Β-glucan Production Research Articles

Isolation and Characterization of β-Glucan Containing Polysaccharides from Monascus spp. Using Saccharina japonica as Submerged Fermented Substrate

Beta-glucan (β-glucan), a naturally occurring complex polysaccharide, has drawn attention for its diverse health benefits, including immune system modulation. β-glucan was extracted from two fungi, Monascus purpureus (Mp) and Monascus kaoliang (Mk), cultured in Saccharina japonica via submerged fermentation. The yield, solubility, total sugar, reducing sugar, protein content, Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetric analysis (TGA), Scanning Electron Microscopy (SEM), in vitro free radical scavenging activity, and cytotoxicity were analyzed. A significant yield of β-glucans, with the contents of 51.30 ± 1.54% in Mp and 44.24 ± 1.18% in Mk was observed on a dry weight basis. Water solubility slightly varied, measuring 36.25 ± 1.14% in Mp and 31.25 ± 0.94% in Mk. Total sugar and reducing sugar content in Mp and Mk derived β-glucans were 114.75 ± 2.54 mg/g and 100.25 ± 1.86 mg/g, 7.38 ± 0.78 mg/g, and 8.39 ± 0.46 mg/g, respectively. FTIR spectra resembled the standard, and TGA confirmed heat stability. XRD patterns indicated that the extracted β-glucans, including the standard one, showed the most prominent diffraction peaks in the lower 2θ range, suggesting similar crystalline phases; however, they differed in crystallinity and degree of amorphous content. SEM images displayed characteristic rough and fibrous shapes and surfaces for extracted β-glucans but it was uniform and of a regular shape in the standard sample. The isolated β-glucans exhibited in vitro free radical scavenging and no cytotoxicity was observed in the MTS assay. Therefore, utilizing S. japonica as a substrate in the fermentation process by Monascus spp. presents a unique opportunity in the production and utilization of β-glucans.

Polyphasic identification of Rhizopus oryzae and evaluation of physical fermentation parameters in potato starch processing liquid waste for β-glucan production

Β-glucans are polysaccharide macromolecules that can be found in the cell walls of molds, such as Rhizopus oryzae. They provide functional properties in food systems and have immunomodulatory activity, anticancer, and prebiotic effects; reduce triglycerides and cholesterol; and prevent obesity, among others benefits. Furthermore, potato starch production requires a large amount of water, which is usually discharged into the environment, creating problems in soils and bodies of water. The physical parameters to produce β-glucans were determined, liquid waste from potato starch processing was used and native Rhizopus oryzae was isolated and identified from cereal grains. The isolates grew quickly on the three types of agars used at 25 °C and 37 °C, and they did not grow at 45 °C. Rhizopus oryzae M10A1 produced the greatest amount of β-glucans after six days of culture at 30 °C, pH 6, a stirring rate of 150 rpm and a fermentation volume of 250 mL. By establishing the physical fermentation parameters and utilizing the liquid waste from potato starch, Rhizopus oryzae M10A1 yielded 397.50 mg/100 g of β-glucan was obtained.

Development of β-glucan production from microorganisms as active ingredients in cosmeceutical products for skin youthfulness

Development of β-glucan production from microorganisms as active ingredients in cosmeceutical products for skin youthfulness

Enhancement of β-glucan production in two Arthrospira species cultivating in anaerobic digestion effluent of water hyacinth

Arthrospira is an edible cyanobacterium that contains valuable biomolecules, including proteins, carbohydrates, pigments, and β-glucans. This study aimed to determine the biochemical compositions of the two species of Arthrospira cultivated in anaerobic digestion effluent obtained from water hyacinth. This fast-growing aquatic macrophyte causes significant environmental issues worldwide. It’s an urgent issue to confirm the utility of such nuisance macrophyte for sustainable management of freshwater ecosystems. Even with six- and eight-fold diluted effluent containing a limited amount of phosphate, algal biomass production was 0.62 g/L, with higher carbohydrate but lower protein and lipid content than those in the algal cells cultivated with standard media. β-glucan was produced at 4–18% of algal dry mass, being 1.5–7 times higher than those in the standard media. Additionally, phosphate and ammonium removal efficiencies in the cultivation were over 96%. These findings suggest that cultivating Arthrospira using the effluent has promising potential for producing algal material including valuable biomolecules like β-glucans from nuisance macrophytes through sustainable nutrient recycling.

