Babassu Oil Research Articles

Study of the Feasibility of Extracting Babassu Seed Oil by Extraction in an Ultrasonic Bath Using Organic Solvents

Introduction: Orbignia speciosa or phalerata (babassu) is an oilseed plant of great economic importance. The structure of the babassu fruit is similar to that of a coconut, and is composed of four parts: epicarp, mesocarp, endocarp and the seed, which contains approximately 60% oil, where lauric acid predominates in its composition, which is mainly responsible for the antiviral, antifungal and bactericidal activities of babassu oil. Babassu oil is a vegetable oil widely used in the food industry, and it also has wide application in the cosmetic, pharmaceutical and chemical industries. The objective of this work will be to use organic solvents in the extraction of oil from the babassu seed by the ultrasonic bath method with hexane, and with less toxic solvents such as ethyl acetate, isopropanol and ethanol. The kinetic curve of the extraction process will be determined to define the time required to reach saturation, establishing the time found as the ideal for the process. The fatty acid composition will be determined by gas chromatography. The antioxidant activity will be analyzed according to the adapted method of free radical capture by ultraviolet and visible spectrophotometry (UV-VIS). This method is based on the capture of the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical by antioxidants, producing a decrease in absorbance. After the interpretation and correlation of the results, it will be possible to establish the factors that present the best performance for the extraction of babassu oil and its chemical properties and compare them with the oil obtained using hexane. Objective: The objective of this study is to investigate the extraction of babassu seed oil with different organic solvents in order to evaluate the possibility of replacing hexane with a less toxic alternative solvent using the ultrasound extraction method. Theoretical Framework: A large amount of waste is produced by the food and agricultural industries, such as pulp, skins, seeds and fruit peels, some of which are considered by-products with a certain economic value. Babassu seed oil is rich in lauric acid, which has anti-inflammatory and immunomodulatory activities, among others. Method: The methodology used is experimental and applied in stages; the ideal oil extraction conditions were verified, the extraction was carried out using different organic solvents, the oil content obtained was quantified, the fatty acids were characterized, the antioxidant activity was determined using the DPPH method and finally the results obtained were compared. Results and Discussion: The oils obtained from the babassu seed through extraction in an ultrasonic bath with different organic solvents proved to be a promising by-product due to the characteristics evaluated. Research Implications: The oils obtained through organic solvents presented similar characteristics to those obtained with hexane, thus enabling its replacement due to it being a more toxic solvent. Originality/Value: This study contributes to the literature on vegetable oils extracted through green technologies with organic solvents, adding value to a by-product of a native fruit.

Ultrasound assisted interesterification of babassu oil with acetates using acid catalysts for biodiesel and triacetin production

Ultrasound assisted interesterification of babassu oil with acetates using acid catalysts for biodiesel and triacetin production

Solid Lipid Nanoparticles Based on Babassu Oil and Copaiba Oleoresin: A Promising Approach for Prostate Cancer Therapy.

Solid lipid nanoparticles (SLNs) represent promising nanostructures for drug delivery systems. This study successfully synthesized SLNs containing different proportions of babassu oil (BBS) and copaiba oleoresin (COPA) via the emulsification-ultrasonication method. Before SLN synthesis, the identification and quantification of methyl esters, such as lauric acid and β-caryophyllene, were performed via GC-MS analysis. These methyl esters were used as chemical markers and assisted in encapsulation efficiency experiments. A 22 factorial design with a center point was employed to assess the impact of stearic acid and Tween 80 on particle hydrodynamic diameter (HD) and polydispersity index (PDI). Additionally, the effects of temperature (8 ± 0.5 °C and 25 ± 1.0 °C) and time (0, 7, 15, 30, 40, and 60 days) on HD and PDI values were investigated. Zeta potential (ZP) measurements were utilized to evaluate nanoparticle stability, while transmission electron microscopy provided insights into the morphology and nanometric dimensions of the SLNs. The in vitro cytotoxic activity of the SLNs (10 µg/mL, 30 µg/mL, 40 µg/mL, and 80 µg/mL) was evaluated using the MTT assay with PC-3 and DU-145 prostate cancer cell lines. Results demonstrated that SLNs containing BBS and COPA in a 1:1 ratio exhibited a promising cytotoxic effect against prostate cancer cells, with a percentage of viable cells of 68.5% for PC-3 at a concentration of 30 µg/mL and 48% for DU-145 at a concentration of 80 µg/mL. These findings underscore the potential therapeutic applications of SLNs loaded with BBS and COPA for prostate cancer treatment.

