Azima tetracantha is a shrub known for its various therapeutic properties. Genetic diversity was analysed using RAPD primers among six accessions collected from different locations of Tamilnadu, India. Genetic distances were calculated using Nei's coefficient. Dendrogram was constructed on the basis of the similarity matrix data by unweighted pair group method with average (UPGMA) cluster analysis. The analysis with RAPD markers revealed wide variation within A. tetracantha that reflected a high level of diversity within this species.