Valorization of spent Brewers yeast in the integrated production of the fungal exopolysaccharide (1 → 6)-β-D-glucan (lasiodiplodan) and single-cell protein

Spent Brewers yeast was studied as a low-cost alternative feedstock for sustainably producing a high value-added microbial β-D-glucan (lasiodiplodan). The potential of using the mycelial biomass (single-cell protein, SCP) generated in the production of β-glucan was also evaluated as a strategy of an integrated biorefining. Five cell lysis protocols were studied to obtain a yeast cell lysate rich in nutrients. Mineral supplementation of the fermentation medium was assessed, and the SCP produced was characterized by the nutritional composition, amino acid, and lipid profiles. Lasiodiplodia theobromae MMPI was effective in the co-production of lasiodiplodan ((1 → 6)-β-D-glucan)) and SCP in a cell lyzate-based medium. Enzymatic lysis with Alcalase promoted more significant cell rupture and release of soluble intracellular components. Highest production of lasiodiplodan (2.12 gL-1) and mycelial biomass (9.95 gL-1) resulted from Alcalase-treated cell lysate-based medium (7 gproteinL−1) supplemented with sucrose (60 gL-1). Under these conditions, volumetric productivities of lasiodiplodan and mycelial biomass: 0.029 gL−1h−1 and 0.138 gL−1h−1, respectively, were obtained. Mycelial biomass generated in this cultivation was rich in protein and contained all essential amino acids, especially high concentrations of leucine (69 mgg−1protein), lysine (62.2 mgg−1protein), valine (44.2 mgg−1protein), threonine (43.2 mgg−1protein). Co-production of lasiodiplodan and SCP could be an economically promising bioprospecting alternative.

Determination the Optimum Conditions for β-glucan Production Extracted from Saccharomyces cerevisiae

Β-glucan is a natural polysaccharide composed of a group of glucose monomers linked by beta glycoside bonds that yeasts can be synthesized. The effect of different temperatures, inoculum size, glucose concentration, yeast extract concentration, pH, and culture media on the β-glucan production from saccharomyces cerevisiae was studied. In addition, the dry weight of biomass containing β-glucan was measured. The results showed that the optimum conditions for β-glucan production were when the cells were grown at 30 ºc for 48 hours and pH = 7 with 10 % inoculum size, 2% glucose, and 0.1% yeast extract. Furthermore, the best culture medium for β-glucan production was yeast extract-glucose medium with a dry weight of glucan 2.5mg/5ml. In addition, this study aimed to determine the best method for β-glucan extraction with acceptable purity and concentration. Β-glucan was extracted using two methods autolysis and alkali-acids extraction. The dried and weighed crude β-glucan was 6 gm/l, and 8 gm/l for autolysis of yeast cells, and alkali-acids extraction, respectively. The analysis of glucan by FT-IR and HPLC showed that the method of extraction by autolysis of yeast cells was successful to obtain β-glucan without the presence of the α-glucan with a concentration of β-glucan 10.8mg/gm, while in the extraction by alkali-acid were obtained mixtures of α-glucan and β-glucan with the concentration of β-glucan 7.4 mg/gm. The current study revealed that the optimum conditions of β-glucan production and the best culture medium for β-glucan production was yeast extract-glucose medium and the best method for β-glucan extraction was autolysis extraction.

