Bathophenanthroline Complex Research Articles

A dual-modal fluorometric and colorimetric nanoprobe based on graphitic carbon nitrite quantum dots and Fe (II)-bathophenanthroline complex for detection of nitrite in sausage and water

A fluorometric and colorimetric dual-mode sensing platform based on graphitic carbon nitrite quantum dots (g-CNQDs) and Fe (II)-bathophenanthroline complex (BPS-Fe2+) was designed to the sensitive detection of nitrite (NO2−) in sausage and water. In this system, the fluorescence of g-CNQDs was quenched by BPS-Fe2+ complex due to the inner filter effect (IFE). When NO2− was present, Fe2+ was oxidized by nitrite to form BPS-Fe3+ complex with BPS, leading to the recovery of the fluorescence from g-CNQDs. Therefore, we constructed a “turn-off-on” fluorescence probe for detection of NO2−. Moreover, with the increase of NO2− concentration, the color of the solution changed from red to colorless, so the UV–vis measurements and on-site visual detection were realized. The method is capable of detecting NO2− in the concentration range of 2.32–34.8 μM with good selectivity and high sensitivity. In addition, the method has the potential to determine NO2− in water samples and sausage samples.

An anticancer Os(II) bathophenanthroline complex as a human breast cancer stem cell-selective, mammosphere potent agent that kills cells by necroptosis

Conventional chemotherapy is mostly effective in the treatment of rapidly-dividing differentiated tumor cells but has limited application toward eliminating cancer stem cell (CSC) population. The presence of a very small number of CSCs may contribute to the development of therapeutic resistance, metastases, and relapse. Thus, treatment failure by developing novel anticancer drugs capable of effective targeting of CSCs is at present a major challenge for research focused on chemotherapy of cancer. Here, we show that Os(II) complex 2 [Os(η6-pcym)(bphen)(dca)]PF6 (pcym = p-cymene, bphen = bathophenanthroline, and dca = dichloroacetate), is capable of efficient and selective killing CSCs in heterogeneous populations of human breast cancer cells MCF-7 and SKBR-3. Notably, its remarkable submicromolar potency to kill CSCs is considerably higher than that of its Ru analog, [Ru(η6-pcym)(bphen)(dca)]PF6 (complex 1) and salinomycin, one of the most selective CSC-targeting compounds hitherto identified. Furthermore, Os(II) complex 2 reduces the formation, size, and viability of three-dimensional mammospheres which more closely reflect the tumor microenvironment than cells in traditional two-dimensional cultures. The antiproliferation studies and propidium iodide staining using flow cytometry suggest that Os(II) complex 2 induces human breast cancer stem cell death predominantly by necroptosis, a programmed form of necrosis. The results of this study demonstrate the promise of Os(II) complex 2 in treating human breast tumors. They also represent the foundation for further preclinical and clinical studies and applications of Os(II) complex 2 to comply with the emergent need for human breast CSCs-specific chemotherapeutics capable to treat chemotherapy-resistant and relapsed human breast tumors.

Application of Iron Bathophenanthroline Complex as a Redox Mediating Agent for Imaging Surface Immobilized DNA Using Scanning Electrochemical Microscopy

Application of Iron Bathophenanthroline Complex as a Redox Mediating Agent for Imaging Surface Immobilized DNA Using Scanning Electrochemical Microscopy

Photophysical Characterization of a FRET System Using Tailor-Made DNA Oligonucleotide Sequences

We have carried out a detailed photophysical study of the FRET D/A pair consisting of a carbostyril donor and a Ru(II)bathophenanthroline complex acceptor in double-stranded synthetic DNA. Altogether 13 different double-stranded 30 base pair DNAs showing small incremental differences in the distances between donor and acceptor were synthesized. Using the fluorescence of the donor as well as of the acceptor, D/A separations were determined and compared to those derived from a well-established model for DNA distance calculations. The model calculations and anisotropy studies revealed that the donor can nearly be seen as a free rotator allowing the application of the established FRET data evaluation.

