Bacterial Biofilms In Patients Research Articles

Removal of bacterial biofilm in patients undergoing fixed orthodontic treatment: a literature review

Orthodontic treatment is highly popular for restoring functional and facial esthetics in juveniles and adults. However, the prevalence of biofilm-related complications remains high. The objectives of this review are to 1) identify which of the two professional prophylaxis systems analyzed, rubber cup polishing (RCP) and air-powder polishing (APP), is the most effective in removing bacterial biofilm in patients undergoing fixed orthodontic treatment, and 2) the comfort expressed by the patient and any damage caused to the orthodontic device. The search yielded 23 scientific articles, 14 of which were excluded, and 6 met the inclusion criteria. From the studies analyzed, it is, therefore, possible to state that the APP technique is more effective than the RCP technique in removing dental plaque, and treatment is less time-consuming and safer concerning orthodontic devices, as none of the scientific articles examined did APP cause any damage to the orthodontic device or debonding of the brackets.

Prevalence of Bacterial Biofilms in Patients of Chronic Rhino Sinusitis (CRS) with or Without Nasal Polyposis

Background: Objective. There is growing evidence that bacterial biofilms may play a role in certain cases of recalcitrant CRS that do not respond to traditional medical and surgical therapies. Aim: The aim of this study was to determine the prevalence of biofilm-forming bacteria in clinical isolates of CRS patients with or without nasal polyposis. Methods: A Prospective observational study conducted in the Department of ENT & HNS GMC Srinagar and Department of Microbiology, from September 2018 to August 2020. 60 patients were enrolled in this study who reported to ENT & HNS OPD of Govt. Medical College Srinagar and diagnosed with CRS according to “clinical practice guidelines” 2015 criteria, and were willing to participate in the study. Samples were harvested in the OPD and/or intraoperatively for microscopic examination to determine biofilm presence. Statistical analysis was performed. For all statistical tests, P = .05 was considered significant. Results: In this study on 60 patients Biofilms were present in 32 (53.3%) patients whereas in 28 (46.7%) patients Biofilms were absent (includes 14 culture positive and 14 culture negative patients). Biofilm formation was detected by three different phenotypic methods. Biofilm detection rate were highest by Tissue Culture Plate (TCP) method (56.3%) followed by Tube method (TM) as (46.9%) and Congo Red Agar (CRA) method as (43.8%). Conclusion: This study further elucidates the prevalence of bacterial Biofilms in sinonasal mucosa of CRS patients as Biofilms existed in 53% of the patients under study.

Detection of catheter related blood stream infections in an ICU of a tertiary care center in Northern India

Indwelling medical devices are frequently used in all health setting with critical care units of hospitals for treatment and intervention in patient care.Microorganisms attach to surfaces resulting in the formation of a biofilm which pose a serious public health issue because of its increased resistance to antimicrobial agents and the potential to cause infections.To determine the proportion of bacterial Biofilms in patients with central venous line and to find out the organisms most commonly associated with it.The study was carried out from January 2019 to September 2020. Positive cultures were obtained from 58 of these 102 samples. The isolates were then tested for in vitro production of biofilm using a microtitre plate assay. All the isolates were subjected to antimicrobial susceptibility tests on Muller-Hinton agar by Kirby Bauer disc diffusion method based on CLSI guidelines. Demographic characteristics of the study subjects showed that among 102 catheterized patients isolated with males 58.82% and 41.1% females. Out of 58 isolates, 62.06% were gram negative bacteria (GNB) and 37.93% were gram positive. was most common isolate 27.58% followed by 20.68%, 15.5%, respectively. The total number of positive slime producers, in this study, was 23(39.6%). The highest number of strong slime producer strains was observed in case of (2 out of 5) (4 out of 12) and 3 out of 16)Antibiotic susceptibility patterns showed increased resistance towards penicillin and beta -lactum group of antibiotics, increased sensitivity to linezolid and vancomycin among gram positive organisms. Among gram negative bacteria increased resistance was seen for cephalosporins and aminigylycosides and least resistance for colistin.Colonization of indwelling medical devices with consequent biofilm production is a likely contributory factor to infections. The microorganisms survive in the hospital environment despite unfavourable conditions such as desiccation, nutrient starvation, and antimicrobial treatment. Resistance to antibiotics ladder is increasing and it’s necessary to take actions to reduce its hindrance in the future. Advanced studies in biofilm will help to prevent the more virulent factors which protect the bacteria from host immunity and to get rid of critical complications in therapy.