The effect of nitrogen limitation on carbohydrates and β-glucan accumulation in Nannochloropsis oculata

Nitrogen (N) is the second most abundant element in microalgae biomass, following carbon. Microalgae have a high carbon and nitrogen metabolism system, making nitrogen limitation (N-limitation) a crucial factor that modifies their biochemical composition. In Nannochloropsis genus cultures, N-limitation can cause lipid or carbohydrate accumulation. This research aims to evaluate the effect of N-limitation on carbohydrate and β-glucan accumulation in Nannochloropsis oculata during maximum biomass production (i.e., finished logarithmic phase and early start stationary phase in batch culture) in the two-stage process context. To achieve this goal, we tested the effect of five levels of N availability (treatments 100, 75, 50, 25, and 0 % of N, concerning F medium) in batch culture using sodium nitrate (NaNO3) as the N source. The initial availability of N in each treatment was 1.7, 1.3, 0.8, 0.4, and 0 mmol∙l−1. The results showed that the highest concentration of N. oculata biomass, carbohydrate, and β-glucans content was produced during the logarithmic phase at 0.8 and 1.32 mmol∙l−1 N initial batch culture concentration (50 and 75 % N treatments), without a significant difference (P > 0.05) from the F medium used. Total carbohydrates obtained were 15.2 %, 19.7 %, 17.2 %, 12.7 %, and 14.2 % (dry weight) for the 100 %, 75 %, 50 %, 25 %, and 0 % N treatments, respectively. Similarly, the β-glucans in microalgal biomass were 5.0 %, 7.4 %, 6.7 %, 4.4 %, and 1.7 % (dry weight) for treatments 100 %, 75 %, 50 %, 25 %, and 0 % N, respectively. The 25 % and 0 % N treatments had a negative effect (P < 0.05) on cell population growth, carbohydrates, and β-glucans accumulation. Based on these results, we recommended conducting studies on the effect of N-limitation in N. oculata culture primarily at 50 % and 75 % N treatments, during the stationary phase and/or second stage of the two-stage process context, such as a chronic or and long-term effect, along with a partial supply of N or testing other inductors might also be explored. This knowledge can help to optimize the industrial production of microalgae β-glucans.

Optimizing the synthesis of yeast Beta-glucan via response surface methodology for nanotechnology application

BackgroundThe production of biopolymers from waste resources is a growing trend, especially in high-population countries like Egypt. Beta-glucan (β-glucan) belongs to natural polysaccharides that are derived from plant and microbial origins. In this study, following increasing demands for β-glucan owing to its bioactive properties, a statistical model to enhance microbial β-glucan production was evaluated for its usefulness to the food and pharmaceutical industries. In addition, a trial to convert β-glucan polymer to nanostructure form was done to increase its bioactivity.ResultsIngredients of low-cost media based on agro-industrial wastes were described using Plackett–Burman and central composite design of response surface methodology for optimizing yeast β-glucan. Minerals and vitamin concentrations significantly influenced β-glucan yield for Kluyveromyces lactis and nitrogen and phosphate sources for Meyerozyma guilliermondii. The maximum predicted yields of β-glucan recovered from K. lactis and M. guilliermondii after optimizing the medium ingredients were 407 and 1188 mg/100 ml; respectively. For the first time, yeast β-glucan nanoparticles (βGN) were synthesized from the β-glucan polymer using N-dimethylformamide as a stabilizer and characterized using UV–vis spectroscopy, transmission electron microscope (TEM), dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FT-IR). The average size of βGN was about 300 nm as determined by DLS. The quantitative variation of functional groups between β-glucan polymer and βGN was evaluated by FT-IR for explaining the difference in their biological activity against Normal Homo sapiens-Hela contaminant and Hepatic cancer cell lines.ConclusionsEnriching the low-cost media based on agro-industrial wastes with nutritional ingredients improves the yield of yeast β-glucan. The present study succeeds to form β-glucan nanoparticles by a simple method.

Effect of Solid-State Fermented Brown Rice Extracts on 3T3-L1 Adipocyte Differentiation.

