Background: Hemolysis is linked to an increase in the length of exposure to high shear stress in addition to the degree of shear stress. Turbulence is also a significant cause of blood injury. In the maglev ventricular assist device blood is only in contact with the pump housing and impeller. So, it is vital that decreasing NPSS, reducing exposure time, and the suppression of disturbed flow are incorporated into the optimization design for blood pump. The blood pump must also deliver enough pressure head and flow rate to sustain clinical circulation. The purpose of this study is to analyze the effects of different number of blades, number of splitters, blade length, blade angle and its distribution on pressure head, hemolysis and platelet activation. Firstly, computational fluid dynamics was used to analyze the effects of the above characteristics on scalar shear force, velocity distribution, hemolysis, and then the validity of the numerical analysis was verified by combining with in vitro hemolysis experiments, and finally the best impeller design was selected. Methods: In this study, three rotors with different number of vanes were developed. Compared to the CentriMag with curved vanes, all three variants have straight vanes to improve the pump efficiency and the passability in the pump. The inlet and outlet edges of the vanes are chamfered to reduce the shear stress area. In order to avoid excessive axial force makes the whole rotor in the rotation process floating, in the back cover plate position designed to raise the structure, in order to increase the surface area, reduce the axial force to stabilize the leaf top clearance, the three new design of the rotor back cover plate raised degree is also different. In order to balance the number of blades and the impeller’s hydraulic performance, the outer diameter of the impeller is also designed. 6-blade rotor consists of 3 blades and 3 spliters, the outer diameter of the impeller is 43.5mm; 8-blade rotor consists of 4 blades and 4 spliters, the outer diameter of the impeller is 43mm; 9-blade rotor consists of 3 blades and 6 spliters, the outer diameter of the impeller is 43mm. The 9-blade rotor consists of 3 blades and 6 splitters, with a minimum impeller outside diameter of 42.5mm. Results: By CFD simulation, the 9-blade maglev impeller outperformed several other impellers in terms of shear force, shear area, and passability, and the NIH in in vitro experiments was <0.0016g/100L Conclusion: The 9-vane magnetically levitated rotor developed in this study improves pump passability and reduces the occurrence of hemolysis in the pump by reducing the vane shear forceFigure 1. Independently developed magnetic levitation hemolysis platformFigure 2. Newly designed 3-bell bladeFigure 3. Computational fluid dynamics analysis of different impellers