Back Reef Research Articles

Niche breadth and divergence in sympatric cryptic coral species (Pocillopora spp.) across habitats within reefs and among algal symbionts.

While the presence of morphologically cryptic species is increasingly recognized, we still lack a useful understanding of what causes and maintains co-occurring cryptic species and its consequences for the ecology, evolution, and conservation of communities. We sampled 724 Pocillopora corals from five habitat zones (the fringing reef, back reef, and fore reef at 5, 10, and 20 m) at four sites around the island of Moorea, French Polynesia. Using validated genetic markers, we identified six sympatric species of Pocillopora, most of which cannot be reliably identified based on morphology: P. meandrina (42.9%), P. tuahiniensis (25.1%), P. verrucosa (12.2%), P. acuta (10.4%), P. grandis (7.73%), and P. cf. effusa (2.76%). For 423 colonies (58% of the genetically identified hosts), we also used psbA ncr or ITS2 markers to identify symbiont species (Symbiodiniaceae). The relative abundance of Pocillopora species differed across habitats within the reef. Sister taxa P. verrucosa and P. tuahiniensis had similar niche breadths and hosted the same specialist symbiont species (mostly Cladocopium pacificum) but the former was more common in the back reef and the latter more common deeper on the fore reef. In contrast, sister taxa P. meandrina and P. grandis had the highest niche breadths and overlaps and tended to host the same specialist symbiont species (mostly C. latusorum). Pocillopora acuta had the narrowest niche breadth and hosted the generalist, and more thermally tolerant, Durusdinium gynnii. Overall, there was a positive correlation between reef habitat niche breadth and symbiont niche breadth-Pocillopora species with a broader habitat niche also had a broader symbiont niche. Our results show how fine-scale variation within reefs plays an important role in the generation and coexistence of cryptic species. The results also have important implications for how niche differences affect community resilience, and for the success of coral restoration practices, in ways not previously appreciated.

Genomic data reveals habitat partitioning in massive Porites on Guam, Micronesia

Corals in marginal reef habitats generally exhibit less bleaching and associated mortality compared to nearby corals in more pristine reef environments. It is unclear, however, if these differences are due to environmental differences, including turbidity, or genomic differences between the coral hosts in these different environments. One particularly interesting case is in the coral genus Porites, which contains numerous morphologically similar massive Porites species inhabiting a wide range of reef habitats, from turbid river deltas and stagnant back reefs to high-energy fore reefs. Here, we generate ddRAD data for 172 Porites corals from river delta and adjacent (<0.5 km) fore reef populations on Guam to assess the extent of genetic differentiation among massive Porites corals in these two contrasting environments and throughout the island. Phylogenetic and population genomic analyses consistently identify seven different clades of massive Porites, with the two largest clades predominantly inhabiting either river deltas or fore reefs, respectively. No population structure was detected in the two largest clades, and Cladocopium was the dominant symbiont genus in all clades and environments. The perceived bleaching resilience of corals in marginal reefs may therefore be attributed to interspecific differences between morphologically similar species, in addition to potentially mediating environmental differences. Marginal reef environments may therefore not provide a suitable refuge for many reef corals in a heating world, but instead host additional cryptic coral diversity.

Bringing Back Reef Fish: Sustainable Impacts of Community-Based Restoration of Elkhorn Coral (Acropora palmata) in Vega Baja, Puerto Rico (2008–2023)

Bringing Back Reef Fish: Sustainable Impacts of Community-Based Restoration of Elkhorn Coral (Acropora palmata) in Vega Baja, Puerto Rico (2008–2023)

Microfacies and palaeoenvironments of late Cisuralian and Guadalupian (Early to Middle Permian) alatoconchid-bearing limestone in Loei fold belt, Indochina Terrane