Fluorescence and Electrochemical Probing of N-Acetylamino Acids, Nucleotides, and DNA by the Eu(III)−Bathophenanthroline Complex

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionORIGINAL ARTICLEThis notice is a correctionFluorescence and Electrochemical Probing of N-Acetylamino Acids, Nucleotides, and DNA by the Eu(III)−Bathophenanthroline ComplexH. A. Azab, S. S. Al-Deyab, Z. M. Anwar, and R. Gh. AhmedCite this: J. Chem. Eng. Data 2010, 55, 11, 5397Publication Date (Web):September 9, 2010Publication History Received31 August 2010Published online9 September 2010Published inissue 11 November 2010https://doi.org/10.1021/je100898qCopyright © 2010 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views208Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (19 KB) Get e-AlertsSUBJECTS:Fluorescence,Genetics,Monomers Get e-Alerts

Fluorescence and Electrochemical Probing of N-Acetylamino Acids, Nucleotides, and DNA by the Eu(III)−Bathophenanthroline Complex

The solid complex Eu(III)−bathophenanthroline was synthesized and characterized by elemental analysis, IR spectra, and thermal analysis. The interaction of the Eu(III)−bathophenanthroline solid complex with calf-thymus DNA has been investigated by fluorescence and electrochemical methods including cyclic voltammetery and differential pulse polarography on a glassy carbon electrode. The formation of binary and ternary complexes of Eu(III) with nucleotides guanosine 5′- monophosphate (5′-GMP), adenosine 5′-monophosphate (5′-AMP), inosine 5′-monophosphate (5′-IMP), cytidine 5′-monophosphate (5′-CMP), or N-acetylamino acids (N-acetylaspartic acid, N-acetylhistidine, and N-acetylhistamine), and bathophenanthroline (BPhen) has been studied potentiometrically at (25.0 ± 0.1) °C and an ionic strength of I = 0.1 mol·dm−3 (KNO3) in 1.8 % v/v ethanol−water mixture solvent. The formation of the normal and protonated binary and ternary complexes is inferred from the corresponding titration curves. The experimental con...

Quenching of the long-lived Ru(ii)bathophenanthroline luminescence for the detection of supramolecular interactions

A feasibility study based on tailor-made peptide sequences for a new robust luminescence probe-system using the long-lived luminescence of a Ru(II)-bathophenanthroline complex in combination with an efficient anthraquinone-type quencher is presented. Due do their high chemical stability, both dyes can be introduced during solid-phase peptide synthesis avoiding post-synthetic labelling. Photophysical measurements revealed an intense quenching of the luminescence of the Ru-complex (65-68%) which was also confirmed by calculations resulting from decay time measurements. The long-lived luminescence allows for a time-gated detection scheme, which can reduce any luminescence contribution from matrix components.

A New Robust and Highly Sensitive FRET Donor–Acceptor Pair: Synthesis, Characterization, and Application in a Thrombin Assay

AbstractThe synthesis of a new, robust fluorescence‐resonance‐energy‐transfer (FRET) system is described. Its donor chromophore is derived from an N‐allyl‐substituted quinolinone attached to 4‐bromophenylalanine via Heck cross‐coupling. The resulting Fmoc‐protected derivative 11 was used as building block in solid‐phase peptide synthesis (SPPS). As FRET acceptor, a sulfonylated ruthenium(II)–bathophenanthroline complex with a peripheral COOH function was prepared for covalent attachment to target molecules. The UV/VIS absorption and emission spectra of peptides bearing only the donor (D) or acceptor (A) dye showed a good overlap of the emission band of the donor with the absorption band of the acceptor. The fluorescence spectra of a peptide bearing both dyes revealed an additional emission after excitation of the donor, which is due to indirect excitation of the acceptor via FRET. The long fluorescence lifetime of the RuII complex (0.53 μs) makes it well‐suited for time‐resolved measurements. As a first application of this new FRET system, the peptide 18, with the recognition sequence for the protease thrombin, flanked by the two dyes, was synthesized and successfully cleaved by the enzyme. The change in the ratio of the fluorescence intensities could be determined.

Solid Phase Extraction and Simultaneous Spectrophotometric Determination of Trace Amounts of Copper and Iron Using Mixture of Ligands

A novel sensitive and simple method for rapid and selective extraction, preconcentration and determination of iron (as its bathophenanthroline complex) and copper (as its neocuproine complex) using octadecyl silica cartridges and dual wavelength spectrophotometry is presented. The dual wavelength method (533 nm for the iron-bathophenanthroline and 454 nm for the copper-neocuproine as the analytical wavelength) is used to eliminate spectral interferences. Extraction efficiency and the influence of flow rates of sample solution and eluent, pH, amount of neocuproine, bathophenanthroline and hydroxylamine hydrochloride, type and least amount of eluent for elution of iron and copper complexes from cartridge, break-through volume and limit of detection are evaluated. The effects of various cationic and anionic interferences on percent recovery of iron and copper are also studied. Extraction efficiencies >95% are obtained by elution of cartridges with minimal amount of organic solvent. Iron and copper were determined in the range of 3–100 ng mL−1. The limits of detection are 0.98 and 1.13 ng mL−1 for iron and copper, respectively. The proposed method is applied successfully to the determination of both analytes in river, tap and well water samples.