Development of artificial neural network models to predict the concentration range of formation of microemulsions containing babassu oil

Microemulsions have gained prominence in the research for biomolecules nanocarriers due to their thermodynamic stability and auto-organization. However, the formation of these systems requires a high experimental effort. To minimise it, an Artificial Neural Network to predict the concentration range of microemulsion formation was developed using weight fraction, hydrophilic-lipophilic balance as model input. We also evaluated the effect of including surfactant viscosity as input. Experimental data were generated using formulations with babassu oil, water, Tween® 80, and Labrasol®. After training, the proposed model presented accuracies up to 93%, with the addition of viscosity reducing the cross-validation variance.

Enhancing biocatalyst performance through immobilization of lipase (Eversa® Transform 2.0) on hybrid amine-epoxy core-shell magnetic nanoparticles

Magnetic nanoparticles were functionalized with polyethylenimine (PEI) and activated with epoxy. This support was used to immobilize Lipase (Eversa® Transform 2.0) (EVS), optimization using the Taguchi method. XRF, SEM, TEM, XRD, FTIR, TGA, and VSM performed the characterizations. The optimal conditions were immobilization yield (I.Y.) of 95.04 ± 0.79 %, time of 15 h, ionic load of 95 mM, protein load of 5 mg/g, and temperature of 25 °C. The maximum loading capacity was 25 mg/g, and its stability in 60 days of storage showed a negligible loss of only 9.53 % of its activity. The biocatalyst demonstrated better stability at varying temperatures than free EVS, maintaining 28 % of its activity at 70 °C. It was feasible to esterify free fatty acids (FFA) from babassu oil with the best reaction of 97.91 % and ten cycles having an efficiency above 50 %. The esterification of produced biolubricant was confirmed by NMR, and it displayed kinematic viscosity and density of 6.052 mm2/s and 0.832 g/cm3, respectively, at 40 °C. The in-silico study showed a binding affinity of −5.8 kcal/mol between EVS and oleic acid, suggesting a stable substrate-lipase combination suitable for esterification.

Effect of temperature on the physical properties’ density, viscosity, and refractive index of babassu (Orbignya sp.) oil in the range between 293 and 333 K

The investigation of vegetable oils' physical properties is indispensable to promote further applicability. In this sense, the density (ρ), absolute viscosity (µ), and refractive index (IR) of two brands of refined babassu oil (A and B) were obtained as a function of temperature (293.15 to 333.15 K). Mathematical models were adjusted to the experimental data. Babassu oil had an average percentage of saturated fatty acids of 85.60%. The ρ, µ and IR were inversely proportional to temperature and were influenced by the fatty acid composition and the degree of unsaturation of the oil. Arrhenius Model was the most accurate in explaining the variation of µ as a function of temperature. Time-dependent properties differed between samples, which highlights the importance of investigating different sources. The models obtained are useful for the disclosure of the physical properties of babassu oil, enabling its application in engineering processes and product development.

Feeding Behavior, Water Intake, and Physiological Parameters of Feedlot Lambs Fed with Diets Containing Babassu Oil Associated with Sunflower Oil Blend.

This study aimed to investigate the impact of dietary inclusion of babassu oil (BO) associated with sunflower oil (SO) on feeding behavior, water intake, and physiological parameters of feedlot lambs. Thirty-five castrated male lambs (16.6 kg ± 3.9 kg) were distributed in a randomized block design with 5 treatments (diets) and 7 replications. The tested diets were oil-free diet (OF), 45 g/kg BO (BO), 30 g/kg BO with an additional 15 g/kg SO (1.5 SO), 22.5 g/kg BO with an additional 22.5 g/kg SO (2.25 SO), and 30 g/kg SO with an additional 15 g/kg BO (3.0 SO) on dry matter (DM) basis. The experimental period lasted 60 days. Animals that received BO diet and the combination of BO with SO had lower intakes of DM and neutral detergent fiber (NDF) compared to the control diet (P < 0.05). Differences on the respiratory rate (RR) was observed between animals in the control diet and those in the diets containing SO (P=0.001), with a linear increase in RR as the levels of SO in the diets increased (P=0.004). All physiological parameters showed a time effect (P < 0.05). Animals fed with the control diet had higher water intake via drinking fountain (P=0.030) and total water intake (P=0.029) compared to animals fed with diets containing SO. In relation to SO levels, water intake via drinking fountain (P=0.002), total water intake (P=0.002), and total water intake per kg of DM ingested (P=0.001) linearly increased with the levels increase in the composition of the diets. The tested diets did not alter the feeding behavior of the feedlot lambs. However, the combination of BO with different levels of SO reduced DM and water intake via drinking fountain and RR.

