Antibiotic resistance is a global health crisis. Roughly two-thirds of all antibiotics used are in production animals, which has the potential to impact on the development of antibiotic resistance in bacterial pathogens of humans. There is little visibility on the extent of antibiotic resistance in the Australian food chain. This study sought to establish the incidence of antibiotic resistance among enterococci from poultry in Victoria. In 2016, poultry from a Victorian processing facility were swabbed immediately post-slaughter and cultured for Enterococcus species. All isolates recovered were speciated and tested for antibiotic susceptibility to twelve antibiotics following the Clinical Laboratory Standards Institute guidelines. Six farms and 207 birds were sampled and from these 285 isolates of Enterococcus were recovered. Eight different enterococcal species were identified: E. faecalis (n=122; 43%), E. faecium (n=92; 32%), E. durans (n=35; 12%), E. thailandicus (n=23; 8%), E. hirae (n=10; 3%) and a single each of E. avium, E. gallinarum, and E. mundtii. Reduced susceptibility to older classes of antibiotic were common, in particular: erythromycin (73%), rifampin (49%), nitrofurantoin (40%) and ciprofloxacin (39%). Two vancomycin intermediate isolates were recovered, but no resistance was detected to either linezolid or gentamicin. The relatively high numbers of a recently described species, E. thailandicus, suggests this species might be well adapted to colonise poultry. The incidence of antibiotic resistance is lower in isolates from poultry than in human medicine in Australia. These results suggest that poultry may serve as a reservoir for older antibiotic resistance genes but is not driving the emergence of antimicrobial resistance in human bacterial pathogens. This is supported by the absence of resistance to linezolid and gentamicin.