Barley Grain Research Articles

The effect of mini-till and no-till technologies on productivity and quality of malting barley grain

The purpose of the current work was to study the effect of mini-till, no-till and traditional agricultural technologies on productivity and quality of malting barley grain. Field trials were carried out in three crop rotation sequences ‘winter rye – spring wheat – barley’ on medium loamy gray forest soil of Chuvashia with 2.42 % of humus; 188 mg/kg of mobile phosphorus; 160 mg/kg of exchangeable potassium and 6.2 of exchangeable acidity. During the vegetation period of 2020, the hydrothermal coefficient was 1.04; in 2021 it was 0.60; and in 2022 it was 1.05. The object of the study was an early-maturing variety ‘Elf’. Traditional agricultural technology was based on autumn disk plowing and autumn plowing, early spring harrowing, seedbed cultivation and sowing with subsequent rolling. Mini-till cultivation included autumn disk plowing, spring cultivation and sowing with the multifunctional complex “Cultibar 9000”. No-till cultivation consisted of autumn treatment with the non-selective herbicide “Sprut Extra" and “direct” spring sowing “Cultibar 9000”. The seeding rate was 200 kg/ha. The dose of mineral fertilizers was N30P20К20. The crops were protected from weeds, diseases, and pests. On average, the largest (2.49 t/ha) productivity of malting barley grain was obtained using mini-till technology, and the smallest (2.34 t/ha) when using no-till. The mean productivity with traditional agricultural technology was lower than the maximum by 0.03 t/ha, but larger than the minimum by 0.12 t/ha. The analysis of the qualitative characteristics has shown a reliable effect of agricultural technologies on the brewing properties of barley grain. Thus, traditional technology contributed to obtaining grain of the 1st class, mini-till technology contributed to obtaining grain of the 2nd class, and no-till technology contributed to obtaining grain of the 3rd class of the interstate standard.

Barley Grain Proteome Assessment Using Multi-Environment Trial Data and Machine Learning.

Proteomics can be used to assess individual protein abundances, which could reflect genotypic and environmental effects and potentially predict grain/malt quality. In this study, 79 barley grain samples (genotype-location-year combinations) from Californian multi-environment trials (2017-2022) were assessed using liquid chromatography-mass spectrometry. In total, 3104 proteins were identified across all of the samples. Location, genotype, and year explained 26.7, 17.1, and 14.3% of the variance in the relative abundance of individual proteins, respectively. Sixteen proteins with storage, DNA/RNA binding, or enzymatic functions were significantly higher/lower in abundance (compared to the overall mean) in the Yolo 3 and Imperial Valley locations, Butta 12 and LCS Odyssey genotypes, and the 2017-18 and 2021-22 years. Individual protein abundances were reasonably predictive (RMSECV = 1.25-2.04%) for total, alcohol-soluble, and malt protein content and malt fine extract. This study illustrates the role of the environment in the barley proteome and the utility of proteomics and machine learning to predict grain/malt quality.

Agroecological estimation of the effect of crop rotation and mineral fertilizers on productivity and quality of spring barley grain in the Central Blackearth region of Russia

The purpose of the current study was to determine the effect of crop rotations and doses of mineral fertilizers on productivity, grain quality of spring barley and fertility of typical chernozem in the Central Сhernozem Region (CBR). The study was conducted in a multifactorial trial located on the watershed plateau of the research and production department No. 2 of the Federal Agricultural Kursk Research Center in 2018 and 2022. The soil of the experimental plot was typical medium-loamy chernozem, with 5.9 % of humus. There have been studied such factors as grain-fallow-row and grain-grass-row crop rotations; with fertilizer doses and no fertilizers, N30Р30К30, N60Р60К60. The highest biological productivity of spring barley up to 5.99 t/ha was identified in the grain-fallow-row crop rotation with the dose of N60Р60К60. However, the presence of perennial leguminous grasses in the grain-grass-row crop rotation contributed to a smaller decrease in humus, the maximum loss of which in it was 0.14 %, which was 0.02 % lower than in the grain-fallow-row crop rotation. The trends of agroecological effect of crop rotations and mineral fertilizers on the indicators of spring barley grain production were estimated with a digital method, namely the Harrington desirability function. There has been established an “acceptable, satisfactory” effect of crop rotations and mineral fertilizers on the studied indicators (di = 0.50), but in relation to individual indicators of grain production, the level of effect of crop rotations varies significantly from di = 0.12 “completely unacceptable” to di = 0.83 “most acceptable”. The ecological properties of crop rotation are manifested in the estimation of its effect on the humus content in the soil: “acceptable, satisfactory” (di = 0.38–0.52). The comprehensive estimation of the effect of mineral fertilizers for most indicators has increased proportionally to their dose, reaching di = 0.49–0.65, which indicates their “acceptable, satisfactory” effect.