Aumento da estabilidade de carambolas com uso de coberturas de amido e óleo de babaçu com plastificantes

Abstract The objective of this work was to determine the effect of edible coatings made with babassu (Orbignya phalerata) starch and oil, combined with different plasticizers, on the shelf life of star fruit (Averrhoa carambola). The experimental design was completely randomized, in a 5×6 factorial arrangement, with five treatments and six storage periods, with ten replicates per treatment in each storage period. The fruits were stored for 20 days at 10°C and a relative humidity of 65-70%. Five treatments were evaluated: four coated ones (with starch+glycerol, starch+mannitol, starch+oil+glycerol, and starch+oil+mannitol) and one uncoated (control). The studied variables were: mass loss, pH, titratable acidity, soluble solids, ascorbic acid, and color. Mass loss, pH, soluble solids, redness, and yellowness increased linearly during storage, whereas titratable acidity, ascorbic acid, and luminosity decreased linearly. The starch+mannitol treatment stood out, presenting the highest luminosity and the best interaction with babassu oil, which reduced fruit yellowness. However, this interaction caused a lower adherence of the plasticizer to the peel of the fruit, not slowing its ripening down. The use of the starch+mannitol coating reduced mass loss and polysaccharide conversion into soluble sugars. Therefore, this treatment is a promising eco-friendly technique for an improved postharvest storage of star fruit.

Bioactive compounds and benefits of by-products of Amazon babassu oil production: potential for dietary supplement, biomedical and food applications.

Babassu coconut (Attalea speciosa syn. Orbignya phalerata) contains an oil-rich nut and is primarily found in South America's Amazon region. Future market researchers predict an increase in the babassu oil market from USD 227.7 million in 2022 to USD 347.0 million by 2032, and the yield of babassu oil from babassu-processed waste could reach 90%. Of these, mesocarp flour is an underrated by-product used only for animal feed purposes by local producers. This comprehensive review focuses on advances in knowledge and understanding of phytochemicals from babassu oil by-products considering the mechanisms of action - covering antioxidant, antimicrobial, antiparasitic, anti-inflammatory, antithrombotic, immunomodulatory, and anticancer effects. Babassu coconut fruit contains free fatty acids, (poly)phenols, phytosterols, and triterpenes. Pytochemicals, antiparasitic and antibacterial activities of babassu mesocarp flour were shown, but fungi and viruses can get more attention. Beyond its antioxidant capacity, babassu mesocarp flour showed potential as a dietary food supplement. Aqueous suspensions of mesocarp flour with a higher preference for cancer cells than normal cells and an antithrombotic effect were also identified, probably related to the antioxidant capacity of its secondary metabolites. Mesocarp flour, a starch-rich fraction, is promising for application as biodegradable packaging to improve the oxidative stability of foods. Finally, low-added value fractions can be considered bio-waste/co-products, and their phytochemicals may attract interest for applications in medicine and nutrition. Toxicological concerns, trends, and gaps are discussed for the future of foods and related sciences.

Determination of the Effect of the Addition of Second-Generation Biodiesel BBuE to Diesel Fuel on Selected Parameters of “B” Fuels

Progressive and increasingly noticeable climate change is forcing the search for new energy sources to reduce greenhouse gas emissions, especially carbon dioxide. One way to reduce greenhouse gas emissions is by gradually replacing fossil fuels with biofuels. The authors of this work addressed the production of second-generation biofuel. The purpose of this study was to produce second-generation biodiesel from babassu palm oil (BBuE) and first-generation biodiesel from rapeseed oil (RME), to study their properties, and to determine the effect of the addition of these biodiesels to diesel fuel on selected properties of “B” fuels that affect the fuel–air mixture formation process and the combustion process. Biodiesel from babassu oil was produced because it is non-edible and has a different composition than canola oil. Then, fuels were prepared that were mixtures of diesel oil and biodiesel containing from 10 to 40% (v/v) BBuE or RME (B10, B20, B30, and B40).Tests were conducted on selected physicochemical properties of the obtained fuels. “B” fuels prepared with BBuE and DF were shown to have more favorable fuel properties than those obtained from RME and DF. Fuels that are blends of BBuE and DF have slightly higher heating values, higher cetane number values, a more favorable distillation curve, lower dynamic viscosity values as a function of temperature, and marginally lower flash point values compared to the corresponding blends of RME and DF. Esters with shorter molecules have been shown to have more favorable fuel properties.

