Baboons Papio Anubis Research Articles

A radioligand for in vitro autoradiography of CSF1R in post-mortem CNS tissues

BackgroundReactive microglia and recruited peripheral macrophages contribute to the pathogenesis of Alzheimer’s dementia (AD). Monocytes, macrophages and microglia all express the marker colony-stimulating factor 1 receptor (CSF1R). 4-Cyano-N-(4-(4-methylpiperazin-1-yl)-2-(4-methylpiperidin-1-yl)phenyl)-1H-pyrrole-2-carboxamide (1) is a high-affinity antagonist for CSF1R. We report the radiosynthesis of both [3H]1 and [11C]1. The PET imaging properties of [11C]1 in mice and baboon were investigated. [3H]1 was studied in Bmax measurement in post-mortem autoradiography in the frontal cortex, inferior parietal cortex and hippocampus from donors diagnosed with AD and age-matched controls. In vitro binding affinity of 1 was measured commercially. Nor-methyl-1 precursor was radiolabeled with [11C]iodomethane or [3H]iodomethane to produce [11C]1 and [3H]1, respectively. Ex vivo brain biodistribution of [11C]1 was compared in normal mice versus lipopolysaccharide-administered (LPS) murine model of neuroinflammation. Dynamic PET imaging was performed in a healthy male Papio anubis baboon. Post-mortem autoradiography with [3H]1 was performed in frozen sections using a standard saturation binding technique.ResultsCompound 1 exhibits a high in vitro CSF1R binding affinity (0.59 nM). [11C]1 was synthesized with high yield. [3H]1 was synthesized similarly (commercially). Biodistribution of [11C]1 in healthy mice demonstrated moderate brain uptake. In LPS-treated mice the brain uptake of [11C]1 was ~ 50% specific for CSF1R. PET/CT [11C]1 study in baboon revealed low brain uptake (0.36 SUV) of [11C]1. Autoradiography with [3H]1 gave significantly elevated Bmax values in AD frontal cortex versus control (47.78 ± 26.80 fmol/mg vs. 12.80 ± 5.30 fmol/mg, respectively, P = 0.023) and elevated, but not significantly different binding in AD hippocampus grey matter and inferior parietal cortex (IPC) white matter.ConclusionsCompound 1 exhibits a high in vitro CSF1R binding affinity. [11C]1 specifically labels CSF1R in the mouse neuroinflammation, but lacks the ability to efficiently cross the blood–brain barrier in baboon PET. [3H]1 specifically labels CSF1R in post-mortem human brain. The binding of [3H]1 is significantly higher in the post-mortem frontal cortex of AD versus control subjects.

Planum temporale grey matter volume asymmetries in newborn monkeys (Papio anubis).

The Planum temporale (PT) is one of the key hubs of the language network in the human brain. The gross asymmetry of this perisylvian region toward the left brain was considered as the most emblematic marker of hemispheric specialization of language processes in the brain. Interestingly, this neuroanatomical signature was documented also in newborn infants and preterms, suggesting the early brain's readiness for language acquisition. Nevertheless, this latter interpretation was questioned by a recent report in non-human primates of a potential similar signature in newborn baboons Papio anubis based on PT surface measures. Whether this "tip of the iceberg" PT asymmetry is actually reflecting asymmetry of its underlying grey matter volume remains unclear but critical to investigate potential continuities of cortical specialization with human infants. Here we report a population-level leftward asymmetry of the PT grey matter volume in in vivo 34 newborn baboons P. anubis, which showed intra-individual positive correlation with PT surface's asymmetry measures but also a more pronounced degree of leftward asymmetry at the population level. This finding demonstrates that PT leftward structural asymmetry in this Old World monkey species is a robust phenomenon in early primate development, which clearly speaks for a continuity with early human brain specialization. Results also strengthen the hypothesis that early PT asymmetry might be not a human-specific marker for the pre-wired language-ready brain in infants.

