein sources like yeast and microalgae, the effectiveness of common cell disruption methods for protein quantification from PNSB may differ. Thus, this study examines the efficiency of selected chemical (NaOH and EDTA), mechanical (homogenization and bead milling), physical (thermal and bath/probe sonication), and combined chemical-mechanical/physical treatment techniques on the PNSB cell lysis. PNSB biomass was recovered from the treatment of gas-to-liquid process water. Biomass protein and CoQ10 contents were quantified based on extraction efficiency. Considering single-treatment techniques, bead milling resulted in the best protein yields (p < 0.001), with the other techniques resulting in poor yields. However, the NaOH-assisted sonication (combined chemical/physical treatment technique) resulted in similar protein recovery (p = 1.00) with bead milling, with the former having a better amino acid profile. For example, close to 50% of the amino acids, such as sensitive ones like tryptophan, threonine, cystine, and methionine, were detected in higher concentrations in NaOH-assisted sonication (>10% relative difference) compared to bead-milling due to its less disruptive nature and improved solubility of amino acids in alkaline conditions. Overall, PNSB required more intensive protein extraction techniques than were reported to be effective on other single-cell organisms. NaOH was the preferred chemical for chemical-aided mechanical/physical extraction as EDTA was observed to interfere with the Lowry protein kit, resulting in significantly lower concentrations. However, EDTA was the preferred chemical agent for CoQ10 extraction and quantification. CoQ10 extraction efficiency was also suspected to be adversely influenced by pH and temperature.

Nanocrystal Structure of Minoxidil: A Safe Stimulator for Hair Growth Factor for C57BL/6 Mice.

Commercial Minoxidil (MXD) is commonly used as a vasodilator agent of hair follicles for providing direct dermal papilla cell proliferation and consequently enhancing the rate of hair growth. The current study attempted to improve the bioactivity and water solubility of MXD by producing nanocrystal structures and investigating the obtained hair growthstimulating activity on C57BL/6 mice. The MXD nanoparticles (MXD-NPs) were prepared through a bead mill and ultrasonic process and characterized by DLS, XRD, UV-Vis, FTIR, FESEM, TEM, and Zeta-potential techniques. The cytotoxicity of MXD-NPs was studied on human dermal fibroblast (HDF) by MTT assay. Lastly, we analyzed the comparative hair growth inductive activity of certain MXD-NPs concentrations on C57BL/6 mice. The stabled MXD-NPs (-46 mV, 21.9 nm) caused a significant increase in the hair growth rate of C57BL/6 mice by running a safe site-specific delivery mechanism on the targeted pilosebaceous follicles when compared to MXD. The MXD-NPs-receiving mice exhibited a greater rate of anagen/telogen follicular when compared with MXD-treated types, which verified the improvement of their hair re-growing and follicular-stimulative activities. Therefore, these outcomes confirmed the potential of MXD-NPs for substituting its commercial solution format as a safe and efficient iso-formulation structure.

Sugar Cane Vinasse as a Renewable and Sustainable Feedstock for Lipid Production from Aspergillus Niger and Aspergillus flavus