Aspergillus oryzae KCCM 11372 was used to enhance the production of β-glucan using humidity control strategies. Under conditions of 60% humidity, solid-state fermentation (SSF) increased the yields of enzymes (amylase and protease), fungal biomass (ergosterol), and β-glucan. The maximum concentrations obtained were 14800.58 U/g at 72 h, 1068.14 U/g at 120 h, 1.42 mg/g at 72 h, and 12.0% (w/w) at 72 h, respectively. Moreover, the β-glucan containing fermented brown rice (β-glucan-FBR) extracts at concentrations of 25-300 μg/ml was considered noncytotoxic to 3T3-L1 preadipocytes. We then studied the inhibitory effects of the extracts on fat droplet formation in 3T3-L1 cells. As a result, 300 μg/ml of β-glucan-FBR extracts showed a high inhibition of 38.88% in lipid accumulation. Further, these extracts inhibited adipogenesis in the 3T3-L1 adipocytes by decreasing the expression of C/EBPα, PPARγ, aP2, and GLUT4 genes.

Effect of enzymatic hydrolysis and nitrogen on Saccharomyces cerevisiae β-glucan production from Manihot utilissima and Maranta arunadinacea waste

This experiment utilised cassava (Manihot utillissima) and arrowroot (Maranta arunadinacea) wastes as the medium of propagation of Saccharomyces cerevisiae to produce β-glucan. The amyloglucosidase hydrolysed the waste, followed by fermentation in the nitrogenous medium by S. cerevisiae. The β-glucan pellet was extracted using 2% NaOH alkaline solution at 90°C for 5 hours, followed by a series of centrifugation processes. The highest glucose concentration from hydrolysis resulted from adding 57.5 mg amyloglucosidase enzyme for arrowroot waste with 95.93% conversion and 50 mg enzyme for cassava waste with 64.70% conversion. The highest amount was obtained for producing S. cerevisiae by adding 4.75 g peptone to all samples. The optimum number of cells was obtained at 1.61 x 108 colonies at t = 48 hours for arrowroot waste and 8.55 x 107 colonies at t = 48 hours for cassava waste. For β-glucan production, the highest number was obtained by using 3.99 g of peptone for cassava waste with a yield of 1.20% and by using 4.75 g of peptone for arrowroot waste with a yield of 1.23%. For β-glucan pellet, the highest number was 1.77 g L-1 (0.18 % b/v) from cassava waste medium and 1.91 g L-1 (0.19% b/v) from arrowroot waste. Mutant cells in the Yeast Extract–Peptone–Glycerol (YPG) medium produced 6.56 g L-1 (0.66% b/v) β-glucan pellet, while wild-type cells in the similar medium produced 1.84 g L-1 (0.18% b/v).

Food By-Products and Agro-Industrial Wastes as a Source of β-Glucans for the Formulation of Novel Nutraceuticals.

Food and agro-industrial by-products provoke a great environmental and economic impact that must be minimized by adding value to these wastes within the framework of circular economy. The relevance of β-glucans obtained from natural sources (cereals, mushrooms, yeasts, algae, etc.), in terms of their interesting biological activities (hypocholesterolemic, hypoglycemic, immune-modulatory, antioxidant, etc.), has been validated by many scientific publications. Since most of these by-products contain high levels of these polysaccharides or can serve as a substrate of β-glucan-producing species, this work reviewed the scientific literature, searching for studies that utilized food and agro-industrial wastes to obtain β-glucan fractions, attending to the applied procedures for extraction and/or purification, the characterization of the glucans and the tested biological activities. Although the results related to β-glucan production or extraction using wastes are promising, it can be concluded that further research on the glucans' characterization, and particularly on the biological activities in vitro and in vivo (apart from antioxidant capacity), is required to reach the final goal of formulating novel nutraceuticals based on these molecules and these raw materials.