Interactions Between People and Nigeria-Cameroon Chimpanzee (Pan troglodytes ellioti) Around Mbam-Djerem National Park, Central Cameroon

Understanding the interactions between local people and chimpanzees is crucial to develop sustainable wildlife conservation strategies and management policy in the Mbam-Djerem National Park (MDNP). The MDNP covers about 416,512 ha and shelters the Nigeria-Cameroon chimpanzee ( Pan troglodytes ellioti) being the most endangered of all currently recognized chimpanzee subspecies. Close to 30,000 people live in the periphery of the MDNP and depend on the park’s resources for subsistence. We investigated the extent of, and factors leading to, the interactions between people and chimpanzees through a socio-economic survey focusing on households (124) and individual interviews (38) in 30% of villages around MDNP. About 95% of the respondents stated that human–wildlife conflict is an issue around MDNP. Access to resources (78.9%), crop damage (84%), and animal attack (11.3%) were the main sources of conflicts. The ground squirrel Xerus erythropus (59.7%), the green monkey Chlorocebus aethiops (20.2%), chimpanzees (7.3%) and baboons Papio anubis (5.6%) were identified as the main conflict animals and the destroyed crops included peanuts (70.2%), maize (15%) and cassava (5%). The population perceptions differed between the park’s four sectors and were significantly influenced by education and the main livelihood activity. The study also provided quantitative evidence on the role of personal and religious beliefs on the behavior towards chimpanzees. Our findings suggest that the improvement of income-generating activities and education will contribute to strengthening the conservation of chimpanzees.

Modelling and inference for the movement of interacting animals

Abstract Statistical modelling of animal movement data is a rapidly growing area of research. Typically though, these models have been developed for analysing the tracks of individual animals and we lose sight of the impact animals have on each other with regards to their movement behaviours. We aim to develop a model with a flexible social framework that allows us to capture that information. Our approach is based on the concept of social hierarchies, and this is embedded in a multivariate diffusion process which models the movement of a group of animals. The possibility of switching between behavioural states facilitates dynamic social behaviours and we augment the observed data with sampled state switching times in order to model the animals' behaviour naturally in continuous time. In addition, this enables us to carry out exact inference in a Bayesian setting with the benefits of being able to handle regular, irregular and missing data. All movement and behaviour parameters are estimated with Markov chain Monte Carlo methods. We examine the capability of our model with simulated data before fitting it to GPS locations of five wild olive baboons Papio anubis. The results enable us to identify which animals are influencing the movement of others and when, which provides both a dynamic and long‐term static insight into the group's social behaviours. Our model offers a flexible method in continuous time with which to model the network of social interactions within animal movement. Doing so avoids the limitations caused by a discrete‐time approach and it allows us to capture rich information with regards to a group's social structure, leading to constructive applications in conservation and management decisions. However, currently it is a computationally expensive task to fit the model to data, which in turns limits extending the model to more fruitful but complex cases such as heterogeneity in space or individual characteristics. Furthermore, our social hierarchy approach assumes all relevant animals are tracked and that any interactions have some ordering, both of which narrow the scope within which this approach is appropriate.

Gestural communication in olive baboons (Papio anubis): repertoire and intentionality.

Gesturing is a widespread phenomenon in the animal kingdom, as well as an important facet of human language. As such, studying the communicative gestures of our close phylogenetic relatives is essential to better understand its evolution. While recent studies have shown that ape gestural communication shares some properties with human language, very little is known about the properties of gestural communication in monkeys. The aims of this study were to establish the first quantitative repertoire of gestural communication in a species of old-world monkeys, the olive baboon Papio anubis, and to determine its properties in terms of variability, flexibility, and intentionality. Gestural communication was continuously recorded on 47 captive olive baboons over 1year. Their gestural repertoire was composed of 67 visual, tactile, and audible gestures, which were used flexibly across different contexts, indicating means-ends dissociation. We found that the use of gestures was variable across individuals and ages, notably with repertoire size decreasing with age. Baboons used their gestures intentionally; gesturers looked at the recipient, waited for a response, and took into account the attentional state of their recipient. Particularly, they actively adjusted the modality of their gesture to the recipient's visual attention, using more visual gestures when the recipient was attending and more tactile gestures when the recipient was not. Thus, the gestural communicative system of olive baboons possesses properties which are similar to the ones of apes and to human language. These intentional features of gestural communication, that may constitute a prerequisite of language evolution, may have been present in the common ancestor of baboons and humans, around 30-40 million years ago.