Background and Aims: In the search for sustainability and the use of renewable energies, biodiesel has stood out as a notable alternative to the growing energy demand of modern society. Microbial fat synthesis for lipids has been a possibility to produce this biofuel. In this context, the research work aimed to cultivate Aspergillus niger and Aspergillus flavus fungi in commercial and homemade broth, plus variable concentrations of sugarcane vinasse as a substrate to extract lipids produced using different extraction techniques and solvents.&#x0D; Methodology: The fungi were grown in a commercial medium and a homemade one made with potato extract. After cultivation, they were submitted to two pre-treatments: the Bead Mill technique and the Ultrasound technique. The extraction of lipids produced in a commercial medium was performed via Soxhlet with a mixture of solvents. Therefore, the lipids produced in a homemade medium were extracted with the adapted Bligh-Dyer method.&#x0D; Results: The species grew to the different culture media submitted, but the home culture medium increased the reproductive. The industrial residue proves to be a good alternative as a supplement to the culture medium, helping to reduce costs and preserve the environment. The extraction by Soxhlet showed satisfactory results when the fungi were grown in a commercial medium, with Aspergillus niger being extracted from the ethyl acetate solvent, obtaining a yield of 18.78%, and for A. flavus extracted with dichloromethane/methanol (9:1) and chloroform, bringing yield of 32.98% and 32%, respectively. Although extraction by Soxhlet showed some excellent results, extraction with the Bligh-Dyer method demonstrated better lipid fractions ranging from 25% to 67% and reduced energy expenditure, indicating a promising use, cost, and benefit.&#x0D; Conclusion: Filamentous fungi of the genus Aspergillus can be used to produce biodiesel as they proliferate and can produce the same amount of lipids as a vegetable, which requires a much larger area of soil, for example.

Effect of bead mill dispermat system with specific surfactants on organic pigments

Abstract The study demonstrates the effective application of high-speed dispersion and bead mill dispermat systems in dispersing organic pigments, specifically phthalocyanine blue and green, in water with positive results. Particle size analysis reveals that formulations prepared using a bead mill with the selective surfactants such as Tween-80, SLS, Cocosulphosuccinate (CSPS) and Oleyl sulphosuccinate (OSPS) which exhibits a narrower range of particle size distribution compared to those prepared using high-speed dispersion (HSD). Phthalocyanine blue pigment concentrate formulation, the bead mill preparation using Tween-80 and CSPS surfactants resulted in a higher viscosity and color strength compared to HSD. In addition, the phthalocyanine green pigment concentrate formulation prepared using a bead mill with SLS surfactant showed higher color strength than the formulation prepared using HSD with SLS surfactant. Moreover, the comparative study reported the Nano CaCO3 pigment concentrate formulation prepared using a bead mill with SLS surfactant displayed a narrow range of particle size distribution ranging from 0.31 to 0.56 nm, with an average particle size of 0.435 nm, compared to the formulation prepared using HSD. All these findings suggested bead mill dispermat system with specific surfactants can produce pigment dispersions with a narrower particle size distribution, higher color strength, and better viscosity compared to HSD which demonstrating its potential as a more efficient and effective method for pigment dispersion.

ИССЛЕДОВАНИЕ ВЛИЯНИЯ МНОГОЗАХОДНЫХ ВИНТОВЫХ ЛОПАСТЕЙ НА ЭНЕРГЕТИЧЕСКОЕ СОСТОЯНИЕ МЕЛЮЩИХ ТЕЛ В БИСЕРНОЙ МЕЛЬНИЦЕ

The distribution of bead mills in industrial enterprises of the processing industry for fine and ultra-fine grinding of various materials, the need for their improvement is demonstrated. A new design of a horizontal bead mill with multi-start screw blades is considered. This allows increasing productivity and reducing specific power consumption. The purpose of the studies was to assess the possibility of increasing the efficiency of the bead mill when screw blades are installed on the paddle shaft and their influence on the energy state of grinding bodies. Using the EDEM software product, the mathematical planning method of the experiment, a simulation of the process of movement of grinding bodies in the model of the body part of the bead mill was carried out when it was equipped with a pair of two-way screw blades. The obtained regression equation was studied. It describes the dependence of the averaged value of the total kinetic energy of the grinding body on varying factors: the angle of rotation of the beginning of the blade screw starts, the distance between the screw blades, the pitch of the screw blade, and the angular speed of the blade shaft. The possibilities of increasing the average value of the total kinetic energy of the grinding body and regulating the energy state of the grinding bodies have been established. The feasibility of conducting further theoretical and experimental studies aimed at studying the process of grinding material in a bead mill with multi-start screw blades and establishing rational design and technological parameters that ensure increased efficiency of this process has been determined.