Solid-Phase Extraction, Separation, and Visible Spectrophotometric Determination of Trace Amounts of Iron in Water Samples

A simple and reliable method is presented for the rapid extraction, separation, preconcentration, and determination of iron as its bathophenanthroline complex by the use of octadecylsilica membrane disks and spectrophotometry. We evaluted extraction efficiency, the influence of sample matrix, type and optimum amount of extractant, flow rates of sample solution and eluent, pH, amounts of bathophenanthroline and hydroxylamine hydrochloride, breakthrough volume, and limit of detection. We also studied the effects of various cationic interferences on percent recovery of iron. Complete elution of the complex from disks was obtained with a minimal amount of solvent. The limit of detection of the proposed method is 0.080 ppb. The method was applied to the recovery and determination of iron in natural waters.

Direct spectrophotometric determination of ferbam (Iron(III) dimethyldithiocarbamate) in commercial sample and wheat grains using 4,7-diphenyl-1,10-phenanthroline.

A procedure has been developed for the determination of iron(III) dimethyldithiocarbamate by converting it into the iron(II)-bathophenanthroline complex, which is then dissolved in acetone-water (1:1), and the absorbance is measured at 534 nm against a reagent blank. Beer's law is obeyed over the concentration range 0.5-20 microg x mL(-1) in the final solution. The method is sensitive and highly selective and is used for the direct determination of ferbam in a commercial sample and in mixtures with various dithiocarbamates (ziram, zineb, maneb, etc.) and from wheat grains.

Green, catalytic oxidation of alcohols in water

Alcohol oxidations are typically performed with stoichiometric reagents that generate heavy-metal waste and are usually run in chlorinated solvents. A water-soluble palladium(II) bathophenanthroline complex is a stable recyclable catalyst for the selective aerobic oxidation of a wide range of alcohols to aldehydes, ketones, and carboxylic acids in a biphasic water-alcohol system. The use of water as a solvent and air as the oxidant makes the reaction interesting from both an economic and environmental point of view.

Spectrophotometric determination of ferbam (iron(iii) dimethyldithiocarbamate) in commercial sample and wheat grains using 4,7-diphenyl-1,10-phenanthroline after extraction into mesityl oxide

A procedure has been developed for the determination of iron(III) dimethyldithiocarbamate by converting it into iron(II)-bathophenanthroline complex, which is then extracted into mesityl oxide in the presence of potassium perchlorate. The absorbance is then measured at 534 nm against a reagent blank. Beer's law is obeyed over the concentration range 0.5-20 microg ml(-1) in the final solution. The method is sensitive, highly selective and can be used for the determination of ferbam in a commercial sample, and in mixtures with various dithiocarbamates (ziram, zineb, maneb, etc.) and in wheat grains.

Spectrophotometric determination of ferbam (iron(III) dimethyldithiocarbamate) in a commercial sample and wheat grains using 4,7‐diphenyl‐1,10‐phenanthroline

A procedure has been developed for the determination of iron(III) dimethyldithiocarbamate by converting it into iron(III)- bathophenanthroline complex, which is then dissolved in water in the presence of sodium dodecyl sulphate as a surfactant, and the absorbance measured at 532 nm against a reagent blank. Beer's law is obeyed over the concentration range 0.3–20 µg ml−1 in the final solution. The method is sensitive, highly selective and can be used for the determination of ferbam in a commercial sample, in mixtures with various dithiocarbamates (ziram, zineb, maneb, etc.) and from wheat grains. © 1999 Society of Chemical Industry

Spectrophotometric determination of ferbam (iron(III) dimethyldithiocarbamate) in a commercial sample and wheat grains using 4,7-diphenyl-1,10-phenanthroline

A procedure has been developed for the determination of iron(III) dimethyldithiocarbamate by converting it into iron(III)- bathophenanthroline complex, which is then dissolved in water in the presence of sodium dodecyl sulphate as a surfactant, and the absorbance measured at 532 nm against a reagent blank. Beer's law is obeyed over the concentration range 0.3-20 μg ml -1 in the final solution. The method is sensitive, highly selective and can be used for the determination of ferbam in a commercial sample, in mixtures with various dithiocarbamates (ziram, zineb, maneb, etc.) and from wheat grains.