Encounter rates and group sizes of diurnal primate species of Mole National Park, Ghana

Primate species are not widely explored in Ghana’s savannah ecosystems. We report data on encounter rates and group sizes of primates at the Mole National Park in Ghana. Forty transects, each of 5km length, were randomly laid in the park for the study. Four species of primates were visually recorded during field surveys: Olive Baboon Papio anubis, Patas Monkey Erythrocebus patas, Green Monkey Chlorocebus sabaeus and Colobus vellerosus. The status of C. vellerosus is Critically Endangered, the status of the other species is Least Concern according to the IUCN Red List. Encounter rates (groups/km) were 0.98, 0.65 and 0.45 for Olive Baboons, Patas Monkeys and Green Monkeys respectively. The mean group sizes were: Olive Baboon 10.8 (SE=1.1, range=1-38), Patas Monkey 12.2 (SE=3.3, range=1-35), and Green Monkey 10.0 (SE=1.9, range=1-25). Only one group of White-thighed Colobus with a group size of six was encountered. Encounter rates and group sizes of the same species varied in different parts of the park, and factors such as resource distribution and security against secret hunting may have influenced this variation. Authors recommend further studies to facilitate better understanding of these primates.

Imaging α4β2 Nicotinic Acetylcholine Receptors (nAChRs) in Baboons with [18F]XTRA, a Radioligand with Improved Specific Binding in Extra-Thalamic Regions.

Currently available positron-emitting radiotracers for imaging of the α4β2 subtype of nicotinic acetylcholine receptors (nAChRs) exhibit high and moderate specific binding in the thalamus and extra-thalamic brain regions, respectively. In many neuropsychiatric disorders, α4β2-nAChRs are altered in the extra-thalamic brain regions, but not necessarily in the thalamus. The purpose of this study was to evaluate [18F]XTRA, a new α4β2-nAChR positron emission tomography (PET) radioligand with improved specific binding in extra-thalamic brain regions, in non-human primates. The regional distribution of [18F]XTRA in the brain of Papio anubis baboons was evaluated in baseline and blocking experiments. Various PET modeling procedures were used for determination of volume of distribution (V T), binding potential (BPND), and receptor occupancy. Radiation dosimetry for [18F]XTRA was studied in male CD-1 mice and extrapolated to human dosimetry estimates using OLINDA/EXM software. [18F]XTRA was synthesized using an automated radiochemistry module with 25% decay-corrected radiochemical yield. [18F]XTRA readily enters the baboon brain and specifically labels α4β2-nAChRs. Mathematical modeling demonstrates high binding potential values (BPND=7 and 1.3 in the thalamus and frontal cortex, respectively). A PET scanning time of 90-120min was sufficient to obtain stable V T values in the extra-thalamic regions. The extrapolated human effective dose was 0.041mSv/MBq (0.15Rem/mCi). [18F]XTRA exhibits improved specific binding in the baboon brain including extra-thalamic regions and it is considered radiologically acceptable for human studies. Further evaluations of [18F]XTRA in human subjects are under way.

Differential influence of propofol and isoflurane anesthesia in a non-human primate on the brain kinetics and binding of [(18)F]DPA-714, a positron emission tomography imaging marker of glial activation.

Translocator protein 18 kDa (TSPO) expression at the mitochondrial membrane of glial cells is related to glial activation. TSPO radioligands such as [(18)F]DPA-714 are useful for the non-invasive study of neuroimmune processes using positron emission tomography (PET). Anesthetic agents were shown to impact mitochondrial function and may influence [(18)F]DPA-714 binding parameters and PET kinetics. [(18) F]DPA-714 PET imaging was performed in Papio anubis baboons anesthetized using either intravenous propofol (n = 3) or inhaled isoflurane (n = 3). Brain kinetics and metabolite-corrected input function were measured to estimate [(18) F]DPA-714 brain distribution (VT). Displacement experiments were performed using PK11195 (1.5 mg/kg). In vitro [(18)F]DPA-714 binding experiments were performed using baboon brain tissue in the absence and presence of tested anesthetics. Brain radioactivity peaked higher in isoflurane-anesthetized animals compared with propofol (SUVmax = 2.7 ± 0.5 vs. 1.3 ± 0.2, respectively) but was not different after 30 min. Brain VT was not different under propofol and isoflurane. Displacement resulted in a 35.8 ± 8.4% decrease of brain radioactivity under propofol but not under isoflurane (0.1 ± 7.0%). In vitro, the presence of propofol increased TSPO density and dramatically reduced its affinity for [(18)F]DPA-714 compared with control. This in vitro effect was not significant with isoflurane. Exposure to propofol and isoflurane differentially influences TSPO interaction with its specific radioligand [(18)F]DPA-714 with subsequent impact on its tissue kinetics and specific binding estimated in vivo using PET. Therefore, the choice of anesthetics and their potential influence on PET data should be considered for the design of imaging studies using TSPO radioligands, especially in a translational research context.