This study presents the first record of Early Permian alatoconchid bivalves from Thailand, the Erawan section, in the north of Loei fold belt with prolific fusulines in association with gastropods, and brachiopods. Fusuline taxa are dominated by Pseudofusulina sp. with Darvasites sp., Staffella cf. labanalensis, Staffella sp., Nankinella sp., Neofusulinella sp., Schubertella sp., Pamirina darvasica, Pseudoendothyra sp. and others, indicating an Artinskian age. Fusuline wackestone with common, well-preserved smaller foraminifers, Pseudovermiporella sp. and ostracods indicates a restricted lagoon environment with low to moderate water circulation. These fossils, along with well-preserved, articulated alatoconchids in life-position, suggest the autochthonous nature of the deposits in an intertidal environment.In the central part of the fold belt, the Pak Chong section exhibits intervals of alatoconchid biostromes and coquinites. Carbonate build-ups of the alatoconchid biostromes with gregarious bivalves, in life-position embedded in a micritic wackestone matrix, suggest their preferred life habit and environment. The bivalve coquinites containing packed bivalve shells and fragments, however, indicate a high-energy event. This rock fabric along with common fusuline storm sheets suggests occasional storm events in a restricted, with moderate water circulation to open lagoon environment. Fusulines are predominated by staffellids and others including Staffella sphaerica, Staffella sp., Sphaerulina croatica, Pisolina subsphaerica, Nankinella sp., Neoschwagerina simplex, Presumatrina sp., Afghanella sp. and others, indicating a Wordian (Middle Permian) age.In the southern portion of the fold belt along the Thai-Cambodian border, the Khao Taa Ngog section contains limestone with alatoconchid shells with massive rugose corals which formed local carbonate build-ups in high-energy open platform environments. Poorly-sorted bioclasts with common fusuline tests, shell fragments and coated grains suggest an open marine, lagoon, back reef environment. The occurrence of a fusuline assemblage including Neoschwagerina sp., Yabeina sp., Lepidolina sp., Codonofusiella sp., Verbeekina verbeeki and Chusenella sp. indicates the Capitanian (late Middle Permian) age.

Diel variation of seawater volatile organic compounds, DMSP-related compounds, and microbial plankton inside and outside a tropical coral reef ecosystem

Biogenic volatile organic compounds (VOCs) play key roles in coral reef ecosystems, where, together with dimethylated sulfur compounds, they are indicators of ecosystem health and are used as defense strategies and infochemicals. Assessment and prediction of the exchange rates of VOCs between the oceans and atmosphere, with implications for atmospheric reactivity and climate, are hampered by poor knowledge of the regulating processes and their temporal variability, including diel cycles. Here, we measured the variation over 36h of the concentrations of DMSPCs (dimethylsulfoniopropionate (DMSP)-related compounds, namely DMSP, dimethylsulfoxide, acrylate, dimethylsulfide, and methanethiol as dimethyl disulfide) and VOCs (COS, CS2, isoprene, the iodomethanes CH3I and CH2ClI, and the bromomethanes CHBr3 and CH2Br2), in surface waters inside the shallow, northern coral-reef lagoon of Mo’orea (French Polynesia) and 4 km offshore, in the tropical open ocean. Comparisons with concurrent measurements of sea surface temperature, solar radiation, biogeochemical variables (nutrients, organic matter), and the abundances and taxonomic affiliations of microbial plankton were conducted with the aim to explain interconnections between DMSPCs, VOCs, and their environment across diel cycles. In open ocean waters, deeper surface mixing and low nutrient levels resulted in low phytoplankton biomass and bacterial activity. Consequently, the diel patterns of VOCs were more dependent on photochemical reactions, with daytime increases for several compounds including dissolved dimethylsulfoxide, COS, CS2, CH3I, and CH2ClI. A eukaryotic phytoplankton assemblage dominated by dinoflagellates and haptophytes provided higher cell-associated DMSP concentrations, yet the occurrence of DMSP degradation products (dimethylsulfide, dimethyl disulfide) was limited by photochemical loss. Conversely, in the shallow back reef lagoon the proximity of seafloor sediments, corals and abundant seaweeds resulted in higher nutrient levels, more freshly-produced organic matter, higher bacterial activity, and larger algal populations of Mamiellales, diatoms and Cryptomonadales. Consequently, DMSP and dimethylsulfoxide concentrations were lower but those of most VOCs were higher. A combination of photobiological and photochemical processes yielded sunny-daytime increases and nighttime decreases of dimethylsulfide, dimethyl disulfide, COS, isoprene, iodomethanes and bromomethanes. Our results illustrate the important role of solar radiation in DMSPC and VOC cycling, and are relevant for the design of sampling strategies that seek representative and comparable measurements of these compounds.