ULTRASOUND-ASSISTED AND ENZYMATIC-BASED METHOD FOR ISOLATION OF β-GLUCANS FROM OAT BRAN

β-Glucans are a group of non-starchy polysaccharides, or (1,3),(1,4)-β-D-glucans, that can be found in the cell walls of several species of bacteria, algae, lichens, fungi, and cereal grains. These carbohydrates are extensively used in food industry, cosmetics, pharmaceuticals and healthcare, therefore optimization of the extraction and isolation of β-glucans from grain sources has an especial importance in various fields of biotechnology, drug design, food science and technology. The aim of the study was to develop an optimized technological scheme for isolation of β-glucans from oat bran based on ultrasonic and enzymatic processing of raw material. Materials and methods. β-Glucans were isolated from grinded oat cereals during multi-stage process, which includes extraction of grain fats, hydrobarothermic processing, ultrasonification, enzymatic hydrolysis of concomitant starch and proteins, precipitation of β-glucan fraction by ethanol, centrifugation, and dry-freezing. Yield of β-glucans from raw material and its concentration in the final product were determined after hydrolysis by sulfuric acid or enzymatic cleavage by endo-1,3(4)-β-glucanase. Results. As shown by acidic hydrolysis of the final product, the yield of β-glucans was 10.8 ± 0.23% and concentration was 79.6 ± 3.89%, while enzymatic hydrolysis gave 8.7 ± 0.82% and 65.1 ± 4.72%, respectively. Thus, the use of hydrobarothermic and ultrasound pre-treatment of raw material in combination with proteolytic digestion of ballast lipids and proteins allowed producing oat β-glucans in amounts comparable with those in case of acid- or alkali-based procedures. Conclusions. The described technological scheme of β-glucan isolation from oat bran based on sequential hydrobarothermic processing, ultrasonification, and enzymatic removing starch and proteins can be widely used for routine β-glucan production for various purposes in food technology, pharmacological industry, and medicine.

Effects of β-glucan on Salmonella enterica serovar Typhimurium swine colonization and microbiota alterations

BackgroundThe 2017 Veterinary Feed Directive eliminated the use of medically important antibiotics for growth promotion of food animals; thus, alternative growth promoters are highly desirable by food animal producers to enhance animal health and reduce pathogen colonization, including the human foodborne pathogen Salmonella. β(1-3)(1-6)-d-glucan (β-glucan) is a soluble fiber with prebiotic characteristics; it has been shown to modulate immune and intestinal functions that strengthen swine resistance to health challenges such as bacterial infections when supplemented in the diets of growing pigs. The current study evaluated the effects of a β-glucan product on gut microbial community structure as well as Salmonella shedding and intestinal colonization.ResultsFive-week-old pigs were fed a β-glucan amended diet at 500 g/ton (n = 13) or a non-amended control diet (n = 14) for three weeks, followed by inoculation of the 27 pigs with 1 × 109 colony forming units of Salmonella enterica serovar Typhimurium strain UK1. While remaining on the respective diets, fecal samples collected at 2, 4, 7, and 16 days post-inoculation (dpi) were similar for Salmonella shedding counts between the two diets. At 16 dpi, Salmonella counts were significantly lower in the cecal contents of the β-glucan-fed pigs (P = 0.0339) and a trend towards a reduction was observed in the Peyer’s patches region of the ileum (P = 0.0790) compared to the control pigs. Pigs fed β-glucan for three weeks exhibited an increase in members of the Clostridia class in their fecal microbial communities, and after inoculation with Salmonella, several potentially beneficial microorganisms were enriched in the microbiota of β-glucan-fed pigs (Lactobacillus, Ruminococcaceae, Prevotellaceae, Veillonellaceae, Bifidobacterium and Olsenella).ConclusionAdministration of β-glucan altered the swine gut microbiome and reduced Salmonella colonization in the cecal contents.

Aspergillus niger 를 이용한 곡류발효물의 배양특성 및 α-Glucosidase 활성 저해효과

Liquid-state fermentation (LSF) and solid-state fermentation (SSF) were carried out to produce enzymes and β-glucan. The modified synthetic medium composition in liquid-state fermentation (LSF) was determined to be 5% (w/v) whole oat flour, 2% (w/v) tryptone, and 2% (w/v) rice bran. The different humidity conditions of oat by solid-state fermentation were evaluated in terms of maximum production of fungal biomass, amylase, protease, and β-glucan, which were 0.23 mg/g, 7,157.38 U/g, 413.67 U/g, and 8.26% (w/w), respectively, at 60% of humidity condition. Moreover, α-glucosidase inhibition activities of fermented oat with 60% humidity at 48 h were shown to be high 49.08% (w/w). Therefore, liquid- and solid-state fermentation processes were found to enhance the overall fermentation yields of oat to produce enzymes and β-glucan.