Structural variation in the pangenome of wild and domesticated barley.

Pangenomes are collections of annotated genome sequences of multiple individuals of a species1. The structural variants uncovered by these datasets are a major asset to genetic analysis in crop plants2. Here we report a pangenome of barley comprising long-read sequence assemblies of 76 wild and domesticated genomes and short-read sequence data of 1,315 genotypes. An expanded catalogue of sequence variation in the crop includes structurally complex loci that are rich in gene copy number variation. To demonstrate the utility of the pangenome, we focus on four loci involved in disease resistance, plant architecture, nutrient release and trichome development. Novel allelic variation at a powdery mildew resistance locus and population-specific copy number gains in a regulator of vegetative branching were found. Expansion of a family of starch-cleaving enzymes in elite malting barleys was linked to shifts in enzymatic activity in micro-malting trials. Deletion of an enhancer motif is likely to change the developmental trajectory of the hairy appendages on barley grains. Our findings indicate that allelic diversity at structurally complex loci may have helped crop plantsto adapt to new selective regimes in agricultural ecosystems.

Influence of mineral fertilizers and liming on the dynamics of CO<sub>2</sub> emissions, crop yield and quality of crop products of crop rotation link

In the field experiment was investigated the effect of repeated lime application on CO2 emissions from the soil, yield and quality of plant products against the background of mineral fertilizers N90P90K90, on dark gray forest heavy loamy soil. As a result of liming and improvement of the acid regime in the soil, prerequisites are created to enhance the vital activity of beneficial microorganisms in the experiment. It was revealed that an increase in biological activity (carbon dioxide emissions) against the background of the application of mineral fertilizers N90P90K90 and CaCO3 ensures high yield and product quality, contributes to favorable conditions for preserving soil fertility and to a greater extent stimulates the development of microorganisms using mineral forms of nitrate nitrogen. With the systematic use of mineral fertilizers N90P90K90 at the stage of crop rotation, an increase in the quality of cultivated crops was noted (the nutritional value of the green mass of clover; the content of crude protein, protein, gluten, starch in barley grain; the nature of grain in winter wheat). In different years of research, according to weather conditions, grain with a high content of crude gluten, protein, nature in winter wheat grain, starch in barley, ash nutrition elements in clover was obtained. The best option turned out to be using dolomite flour and (N90P90K90). All the considered quality indicators of winter wheat improved under the influence of fertilizers and lime, and the difference with the control was: protein – 0,64–1,78%; crude gluten – 1,6–4,3%; grain nature – 11–26 g/l. The highest productivity was obtained when applying mineral fertilizers and lime: barley – 3,53 t/ha, clover hay of the first year of use – 77,53 t/ha, winter wheat – 7,89 t/ha, compared with the control without the use of fertilizers.

Metarhizium anisopliae seed priming alleviates drought-induced oxidative stress and improves growth of barley (Hordeum vulgare L.)

Increasing crop resilience to drought stress through microorganisms is a sustainable approach. This study evaluated the efficacy of the endophytic fungus Metarhizium anisopliae MetA1 (MA) for improving drought tolerance of barley by analyzing various morphological, physiological, biochemical, and yield factors. Barley grains were treated with MA (1 × 108 spore/ml) and a pot experiment was conducted with three high-yielding barley genotypes: BARI Barley-10, BARI Barley-6, and BARI Barley-9 under three drought conditions: no drought (100% field capacity, FC), moderate drought (50% FC), and severe drought (25% FC). Under drought conditions, MA priming significantly enhanced shoot and root biomass, leaf characteristics, photosynthetic pigment content, and activities of various antioxidant enzymes including superoxide dismutase, catalase, glutathione S-transferase, ascorbate peroxidase, peroxidase, glutathione peroxidase, glutathione reductase, dehydroascorbate reductase, monodehydroascorbate reductase, glyoxalase-I, and glyoxalase-II in all barley genotypes. Furthermore, there was an observed increase in the levels of non-enzymatic antioxidants such as ascorbate and glutathione. MA treatment also led to a significant reduction in stress markers like methylglyoxal, malondialdehyde, lipoxygenase, hydrogen peroxide, and calcium influx along with an increase in proline and potassium content in barley leaves in stressed conditions. Crop growth and yield related attributes in barley were improved, which is evidence of better physiological and biochemical changes under both stress and non-stressed conditions. As such, the study provides evidence suggesting that MA-mediated seed priming is an effective strategy for improving drought tolerance not only in barley but also possibly other crops.