Sedimentology Study Of Exposed Formations In Al-Assad Valley, Al-Baghdadi Area, Western Iraq

The presence of the Sheikh Alas Formation, the Shurau Formation, and the Euphrates Formation in the area of the current study. The inference of the Sheikh Alas Formation was from Nummlite fossils and planktonic Foraminifera fossils. The Euphrates Formation was deposited in a shallow marine environment, the Sheikh Alas Formation was deposited in an environment in for reef, and the Shurau Formation was deposited in an environment Back reef. The environment of the Euphrates Formation is a shallow lagoon environment for the presence of milliolied fossils, which represent the Miocene age as evidenced by the presence of the Borlis melo melo fossil. As for the environment of the Sheikh Alas Formation, it was deposited in a marine environment in for reef due to the presence of Planktonic foraminifera fossils and the Nummlite fossil, which represents the Oligocene age, and the environment of the Shurau Formation In a shallow, lagoon marine environment Back reef, there are abundant milliolid fossils. The boundary between the Miocene formations and the Oligocene formations is characterized by an unconformable boundary composed of a layer of basal conglomerate with a thickness of 6.5 m, which is affiliated with the Euphrates Formation in terms of age.

Effects of year‐long exposure to elevated pCO2 on the metabolism of back reef and fore reef communities

AbstractThe implications of ocean acidification are acute for calcifying organisms, notably tropical reef corals, for which accretion generally is depressed and dissolution enhanced at reduced seawater pH. We describe year‐long experiments in which back reef and fore reef (17‐m depth) communities from Moorea, French Polynesia, were incubated outdoors under pCO2 regimes reflecting endpoints of representative concentration pathways (RCPs) expected by the end the century. Incubations were completed in three to four flumes (5.0 × 0.3 m, 500 L) in which seawater was refreshed and circulated at 0.1 m s−1, and the response of the communities was evaluated monthly by measurements of net community calcification (NCC) and net community productivity (NCP). For both communities, NCC (but not NCP) was affected by treatments and time, with NCC declining with increasing pCO2, and for the fore reef, becoming negative (i.e., dissolution was occurring) at the highest pCO2 (1067–1433 μatm, RCP8.5). There was scant evidence of community adjustment to reduce the negative effects of ocean acidification, and inhibition of NCC intensified in the back reef as the abundance of massive Porites spp. declined. These results highlight the risks of dissolution under ocean acidification for coral reefs and suggest these effects will be most acute in fore reef habitats. Without signs of amelioration of the negative effects of ocean acidification during year‐long experiments, it is reasonable to expect that the future of coral reefs in acidic seas can be predicted from their current known susceptibility to ocean acidification.

Subenvironments of Jeribe Formation (M. Miocene) deduced from microfacies study, Zurbatiya Area, South-East of Iraq

The current study of Jeribe Formation (Middle Miocene) at Zurbatiya area, South-East of Iraq. It is, structurally, located in the Low Folded Zone. The topic depends mainly on the study of microfacies under a microscope. One surface section in the Zurbatiya area is represented in the Wadi Al-Numur section. It is composed basically of dolomitic limestone. Petrography provides a diversity of fauna, such as benthic foraminifera Miliolid, Borelis melo curdica, and Rotalia in addition to Ostracoda, Algae, Coral, and Mollusks. Borelis melo curdica is considered an index fossil for the Jeribe Formation. The non-skeletal grains like peloids, ooids intraclasts, and extraclasts. The Jeribe Formation was affected by many diagenetic processes like neomorphisim, dissolution, cementation, dolomitization, compaction, and silicification. Sedimentary microfacies are classified into three main microfacies, which are dolomudstone, dolowackestone, and dolopackstone. These microfacies were divided into many submicrofacies, which are peloidal dolomudstone, ostrocods dolomudstone, rotaliids dolowackstone, miliolid dolowackstone, bioclasts dolowackstone, green and red algae dolowackstone, red algae dolopackestone, and coral dolopackestone. The evidence from petrography and microfacies analysis supports that the Jeribe Formation was deposited in the back reef, reef, and lagoon environments.

