Tidal mixing in a shelf sea south of Japan (Bungo Channel) plays an important role in modulating the water exchange between the Seto Inland Sea and Pacific Ocean. In this study, based on moored observations and model results from the Japan Coastal Ocean Predictability Experiment—Tides (JCOPE-T), the generation, propagation, and dissipation of semidiurnal internal tides in the Bungo Channel are investigated. Observational results indicate that semidiurnal internal tides induce strong baroclinic currents reaching 0.3 m/s. Their energy shows obvious spring-neap tidal cycles, generally coinciding with the local barotropic tidal forcing. By conducting the empirical orthogonal function analysis, we find that the observed semidiurnal internal tides are mainly dominated by the first two baroclinic modes. The JCOPE-T results suggest two main generation sites for semidiurnal internal tides in the region: one is located at a narrow strait north of the Bungo Channel, while the other is at the shelf break south of the Bungo Channel. The latter makes a major contribution to the observed semidiurnal internal tides. Northward internal tides generated at the shelf break are superposed with those generated at the narrow strait, causing a complex interference pattern in the channel. The temporal variation of semidiurnal internal tides in the Bungo Channel is affected by several factors. The intraseasonal variation of semidiurnal internal tides can be modulated by the Kuroshio warm water intrusion (Kyucho) because the occurrence of Kyucho changes the stratification in the channel and hence affects the energy conversion. The seasonal variation of semidiurnal internal tides in the Bungo Channel is determined mainly by the seasonally varying stratification; while those generated at the shelf break are under the combined influence of seasonal stratification and background currents. Southward internal tides from the shelf break are refracted due to the spatially varying stratification and background currents. The varying Kuroshio path and strength modulate the refraction of internal tides.