AbstractWe present a novel multiproxy data set (bulk and clumped isotopes on gastropod shells and variations in ostracod assemblages) of the Hampshire Basin (Southern‐England) that sheds light on the connection between the North Sea and the Atlantic Ocean through the English Channel during the deposition of the Barton clay formation (latest Lutetian—Bartonian, middle Eocene; ∼41–40 Ma). During this time period, the English Channel operated as a gateway between the warmer Atlantic Ocean and the colder North Sea waters. High‐latitude water mixing combined with the regional hydrological cycle and sea‐level variations, may have contributed to mitigate the water temperatures in the Hampshire Basin, with an average recorded of ca. 25°C. In the uppermost part of the section the connection between the two water masses was limited or absent as evidenced by warmer (up to ca. 35°C) Atlantic Ocean temperatures in the Hampshire Basin. The large differences in average oxygen isotope composition of seawater (δ18Owater) recorded (from δ18Owater −1.7‰ to δ18Owater of −0.7‰) evidenced large salinity differences between the Atlantic Ocean and the North Sea. Ostracods suggest that the temporary connection between those two water masses was caused by relative regional sea‐level variations. This scenario could be partially linked to glacio‐eustatic sea‐level changes related to climate fluctuations probably associated with the MECO event.