Growth Of Basil Research Articles

Synergistic Effect of Organic Inputs on the Morphophysiological Traits of Sweet Basil (Ocimum basilicum L.)

The present investigation was conducted from 2023 in Chengam, Tiruvannamalai district. The objective was to evaluate the impact of various organic treatments on the growth and yield characteristics of sweet basil (Ocimum basilicum L.). The experiment was carried out is Randomized Block Design with ten treatments and replication thrice. Among the ten different treatments applied, the combination of FYM @ 25 t ha⁻1 + VC @ 2.5 t ha⁻1 + Seaweed extract @ 2% (T9) consistently outperformed other treatments. This treatment significantly enhanced growth characteristics viz., plant height (35.01, 60.33, 86.71 and 110.34 cm), number of leaves plant-1(291.34, 540.56, 850.41 and 1010.66), leaf area (482.33, 1673.20, 3581.23 and 4468.67 cm²) and leaf area index (0.65, 1.33, 2.32 and 3.23), number of primary branches plant-1 (21.45, 25.37, 32.17 and 39.46) and secondary branches plant-1 (64.72, 76.45, 96.56 and 109.34), plant spread (north -south) (25.32, 33.39, 40.26 and 50.38 cm), plant spread (East- west) (40.56, 55.89, 65.47 and 71.45 cm), at 30, 60, 90 and 120 DAT. The findings indicate that the integration of organic inputs of treatment, T9 (FYM @ 25 t ha⁻1 + VC @ 2.5 t ha⁻1 + Seaweed extract @ 2%) is highly effective in optimizing the growth of sweet basil.

The roles of nanoparticle-enriched biochars in improving soil enzyme activities and nutrient uptake by basil plants under arsenic toxicity

Enriched biochar with improved properties and functionality can play a significant role in providing sustainable solutions for mitigating heavy metal contamination in soil. In this experiment, the effects of solid and enriched biochars (potassium-enriched biochar (BC-K), magnesium-enriched biochar (BC-Mg), both individually and combined) were examined on soil microbial and enzyme activities, as well as nutrient uptake by basil plants cultivated in a soil with three levels of arsenic (nontoxic, 50 mg As kg−1 soil, and 100 mg As kg−1 soil). Biochar-related treatments, increased soil organic matter (65–76%), while decreased availability of arsenic (6–55%) in the soil. The microbial biomass carbon (by about 123%) and soil basal respiration (by about 256%), and soil enzymatic activities (β-glucosidase, urease, alkaline phosphatase, and dehydrogenase) were enhanced by enriched biochars under arsenic toxicity. The solid and particularly enriched biochars decreased arsenic content and improved nitrogen and phosphorus contents of roots and shoots, root length, root activity, and root and shoot biomass in basil plants. Therefore, it is conceivable to suggest that enriched biochars are superior treatments for improving nutrient absorption rates and basil growth under arsenic toxicity through decreasing arsenic mobility and increasing soil microbial activities.

Growth, phytochemical, and phytohormonal responses of basil to different light durations and intensities under constant daily light integral

Horticulture in controlled environments has been increasingly used to tackle limitations on crop production. As a crucial environmental factor, light regulate plant growth and metabolism. In the present study, basil plants were subjected to different light durations and intensities considering constant daily light integral (DLI). The lighting environment included 200, 300, and 400 µmol m− 2 s− 1 intensities for 18, 12, and 9 h, respectively. DLI amounted to 12.96 mol m− 2 d− 1 among all light treatments (LI200 for 18 h, LI300 for 12 h, and LI400 for 9 h). Half of the plants under each light treatment were exposed to 30 µmol m− 2 s− 1 of far-red light. The results indicated the general negative impact of LI400/9 on the growth of basils. Exposure to far-red light hurt the growth of the shoot, while it enhanced stem and petiole elongation. This effect was due to higher gibberellin accumulation, which resulted in shade avoidance responses. Exposure to far-red light also reduced anthocyanin and flavonoid contents, as two important nutritional components. Soluble carbohydrates increased, while storage carbohydrates decreased by increasing lighting duration/decreasing light intensity or by far-red light inclusion. The lowest antioxidant activity was detected in LI400/9. In the LI200/18, the highest level of auxin and the lowest level of cytokinin were detected, while the LI300/12 exhibited the highest level of gibberellin hormone. Low light intensity and long photoperiod enhanced plant biomass and phytochemical production and are recommended for basil production in controlled environments.