Protein aggregation behavior during highland barley dough formation induced by different hordein/glutelin ratio

To investigate why highland barley dough cannot form a gluten structure, the mechanism by which the ratio of hordein/glutelin affects protein aggregation behavior during the formation of barley dough was investigated. It was observed that the pasting properties of the reconstituted flour were markedly diminished (1154–1303 × 10−3 Pa•s). The 50Hordein-50Glutelin sample exhibited the highest farinograph quality number. The K, A22 (48.82–65.26 %), hardness, free -SH (0.76 %–0.95 %), random coil, and hydrogen bonding (0.091–0.179 g/100 g) exhibited a notable increase with a reduction in the hordein/glutelin ratio. Conversely, β-sheet (27.39 %–40.11 %) and fluorescence intensity declined pronouncedly. The 50H-50G facilitated the reinforcement of protein crosslinking and polymerization, enhanced the continuity and order of the protein network structure, and further augmented the dough's viscoelasticity. The results would regulate the macroscopic quality of barley and other miscellaneous grain products through endogenous components, establishing a foundation for enhancing their quality.

Determination and Quantification of Folpet in Barley using HPLC-DAD Analysis

Although rоutinе pesticide analysеs are mostly performed using GC-MS, GC-MS/MS and/or LC-MS/MS, some pesticides especially those with relatively high MRLs and good detector response could also be precisely quantified with less sensitive equipment which gives the opportunity to some laboratories to expand their scope of work. The study focuses on HPLC-DAD method development for detection of folpet in barley grain. QuEChERS method was performed for extraction after which, additional clean-up was performed using PSA and polypropylene filtration. Pesticide separation was achieved using mobile phase of acidified CAN (pH 2.5) and water. The initial mobile phase was 60:40% (v/v), the elution gradient starts from 80:20% (v/v) to 100:0% (v/v) in 7 min and holds 100:0% (v/v) up to 8.5 min at a flow rate of 1 ml/min after which in the next 6.5 min the column was re-equilibrated to 40% phase-B. Calibration was performed using matrix-matched calibration standards. The obtained limits of detection (LOD) and quantification (LOQ) were 0.18 and 0.55 mg/kg, respectively, and the linear regression coefficient was 0.9973. Recovery, repeatability and reproducibility were investigated at three fortification levels (0.1 mg/kg, 0.6 mg/kg, and 1.6 mg/kg) and were found acceptable with relative standard deviation less than 10%. The method was applied for the analysis of three barley grain samples obtained from different producers and showed that folpet was present in one sample below the LOQ. Overall, the developed method is suitable for the determination of folpet in barley grains at levels below the established MRL of 1 mg/kg.

Relationships Between Photosynthetic Efficiency and Grain Antioxidant Content of Barley Genotypes Under Increasing Nitrogen Rates