The discovery of bacterial biofilm in patients with muscle invasive bladder cancer.

The carcinogenic effects of microorganisms have been discovered in multiple cancer types. In urology, the development of squamous cell carcinoma of the bladder due to the parasitic infection with Schistosoma Mansoni is widely accepted. The oncogenic potential of biofilms has been studied in colorectal cancer and experimental studies have shown that bacteria such as Escherichia coli drive the development of colorectal cancer. Notably, Escherichia coli is responsible for 80% of all urinary tract infections. Recent findings suggest an altered urinary microbiome in patients with bladder cancer compared to healthy subjects. In this case series, we demonstrate our findings of biofilm formation in human bladder cancer tissue. Tissue samples from ten patients that underwent routine Transurethral Resection of Bladder Tumor (TURBT) were obtained from the Danish National Biobank. Pathological tissue was examined for presence of bacterial aggregates by Fluorescence in situ Hybridization. In two of ten patients, analysis showed abundant bacterial aggregation on the surface epithelium. Both positive cases had pT2 urothelial bladder cancer. Our findings suggest that biofilm occurs in urothelial cancer tissue indicating an association between biofilm formation and bladder cancer.

BIOFILM BACTERIA PLAYS A ROLE IN CSOM PATHOGENESIS AND HAS SIGNIFICANT CORRELATION WITH UNSAFE TYPE CSOM

Chronic suppurative otitis media (CSOM) may cause severe morbidity and mortality and remains a major health problem worldwide. The incidence of CSOM in Indonesia (1994-1996) is estimated at about 8.36 million people and CSOM general prevalence is 3.8% (Helmi 2005). This study aims to prove the existence of bacterial biofilm in patients with safe type and hazard type CSOM from mastoidectomy. The design was observational analytic with cross sectional approach. This study was conducted at the Teratai Wards, IBP Dr. Soetomo Hospital, and Electron Microscopy Unit, Faculty of Medicine, Airlangga University Surabaya, from November 2013 to June 2014. Samples of pathological tissues were taken by consecutive sampling and bacterial biofilms examination was done by SEM. SEM results categorized the biofilm bacteria as positive or negative. Biofilm bacteria tested positive when it shows three-dimensional representation of bacteria with clear shapes and sizes and clusters, the formation of amorphous material consisting of glycocalyx material surrounding the bacteria, and surface attachment. Biofilm bacteria was regarded as negative when there is no bacterial cluster, glycocalyx and surface attachment. This study was performed on 33 CSOM patients. Samples were divided into unsafe type CSOM (17 patients) and safe type CSOM (16 patients). Positive biofilm bacteria was found in 12 patients with unsafe type (75%) and 6 patients with safe type (35.35%). Negative biofilm bacteria was found in 4 patients with the unsafe type (25%) and 11 patients with safe type (67.9%). Logistic regression analysis revealed p value = 0.027, indicating the biofilm bacteria have a significant correlation with unsafe type CSOM (p <0.05). In conclusion, biofilm bacteria plays a role in CSOM pathogenesis of biofilm bacteria has significant correlation with unsafe type CSOM.

Bacterial biofilms in patients with chronic rhinosinusitis: a confocal scanning laser microscopy study.