Sufficient Light Intensity Is Required for the Drought Responses in Sweet Basil (Ocimum basilicum L.)

Various environmental factors not only affect plant growth and physiological responses individually but also interact with each other. To examine the impact of light intensity on the drought responses of sweet basil, plants were subjected to maintenance of two substrate volumetric water contents (VWC) using a sensor-based automated irrigation system under two distinct light intensities. The VWC threshold was set to either a dry (0.2 m3·m−3) or sufficiently wet condition (0.6 m3·m−3) under low (170 μmol·m−2·s−1) or high light intensities (500 μmol·m−2·s−1). The growth and physiological responses of sweet basil (Ocimum basilicum L.) were observed over 21 days in the four treatment groups, where the combination of two environmental factors was analyzed. Under high light intensity, sweet basil showed lower Fv/Fm and quantum yield of PSII, compared to that under low light intensity, regardless of drought treatment. Fourteen days after drought treatment under high light intensity, stomatal conductance and the photosynthetic rate significantly reduced. Whereas plants under low light intensity showed similar stomatal conductance and photosynthetic rates regardless of drought treatment. Assessment of shoot and root dry weights revealed that plant growth decline caused by drought was more pronounced under high light intensity than under low light intensity. Thus, sweet basil showed significant declines in growth and physiological responses owing to drought only under high light intensity; no significant changes were observed under low light intensity.

Effects of Micronutrients on the Growth and Phytochemical Composition of Basil (Ocimum basilicum L.) in the Field and Greenhouse (Hydroponics and Soil Culture).

The current research was conducted to compare the growth, yield, and phytochemical composition of basil (Ocimum basilicum) in the open field and the soil and hydroponic cultivation in a greenhouse. Furthermore, the effect of foliar spraying of micronutrients on this crop was also evaluated. In each of the cultivation systems, foliar spraying of one micronutrient, either iron sulfate (Fe), zinc sulfate (Zn), copper sulfate (Cu), manganese sulfate (Mn), or boric acid (B), at a concentration of 0.1% was applied in a randomized complete block design. Plants grown in the hydroponic system had higher yield and biomass. The concentration of the elements K, Ca, Mg, N, P, Mn, Fe, B, and Zn in the leaves of hydroponic plants was higher. Contrarily, plants cultivated in the field showed higher stem dry weight, essential oil content, phenolic and flavonoid content, and antioxidant activity. The level of methyl chavicol was higher in the hydroponic culture, but the level of 1,8-cineole was much lower in this cropping system. Foliar spraying of Cu, Mn, Zn, Fe, or B significantly increased leaf dry weight and anthocyanin content. In field conditions, the highest levels of phenolics, flavonoids, and antioxidant capacity were observed with Zn or Mn application. In the hydroponic system, foliar spraying of Zn or B led to the highest antioxidant capacity, and total phenolic and flavonoid contents. Overall, the basil plants cultivated in the field showed higher bioactive ingredients. However, the essential oil of plants cultivated in the hydroponic system had a higher economic value due to its higher percentage of methyl chavicol.

Effect of integrated nutrient management practices on growth, oil yield and economics of sweet basil (Ocimum basilicum L.)

Effect of integrated nutrient management practices on growth, oil yield and economics of sweet basil (Ocimum basilicum L.)

Optimizing Peat and Wood Fiber Blends: Impacts of Liming and Fertilization on Growth of Petunia (Petunia x hybrida Vilm.) and Basil (Ocimum basilicum L.)

This study investigated the effects of substrates composed of various ratios of wood fiber and peat (0, 25, 50, 75, and 100% peat (v/v)) mixed with different amounts of lime (0, 2, 4, 6, and 8 g L−1) and start fertilizer (0, 2, and 4 g L−1 Multimix) on the growth and biomass accumulation of petunia (Petunia x hybrida Vilm ‘Finity F1 Purple’) and basil (Ocimum basilicum L. ‘Marian’) in an ebb-and-flow greenhouse system. Growth parameters included plant height, weight, canopy diameter, and chlorosis symptoms for petunia, along with substrate pH and EC measurements. Petunia showed optimal growth in substrates with higher peat content, while basil produced satisfactory biomass across a pH range of 5–7 regardless of substrate type. Optimal petunia cultivation in 100% wood fiber required a significant dose of start fertilizer without lime. Monitoring pH and EC using pour-through and press methods revealed a pH decrease in substrates with added start fertilizer, while substrates with higher wood fiber content were less acidic. Substrates with over 50% (v/v) wood fiber without lime showed a rapid pH increase over five weeks. The pour-through method generally underestimated EC values compared to the press method. These findings contribute to optimizing the wood fiber/peat blends for sustainable horticulture.

