In seedling technology, Banana seedlings are undergoing a profound transformation with the integration of advanced innovation, referred to as tissue culture banana seedling. The study delves into the science of seedling technology and innovation adoption within the banana farming sector. We explore the dynamic interactions between tissue culture innovation and farmer adoption. The study adopted a positivism philosophy, cross-sectional survey design, and purposive sampling techniques. A sample size of 350 banana farmers in Kilimanjaro was drawn from the population of the study using the Yamen formula for sample size determination. Since the outcome variable was dichotomous the binary logistic regression model was employed. The result indicates that the highest mean was for the statement banana tissue culture seedling grow faster compared to local sucker (M=3.75), indicating the speed of growth is seen as a clear observable benefit. Standard deviations were relatively low, ranging from 0.826 to 0.872, indicating less variation in responses. These results suggest smallholder farmers perceive tissue culture banana seedlings as having moderately high observability, especially related to the visible benefits of faster planting growth and income generation compared to using local banana seedlings. We shed light on the adoption of tissue culture banana seedlings. Adoption is reshaping traditional seedling suckers practices, enhancing productivity and business opportunity. Farmers' adoption will lead to economic development and job creation. Farmers markets provide opportunities for small farmers and businesses to sell their products, and support to meet the growing demand for locally produced food.