Recent research into the pathophysiology of chronic rhinosinusitis suggests an important role for biofilms. They can be detected in both healthy and diseased nasal mucosa. Several different methods of detecting biofilms have been described. This study investigates the presence of biofilm in a larger group of patients with chronic rhinosinusitis undergoing primary functional endoscopic surgery. Sixty-one patients with chronic rhinosinusitis and 25 controls, with septal deviation, were included from 2010 to 2012. Endonasal biopsies were harvested during surgery, snap frozen in isopentane, cooled on dry ice and stored at -80oC. The samples were prepared with Invitrogens’ BacLight LiveDead kit, and investigated with confocal scanning laser microscopy for the presence of biofilm. In the chronic rhinosinusitis group 55/61 were biofilm positive as opposed to 14/25 in the control group. The difference was highly significant. The odds ratio was 7.2. Patients with chronic rhinosinusitis have a highly significant increased point prevalence of biofilms compared to controls.

Bacterial biofilms in patients with chronic rhinosinusitis: a confocal scanning laser microscopy study

Recent research into the pathophysiology of chronic rhinosinusitis suggests an important role for biofilms. They can be detected in both healthy and diseased nasal mucosa. Several different methods of detecting biofilms have been described. This study investigates the presence of biofilm in a larger group of patients with chronic rhinosinusitis undergoing primary functional endoscopic surgery. Sixty-one patients with chronic rhinosinusitis and 25 controls, with septal deviation, were included from 2010 to 2012. Endonasal biopsies were harvested during surgery, snap frozen in isopentane, cooled on dry ice and stored at -80oC. The samples were prepared with Invitrogens’ BacLight LiveDead kit, and investigated with confocal scanning laser microscopy for the presence of biofilm. In the chronic rhinosinusitis group 55/61 were biofilm positive as opposed to 14/25 in the control group. The difference was highly significant. The odds ratio was 7.2. Patients with chronic rhinosinusitis have a highly significant increased point prevalence of biofilms compared to controls.

Observation of bacterial biofilms in patients with chronic rhinosinusitis

To explore the presence of bacterial biofilms (BF) in patients with CRS and the effect of BF on clinical symptoms and postoperative outcomes. Seventy-two patients with chronic sinusitis were enrolled in this study. The control group included 15 patients with deviation of the nasal septum and 10 patients had a fracture of the nasal bone. Mucosa of the uncinate process or ethmoid near the ostium of the maxillary sinus was obtained during endoscopic sinus surgery. The specimens were subjected to scanning electron microscopy. Patients were followed for 1 year and observed by the Lund-Kennedy endoscopy, and the Haikou standard classification (ESS-1997). Statistical analysis was performed by t-test or chi-square test. Three patients were lost to follow-up. The scanning electron microscopy analysis showed bacterial biofilms in 49 of the 69 patients with chronic sinusitis. A marked destruction of the epithelium and cilia was observed in samples positive for bacterial biofilms. No bacterial biofilms were detected in the control group, and scanning electron microscopy showed normal epithelium and cilia in those specimens. There was no significant difference in gender, classification or duration of disease between the BF(-) and BF(+) groups. At six months and one year postoperative, the Lund-Kennedy endoscopy scores for CRS patients with BF (4.78 +/- 1.67; 4.55 +/- 1.61) were significantly higher than those without BF (3.65 +/- 1.39; 3.65 +/- 1.18) (t = -2.654, P < 0.01; t = -2.264, P < 0.05). Based on the Haikou standard classification, there was a significantly difference between patients with BF and those without BF (chi2 = 18.014, 22.063, P < 0.001, respectively). Different life stages of bacterial biofilms were demonstrated to be present in CRS. Gender, classification or duration of disease did not affect the presence of bacterial biofilms in patients with CRS. There is a correlation between bacterial biofilms and an unfavorable outcome in patients with CRS after ESS.

