Incorporating bacterial activity within concrete holds promise for enhancing its mechanical and durability characteristics. This research delves into assessing bacterial concrete's strength and adhesion behavior, investigating the synergistic effects of bacterial activity. Scientific inquiry has confirmed the effectiveness of Bacillus species as microbial agents in augmenting concrete's self repair capabilities. Bacillus Subtilis is employed for sample preparation, with a concentration of 105 cells per millilitre chosen as the parameter. The mechanical properties of bacterial concrete are analyzed, considering the impact of bacterial strains on these properties. The study also focuses on evaluating the bonding behavior of bacterial concrete through pull-out tests, aiming to assess the efficacy of bacterial activity in enhancing the bond strength of the concrete matrix. This investigation yields a comprehensive understanding of the intricate interplay of bacterial activity within concrete. The findings contribute to optimizing bacterial concentration and mix proportions for the development of sustainable and high-performance construction materials.