Effectiveness of babassu starch and oil coating with plasticizers to improve shelf life of eggs

Effectiveness of babassu starch and oil coating with plasticizers to improve shelf life of eggs

Scientific and technological advancements in the utilisation of by‐products from babassu oil extraction: a bibliometric review

SummaryBabassu is a tropical palm native in South and Central America that produces high‐quality oil from its fruit. The babassu oil extraction results in the production of babassu coconut by‐products (BCBs) – epicarp, mesocarp, endocarp, and almond cake. In this study, we utilised bibliometric analysis and literature review to understand and evaluate their potential for food applications. Our analysis revealed a total of 165 studies published between 1945 and 2022 in the Web of Science database. Notably, Brazil emerged as a prominent contributor to research in this field. The babassu epicarp and endocarp were identified as lignocellulosic materials. Although they are not directly consumable, they possess the potential to serve as sustainable sources of bioactive compounds. In contrast, the babassu mesocarp primarily consists of starch, while the babassu cake exhibits a high protein content. These ingredients play pivotal roles in bakery products and plant‐based formulations, respectively. Despite these findings, our investigation uncovered a knowledge gap regarding the assessment of these fractions in the context of food science and nutrition. By addressing this research gap, we aspire to contribute to the exploration and use of the full potential of BCBs, promoting their integration into the food sector.

Characterization of Babassu Mesocarp Flour as Potential Bio-Reinforcement for Poly(Lactic Acid)

Growing environmental concern has resulted in a strong trend towards using green materials, and the efficient use of by-products reduces resource waste and environmental pollution. Using Babassu Mesocarp Flour (BMF), a by-product of the babassu oil extraction industry, as bio-reinforcement for polymers, such as Poly (Lactic Acid) (PLA), is a promising alternative. Before any attempt to develop composite films, the industrially extracted BMF was characterized to know its chemical composition, particle size distribution, specific surface area, morphology, and structure, which is the main objective of this manuscript. The elemental analysis showed that the BMF is predominantly organic and also showed contents of inorganic elements by XRF. Its particles had an average diameter of 38.82 µm and a specific surface area of 3.02 m2/g. Through microscopic techniques, mainly SEM, starch granules in different shapes and sizes were observed. XRD and FTIR showed structure and functional groups typical of starchy materials, evidencing the ecological character of BMF. Progress is necessary to achieve the continuous and comprehensive use of babassu coconut by-products, mainly BMF, allowing greater appreciation. The characteristics of BMF are of great interest in PLA-based films, as they are green materials and non-toxic to the environment.

Polymeric nanoparticles containing babassu oil: A proposed drug delivery system for controlled release of hydrophilic compounds

Different drug delivery systems are prepared on the nanoscale to improve performance in drug formulations, such as nanoparticles or nanoemulsions. Polymeric nanoparticles have been used to encapsulate drugs for several applications because of some characteristics of these carriers to control drug delivery, transport molecules to a specific tissue, protect the drugs, and increase drug bioavailability. When using nanocapsules, an essential parameter for encapsulating different hydrophilic or lipophilic molecules is the characteristics of the core. Babassu oil (BBS) is a natural product from Brazil, composed majoritary of short-chain saturated fatty acids. BBS has an elevated hydrophilic-lipophilic balance (HLB), which may promote interaction of the oil with hydrophilic drugs. In this study, we developed and characterized particles containing babassu oil, solely or combined with sorbitan monostearate (Span® 60) or medium chain triglycerides (MCT) in the core to test different HLB and evaluated the encapsulation of a model hydrophilic molecule. Different techniques were used to characterize all formulations in terms of size and distribution, and in vitro drug release by dialysis technique was performed. The BBS was also characterized and presented 46,05 ± 1,11% and 15,38 ± 0,06% of lauric and myristic acid, respectively; saponification index of 248.87 ± 0.64 mg of KOH per gram of BBS, and no oxidation of the oil was indicated by means of peroxide index. Evaporation of solvent carried in the room or reduced pressure influenced the particles' size; nevertheless, all had a z-average smaller than 220 nm. Nanoparticles with a ratio among aqueous phase and organic phase of 2.8 were considered adequate to encapsulate diclofenac sodium. The particles size/zeta potential were 189.83 ± 7.86 nm / − 10.39 ± 2.52 mV, 156.80 ± 4.77 nm / − 9.27 ± 4.61 mV, and 168.87 ± 5.22 nm / − 12.98 ± 4.66 mV to nanoparticles prepared with BBS + MCT, BBS, and BBS + Span® 60, respectively. All formulations exhibited an amount of drug content close to the theoretical amount (1.0 mg mL−1), and no difference was observed in the release profile among the three nanoparticles. Formulation containing only babassu oil in the core displayed 66.78 ± 15.62% of encapsulation efficiency to diclofenac sodium, the highest value among all formulations tested. Results demonstrate that the innovative nanoparticles containing BBS promote the encapsulation of a model hydrophilic molecule, and other components can be evaluated to change the core’s hydrophilicity and encapsulation of molecules.

