The aim of the current in vivo, observational study was to investigate the effects of different hoof manipulations on landing duration (LandD), location (ICloc) and angle of initial contact (ICangle) in the front feet of horses. A novel, hoof-mounted, inertial measurement unit sensor system (IMU) was used. Ten sound, crossbred horses were fitted with an IMU sensor at the dorsal hoof wall and examined barefoot and after trimming. Additionally, the application of 120 g lateral weights and 5° medial side wedges as well as steel, aluminium, egg bar and lateral extension shoes were tested. Horses were guided in a straight line on firm ground. The use of steel shoes increased LandD compared to barefoot and enhanced the individual ICloc in trot. Application of rolled toe shoes caused a longer LandD than use of plain shoes. None of the other modifications significantly influenced the timing or spatial variables of hoof landing. Trimming and shoeing have less impact on the landing pattern of horses than assumed in practice. Still, the use of steel shoes changes sliding properties of the hooves on firm ground and increases weight causing a longer LandD and reinforcement of the individual ICloc.