Primate Conservation in the Rangeland Agroecosystem of Laikipia County, Central Kenya

Maintenance of the diversity of primates depends not only on the conservation of protected areas, but also on the con- servation of areas that lack formal protection and are occupied by people, crops, and/or livestock. Livestock rangelands, when well-managed, can support viable populations of primates. This article describes (1) the primate community in the rangeland agro- ecosystem of Laikipia County, central Kenya, (2) how primates use this agroecosystem, (3) the importance of this agroecosystem to the primates of Laikipia, and (4) the threats to these primates. Patas monkeys Erythrocebus patas, olive baboons Papio anubis, vervet monkeys Chlorocebus pygerythrus, and northern lesser galagos Galago senegalensis in the Laikipia rangeland agroecosys- tem benefit from man-made perennial water sources, habitat protection, reduced large predator densities, and an array of research and conservation activities. The level of conflict between humans and non-human primates in this rangeland agroecosystem is low relative to that in neighboring cropland agroecosystems. The main threats are habitat fragmentation, degradation and loss, and the decline of perennial water sources. Hunting is not a serious threat to primates in Laikipia. Erythrocebus patas is the most threatened primate in Laikipia and the one least tolerant of humans and habitat degradation and loss. Habitat conservation in Laikipia should focus on water-associated vegetation types and the adjacent whistling thorn Acacia drepanolobium woodlands, particularly along the Ewaso N'yiro River and its major tributaries.

Associations between spatial position, stress and anxiety in forest baboons Papio anubis

Spatial position within a group affects the value of group-living benefits such as reduced predation risk and improved foraging. The threat of predation, poor nutrition or increased competition from conspecifics can all cause stress. In many species, central positions are known to be more beneficial than peripheral positions in terms of reduced predation, vigilance and foraging. In this study, we examine whether spatial position within a group is associated with stress and anxiety in a troop of olive baboons (Papio anubis). We predicted that the benefits of occupying central positions would be reflected by a reduction in stress and anxiety for animals who spent the most time in the centre of the group. The study subjects appeared to compete actively for the centre of the group. Physiological stress measures (faecal glucocorticoid concentrations) were positively correlated with time spent in central positions. Time spent in central positions was positively correlated with proximity but negatively correlated with vigilance behaviours (alarm barks). Vigilance rates were positively correlated with measures of anxiety (self-scratch frequency). It is suggested that individuals experience chronic stress due to proximity to conspecifics in central positions, whilst perceived predation risk causes anxiety, with perceived predation risk experienced more by individuals on the periphery.

First D1-like receptor PET imaging of the rat and primate kidney: implications for human disease monitoring.

The intrarenal dopamine system is important for signaling and natriuresis, and significant dysfunction is associated with hypertension and kidney disease in ex vivo studies. Dopamine receptors also modulate and are modulated by the renin-angiotensin-aldosterone system. Here, we show the first in vivo measurement of D1-like receptors in the renal cortex of Sprague-Dawley rat and Papio anubis baboon using [(11)C]NNC 112, a positron emission tomography radioligand for D1-like receptors. In addition, we show a D1-like binding potential response to angiotensin II blockade in rats using losartan. Demonstration of self-saturable binding in the rat as well as specific and saturable binding in Papio anubis validate the use of [(11)C]NNC 112 in the first in vivo measurement of renal dopamine D1-like receptors. Furthermore, [(11)C]NNC 112 is a radioligand tool already validated for use in probing human central nervous system (CNS) D1-like receptors. Our work demonstrates specific and saturable non-CNS binding in higher animals and the ability to quantify physiological response to drug treatment and provides a clear path to extend use of [(11)C]NNC 112 to study renal dopamine in humans.