Moisturizing emulsion with Attalea speciosa (babassu) oil as an alternative treatment for cutaneous xerosis in leprosy

This study investigated the moisturizing action of a topical emulsion containing babassu coconut almond oil in xerotic skin volunteers treated or undergoing leprosy treatment. A total of 20 volunteers (16 men and 4 women, with a mean age of 52.2 ± 15.3) with cutaneous xerosis performed the tests after signing the informed consent form. The research subjects were instructed to use the product under investigation (Attalea speciosa almond oil gel-cream formulation) 1 (one) once a day, in two 15-day cycles: first with the gel-cream formulation with babassu oil (treatment A) and the second with the gel-cream formulation without babassu oil (treatment B). The moisturizing emulsion did not significantly alter (p&gt; 0.05) the xerotic skin pH of the volunteers, treatment A (11.6 ± 0.1), treatment B (8.8 ± 0.2) and control (11.4 ± 0.2) at week W7, when compared to week W15, treatment A (11.0 ± 0.4), treatment B (11.6 ± 0.1) and control (11.4 ± 0.2). Clinical evaluations showed the efficacy and good tolerability of topical emulsion in the hydration of xerotic skin. The biophysical evaluation of the moisturizing emulsion proved the significant improvement of skin moisture.

Performance of Eversa Transform 2.0 Lipase in Ester Production Using Babassu Oil (Orbignya sp.) and Tucuman Oil (Astrocaryum vulgar): A Comparative Study between Liquid and Immobilized Forms in Fe3O4 Nanoparticles

In this study, biodiesel was produced through the enzymatic esterification of vegetable oils from two common Brazilian palm trees: babassu and tucuman. The oils were hydrolyzed by a chemical route and their free fatty acids esterified with ethanol and methanol using the lipase enzyme Eversa® Transform 2.0 in free forms and supported in iron magnetic nanoparticles (Fe3O4) (enzymatic load: 80 UpNPBg−1). These enzymatic reactions were performed at an oil–alcohol molar ratio of 1:1, reaction temperature of 37 °C, agitation at 150 rpm, and reaction times of 2, 4, 6 and 8 h for the reactions catalyzed by the soluble enzyme and 8 h for the reactions using the biocatalyst. The conversions of fatty acids in ethyl and methyl esters obtained were monitored by gas chromatography (CG). The results obtained from ester synthesis using enzyme catalysts in free form were better: babassu 52.6% (methanol) and 57.5% (ethanol), and for tucuman 96.7% (methanol) and 93.4% (ethanol). In the case of immobilized enzymes, the results obtained ranged from 68.7% to 82.2% for babassu and from 32.5% to 86.0% for tucuman, with three cycles of reuse and without significant catalyst loss. Molecular coupling studies revealed the structures of lipase and that linoleic acid bonded near the active site of the enzyme with the best free energy of −6.5 Kcal/mol.

Evaluation of Babassu Cake Generated in the Extraction of the Oil as Feedstock for Biofuel Production

The growing demand for energy and the concern about environmental impacts reinforce the necessity for renewable energy sources such as biofuels. In this study, cake generated in the babassu oil extraction was evaluated as a potential feedstock for solid biofuel production, and it contains a blend of cashew nutshell, sugarcane bagasse, carnauba straw, and carnauba stalk. All samples were characterized by proximate analysis and Higher Heating Value. Carbonization was used to improve energy performance and compaction to understand the mechanism and the characteristics of the biomasses compacted. In the extraction of babassu oil, fresh and aged (90 days) kernel samples were used. The fresh samples reached a yield of 59.8%, and the aged samples reached a yield of 70.66%. The carbonization of babassu cake was carried out in a Muffle furnace at temperatures of 250, 300, 350, and 400 °C. The fresh babassu cake showed an HHV of 23.06 MJ kg−1 and after carbonization of 28.07 (250 °C), 30.69 (300 °C), 28.24 (350 °C), and 18.27 MJ kg−1 (400 °C). At 400 °C, a decrease in HHV of 20.8% occurred, and an increase in Ash (%) of 195% occurred. Proximate analysis showed that biomasses are compatible, with some having a higher compatibility than other biological materials already used as fuels in the industry.