Facies Analysis and Sequence Stratigraphy of Late Cretaceous Aqra Formation, Northern Iraq

Aqra Formation is a shallow marine deposit of the late – Cretaceous sedimentary cycle. According to the field lithological description, the two studied sections of the Aqra Formation are subdivided into three units. Based on sedimentological characteristics and fossils assemblage eleven facies have been recognized in both studied sections, their alternated association points to a wide range of depositional environments extended from tidal flat, back reef, reef, to fore- reef. Based on the fossil's appearance, the studied sections comprised two fossils biozone {Orbitoides media zone (part) and Liftusia morgani zone}, it is age determined to be late Campanian – Early Maastrictian in both studied sections. Sequence stratigraphic analysis, as calibrated by sedimentary facies and biostratigraphy delineated one sequence (Aqra sequence 1) of 2nd order, and their MFI correlation result with MFS studies in the Arabian platform indicated to maximum flooding stage dominated on the Arabian plate at the Latest Campanian.

Pocillopora tuahiniensis: a new species of scleractinian coral (Scleractinia, Pocilloporidae) from French Polynesia.

Pocillopora tuahiniensis sp. nov. is described based on mitochondrial and nuclear genomic data, algal symbiont genetic data, geographic isolation, and its distribution pattern within reefs that is distinct from other sympatric Pocillopora species (Johnston et al. 2022a, b). Mitochondrial and nuclear genomic data reveal that P. tuahiniensis sp. nov. is a unique species, sister to P. verrucosa, and in a clade different from that of P. meandrina (Johnston et al. 2022a). However, the gross in situ colony appearance of P. tuahiniensis sp. nov. cannot easily be differentiated from that of P. verrucosa or P. meandrina at Moorea. By sequencing the mtORF region, P. tuahiniensis sp. nov. can be easily distinguished from other Pocillopora species. Pocillopora tuahiniensis sp. nov. has so far been sampled in French Polynesia, Ducie Island, and Rapa Nui (Armstrong et al. 2023; Edmunds et al. 2016; Forsman et al. 2013; Glin et al. 2017; Mayfield et al. 2015; Oury et al. 2021; Voolstra et al. 2023). On the fore reefs of Moorea, P. tuahiniensis sp. nov. is very abundant 10 m and is one of the most common Pocillopora species at these depths (Johnston et al. 2022b). It can also be found at a much lower abundance at shallow depths on the fore reef and back reef lagoon. The holotype is deposited at the Smithsonian Institution as USNM-SI 1522390 and the mtORF Genbank accession number is OP418359.

Analysis of the Bottom Topography of the Reservoir Due to Sediment Trapping (According to the Krasnodar Reservoir, Russia)

Morphometric descriptions of reservoirs are usually limited to the type, shape, altitude position, bed size and volume of water in them. The article presents the results of the analysis of the bottom topography of the Krasnodar reservoir and the transformations of this for 2005-2021. The analysis was carried out based on the materials of bathymetric surveys for the usable volume of the reservoir on an area of 224 km2 with the creation of digital elevation models. The topography of the reservoir bottom is represented by flat sections of flooded accumulative plain with prevailing slopes of about 0.2–0.4°, dissected by riverbeds of lower-order tributaries. The transformation of the topography is caused by gradual silting. The total volume of sediments for this area in 2005-2021 amounted to 127 million m3 with an average siltation layer of 0.4 m. To describe the morphological properties of the bottom topography, we used geomorphometry techniques with the calculation of the BPI index (Bathymetric Position Index) and the classification of mesoscale topography forms based on it. For the riverbed, there are topography forms related to three types of surfaces: flat (Lower Bank Shelves), concave (Depressions, Deep Depressions) and convex (Reef Crests, Back Reefs, Mid-Slope Ridges). The constructed maps reflect the differentiated morphology of the bed surface, the evolution of topography forms and the change in roughness under conditions of continuous transformation of the basin and allow judging the prevailing morphogenetic processes. Morphologically, the coastal zone and the shallow part of the riverbed are the most difficult to construct. Here, along with long-shore reef crests of different genesis, deep depressions and simple depressions in the form of underwater channels on the deltas of extension can form on the accumulative shoal.

