This article presents the results of the quantitative determination of fatty acids and vitamins, contained in Acanthophyllum pungens plants, belonging to the Caryophyllaceae family growing in Khantagi, located near the city of Kentau, Turkestan region. As a result of gas chromatography of fatty acids, obtained as a result of studies of plant materials, it has been found that Acanthophyllum pungens contains 87% of unsaturated fatty acids, and 13% of saturated fatty acids. Saturated fatty acids: myristic, palmitic, stearic, pentadecanoic acids, while unsaturated fatty acids: palmitic, oleic, linoleic, and linolenic acids. As a result of quantitative analysis, it has turned out that oleic and linoleic acids are contained in large quantities, and the content of linolenic acid is much lower. It has been established that the plant Acanthophyllum pungens contains water-soluble B3-nicotinic acid, B5-pantothenic acid, B9-folic acid and vitamins A, C, E. It has also been found that the relatively high content of vitamin B5 (pantothenic acid), and the content of vitamins B6, B1, B2 and D are absent altogether. The phytochemical studies of the aboveground part of the Acanthophyllum pungens plant collected during the budding phase have been carried out in accordance with the methodological recommendations of the State Pharmacopoeia of the Republic of Kazakhstan. Using Clarus-600 gas chromatography, the amount of fatty acids (high-carbon acids) contained in the Acanthophyllum pungens plant has been determined. A qualitative analysis has been carried out by paper chromatography (PC) and gas-liquid chromatography (GC). A certain amount of vitamins and fatty acids of the Acanthophyllum pungens plant has been recommended for the first time.