Screening and Molecular Identification of Lactic Acid Bacteria Producing β-Glucan in Boza and Cider

The goal of this study was screening and molecular identification of Lactic Acid Bacteria (LAB) producing β-glucan from different species isolated from boza and cider compared to a standard strain for Lactobacillus rhamnosus NRRL 1937 (LGG). From 48 unknown isolates, four LAB strains were selected. Based on the NCBI database, their nomenclature was A3, B6, and C9 for Limosilactobacillus fermentum SH1, SH2, and SH3 along with D6 for Leuconostoc mesenteroides SH4. Also, their similarity values were 100%, 99.8%, 100%, and 100%, respectively. The potential of Exopolysaccharide (EPS) (as β-glucan) production for selected LAB strains by gtf gene, conventional PCR and gene expression using both LGG as a control and LAB 16S rRNA gene as a house-keeping gene was investigated. In addition, EPS (mg/100 mL), cell mass (mg/100 mL), pH, total carbohydrate%, total protein% and β-glucan% by the HPLC for all selected LAB isolates were studied. All results of genetic and chemical tests proved the superiority of B6 treatment for L. fermentum SH2. The results showed the superiority of B6 treatment in gtf gene expression (14.7230 ± 0.070-fold) followed by C9 and A3 treatments, which were 10.1730 ± 0.231-fold and 8.6139 ± 0.320-fold, respectively. while D6 treatment recorded the lowest value of gene expression (0.8566 ± 0.040-fold) compared to the control LGG (one-fold). The results also demonstrated that B6 treatment was superior to the other treatments in terms of EPS formation, with a value of 481 ± 1.00 mg/100 mL, followed by the C9 treatment at 440 ± 2.00 mg/100 mL, compared to the LGG (control) reaching 199.7 ± 3.51 mg/100 mL. Also, the highest % of quantitative and qualitative β-glucan in EPS was observed in B6 followed by C9, D6 and A3 which were 5.56 ± 0.01%, 4.46 ± 0.01%, 0.25 ± 0.008% and 0.12 ± 0.008%, respectively compared to control (0.31 ± 0.01%). Finally, the presented results indicate the importance of screening the local LAB isolates to obtain a superior strain for β-glucan production which will be introduced in a subsequent study under optimum conditions.

Production of prebiotic aubasidan-like β-glucan from Aureobasidium thailandense NRRL 58543 and its potential as a functional food additive in gummy jelly

To enhance the yield of Aureobasidium thailandense NRRL 58543 β-glucan, effects of medium ingredients and their optimal concentrations were investigated. The highest yield of β-glucan (37.73 g/L) was obtained in the medium containing sucrose, NaNO3 and sunflower oil at concentrations of 60 g/L, 0.6 g/L, and 81.7 mL/L, respectively. The low molecular weight (LMW) β-glucan (78 kDa) was assessed its prebiotic property in comparison to the native one (>410 kDa). The growth of the probiotics, Lactobacillus casei and L. brevis, was significantly enhanced, although a higher growth was observed in the medium containing the native β-glucan. In addition, the native β-glucan was resistant with simulated salivary and gastrointestinal juice. The addition of β-glucan (2.5 and 5 g/L) in gummy jellies resulted in the increase of the color intensity, hardness, gumminess, and chewiness, while the springiness and cohesiveness decreased compared with the control. The highest score of overall acceptability (7.83 ± 0.75) according to 9-point hedonic scale was obtained from the gummy jellies with 0.5% β-glucan that could be stored at room temperature for seven days without microbial growth. Our findings suggest that A. thailandense β-glucan exhibits an immense potential in the food industry as a functional ingredient and texturing agent.

Analysis of Biochemical and Genetic Variability of Pleurotus ostreatus Based on the β-Glucans and CDDP Markers.