Fine-scale geographic risk assessment of oxybenzone sunscreen pollution within Hanauma Bay using hydrodynamic characterization and modeling

Hanauma Bay's coral reef system is threatened by sunscreen pollution. Understanding the hydrodynamic nature of the bay is crucial for understanding the transport and fate of pollutants within the bay. This study conducted a comprehensive hydrodynamic analysis, revealing significant aspects of current patterns and their influence on sunscreen pollutant behavior. The analysis demonstrated the formation of flows that drive currents parallel to the shoreline, resulting in increased pollutant retention time over sensitive reef areas. Direct flushing currents were identified as playing a role in reducing pollution buildup. Particle dynamics analysis highlighted the importance of considering temporal dynamics and their implications for pollutant pathways, particularly through the swash zone during high tide phases. The study identified primary current patterns near the reef area and emphasized the circular behavior within the water body, affecting corals' susceptibility to bleaching in the southwestern part of Hanauma bay. To understand where oxybenzone concentrations were a threat to wildlife, we created a geographic model that integrated ecological risk assessment with hydrodynamic behavior in a given system, which we designate the Risk Quotient Plume – the geographic area where the concentration is above the threat level for a chemical. The study found high oxybenzone concentrations throughout the bay, threatening coral, fish, and algae populations. Oxybenzone's distribution indicated a serious threat to the entire back reef habitat and a hinderance to coral restoration efforts. The study also emphasizes the need to consider the hydrodynamic behavior of pollutants and their interaction with microplastics in the bay. Overall, the findings provide insights into hydrodynamics and pollutant dispersion in Hanauma Bay, supporting effective pollution management and conservation strategies.

Corallivory on small Porites colonies increases with coral colony size but is reduced by macroalgal associational refuge

Numerous tropical macroalgae provide associational refuge to other benthic organisms, presumably due to their physical structure and/or production of chemical metabolites. One feature determining their effectiveness as an associational refuge is likely to be the size of the organism benefitting from the refuge. Using a manipulative experiment in the back reef of Moorea, French Polynesia, we tested if the macroalga Turbinaria ornata provided an associational refuge from fish corallivores for small colonies of massive Porites spp., and how this differed with colony size (20–100 mm diameter). Tissue loss through corallivory increased with colony size but was ~ 72% less for Porites colonies associated with T. ornata versus colonies separated from this macroalga, while dense macroalgae beds on contemporary reefs negatively impact the recruitment, growth and survival of corals, small colonies of Porites appear to benefit, through reduced corallivory, by associating with the macroalga Turbinaria. This association may come at a cost (e.g., reduced growth) and should be the focus of future research.

Analysis of marine diversity and anthropogenic pressures on Seixas coral reef ecosystem (northeastern Brazil)

Coral reefs, vital and ecologically significant ecosystems, are among the most jeopardized marine environments in the Atlantic Ocean, particularly along the northeastern coast of Brazil. The persistent lack of effective management and conservation has led to fragmented information on reef use and pressures, hindering the understanding of these ecosystems' health. Major difficulties and challenges include inadequate data, diverse anthropogenic pressures, and the complex interaction between marine species. This study sought to bridge this knowledge gap by conducting a comprehensive analysis of marine diversity and anthropogenic pressures, specifically focusing on Seixas coral reef near João Pessoa city, an area notably impacted by tourism. Utilizing 25 monitoring transects, subdivided into 1 m2 quadrants, the marine diversity was meticulously evaluated through innovative procedures including (a) sedimentological and geochemical field surveys, (b) application of Shannon-Weaver diversity and Simpson dominance indices, (c) cluster analysis, (d) species identification of macroalgae, coral, and fish, and (e) an examination of anthropogenic interactions and pressures on the coral reef. The assessment encompassed three distinct zones: Back Reef, Reef Top, and Fore Reef, and identified a total of 25 species across 15 genera and 10 fish families. The findings revealed the prevalence of brown macroalgae, fish, and coral, with heightened abundance of red macroalgae in the Fore Reef, which also exhibited the greatest diversity (2.816) and dominance (0.894). Original achievements include the identification of specific spatial variations, recognition of the anthropogenic factors leading to ecological changes, and the formulation of evidence-based recommendations. The study concludes that escalating urbanization and burgeoning daily tourist visits to the reef have exacerbated negative impacts on Seixas coral reef's marine ecosystem. These insights underscore the urgent need for strategic planning and resource management to safeguard the reef's biodiversity and ecological integrity.

