Background: Muscle fatigue is inevitable during resistance exercises, making its monitoring essential for maintaining athletic performance and reducing the risk of injury. Ratings of perceived exertion (RPE) and velocity loss have been reported as reliable indicators of muscle fatigue during explosive resistance exercises. However, their validity has been assessed only indirectly. This study aimed to directly examine the validity of RPE and velocity loss as markers of muscle fatigue during explosive back squat (BS) exercises. Methods: Seventeen trained men performed three BS tasks with varying volumes (low, medium, high) at 65% of their one-repetition maximum. RPE, spectral fatigue index (SFI), and velocity loss were measured throughout the tasks. Results: Significant effects were observed across conditions for overall RPE (p < 0.001) and velocity loss (p < 0.001), while no significant effect was found for SFI. RPE and SFI increased significantly as the tasks progressed (p < 0.001), while velocity did not significantly decrease. Significant but weak correlations were found between RPE and SFI (r = 0.325, p < 0.001) and between velocity loss and SFI (r = 0.224, p < 0.001). Conclusions: These findings suggest that RPE and muscle fatigue levels increase correspondingly, indicating that RPE could serve as a practical, indirect fatigue marker for explosive BS exercises. However, velocity loss may not fully reflect muscle fatigue during lower-body explosive training and should not be used as the sole indicator. Additionally, caution is warranted when applying velocity-related parameters as indirect physiological markers for resistance exercises. The significant but weak correlations between RPE, velocity loss, and SFI suggest that assessing muscle fatigue in lower-body exercises remains challenging.
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