Chimpanzees in mantraps: lethal crop protection and conservation in Uganda

AbstractA main concern of farmers worldwide is how to reduce crop losses to wildlife. Some potentially lethal crop protection methods are non-selective. It is important to understand the impact of such methods on species of conservation concern. Uganda has important populations of Endangered eastern chimpanzeesPan troglodytes schweinfurthii. Farmers sometimes use large metal mantraps to guard their fields against crop-raiding wildlife, particularly baboonsPapio anubisand wild pigsPotamochoerussp.. Chimpanzees that range onto farmland also step in these illegal devices and without rapid veterinary invention face severe injury or eventual death. Unlike inadvertent snaring of great apes in African forests, the problem of mantraps in forest–farm ecotones has received little attention. We report 10 cases of entrapped chimpanzees in the cultivated landscape surrounding Uganda's Budongo Forest during 2007–2011, undoubtedly only a portion of the actual number of cases. Mantraps currently present a substantial threat to ape populations in this important conservation landscape. Our data underscore the need for conservation programmes to consider the techniques used by rural farmers to protect their livelihoods from wild animals.

Characterisation of [11C]PR04.MZ in Papio anubis baboon: A selective high-affinity radioligand for quantitative imaging of the dopamine transporter

N-(4-fluorobut-2-yn-1-yl)-2β-carbomethoxy-3β-(4'-tolyl)nortropane (PR04.MZ, 1) is a PET radioligand for the non-invasive exploration of the function of the cerebral dopamine transporter (DAT). A reliable automated process for routine production of the carbon-11 labelled analogue [(11)C]PR04.MZ ([(11)C]-1) has been developed using GMP compliant equipment. An adult female Papio anubis baboon was studied using a test-retest protocol with [(11)C]-1 in order to assess test-retest reliability, metabolism and CNS distribution profile of the tracer in non-human primates. Blood sampling was performed throughout the studies for determination of the free fraction in plasma (f(P)), plasma input functions and metabolic degradation of the radiotracer [(11)C]-1. Time-activity curves were derived for the putamen, the caudate nucleus, the ventral striatum, the midbrain and the cerebellum. Distribution volumes (V(T)) and non-displaceable binding potentials (BP(ND)) for various brain regions and the blood were obtained from kinetic modelling. [(11)C]-1 shows promising results as a selective marker of the presynaptic dopamine transporter. With the reliable visualisation of the extra-striatal dopaminergic neurons and no indication on labelled metabolites, the tracer provides excellent potential for translation into man.

Human–carnivore conflict around Pendjari Biosphere Reserve, northern Benin

AbstractClose proximity between humans and large predators results in high levels of conflict. We investigated the extent of, and factors leading to, this conflict through focal group and individual interviews in all villages around Pendjari Biosphere Reserve, northern Benin. Livestock losses from 2000 to 2007 (n = 752) were reported to be mainly caused by spotted hyaena Crocuta crocuta (53.6%), baboon Papio anubis (24.8%), and lion Panthera leo (18.0%). These predators mainly predated sheep and goats (52.1%) and pigs (42.3%), with lions being the main predators of cattle (78.9%). Lion and hyaena diets were more diverse than that of baboons, which killed only small stock. The level of conflict increased during 2000–2007. Predation rate differs between predator species and is significantly influenced by month, rainfall of the month before the predation event, and length of the dry period in a year. The geographical position of the village, the distance of the village to the Park and the number of herbivores legally killed every hunting season also influenced predation intensity. Our findings suggest that improvement of husbandry techniques and education will reduce conflicts and contribute to improved conservation of these threatened predators.

An incident of swimming in a large river by a mantled howling monkey (<i>Alouatta palliata</i>) on the north coast of Honduras

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Coagulation cascade activation triggers early failure of pig hearts expressing human complement regulatory genes

Hyperacute rejection (HAR) and early graft failure (EGF) have been described in a minority of pig-to-baboon heart transplants using organs transgenic for human complement regulatory proteins (hCRP). Here we investigate the role of coagulation cascade activation in the pathogenesis of HAR and EGF in a consecutive series where a high incidence of these outcomes was observed. Twenty-eight naïve wild-caught Papio anubis baboons received heterotopic heart transplants from pigs transgenic for hDAF (n = 23) or hMCP (n = 5). Immunosuppression consisted of cyclosporine A, cyclophosphamide and MMF (n = 18) or anti-CD154 mAb (IDEC-131) and ATG (n = 10). Eleven received anti-Gal carbohydrates (GAS914, n = 8, or NEX1285, n = 3), of which four also underwent extracorporeal immunoadsorption (EIA), and 12 also received pharmacologic complement inhibitors (C1 INH, n = 9, or APT070, n = 3). Excluding one technical failure, 14 of 27 transplants (11 hDAF, 3 hMCP) exhibited either HAR (n = 10) or EGF (n = 4). Surprisingly, neither complement inhibition (with C1 INH or APT070) nor anti-Gal antibody depletion with GAS914, NEX1285, or additional EIA consistently prevented HAR or EGF despite low or undetectable complement deposition. Strikingly, most grafts with HAR/EGF exhibited prominent fibrinogen and platelet deposition associated with systemic coagulation cascade activation, consistent with non-physiologic intravascular coagulation, in many instances despite little evidence for antibody-mediated complement activation. We conclude that dysregulated coagulation correlates closely with and probably causes primary failure of pig hearts transgenic for hCRP. These data support efforts to define effective strategies to prevent dysregulated coagulation in pig organ xenografts.