Recruitment of Psostlarval Spiny Lobster, Stone Crabs, and Larval Fish to Back-Reef Nursery Habitats in the Florida Keys, USA

Back reef areas contain a mosaic of relatively shallow habitats that serve as a key nursery for reef species. Expansive back reef habitats in the lower Florida Keys, USA border the Atlantic Ocean and the Gulf of Mexico. A combination of floating, artificial settlement substrates and plankton nets moored in channels connecting coastal waters and back reef habitats quantified spiny lobster (Panulirus argus) and stone crab (Menippe sp.) settlement, and larval fish ingress, respectively, during 1999 and 2002–2003. The key finding was that channels connecting coastal waters with back reef areas serve as conduits for ingressing larval spiny lobster, stone crabs, and fishes. The results highlight the important role of Atlantic Ocean sources of spiny lobster pueruli and lutjanid fish larvae in this back reef system, and Gulf sources of stone crabs. Spiny lobster settlement generally peaked in August and September and occurred on the Atlantic Ocean side of this back reef system; settlement of stone crabs peaked at the same time as spiny lobster yet occurred predominantly on the Gulf side. Recruitment of larval fish was higher on the Atlantic than the Gulf side, with 37% of fish species collected nearly exclusively on the Atlantic side and about 25% collected nearly exclusively on the Gulf side. Channel habitats appear critical to supporting the nursery role of back reef habitats and should receive special consideration in future ecosystem studies, as well as during spatial planning and evaluations of essential fish habitat.

Bio-physical determinants of sediment accumulation on an offshore coral reef: A snapshot study

Sediments are found on all coral reefs around the globe. However, the amount of sediment in different reservoirs, and the rates at which sediments move between reservoirs, can shape the biological functioning of coral reefs. Unfortunately, relatively few studies have examined reef sediment dynamics, and associated bio-physical drivers, simultaneously over matching spatial and temporal scales. This has led to a partial understanding of how sediments and living reef systems are connected, especially on clear-water offshore reefs. To address this problem, four sediment reservoirs/sedimentary processes and three bio-physical drivers were quantified across seven different reef habitats/depths at Lizard Island, an exposed mid-shelf reef on the Great Barrier Reef. Even in this clear-water reef location a substantial load of suspended sediment passed over the reef; a load theoretically capable of replacing the entire standing stock of on-reef turf sediments in just 8 h. However, quantification of actual sediment deposition suggested that just 2 % of this passing sediment settled on the reef. The data also revealed marked spatial incongruence in sediment deposition (sediment trap data) and accumulation (TurfPod data) across the reef profile, with the flat and back reef emerging as key areas of both deposition and accumulation. By contrast, the shallow windward reef crest was an area of deposition but had a limited capacity for sediment accumulation. These cross-reef patterns related to wave energy and reef geomorphology, with low sediment accumulation on the ecologically important reef crest aligning with substantial wave energy. These findings reveal a disconnect between patterns of sediment deposition and accumulation on the benthos, with the ‘post-settlement’ fate of sediments dependent on local hydrodynamic conditions. From an ecological perspective, the data suggests key contextual constraints (wave energy and reef geomorphology) may predispose some reefs or reef areas to high-load turf sediment regimes.

Trypanites ichnofacies and its multifarious significance: A case study from the Miocene Boka Bil Formation of Manipur, India

Bioerosional structures are well preserved in the Miocene Boka Bil Formation, exposed in the Kaiphundai section, Thangjing Hill Range of Manipur, India. Eleven ichnospecies representing the Trypanites Ichnofacies, viz., Entobia cateniformis, E. ovula, Gastrochaenolites anauchen, G. cluniformis, G. lapidicus, G. orbicularis, G. ornatus, G. torpedo, Maeandropolydora sulcans, Trypanites solitarius and T. weisei, are recorded from the two sections of the Boka Bil Formation. Two distinctive ichnoassemblages, Entobia-dominated and Gastrochaenolites-dominated are recognised. The Entobia-dominated assemblage suggests relatively deeper marine conditions in the deeper part of the back reef or fore reef while the Gastrochaenolites-dominated assemblage points shallower marine conditions, underwater depth of a few metres in the back reef to patch-reef zone of a back-reef lagoon.