Anthroponics: Application and effects on growth of parsley, rhipsalis, coriander, and basil fed with urine fertiliser

Urine has emerged as a promising nutrient-rich waste stream suitable for plant fertigation. Biological nitrification in a membrane bioreactor offers an effective method for producing liquid fertiliser from urine, extracting essential nutrients into a final product. Despite the increasing interest in hydroponics and urban horticulture, research exploring the efficacy of urine-derived fertilisers remains scarce. This study employed three growing systems, including nutrient film technique open channels in outdoor setup, pot-drench under glasshouse conditions, and deep-water culture (DWC) under laboratory conditions to investigate the efficiency of urine-derived fertiliser compared to commercial fertilisers. The results demonstrated comparable growth responses in parsley, rhipsalis, coriander, and basil, as demonstrated by the analysis of several parameters including biomass, stem numbers and length, and nutritional composition in shoots and roots. In conclusion, urine-derived fertilisers showed promising potential as a sustainable alternative to synthetic fertilisers, laying the foundation for the development of a circular economy of nutrients in agriculture.

Aquaponic growth of basil (Ocimum basilicum) with African catfish (Clarias gariepinus) in standard substrate combined with a Humicacid Fiber-Substrate (HFS)

A major challenge in agriculture, horticulture and aquaponics practices is the reduction of mineral fertilisers and peat to reduce CO2 emissions and increase sustainability. This study used a three-phase-natural fertiliser, the Humicacid Fiber-Substrate (HFS), made from natural regenerative organic and mineral-fractions (Humus-Mineral-Complex), to reduce the peat content in plant pots for aquaponics farming. Basil (Ocimum basilicum) growth was compared with i) 100% standard media substrate ("Einheitserde", white peat 80%, clay 20%), and ii) 85% "Einheitserde" and 15% of HFS under irrigation with aquaculture process waters from an extensive and intensive production of African catfish (Clarias gariepinus) under coupled aquaponic conditions. The substitution with 15% HFS and use of intensive fish water resulted in comparable plant growth to a fertiliser solution as control, and in higher leaf width and leaf green weight and lower root dry weight compared with the standard media substrate "Einheitserde". Basil leaf chlorophyll content from the aquaponics was higher compared with local market plants. This suggests the possible substitution of the peat substrate "Einheitserde" with at least 15% HFS to reduce the natural peat fraction. Further studies on crop-specific substrates are needed to reduce peat in aquaponics farming plant cultivation.

Vermicompost Effects on Soil Chemistry and Biology: Correlations with Basil's (Ocimum basilicum L.) Total Phenolic Content and Phenological Traits

This study explores the effects of vermicompost on the chemical and biological properties of soils, their nutrient content, and the impact on the growth and phenolic content of basil (Ocimum basilicum L.). Using a controlled experimental setup, we tested five dosages of vermicompost (0%, 4%, 12%, 20%, and 24%) to evaluate their influence on soil biological activity by measuring basal respiration (CO2-C), microbial biomass C (MBC-C), and dehydrogenase enzyme activity (DHA) as well as on basil's growth parameters and total phenolic content (TPC). The results show that vermicompost addition to soil enhanced soil microbial activity in direct proportion to the dose of vermicompost. The application of lower dosages of vermicompost (4% and 12%) significantly enhanced both fresh and dry weights, suggesting an improvement in nutrient uptake and soil structure, which likely facilitated better root growth and nutrient absorption. However, higher dosages (20% and 24%) were associated with reduced growth metrics, likely due to nutrient overload or salt stress. Notably, the highest vermicompost concentration (24%) led to a substantial increase in total phenolic content (TPC) in basil leaves, correlating with decreased growth metrics. This response indicates the plant's defensive mechanism against oxidative stress caused by excess nutrients or salinity from the vermicompost. A multiple regression analysis following a correlation analysis also revealed an inversely proportional relationship between phosphorus content in the soil and total phenolic content in basil leaves. Our findings illustrate that while moderate vermicompost dosages optimize plant growth and health, higher concentrations can strategically enhance phenolic content due to nutrient overload or salt-induced stress. These results offer critical insights for tailoring organic amendment applications to balance plant growth and biochemical properties in agricultural practices.