Oyster mushroom (Pleurotus ostreatus) is still one of the most cultivated edible and medicinal mushrooms. Despite its frequent cultivation around the world, there is currently just a little information available on the variability of strains in terms of the content of β-glucans in them. This work presents an extensive study of 60 strains in terms of the content of α-glucans and β-glucans in their caps and stipes. The authenticity of the production strains based on an analysis of the variability of their genome by CDDP (Conserved DNA-derived polymorphism) markers was confirmed, whereas identical CDDP profiles were identified between samples 45, 89, 95, and 96. Genetic variability of the analyzed production strains showed a high polymorphism and effective discriminative power of the used marking technique. Medium positive correlations were found among the CDDP profiles and β-glucan content in the group of strains that generated the same CDDP profiles, and low negative correlation was found among these profiles in the group of low β-glucan content strains. For the determination of glucans content, Mushroom and Yeast analytical enzymatic kit (Megazyme, Bray, Co. Wicklow, Ireland) were used. The results clearly showed that the stipe contains on average 33% more β-glucans than the cap. The minimum detected β-glucan content in the stipe was in strain no. 72, specifically 22%, and the maximum in strain no. 43, specifically 56%, which after the conversion represents a difference of 155%. From the point of view of β-glucan content, the stated strain no. 43 appears to be very suitable for the commercial production of β-glucans under certain conditions.

Use of the β-Glucan-Producing Lactic Acid Bacteria Strains Levilactobacillus brevis and Pediococcus claussenii for Sourdough Fermentation-Chemical Characterization and Chemopreventive Potential of In Situ-Enriched Wheat and Rye Sourdoughs and Breads.

The aim of the present study was to examine β-glucan production and the potential prebiotic and chemopreventive effects of wheat and rye sourdoughs and breads generated with wild-type and non-β-glucan-forming isogenic mutant strains of Levilactobacillus brevis and Pediococcus claussenii. Sourdough and bread samples were subjected to in vitro digestion and fermentation. Fermentation supernatants (FS) and pellets (FP) were analyzed (pH values, short-chain fatty acids (SCFA), ammonia, bacterial taxa) and the effects of FS on LT97 colon adenoma cell growth, viability, caspase-2 and -3 activity, genotoxic and antigenotoxic effects and on gene and protein expression of p21, cyclin D2, catalase and superoxide dismutase 2 (SOD2) were examined. Concentrations of SCFA were increased and concentrations of ammonia were partly reduced in the FS. The relative abundance of Bifidobacteriaceae was increased in all FPs. Treatment with FS reduced the growth and viability of LT97 cells and significantly increased caspase-2 and -3 activities without exhibiting genotoxic or antigenotoxic effects. The p21 mRNA and protein levels were increased while that of cyclin D2 was reduced. Catalase and SOD2 mRNA and protein expression were marginally induced. The presented results indicate a comparable chemopreventive potential of wheat and rye sourdoughs and breads without an additional effect of the formed β-glucan.

Comparison of Liquid and Solid-State Fermentation Processes for the Production of Enzymes and Beta-Glucan from Hulled Barley.

Solid-state fermentation using hulled barley was carried out to produce enzymes and β-glucan. The one-factor-at-a-time experiments were carried out to determine the optimal composition of the basal medium. The modified synthetic medium composition in liquid-state fermentation was determined to be 70 g/l hulled barley, 0 g/l rice bran, 5 g/l soytone, and 6 g/l ascorbic acid. Optimal pretreatment conditions of hulled barley by solid-state fermentation were evaluated in terms of maximum production of fungal biomass, amylase, protease, and β-glucan, which were 1.26 mg/g, 31310.34 U/g, 2614.95 U/g, and 14.6% (w/w), respectively, at 60 min of pretreatment condition. Thus, the solid-state fermentation process was found to enhance the overall fermentation yields of hulled barley to produce high amounts of enzymes and β-glucan.

Efficiency of β-glucan production by Sparassis crispa depends on mycelium shape

Efficiency of β-glucan production by Sparassis crispa depends on mycelium shape