Biofilm Detection With Hematoxylin-Eosin Staining

To demonstrate that hematoxylin-eosin staining can be used to detect the presence of bacterial biofilm in patients with chronic rhinosinusitis (CRS). A prospective study. The University of Southern California University Hospital and the Department of Otolaryngology-Head and Neck Surgery, University of Southern California, Keck School of Medicine, Los Angeles. A total of 34 patients: 24 undergoing endoscopic sinus surgery for CRS and 10 undergoing septoplasty with or without turbinate reduction with no history of sinusitis, were enrolled in the study. Contiguous sections from patient samples were examined by both hematoxylin-eosin staining and fluorescence in situ hybridization (FISH) with the bacterial-specific probe EUB338 for evidence of bacterial biofilm. Biofilm was detected by hematoxylin-eosin staining in 15 of 24 patients with CRS and 1 of 10 control patients. In all cases, hematoxylin-eosin staining was found to be an accurate predictor of the presence or absence of biofilm using FISH as a control standard. Hematoxylin-eosin staining of surgical specimens is a reliable and available method for the detection of bacterial biofilm in chronic infectious disease.

The effect of endoscopic sinus surgery on bacterial biofilms in chronic rhinosinusitis

Objective To determine whether or not endoscopic sinus surgery (ESS) alters bacterial biofilms in patients with chronic rhinosinusitis (CRS) after three months of follow-up. Study Design Prospective study. Setting Department of Otorhinolaryngology–Head and Neck Surgery, University of Queensland. Subjects and Methods Participants with CRS from the Princess Alexandra Hospital Department of Otorhinolaryngology–Head and Neck Surgery and from Logan Hospital were enrolled in the study (2008-2009). The total number of patients was 28, and the age range was 18 to 65 years. All patients underwent pre- and post-ESS questionnaires, endoscopic scoring, and nasal swabs for bacterial culture. Crystal violet staining was used to assess biofilm formation on a 96-well culture plaque. Results ESS resulted in a statistically significant improvement in quality-of-life, subjective, and objective outcome measures after three months. A significant reduction was observed in biofilm density before and after ESS (2.63 versus 1.3, P = 0.043). No correlations between the reduction of bacterial biofilms with any of the subjective, objective, and quality-of-life outcomes were seen in our study. Conclusion ESS was shown to be capable of reducing the prevalence of bacterial biofilms but did not eliminate them entirely. There was no correlation between altered biofilm formation and improved outcome measures in individual patients.

Bacterial biofilms in patients with chronic rhinosinusitis

The possible presence of biofilms was examined in mucosal specimens of 15 patients, undergoing functional endoscopic sinus surgery or a modified Caldwell-Luc approach for chronic rhinosinusitis (CRS). Biofilms were found in 7 of the 15 patients, positive cultures being obtained in most samples, which supports the role of biofilms as an important factor in the pathogenesis of CRS.

Bacterial biofilms in patients with indwelling urinary catheters

Bacteria have a basic survival strategy: to colonize surfaces and grow as biofilm communities embedded in a gel-like polysaccharide matrix. The catheterized urinary tract provides ideal conditions for the development of enormous biofilm populations. Many bacterial species colonize indwelling catheters as biofilms, inducing complications in patients' care. The most troublesome complications are the crystalline biofilms that can occlude the catheter lumen and trigger episodes of pyelonephritis and septicemia. The crystalline biofilms result from infection by urease-producing bacteria, particularly Proteus mirabilis. Urease raises the urinary pH and drives the formation of calcium phosphate and magnesium phosphate crystals in the biofilm. All types of catheter are vulnerable to encrustation by these biofilms, and clinical prevention strategies are clearly needed, as bacteria growing in the biofilm mode are resistant to antibiotics. Evidence indicates that treatment of symptomatic, catheter-associated urinary tract infection is more effective if biofilm-laden catheters are changed before antibiotic treatment is initiated. Infection with P. mirabilis exposes the many faults of currently available catheters, and plenty of scope exists for improvement in both their design and production; manufacturers should take up the challenge to improve patient outcomes.

P611 Bacterial biofilms in patients with chronic rhinosinusitis

P611 Bacterial biofilms in patients with chronic rhinosinusitis

Intestinal permeability, bowel wall thickness and bacterial biofilm in patients with chronic heart failure compared to healthy controls

Effects of a Protein Optimized Diet Combined with Moderate Resistance Training on the Postoperative Course in Older Patients with Hip Fracture