Application of combined fertilizers to improve growth, yield and essential oil composition of basil (<i>Ocimum basilicum</i> L.)

Application of combined fertilizers to improve growth, yield and essential oil composition of basil (<i>Ocimum basilicum</i> L.)

Optimisation of indole acetic acid production by Neopestalotiopsis aotearoa endophyte isolated from Thymus vulgaris and its impact on seed germination of Ocimum basilicum

BackgroundMicrobial growth during plant tissue culture is a common problem that causes significant losses in the plant micro-propagation system. Most of these endophytic microbes have the ability to propagate through horizontal and vertical transmission. On the one hand, these microbes provide a rich source of several beneficial metabolites.ResultsThe present study reports on the isolation of fungal species from different in vitro medicinal plants (i.e., Breynia disticha major, Breynia disticha, Duranta plumieri, Thymus vulgaris, Salvia officinalis, Rosmarinus officinalis, and Ocimum basilicum l) cultures. These species were tested for their indole acetic acid (IAA) production capability. The most effective species for IAA production was that isolated from Thymus vulgaris plant (11.16 µg/mL) followed by that isolated from sweet basil plant (8.78 µg/mL). On screening for maximum IAA productivity, medium, “MOS + tryptophan” was chosen that gave 18.02 μg/mL. The macroscopic, microscopic examination and the 18S rRNA sequence analysis indicated that the isolate that given code T4 was identified as Neopestalotiopsis aotearoa (T4). The production of IAA by N. aotearoa was statistically modeled using the Box-Behnken design and optimized for maximum level, reaching 63.13 µg/mL. Also, IAA extract was administered to sweet basil seeds in vitro to determine its effect on plant growth traits. All concentrations of IAA extract boosted germination parameters as compared to controls, and 100 ppm of IAA extract exhibited a significant growth promotion effect for all seed germination measurements.ConclusionsThe IAA produced from N. aotearoa (T4) demonstrated an essential role in the enhancement of sweet basil (Ocimum basilicum) growth, suggesting that it can be employed to promote the plant development while lowering the deleterious effect of using synthetic compounds in the environment.

Biochar-based nanoparticles mitigated arsenic toxicity and improved physiological performance of basil via enhancing cation exchange capacity and ferric chelate reductase activity

The modified biochars have positive effects in reducing heavy metal toxicity for plants. However, the mechanism and extent of these effects on mitigating arsenic toxicity and plant performance are not clear. Thus, a pot experiment was conducted as factorial to evaluate the potential of fresh and enriched biochars with potassium and magnesium nano-sulfates [potassium-enriched biochar (K-BC), magnesium-enriched biochar (Mg-BC) in individual and combined forms] on reducing arsenic toxicity (non-contamination, 50, and 100 mg NaAsO2 kg−1 soil) in basil plants. Biochar-related treatments reduced plant arsenic absorption rate (up to 24%), arsenic content of root (up to 38%) and shoot (up to 21%) and root tonoplast H+-ATPase activity (up to 30%). The fresh and particularly enriched biochars improved soil properties (pH, CEC, and available iron content), ferric chelate reductase activity, iron, potassium and magnesium contents of plant tissues, chlorophyll content index, photochemical efficiency of photosystem II, relative electron transport rate, leaf area, and basil growth (shoot and root dry weight). These results revealed that enriched biochars are useful soil amendments for improving physiological performance of plants via reducing heavy metal toxicity and enhancing cation exchange capacity, nutrient availability and ferric chelate reductase activity. Therefore, soil amendment by enriched biochars could be a sustainable solution for enhancing plant productivity in contaminated soils via mitigating environmental impacts. This is an environmentally friendly method for using the natural wastes to overcome the adverse effects of soil pollutants on medicinal plants.

Enhancing french basil growth through synergistic Foliar treatment with copper nanoparticles and Spirulina sp.