Nitrogen fertilization may affect the functioning of photosynthesis as well as the chemical composition and antioxidant potential of cereal grains. Little is known about the relationship between the efficiency of photosynthesis and the content of phenolic compounds in barley grain, especially in conditions of varying nitrogen availability. In this regard, a field experiment was conducted to examine the responses of two primary barley genotypes with elevated phenolic compound content (TPC) in grain and an intensive modern cultivar H. v. vulgare with high protein content to increasing nitrogen fertilization (rates of 0, 30, 60 and 90 kg N ha−1) during the study years, which differed in terms of hydrothermal conditions. The leaf greenness index (SPAD) and chlorophyll fluorescence parameters were evaluated on three occasions throughout the growing season. Following the harvest, the chemical composition of the grains, including phenolic acids, flavonoids and antioxidant potential, was evaluated. The antioxidant potential and chemical composition of the grain, including TPC and protein content, depended to the greatest extent on genetic and environmental factors, and only then on nitrogen fertilization. Nitrogen increased the TPC content and antioxidant capacity ABTS+ of the grains of all studied genotypes and the protein content in H. v. vulgare grain. Rates of 60 and 90 kg N ha−1 resulted in a significant increase in the SPAD, PIabs and Fv/Fm in BBCH 34 and 57. A positive correlation was confirmed between the SPAD and PIabs and the content of TPC and ABTS+ in the grain. The dependence of qualitative characteristics on the Fv/Fm was also demonstrated. The primary genotypes are characterized by a greater genetic potential for the synthesis of phenolic compounds than the modern cultivar H. v. vulgare. The synthesis of phenolic compounds, and thus their accumulation in the grain, is clearly stimulated by unfavorable environmental factors and moderate nitrogen rates and depends on the chlorophyll content in the leaves and the efficiency of photosynthesis. N fertilization has a beneficial effect on the content of phenolic compounds in grain resulting from the improvement in the SPAD and PIabs. The chemical composition of grain and the increase in antioxidant potential are determined by the Fv/Fm, which is low under hydrothermal stress conditions.

Impact of Harvest Delay and Barley Variety on Grain Nutritional Composition and Mycotoxin Contamination

This study investigated the effects of delayed harvesting, varying meteorological conditions, and barley variety on Fusarium spp. infection rates, nutritional composition, and mycotoxin contamination in barley grains. Field experiments were conducted from 2020 to 2022 and involved two barley varieties: ‘Laureate’ for malting and ‘Luokė’ for feed. The results indicated that the dominant Fusarium species isolated were F. avenaceum, F. culmorum, F. poae, F. sporotrichioides, F. tricinctum, and F. equiseti. These tended to increase in number with delayed harvest times and were more prevalent during harvest periods of higher precipitation (p < 0.05). Malting barley had higher starch and lower protein content compared to feed barley (p < 0.05). Delayed harvesting generally increased dry matter, crude fat, and crude ash contents while decreasing crude protein, zinc, and iron contents (p < 0.05). Mycotoxin analysis revealed significant differences under specific weather conditions. HT-2 toxin levels were higher under slightly warmer and wetter conditions during flowering, with harvest conditions similar to the long-term average. Zearalenone levels increased with dry, warm growing seasons followed by rainy harvests. Nivalenol and enniatin levels increased with rainy growing seasons and dry, warm harvests. Deoxynivalenol concentrations did not reach the limit of quantification throughout the study. No consistent trend was observed for higher contamination in any specific barley variety (p > 0.05). The strongest correlations between mycotoxins and nutritional value indicators were observed with less-studied mycotoxins, such as nivalenol and enniatins, which exhibited negative correlations with crude protein (p < 0.01), crude fat (p < 0.05), and zinc (p < 0.01), and positive correlations with crude ash (p < 0.05) and phosphorus (p < 0.01).

Restoration of the spike architectonics in ancient barley excavated at the twelfth-century settlement of Usvyaty

Background. The data are presented on the architectonics of ancient barley spikes from the 12th century, excavated in 2019 at Usvyaty Settlement. Modern molecular genetics approaches were used to study domestication genes (Btr1, Btr2, and Vrs) in ancient and contemporary barleys (germplasm accessions preserved at VIR).Materials and methods. The carbonized kernels found by archaeologists during the excavations at Usvyaty were analyzed. Primers for domestication genes were designed, and PCR was performed on contemporary and ancient barley grains. Ancient kernels were studied in accordance with the rules established for organizing a paleogenetics laboratory, which excluded any contamination with contemporary DNA. Fragments of domestication genes from contemporary and ancient barley grain samples underwent Sanger sequencing. Results. Ancient DNA was isolated and enriched. The analysis of domestication gene sequences made it possible to reconstruct the ancient barley spike’s features. Conclusion. The ancient cereal crop architectonics was restored to ascertain a brittle two-row spike of ancient barley

