This study examined the eiect of potential probiotic bacteria on growth and survival of the tilapia Oreochromis niloticus, under high density and suboptimum temperature. Presumptive Bacillus and lactic acid bacteria (LAB) were isolated from tilapia and from their culture system and were characterized for haemolytic and enzymatic activity, and antagonism against Vibrio. Selected strains were included in the diet of juvenile tilapia and evaluated during a 134day assay. The experiment was conducted with four treatments: (1) ¢sh fed with commercial feed plus Dry Oil s ; (2) ¢sh fed with commercial feed plus LAB; (3) ¢sh with bacilli in water; (4) ¢sh with a mixture of treatments 2 and 3. Tilapias in all treatments, including bacteria, grew signi¢cantly better than ¢sh fed with commercial feed plus Dry Oil s (control group). Survival was similar in all treatments. The physicochemical parameters of the culture system were maintained within the optimal ranges for the species, with the exception of temperature (19.9^24.821C). Animals fed diet supplemented with bacilli and LAB had good survival and the best growth performance, suggesting that bacteria are appropriate growth-stimulating additives in tilapia cultivation.