BackgroundThis study investigates a novel idea about the foliar application of nanoparticles as nanofertilizer combined with a natural stimulant, blue-green algae Spirulina platensis L. extract, as a bio-fertilizer to achieve safety from using nanoparticles for enhancement of the growth and production of the plant. Thus, this experiment aimed to chemically synthesize copper nanoparticles via copper sulfate in addition to evaluate the impact of CuNPs at 500, 1000, and 1500 mg/L and the combination of CuNPs with or without microalgae extract at 0.5, 1, and 1.5 g/L on the morphological parameters, photosynthetic pigments accumulation, essential oil production, and antioxidant activity of French basil.ResultsThe results revealed that foliar application of CuNPs and its interaction with spirulina extract significantly increased growth and yield compared with control, the treatments of 1000 and 1500 mg/L had less impact than 500 mg/L CuNPs. Plants treated with 500 mg/L CuNPs and 1.5 g/L spirulina extract showed the best growth and oil production, as well as the highest accumulation of chlorophylls and carotenoids. The application of CuNPs nanofertilizer caused a significant increase in the antioxidant activity of the French basil plant, but the combination of CuNPs with spirulina extract caused a decrease in antioxidant activity.ConculosionTherefore, foliar application of natural bio-fertilizer with CuNPsis necessary for obtaining the best growth and highest oil production from the French basil plant with the least damage to the plant and the environment.

Effect of Seed Storage Duration and Methods on Growth and Yield of Basil Variety

The experiment was conducted at Wondogenet Agricultural Research Center to determine the effect of seed storage period and methods on the growth and yield of basil. Basil seeds stored in three storage methods (Plastic jar, Polypropylene, and Cotton bags) for six storage periods (initial period, one, three, six, nine, and twelve months) were used to evaluate the impact of the treatments on the yield and performance in the field. The field experiment was arranged by using RCBD design with three replications. The data were taken at the 90% physiological maturity stage. The results showed a significant difference between the treatments. The results indicated that the interaction effects of basil seed storage time and methods had a significant (P<0.05) effect on Primary Branch Number, Plant Height, Umbrella length, Dry, and Fresh biomass. The umbrella number of the storage methods had an accelerating trend with raising storage duration to 3 months storage, after that it shows a declining trend with raising storage time. The two storage methods plastic jar and bag maintained the seed in good condition for 3 months further, storage of the seed leads to the shortest plant and umbrella length. The highest dry biomass was recorded at 3 months stored seed in a plastic bag which was not significantly different from 3 months stored seed in a plastic jar. Basil dry biomass for the jar stored seed ranged from 0.85 to 3.33, 0.91 to 3.42 ton/ha for plastic bag whereas for the cotton bag, it ranged from 0.6 to 2.26 ton/ha. In general, seeds stored for three months had shown better growth and yield.

Morpho-physiological traces of exogenous biogenic iron oxide nanoparticles in basil seedlings

Chemical fertilizers used in plant development and differentiation have become a global problem affecting the entire ecosystem, especially soil pollution. Food production demand with the increasing population has encouraged scientists to use biogenic nanoparticles in the agricultural field. Evaluation of growth, development, and differentiation processes of sweet basil (Ocimum basilicum L.) seedlings at gradually increasing concentrations of biogenic iron oxide nanoparticles (BIO-NPs) were identified by morphological and physiological parameters in this study. The results showed that growth parameters reached the maximum value at 100 mg/L but were less at other concentrations. At similar concentration, the stomatal density of the leaf was the maximum, while the stomatal area showed the lowest value. The levels of H2O2 and malondialdehyde (MDA) decreased in the treated seedlings. BIO-NPs increased the antioxidant defense and supported its growth by changing the antioxidant enzyme activities, H2O2, and MDA contents. The BIO-NP treatment provided positive improvements in phytochemical content in parallel with the growth and development of sweet basil seedlings. Different growth parameters, physiological results, supporting enzyme activities, and biochemical data revealed the contribution of the BIO-NP treatments to the growth and development of sweet basil seedlings. BIO-NPs improved higher phytochemical production of sweet basil, which may be suitable for its propagation on a commercial scale.