Inhibition of mitochondrial F1 ATPase and sarcoplasmic reticulum ATPase by hydrophobic molecules

The hydrophobic nature of the active site of two energy-transducing ATPases was explored by comparing interactions between Pi and each of three hydrophobic drugs in the absence and presence of organic solvents. The drugs tested were the Fe . bathophenanthroline complex and the anticalmodulin drugs, calmidazolium and trifluoperazine. All inhibit the Pi in equilibrium with ATP exchange reaction catalyzed by submitochondrial particles and the ATPase activity of both submitochondrial particles and soluble F1 ATPase. The inhibition by the three drugs is reversed by either raising the Pi concentration or by adding organic solvent (dimethylsulfoxide, ethyleneglycol or methanol) to the medium. The inhibition of the Pi in equilibrium with ATP exchange by trifluoperazine becomes more pronounced when the electrochemical proton gradient formed across the membrane of the submitochondrial particles is decreased by the addition to the medium of the proton ionophore carbonylcyanide p-trifluoromethoxyphenylhydrazone. The ATPase activity and the Ca2+ uptake by sarcoplasmic reticulum vesicles are inhibited by the Fe . bathophenanthroline complex, calmidazolium and trifluoperazine. Phosphorylation of the ATPases by Pi, synthesis of ATP from ADP and Pi and the fast efflux of Ca2+ observed during reversal of the Ca2+ pump are inhibited by the three drugs. The inhibition is reversed by raising the concentration of Pi or dimethylsulfoxide. The three drugs tested appear to compete with Pi for a common binding site on the Ca2+-ATPase. The data presented are interpreted according to the proposal that the catalytic site of an enzyme involved in energy transduction undergoes a hydrophobic-hydrophilic transition during the catalytic cycle.

Determination of specific activity of iron-55 by spectrophotometry and liquid scintillation counting with bathophenanthroline complex

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of specific activity of iron-55 by spectrophotometry and liquid scintillation counting with bathophenanthroline complexChushiro. Yonezawa, Michio. Hoshi, and Enzo. TachikawaCite this: Anal. Chem. 1985, 57, 14, 2961–2965Publication Date (Print):December 1, 1985Publication History Published online1 May 2002Published inissue 1 December 1985https://doi.org/10.1021/ac00291a047RIGHTS & PERMISSIONSArticle Views112Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (647 KB) Get e-Alerts Get e-Alerts

Determination of iron in high purity gallium arsenide, gallium and arsenic as the bathophenanthroline complex

A spectrophotometric method is described for the determination of iron in high purity GaAs and As or Ga, having a limit of detection of 3.5×10−5% and 7×10−5% Fe, resp. After sample dissolution and pre-reduction of Fe3+ to the bivalent state with hydroxylammonium chloride the red iron(II)-bathophenanthroline complex is formed in the presence of sodium citrate, ascorbic acid and perchlorate. It is extracted into chloroform and optical density of the resulting extract is measured at 533 nm. The effect of copper is discussed. The procedure is stated to be rapid and applicable not only to Ga, As and GaAs, but also to other materials.

Extraction and determination of iron as the bathophenanthroline complex in high-purity niobium, tantalum, molybdenum and tungsten metals

A spectrophotometric method for determining iron in the range 0·001–0·125% in high-purity niobium, tantalum, molybdenum and tungsten metals is described. After sample dissolution and reduction of iron to the bivalent state with ascorbic acid and hydroxylamine hydrochloride, the red complex formed between iron II and bathophenanthroline (4,7-diphenyl-1,10-phenanthroline) is extracted into n-arnyl alcohol and the absorbance of the resulting extract is determined at 536 mμ. Interference from copper is eliminated with thiourea. Cobalt, cadmium, nickel, manganese and zinc also interfere but the amounts of each of these impurities present in the four high-purity metals described are so low that their interference effects are negligible in the proposed method. Highly reproducible and precise results can be obtained with careful control of the pH during reduction and extraction.