Effect of Replacing Barley Grain by Urea in the Diet of Iraqi Lambs

This study conducted to seeks the effects of partially changing barley grain (BG) with different levels amount of urea on growth performance, rumen fermentation, nutrient digestion and blood parameters in fattening lambs. Twelve Awassi male lambs were divided according to body weight (BW) into three equal groups (4 lambs per each group) and housed in individual pens and assigned to one of three dietary treatments in a randomized block design: barley grain (BG)were given to animals according to the body weight of lambs (3 % of BW) and (BG) reduced and replace with 0, 10, 20, gm urea. Compared with the control the lambs fed the reduced GB diet plus urea was higher (p < 0.01) concentrations of ruminal ammonia and increased with increasing the urea supplementation. As well as increased the crude protein (CP) intake , blood urea nitrogen (BUN), final BW, dry matter intake (DMI) and average daily gain (ADG) were observed with the increasing urea addition to the diet, results showed that 10 gm of urea could substitute of 10 gm BG per kg feed DM without any negative effect on health performance of fattening lambs when fed urea.

Evaluation of spring barley cultivars released in Belarus under the environmental conditions of the Northern Trans-Urals

Background. Studying the genetic diversity of ex situ barley accessions under contrasting environmental conditions (humid with a lack of heat, favorable, arid, etc.) makes it possible to assess them according to the duration of the growing season, resistance to lodging, potential productivity, drought resistance, grain quality, and environmental plasticity. Materials and methods. The results of an 8-year trial (2016–2023) of 11 spring barley accessions of Belarusian origin in the northern forest-steppe zone of Tyumen Province are presented. The experiments followed the protocols adopted for the state variety trials. An SKS-6-10 seeder was used for sowing, the plot area was 5 m2 , and cv. ʻAbalakʼ served as the reference. The seeding rate was 550 viable seeds per 1 m2 . Results. A majority of the studied accessions matured later than the medium-ripening reference cultivar. Weather conditions during the growing season in the Northern Trans-Urals had a decisive effect on the harvest formation, protein content and starch content in barley grain (74.6%, 70.8% and 81.8%, respectively). The effect of the genotype on these indicators was 7.9%, 17.5% and 6.2%, respectively. Starch content also depended on the genotype × environment interaction (9.7%). Fat content was almost equally affected by the genotype (25.1%) and the environment (26.7%). The genotype × environment interaction was more significant (31.2%). Conclusion. The following sources were identified among the tested cultivars: ‘Vodar’ (high yield, drought tolerance, etc.); ‘Magutny’ (high yield, and environmental plasticity); ‘Khago’ (large grain, low hull content, and increased protein and starch content levels); ‘Lipen’ (high test weight, increased starch content, and environmental plasticity); ‘Fobos’ (drought tolerance, and increased protein content); ‘Dublet’ (lodging resistance, and increased protein content); ‘Taler’ (reduced protein content, and environmental plasticity); ‘Pospekh’ (reduced protein content).

Beta-Glucans Improve the Mammary Innate Immune Response to Endotoxin Challenge in Dairy Ewes.

This study evaluated short-term immune responses of dairy ewes supplemented with barley β-glucan (BG) following an intramammary Escherichia coli lipopolysaccharide (LPS) challenge. In the adaptation period, 36 ewes were fed an alfalfa hay diet ad libitum and barley grain cv. Hispanic (3.8% BG). Then, ewes were assigned into three experimental groups: (1) Control (CON), the same previous diet (13.3 g BG/d); (2) high β-glucans barley (HBG), new barley (cv. Annapurna) containing 10% BG (35 g BG/d); (3) intraperitoneally injected (INP) with a 1.4% BG solution dose (2 g BG/ewe). At d 9, all ewes were infused with an E. coli LPS or saline solution in each udder half. After the challenge, rectal temperature (RT), milk yield and composition, somatic cell count (SCC), and plasma interleukins (IL-1α and IL-1β) were monitored daily. The INP treatment revealed a transitory increase in RT and decreased milk yield by 38%. Milk fat, protein, and SCC increased in LPS-treated udders but not by BG treatment. The IL-1α plasma concentration was similar among groups but INP ewes showed a lower IL-1β concentration suggesting a lower inflammatory response. The BG administration appears more effective intraperitoneally than orally, which needs additional study.