Sustainable Fuels for High School Students: Synthesis of Biodiesel from an Amazon Region Oil (Babassu)

We describe here a four-stage project on the synthesis of a sustainable fuel (biodiesel, BD) from an Amazon region oil, namely, babassu oil (BO). The project was carried out both at Humboldt high school (project discussion and evaluation) and at the Institute of Chemistry, the University of São Paulo, ChemUSP (properties of BO; synthesis of BD). The students were asked to discuss aspects (origin, economic and environmental) of (petroleum-based) diesel oil and BD; suggest a candidate oil/fat from the Amazon region and an alcohol for the synthesis of BD, and discuss experimental variables that bear on biofuel yield. The students discussed this assignment in class, as well as what they are going to do during the third (experimental) stage of the project, carried out at ChemUSP. The students determined the acid- and saponification number of BO, synthesized BD using an optimized experimental protocol, prepared mixtures of (BO + authentic BD) and were introduced to gas chromatography. The students evaluated the project positively because of the socioeconomic- and environmental relevance of BD, its production from an Amazon region oil, and their contact with ChemUSP. We recommend this project for high school students because of its simplicity, safety, low cost, and because the school/university contact is beneficial for both sides.

Temperature Effect in the Babassu (Orbignya speciosa) Oil: A Physico-Chemical Study

Babassu (Orbignya speciosa) is a Brazilian palm with extraordinary importance in socioeconomic and ecologic terms. It is found in humid tropical areas, especially in degraded landscapes. There are several uses for babassu coconut and babassu oil. However, their immense potential for large-scale providing other industrial products still needs to be explored due to the necessity for modern scale planning and deep knowledge of vast spectrum thermodynamic properties. This paper gathers a new experimental physico-chemical study of the temperature effect on two critical properties, density and ultrasonic velocity for babassu oil, due to its rising economic significance and a high potential for intensive farming in regions with low economic resources. We consider how accurately different theoretical prediction methods work due to modern processes, design, and algorithm simulations being strongly computer-oriented. The Agrawal-Thodos equation for density and Collision Factor Theory for ultrasonic velocity was selected, mainly attending to ease of use and range of application. We observed a good response at the studied conditions, despite geometrical simplifications into triglyceride molecules and using estimated critical magnitudes by molecular group contribution approach. A broad comparison was made with disposable open literature thermodynamic data, showing an essential dispersion of data, and highlighting the quality of the experimental data presented in this work.

Copaiba Oil Resin Exerts an Additive Effect to Babassu Oil on Behavioral Changes in Human Endometriotic Cell Cultures.

Current drugs for the treatment of endometriosis are not able to completely cure the condition, and significant side effects hinder the continuation of treatment. Therefore, it is necessary to search for new drug candidates. In the present paper, the use of plant extracts is highlighted. Babassu oil and Copaiba oil resin have several therapeutic properties. We investigated the in vitro effects of two nanoemulsions containing oil extracted from Babassu (Orbignya speciosa) nuts (called SNEDDS-18) and/or oil resin extracted from Copaiba trunk (Copaifera langsdorffii) (called SNEDDS-18/COPA) on cultured human eutopic endometrium stromal cells from endometrial biopsies of patients without (CESC) and with (EuESC) endometriosis as well as human stromal cells from biopsies of endometriotic lesions (EctESC). CESC, EuESC, and EctESC were taken and treated with SNEDDS-18 and SNEDDS-18/COPA to evaluate their effects on cytotoxicity, cell morphology, proliferation, and signaling pathways. After 48 h of incubation with SNEDDS-18 and SNEDDS-18/COPA, cell viability and proliferation were inhibited, especially in EctESC. The lowest concentration of both nanoemulsions reduced cell viability and proliferation and broke down the cytoskeleton in EctESCs. After 24 h of treatment a decrease in IL-1, TNF-α, and MCP-1 was observed, as well as an increase in IL-10 production. Both nanoemulsions can affect endometriotic stromal cell behaviors, thus revealing two potential candidates for new phytotherapeutic agents for the management of endometriosis.