Comparative Analysis of Growth Parameters in Hydroponic and Soil-Grown Systems of Ocimum basilicum L. (Basil)

This study’s primary objective was to compare basil growth (Ocimum basilicum L.) in three different systems: soil-based, EBB & Flow, and Nutrient Film technique (NFT). During the 45-day growth period, the following factors were examined: plant height, root length, leaf length, leaf breadth, stem diameter, number of leaves, water quality parameters, soil macro- and micronutrients, and nutrient uptake. The findings showed that, for NFT, EBB hydroponics, and soil-based systems, the plant heights of basil plants at the end of the growth period were 40 ± 3.10, 30.80 ± 2.82, and 28.50 ± 3.17 cm, respectively. The NFT hydroponics system yielded the maximum root height measurement of 34 ± 3.92cm for basil plants. After the trial, the amount of nutrients absorbed from hydroponically grown water ranged from 70 to 87%, indicating the phytoremediation of wastewater. According to the findings, the NFT hydroponic system significantly outperformed the EBB and soil-based methods for growing basil plants regarding growth characteristics. The study’s findings indicate that the hydroponic system is the most effective technique for growing basil.

Effects of mulching and nutritional strategies on the growth and yield of sweet basil (Ocimum basilicum L.)

The effect of mulching and nutrition on the growth and yield of sweet basil was examined by experimenting with four mulch treatments, viz., without mulch, black plastic, silver plastic, and organic mulch combined with four different nutrient compositions, viz., a 100% recommended dose of fertilizer (RDF), a combination of 75% RDF with humic acid, 75% RDF with microbial consortia and 75% RDF, and humic acid and microbial consortia, which served as sixteen treatment combinations with 100% RDF without mulch as a control. Among the different treatments, mulch had a significant effect on growth and yield parameters, with black plastic mulch having the maximum plant height (88.20 cm), number of branches (14.95), plant spread (5415 cm2), stem diameter (12.64 mm), fresh herbage yield (24.55 t/ha) and dry herbage yield (8.72 t/ha). Thus, the study revealed that among the various mulch treatments, black plastic mulch significantly enhanced the growth and yield parameters of sweet basil, demonstrating its efficacy in promoting optimal plant development and productivity.

Promotive Effect of Non-Woven Polylactide/Natural Rubber Composites on Growth and Biochemical Constituents of Purple Basil (Ocimum basilicum L.)

Presently, modern trends focused on eco-friendly “green” technologies are increasing the widespread use of biodegradable polymers and polymer composites in agricultural production. In this work, non-woven materials, polylactide/natural rubber (PLA/NR) composites with a different natural rubber content, were used as substrates for growing purple basil (Ocimum basilicum L.) in the multisoil compound in a phytochamber. It was shown that non-woven PLA/NR fabrics stimulate the growth and development of purple basil plants during the growing season. Compared to the control sample, the germination and biometric indicators of basil were higher when using PLA/NR substrates. The production of basil’s photosynthetic pigments also increased. While using PLA/NR fabrics with a rubber content of 10 and 15 wt.%, the number of chlorophyll a was enhanced by 1.8–2.2 times and chlorophyll b by 2.5–3.2 times. In the process of the hydrolytic and enzymatic degradation of the polymer matrix, organic compounds are formed that provide additional nutrition for basil plants. Non-woven PLA/NR composites became brittle after the experiment. The PLA/NR morphology, structure, and rheological properties changed, which indicates the course of biodegradation processes in the polymer matrix.

Effect of Biochar, Potting Mixture and their Blends to Improve Ocimum basilicum Growth in Sandy Soil

Biochar has received significant interest as a soil amendment but its relative performance to addition of high-quality soil is not well assessed. This study compared different loadings of cabbage-waste biochar produced at 360 °C against potting mixture (PM) as amendments to enhance basil (Ocimum basilicum) growth in sandy soil. Pot tests for six conditions of biochar, PM or their blend were tested at both 2% or 6% loading, plus a control, with soil and plant characteristics monitored. The combination of 6% biochar and 6% PM increased seed germination by 85% compared to the control, while 6% biochar applied in sandy soil reduced seed germination by 29%. However, significant benefits were realized later in the growing stage in comparison to the control and most other tested conditions. The 6% application of biochar to the soil enhanced leaf chlorophyll and carotenoid contents, membrane stability index, and leaf relative water content by 198%, 150%, 15%, and 5% over the control, respectively. The highest shoot and total biomass were achieved with the combined application of 6% biochar and PM, followed by 6% biochar only. When comparing only application of biochar or PM, biochar was the more favorable soil amendment at both 2% and 6% loadings. Biochar was also effective at enhancing plant uptake of beneficial nutrients, while immobilizing Al, which is detrimental, in the soil. Overall, biochar is a better soil amendment for sandy soil than equivalent quantities of PM, but mild benefits occur through their combined application.