Patterns of crop raiding by primates around the Budongo Forest Reserve, Uganda

Crop raiding by primates in particular and wild animals in general is a significant source of people-forest conflict around the Budongo Forest Reserve, Uganda. Crop loss to wild animals undermines local support for conservation efforts in this area. Patterns of primate crop raiding were studied over a period of 14 months in six villages (five adjacent to the Budongo Forest Reserve and one that is approximately 3,500 m from the forest edge). Data were collected via a questionnaire survey. Additional information was obtained from the relevant local government offices. Chimpanzees Pan troglodytes, baboons Papio anubis, other monkeys, bush pigs Potamochoeus procus and porcupines Hystrix cristata were reported by farmers to be the major causes of crop losses by wildlife. Of farmers, 73% reported suffering crop damage caused by primates, and 79% considered baboons to be the most destructive of all crop raiding species. Drought, insect pests, poor sowing, plant diseases and accidental fires were other sources of crop losses to farmers, though the risk of crop damage particularly by primates is perceived as the most serious potential cause of losses. Using chimpanzees as a case study, patterns of crop damage across the year are compared with seasonal fluctuations in availability of wild foods.

Platelet Rich Plasma (PRP)

Boyne PJ, Nath R, Nakamura A: Human recombinant BMP-2 in osseous reconstruction of simulated cleft palate defects. Br J Oral Maxillofac Surg 36:84, 1998 Boyne PJ: Effect of long-term function on BMP complemented distraction osteogenesis of the edentulous mandible in the Papio anubis baboons. Proceedings of the 4th International Congress on Maxillofacial and Craniofacial Distraction Osteogenesis (Abstract #S01-16), Paris, July 2-5, 2003 Boyne PJ, Mark RE, Nevins M, et al: A feasibility study evaluating rhBMP-2/absorbable collagen sponge for maxillary sinus floor augmentation. Int J Periodont Restor Dent 17:11, 1997

Distraction osteogenesis of the nasal and antral osseous floor to enhance alveolar height

The purpose of the study was to obtain information on the possibility of using distraction osteogenesis (DO) to increase the height of posterior atrophic maxillary alveolar ridges by distracting very small alveolar crest bone segments and, secondly, to determine if it is possible to distract the bony antral floor itself, producing new bone between the sinus membrane and the alveolar crest. Three adult Papio anubis baboons were rendered edentulous in the posterior maxilla. Bilateral osteotomies were performed to produce a thin margin of crestal alveolar bone and in one area, a complete exposure of the antral floor. Distractors were placed against the antral and nasal floors and the thin 1- x 1.5-mm thick alveolar bone crest was distracted 10 mm over a period of 10 days. Animals were labeled with tetracycline at 3 and 4 months and were sacrificed at 5 months. A complete osseous regeneration of the nasal floor and alveolar ridge greater than 10 mm in height was produced in all cases. Histomorphometric analysis of the DO-induced osseous areas revealed cancellous bone with increasing thickness during the third and fourth month and formation of new bone cortices increasing in thickness during the same time. Significant new bone also formed in the antral floor areas that had been purposely interdicted by the osteotomies. The results of this study indicate that very small segments of bone of the posterior maxilla can be distracted to produce significant increases in the alveolar bone height and that a new osseous nasal-antral floor enhancement can be produced by this technique.

Effect of long-term function on BMP- complemented distraction osteogenesis of the edentulous mandible in the Papio anubis baboon

Effect of long-term function on BMP- complemented distraction osteogenesis of the edentulous mandible in the Papio anubis baboon