The effect of nitrogen fertilisation, biostimulant application and extra-root phosphorus fertilization on yield and quality of malting barley grain

A series of small-plot field trials were conducted between 2019 and 2021 with the spring barley variety Francin. The impact of Vital Root and YaraVita KOMBIPHOS on the grain quality of malting barley at varying nitrogen fertilization rates (50 kg·ha-1 N, 70 kg·ha-1 N, 90 kg·ha-1 N, and 110 kg·ha-1 N) was investigated. The following parameters were evaluated: grain size, thousand grain weight, specific weight, starch content, and protein content. The portion of grain <2.5 mm, weight of thousand grains, and specific weight decreased with increasing nitrogen fertilization. Additionally, a decrease in starch content was observed with an increase in protein content in the grain. The application of Vital Root and YaraVita KOMBIPHOS at lower nitrogen fertilization rates up to 70 kg·ha-1 N had a positive effect on grain quality.

Protective and Detoxifying Effects of Resveratrol on Zearalenone-Mediated Toxicity: A Review

Zearalenone (ZEA) is a mycotoxin produced by Fusarium spp. fungi and is widely found in moldy corn, wheat, barley, and other grains. ZEA is distributed to the whole body via blood circulation after metabolic transformation in animals. Through oxidative stress, immunosuppression, apoptosis, autophagy, and mitochondrial dysfunction, ZEA leads to hepatitis, neurodegenerative diseases, cancer, abortion, and stillbirth in female animals, and decreased sperm motility in male animals. In recent years, due to the influence of climate, storage facilities, and other factors, the problem of ZEA pollution in global food crops has become particularly prominent, resulting in serious problems for the animal husbandry and feed industries, and threatening human health. Resveratrol (RSV) is a natural product with therapeutic activities such as anti-inflammatory, antioxidant, and anticancer properties. RSV can alleviate ZEA-induced toxic effects by targeting signaling pathways such as NF-κB, Nrf2/Keap1, and PI3K/AKT/mTOR via attenuating oxidative damage, inflammatory response, and apoptosis, and regulating cellular autophagy. Therefore, this paper provides a review of the protective effect of RSV against ZEA-induced toxicity and its molecular mechanism, and discusses the safety and potential clinical applications of RSV in the search for natural mycotoxin detoxification agents.

MRI-Seed-Wizard: Combining Deep Learning Algorithms with Magnetic Resonance Imaging Enables Advanced Seed Phenotyping.

Evaluation of relevant seed traits is an essential part of most plant breeding and biotechnology programs. There is need for non-destructive, three-dimensional assessment of the morphometry, composition, and internal features of seeds. Here, we introduced a novel tool, MRI-Seed-Wizard, which integrates deep learning algorithms with non-invasive magnetic resonance imaging (MRI) for its use in the new domain - plant MRI. The tool enabled in vivo quantification of 23 grain traits, including volumetric parameters of inner seed structure. Several of these features cannot be assessed using conventional techniques, including X-ray computed tomography. MRI-Seed-Wizard was designed to automate the manual processes of identifying, labeling, and analyzing digital MRI data. We further provide advanced MRI protocols that allow the evaluation of multiple seeds simultaneously to increase throughput. The versatility of MRI-Seed-Wizard in seed phenotyping was demonstrated for wheat (Triticum aestivum) and barley (Hordeum vulgare) grains, and is applicable to a wide range of crop seeds. Thus, artificial intelligence, combined with the most versatile imaging modality - MRI, opens up new perspectives in seed phenotyping and crop improvement.

The effect of increasing canola oil and soybean oil addition to beef cattle rations based on corn silage and barley grain on the in vitro ruminal gas production and rumen fermentation

AbstractThe purpose of the current experiment was to determine the effects of ruminal fermentation parameters of the additions of canola or soybean oils at different rates (4, 8 and 12%) to beef cattle total mix rations (TMR), based on corn silage and barley grain. The addition of 4% soybean oil to TMR positively affected in vitro gas production, net energy lactation (NEL), metabolic energy (ME), and organic matter digestion (OMd). The additions of 8% and 12% of soybean oil to TMR linearly decreased ME, NEL and OMd (p < 0.05). The additions of 4, 8 and 12% canola oil to the TMR linearly decreased the in vitro gas production and estimated fermentation values (ME, NEL and OMd) (p < 0.05). All doses of soybean or canola oils in TMR reduced the molarities of total short‐chain fatty acids (tSFCA), acetic (AA), butyric (BA), propionic (PA), valeric (VA), iso‐valeric (IVA) and iso‐butyric acids (IBA) for in vitro fermentation fluid (p < 0.05). There was a negative correlation between the increasing dietary stearic, oleic and linoleic acid and the end‐products of in vitro rumen fermentation. However, increasing dietary α‐linolenic acids had no adverse effect on in vitro ruminal fermentation end‐products. As a result, 4% addition of the soybean oil, which included a higher rate of α‐linolenic acid and saturated fatty acids and a lower rate of oleic, linoleic acids according to those of canola oil, to the TMR positively affected in vitro ruminal fermentation. In addition, the ≥8% addition of canola or soybean oil adversely affected the in vitro fermentation values.