Viruses of a key coral symbiont exhibit temperature-driven productivity across a reefscape

Viruses can affect coral health by infecting their symbiotic dinoflagellate partners (Symbiodiniaceae). Yet, viral dynamics in coral colonies exposed to environmental stress have not been studied at the reef scale, particularly within individual viral lineages. We sequenced the viral major capsid protein (mcp) gene of positive-sense single-stranded RNA viruses known to infect symbiotic dinoflagellates (‘dinoRNAVs’) to analyze their dynamics in the reef-building coral, Porites lobata. We repeatedly sampled 54 colonies harboring Cladocopium C15 dinoflagellates, across three environmentally distinct reef zones (fringing reef, back reef, and forereef) around the island of Moorea, French Polynesia over a 3-year period and spanning a reef-wide thermal stress event. By the end of the sampling period, 28% (5/18) of corals in the fringing reef experienced partial mortality versus 78% (14/18) of corals in the forereef. Over 90% (50/54) of colonies had detectable dinoRNAV infections. Reef zone influenced the composition and richness of viral mcp amino acid types (‘aminotypes’), with the fringing reef containing the highest aminotype richness. The reef-wide thermal stress event significantly increased aminotype dispersion, and this pattern was strongest in the colonies that experienced partial mortality. These findings demonstrate that dinoRNAV infections respond to environmental fluctuations experienced in situ on reefs. Further, viral productivity will likely increase as ocean temperatures continue to rise, potentially impacting the foundational symbiosis underpinning coral reef ecosystems.

Mineralogical and geochemical characterization of the Wonosari formation limestone at Gunungkidul Indonesia as preliminary investigation of Portland cement raw material

Mineralogical and geochemical investigations were carried out to determine the characteristics of limestone in the Wonosari Formation and to suggest its depositional environment and a preliminary study for the properties of Portland Cement. The study was conducted at Ponjong Area, Gunungkidul Regency, Daerah Istimewa Yogyakarta, Indonesia. The outcrop in this study area is composed of Wonosari layered limestone. Based on the facies interpretation in the research area, there are three depositional units: packstone, wackstone, and grainstone. This area’s depositional environment is the back reef lagoon and sand apron zones that are not related to the open sea. According to XRF data, limestone showed a reasonably high composition of CaO compounds, about 53.78% - 59.09%. Most limestone samples were classified as good quality as Portland Cement Raw Material due to CaO content >49%. The MgO, SiO2, Al2O3, Fe2O3, SO3, K2O, Na2O, TiO2, and P2O5 compounds also fulfil the Indonesian National Standard (SNI-15-2049-2004). Geochemical content shows limestone has a higher grain percentage than the matrix. The utilization of limestone in research area are expected to be a reference for the development of natural resources in the future that pay attention to the environment for mutual sustainability.

Rudist reef structure: Insights from orientation of hippuritids at l'Espà (Campanian, southern Pyrenees)

Rudist were common Mesozoic reef builders, but rare examples exist to evaluate reef structure. The l'Espà locality (southern Pyrenees) is approached to study rudist reef structure by a quantitative assessment of the individuals' position (growing vs reworked) and its sedimentary context. This late Campanian reef is exposed along some 20 × 6 m outcrop. Builders are mainly Hippurites radiosus, although other rudists, such as Hippuritella lapeirousei, Hippuritella sp. and Mitrocaprina sp. are also present together with corals. The orientation of 325 specimens of mainly H. radiosus was plotted in stereographic and cartesian projections. Orientations and microfacies permit to differentiate 5 vertically stacked intervals (settings) along the outcrop: (1) Distal reef setting (reef-talus slope), with rudists reworked as large bunches of grouped specimens, with scarce erosion and preserving both valves articulated; (2) Halfway distal-proximal reef setting (close cluster reef), with abundant reworked, isolated, and flat-lying specimens. In this zone, endo-epibiont colonization on rudist shells is common, together with the presence of large (up to 1 m) branching and massive corals; (3) Proximal reef setting (frame/close cluster reef), where specimens are in life position; (4) A proximal back reef unit (spaced cluster reef) with few highly reworked specimens; (5) Distal back reef setting (very spaced cluster reef), with hardly any rudist fragments. This succession provides a reef tract model resembling that of most coral reefs and differs from smaller rudist reefs. The structure of the studied reef is well preserved as a result of high accommodation space related to thrust emplacement.