Effect of feeding two types of concentrates in morning and evening meals and two types of fat supplement on diurnal patterns of plasma parameters in lactation dairy cows

The diurnal patterns of feed intake and TMR composition influence blood parameters in dairy cows; however, the effect of feeding TMR with different compositions in the morning and evening meals is not well characterized. In a completely random design, forty Holstein cows (110 ± 30 days postpartum) were randomly assigned to four treatment groups, with 10 cows per treatment. The treatments were as follows: a base diet containing 50 % cereal starch from barley grain and 50 % cereal starch from corn grain in the morning and evening meal + either prilled fatty acids supplement (EqP) or calcium salts of fatty acids supplement (EqCS); a base diet containing 25 % cereal starch from barley grain, 75 % cereal starch from corn grain in the morning meal, and 75 % cereal starch from barley grain and 25 % cereal starch from corn grain in the evening meal + either prilled fatty acids supplement (DiP) or calcium salts of fatty acids supplement (DiCS). Dry matter intake was affected by treatments (P ˂ 0.01). The highest intake was observed for EqP, EqCS, DiP,and DiCS. 3.5 % fat-corrected milk and milk fat percentage was significantly higher for EqP than other treatments (P ˂ 0.01), but other milk components were not significantly different among treatments (P ˃ 0.05). A significant difference was observed for glucose, cholesterol, TG, AST, and insulin concentration among treatments (P ˂ 0.01). The concentrations of all plasma parameters (glucose, cholesterol, TG, BUN, AST, insulin, and NEFA) significantly changed over 24-h period (P ˂ 0.01). Plasma concentrations of glucose, cholesterol, TG, BUN, AST, insulin, and NEFA displayed a treatment-by-time interaction (P ˂ 0.01). In general, changes in the cereal starch ratio (corn and barley) in TMR in each meal during a day affect the 24-h concentration of plasma parameters, but there was no strong evidence of leverage for cows’ metabolism.

Значение предшественников и минерального питания в формировании урожайности и качества ярового ячменя в Пермском крае

Abstract. The purpose is to evaluate the influence of previous culture and mineral nutrition on the yield and quality of grain and seeds of spring barley in the conditions of the Middle Urals. Methods. The research was conducted at the Perm Agricultural Research Institute (a branch of the Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences) over the period 2021–2023 on sod-podzolic heavy loam soil. A two-factor field experiment with different previous culture and mineral nutrition backgrounds was carried out. Data were processed using B. A. Dospekhov’s methods, and grain quality was determined using standard techniques. Results. The limiting factor affecting plant growth and productivity is soil moisture, which influences both the size and quality of crops. Studies have shown that the soil under vetch-oat mixtures had the highest level of productive moisture at the start of the growing season. However, by the end of the season, the highest level was found under clover. During the research period, the most significant decrease in soil moisture was observed after barley, possibly due to the smaller surface area covered by this crop. The study of barley seed germination showed that they met the required standards. Seeds grown after vetch-oat had the best germination rate (95 %), while those preceding barley had the worst (92 %). This suggests that choosing the right precursor and providing optimal nutrition can lead to increased yield and improved grain quality. The yield of barley can vary widely, ranging from 1.06 to 3.77 tons per hectare. The study results show that using vetch-oat as a precursor can provide the best indicators for barley yield and quality. The optimal combination of precursor and mineral nutrient background can significantly improve the efficiency of barley production in agriculture. Scientific novelty: The complex effect of fertilizers and precursor substances on the formation of barley productivity and quality is being studied in the Perm region for the first time. The methods of barley management and quality control studied allow for obtaining highly productive seeds and achieving the planned yield.